Ultrasensitive fluorescent proteins for imaging neuronal activity

Fluorescent calcium sensors are widely used to image neural activity. Using structure-based mutagenesis and neuron-based screening, we developed a family of ultrasensitive protein calcium sensors (GCaMP6) that outperformed other sensors in cultured neurons and in zebrafish, flies and mice in vivo ....

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Veröffentlicht in:	Nature (London) 2013-07, Vol.499 (7458), p.295-300
Hauptverfasser:	Chen, Tsai-Wen, Wardill, Trevor J., Sun, Yi, Pulver, Stefan R., Renninger, Sabine L., Baohan, Amy, Schreiter, Eric R., Kerr, Rex A., Orger, Michael B., Jayaraman, Vivek, Looger, Loren L., Svoboda, Karel, Kim, Douglas S.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1888/2249 Action Potentials Animals Biological and medical sciences Calcium - metabolism Calcium-Binding Proteins - chemistry Calcium-Binding Proteins - genetics Cells, Cultured Danio rerio Dendritic Spines - metabolism Eye and associated structures. Visual pathways and centers. Vision Fluorescent Dyes - chemistry Fluorescent proteins Fundamental and applied biological sciences. Psychology GABAergic Neurons - metabolism Humanities and Social Sciences Insects Luminescent Proteins - chemistry Luminescent Proteins - genetics Mice Molecular Imaging multidisciplinary Mutagenesis Mutation Neuroimaging Neurology Neurons Properties Protein Engineering Proteins Pyramidal Cells - metabolism Pyramidal Cells - physiology Science Sensors Spine Vertebrates: nervous system and sense organs Visual Cortex - cytology Visual Cortex - physiology
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