Overexpression of malic enzyme in the larval stage extends Drosophila lifespan
[Display omitted] •Overexpression of malic enzyme at larval period extends adult lifespan.•Larval overexpression of malic enzyme increases ROS production.•Malic enzyme overexpression induces an adaptive response in larvae.•Adults exposed to larval overexpression of malic enzyme are resistant to ROS...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2015-01, Vol.456 (2), p.676-682 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Kim, Gye-Hyeong Lee, Young-Eun Lee, Gun-Ho Cho, Youn-Ho Lee, Young-Nam Jang, Yeogil Paik, Donggi Park, Joong-Jean |
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•Overexpression of malic enzyme at larval period extends adult lifespan.•Larval overexpression of malic enzyme increases ROS production.•Malic enzyme overexpression induces an adaptive response in larvae.•Adults exposed to larval overexpression of malic enzyme are resistant to ROS stress.•GstD2 and Trx-2 levels are higher in adults exposed to larval Men overexpression.
Metabolic modifications during the developmental period can extend longevity. We found that malic enzyme (Men) overexpression during the larval period lengthened the lifespan of Drosophila. Men overexpression by S106-GeneSwitch-Gal4 driver increased pyruvate content and NADPH/NADP+ ratio but reduced triglyceride, glycogen, and ATP levels in the larvae. ROS levels increased unexpectedly in Men-overexpressing larvae. Interestingly, adults exposed to larval Men-overexpression maintained ROS tolerance with enhanced expression levels of glutathione-S-transferase D2 and thioredoxin-2. Our results suggest that metabolic changes mediated by Men during development might be related to the control of ROS tolerance and the longevity of Drosophila. |
| doi_str_mv | 10.1016/j.bbrc.2014.12.020 |
| format | Article |
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•Overexpression of malic enzyme at larval period extends adult lifespan.•Larval overexpression of malic enzyme increases ROS production.•Malic enzyme overexpression induces an adaptive response in larvae.•Adults exposed to larval overexpression of malic enzyme are resistant to ROS stress.•GstD2 and Trx-2 levels are higher in adults exposed to larval Men overexpression.
Metabolic modifications during the developmental period can extend longevity. We found that malic enzyme (Men) overexpression during the larval period lengthened the lifespan of Drosophila. Men overexpression by S106-GeneSwitch-Gal4 driver increased pyruvate content and NADPH/NADP+ ratio but reduced triglyceride, glycogen, and ATP levels in the larvae. ROS levels increased unexpectedly in Men-overexpressing larvae. Interestingly, adults exposed to larval Men-overexpression maintained ROS tolerance with enhanced expression levels of glutathione-S-transferase D2 and thioredoxin-2. Our results suggest that metabolic changes mediated by Men during development might be related to the control of ROS tolerance and the longevity of Drosophila.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2014.12.020</identifier><identifier>PMID: 25511696</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adenosine Triphosphate - metabolism ; Animals ; Drosophila ; Drosophila melanogaster - enzymology ; Drosophila melanogaster - genetics ; Drosophila melanogaster - growth & development ; Drosophila Proteins - biosynthesis ; Drosophila Proteins - genetics ; Glutathione Transferase - metabolism ; Glycogen - metabolism ; Larva - enzymology ; Larva - genetics ; Larva - growth & development ; Longevity ; Longevity - genetics ; Longevity - physiology ; Malate Dehydrogenase - biosynthesis ; Malate Dehydrogenase - genetics ; Malic enzyme ; NADP - metabolism ; Pyruvate ; Pyruvic Acid - metabolism ; Reactive Oxygen Species - metabolism ; ROS ; Thioredoxins - metabolism ; Triglycerides - metabolism</subject><ispartof>Biochemical and biophysical research communications, 2015-01, Vol.456 (2), p.676-682</ispartof><rights>2014 Elsevier Inc.</rights><rights>Copyright © 2014 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-2a0971aaa33b4ba72372062fb0b7483312e9efe65cbeea8ab55517d162ed13b43</citedby><cites>FETCH-LOGICAL-c459t-2a0971aaa33b4ba72372062fb0b7483312e9efe65cbeea8ab55517d162ed13b43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2014.12.020$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25511696$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Gye-Hyeong</creatorcontrib><creatorcontrib>Lee, Young-Eun</creatorcontrib><creatorcontrib>Lee, Gun-Ho</creatorcontrib><creatorcontrib>Cho, Youn-Ho</creatorcontrib><creatorcontrib>Lee, Young-Nam</creatorcontrib><creatorcontrib>Jang, Yeogil</creatorcontrib><creatorcontrib>Paik, Donggi</creatorcontrib><creatorcontrib>Park, Joong-Jean</creatorcontrib><title>Overexpression of malic enzyme in the larval stage extends Drosophila lifespan</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>[Display omitted]
•Overexpression of malic enzyme at larval period extends adult lifespan.•Larval overexpression of malic enzyme increases ROS production.•Malic enzyme overexpression induces an adaptive response in larvae.•Adults exposed to larval overexpression of malic enzyme are resistant to ROS stress.•GstD2 and Trx-2 levels are higher in adults exposed to larval Men overexpression.
Metabolic modifications during the developmental period can extend longevity. We found that malic enzyme (Men) overexpression during the larval period lengthened the lifespan of Drosophila. Men overexpression by S106-GeneSwitch-Gal4 driver increased pyruvate content and NADPH/NADP+ ratio but reduced triglyceride, glycogen, and ATP levels in the larvae. ROS levels increased unexpectedly in Men-overexpressing larvae. Interestingly, adults exposed to larval Men-overexpression maintained ROS tolerance with enhanced expression levels of glutathione-S-transferase D2 and thioredoxin-2. Our results suggest that metabolic changes mediated by Men during development might be related to the control of ROS tolerance and the longevity of Drosophila.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>Drosophila</subject><subject>Drosophila melanogaster - enzymology</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophila melanogaster - growth & development</subject><subject>Drosophila Proteins - biosynthesis</subject><subject>Drosophila Proteins - genetics</subject><subject>Glutathione Transferase - metabolism</subject><subject>Glycogen - metabolism</subject><subject>Larva - enzymology</subject><subject>Larva - genetics</subject><subject>Larva - growth & development</subject><subject>Longevity</subject><subject>Longevity - genetics</subject><subject>Longevity - physiology</subject><subject>Malate Dehydrogenase - biosynthesis</subject><subject>Malate Dehydrogenase - genetics</subject><subject>Malic enzyme</subject><subject>NADP - metabolism</subject><subject>Pyruvate</subject><subject>Pyruvic Acid - metabolism</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>ROS</subject><subject>Thioredoxins - metabolism</subject><subject>Triglycerides - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtLxDAURoMoOj7-gAvJ0k3rvWmbtuBGfIPoRsFdSNJbzdCXSWdQf70dRl2Kq2zOd7g5jB0ixAgoT-axMd7GAjCNUcQgYIPNEEqIBEK6yWYAICNR4vMO2w1hDoCYynKb7YgsQ5SlnLH7hyV5eh88heD6jvc1b3XjLKfu86Ml7jo-vhJvtF_qhodRvxCn95G6KvAL34d-eHWN5o2rKQy622dbtW4CHXy_e-zp6vLx_Ca6e7i-PT-7i2yalWMkNJQ5aq2TxKRG5yLJBUhRGzB5WiQJCiqpJplZQ6QLbbLp4LxCKajCaZLsseO1d_D924LCqFoXLDWN7qhfBIVSFmLlLP6BppiKvChxQsUatdPPgqdaDd612n8oBLVKruZqlVytkisUako-jY6-_QvTUvU7-Wk8AadrgKYgS0deBeuos1Q5T3ZUVe_-8n8BdT2R_Q</recordid><startdate>20150109</startdate><enddate>20150109</enddate><creator>Kim, Gye-Hyeong</creator><creator>Lee, Young-Eun</creator><creator>Lee, Gun-Ho</creator><creator>Cho, Youn-Ho</creator><creator>Lee, Young-Nam</creator><creator>Jang, Yeogil</creator><creator>Paik, Donggi</creator><creator>Park, Joong-Jean</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SS</scope></search><sort><creationdate>20150109</creationdate><title>Overexpression of malic enzyme in the larval stage extends Drosophila lifespan</title><author>Kim, Gye-Hyeong ; Lee, Young-Eun ; Lee, Gun-Ho ; Cho, Youn-Ho ; Lee, Young-Nam ; Jang, Yeogil ; Paik, Donggi ; Park, Joong-Jean</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-2a0971aaa33b4ba72372062fb0b7483312e9efe65cbeea8ab55517d162ed13b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Animals</topic><topic>Drosophila</topic><topic>Drosophila melanogaster - enzymology</topic><topic>Drosophila melanogaster - genetics</topic><topic>Drosophila melanogaster - growth & development</topic><topic>Drosophila Proteins - biosynthesis</topic><topic>Drosophila Proteins - genetics</topic><topic>Glutathione Transferase - metabolism</topic><topic>Glycogen - metabolism</topic><topic>Larva - enzymology</topic><topic>Larva - genetics</topic><topic>Larva - growth & development</topic><topic>Longevity</topic><topic>Longevity - genetics</topic><topic>Longevity - physiology</topic><topic>Malate Dehydrogenase - biosynthesis</topic><topic>Malate Dehydrogenase - genetics</topic><topic>Malic enzyme</topic><topic>NADP - metabolism</topic><topic>Pyruvate</topic><topic>Pyruvic Acid - metabolism</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>ROS</topic><topic>Thioredoxins - metabolism</topic><topic>Triglycerides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Gye-Hyeong</creatorcontrib><creatorcontrib>Lee, Young-Eun</creatorcontrib><creatorcontrib>Lee, Gun-Ho</creatorcontrib><creatorcontrib>Cho, Youn-Ho</creatorcontrib><creatorcontrib>Lee, Young-Nam</creatorcontrib><creatorcontrib>Jang, Yeogil</creatorcontrib><creatorcontrib>Paik, Donggi</creatorcontrib><creatorcontrib>Park, Joong-Jean</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Entomology Abstracts (Full archive)</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Gye-Hyeong</au><au>Lee, Young-Eun</au><au>Lee, Gun-Ho</au><au>Cho, Youn-Ho</au><au>Lee, Young-Nam</au><au>Jang, Yeogil</au><au>Paik, Donggi</au><au>Park, Joong-Jean</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of malic enzyme in the larval stage extends Drosophila lifespan</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2015-01-09</date><risdate>2015</risdate><volume>456</volume><issue>2</issue><spage>676</spage><epage>682</epage><pages>676-682</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>[Display omitted]
•Overexpression of malic enzyme at larval period extends adult lifespan.•Larval overexpression of malic enzyme increases ROS production.•Malic enzyme overexpression induces an adaptive response in larvae.•Adults exposed to larval overexpression of malic enzyme are resistant to ROS stress.•GstD2 and Trx-2 levels are higher in adults exposed to larval Men overexpression.
Metabolic modifications during the developmental period can extend longevity. We found that malic enzyme (Men) overexpression during the larval period lengthened the lifespan of Drosophila. Men overexpression by S106-GeneSwitch-Gal4 driver increased pyruvate content and NADPH/NADP+ ratio but reduced triglyceride, glycogen, and ATP levels in the larvae. ROS levels increased unexpectedly in Men-overexpressing larvae. Interestingly, adults exposed to larval Men-overexpression maintained ROS tolerance with enhanced expression levels of glutathione-S-transferase D2 and thioredoxin-2. Our results suggest that metabolic changes mediated by Men during development might be related to the control of ROS tolerance and the longevity of Drosophila.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>25511696</pmid><doi>10.1016/j.bbrc.2014.12.020</doi><tpages>7</tpages></addata></record> |
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| subjects | Adenosine Triphosphate - metabolism Animals Drosophila Drosophila melanogaster - enzymology Drosophila melanogaster - genetics Drosophila melanogaster - growth & development Drosophila Proteins - biosynthesis Drosophila Proteins - genetics Glutathione Transferase - metabolism Glycogen - metabolism Larva - enzymology Larva - genetics Larva - growth & development Longevity Longevity - genetics Longevity - physiology Malate Dehydrogenase - biosynthesis Malate Dehydrogenase - genetics Malic enzyme NADP - metabolism Pyruvate Pyruvic Acid - metabolism Reactive Oxygen Species - metabolism ROS Thioredoxins - metabolism Triglycerides - metabolism |
| title | Overexpression of malic enzyme in the larval stage extends Drosophila lifespan |
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