Morphine and buprenorphine do not alter leukocyte cytokine production capacity, early apoptosis, or neutrophil phagocytic function in healthy dogs
•We evaluated the effects of opioids on innate immune function in healthy dogs.•Opioids do not significantly affect leukocyte stimulated cytokine production.•Opioids do not significantly affect early leukocyte apoptosis or phagocytic function.•There is a potential association between hospital stress...
Gespeichert in:
| Veröffentlicht in: | Research in veterinary science 2015-04, Vol.99, p.70-76 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 76 |
|---|---|
| container_issue | |
| container_start_page | 70 |
| container_title | Research in veterinary science |
| container_volume | 99 |
| creator | Monibi, Farrah A. Dodam, John R. Axiak-Bechtel, Sandra M. Amorim, Juliana Zhang, Yan Tsuruta, Kaoru Mann, F.A. DeClue, Amy E. |
| description | •We evaluated the effects of opioids on innate immune function in healthy dogs.•Opioids do not significantly affect leukocyte stimulated cytokine production.•Opioids do not significantly affect early leukocyte apoptosis or phagocytic function.•There is a potential association between hospital stress and cytokine production.•Dogs could be less sensitive to immunomodulatory effects of acute opioid treatment.
Opioids have immunomodulatory properties in many species, but there is little information pertaining to these properties in dogs. Our objective was to compare the in vivo effects of morphine, buprenorphine, and control solution on innate immune system function and apoptosis in healthy dogs. Six adult dogs received a 24-hour infusion of morphine, buprenorphine, or control solution (saline) in a randomized, controlled, crossover block design. Leukocyte apoptosis, phagocytosis, and oxidative burst were evaluated using flow cytometry. Lipopolysaccharide, lipoteichoic acid, and peptidoglycan-stimulated leukocyte production of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 were determined using canine specific multiplex assays. No significant treatment effects were detected among groups. These data suggest that healthy dogs could be less sensitive to the immunomodulatory effects of acute opioid administration compared with other species. Larger investigations in healthy and immunologically challenged dogs are recommended prior to application of these results in clinical patients. |
| doi_str_mv | 10.1016/j.rvsc.2015.01.010 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1668268629</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0034528815000351</els_id><sourcerecordid>1666296293</sourcerecordid><originalsourceid>FETCH-LOGICAL-c487t-f0768ea10416a6cc91d7b4bc9b19057c983fb1e781e83a71adfb04fb8dc9660c3</originalsourceid><addsrcrecordid>eNqNkc1u1DAUhS0EotPCC7BAltiwaAbbSRxHYoMqKJVadQNry7FvOp5m7OCfSnkNnriOpmXBAiFd2bL93XN1fBB6R8mWEso_7bfhIeotI7TdElqKvEAb2tasYpzTl2hDSN1ULRPiBJ3GuCeENJR2r9EJazkvB75Bv298mHfWAVbO4CHPAdzzjfHY-YTVlCDgCfK910sCXBZ_v77PwZusk_UOazUrbdNyjkGFacFq9nPy0cZz7AN2kFPwRXTC807drTJW4zG7Y7N1eAdlym4pI-_iG_RqVFOEt0_7Gfr57euPi-_V9e3l1cWX60o3okvVSDouQNHiiSuudU9NNzSD7gfak7bTvajHgUInKIhadVSZcSDNOAij--Je12fo41G3-PiVISZ5sFHDNCkHPkdJOReMC876_0ELtpIF_fAXuvc5uGJkpRpBWN-3hWJHSgcfY4BRzsEeVFgkJXINV-7lGq5cw5WEliKl6f2TdB4OYP60PKdZgM9HAMq3PVgIMmoLToOxAXSSxtt_6T8C06-4ug</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1664802995</pqid></control><display><type>article</type><title>Morphine and buprenorphine do not alter leukocyte cytokine production capacity, early apoptosis, or neutrophil phagocytic function in healthy dogs</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Monibi, Farrah A. ; Dodam, John R. ; Axiak-Bechtel, Sandra M. ; Amorim, Juliana ; Zhang, Yan ; Tsuruta, Kaoru ; Mann, F.A. ; DeClue, Amy E.</creator><creatorcontrib>Monibi, Farrah A. ; Dodam, John R. ; Axiak-Bechtel, Sandra M. ; Amorim, Juliana ; Zhang, Yan ; Tsuruta, Kaoru ; Mann, F.A. ; DeClue, Amy E.</creatorcontrib><description>•We evaluated the effects of opioids on innate immune function in healthy dogs.•Opioids do not significantly affect leukocyte stimulated cytokine production.•Opioids do not significantly affect early leukocyte apoptosis or phagocytic function.•There is a potential association between hospital stress and cytokine production.•Dogs could be less sensitive to immunomodulatory effects of acute opioid treatment.
Opioids have immunomodulatory properties in many species, but there is little information pertaining to these properties in dogs. Our objective was to compare the in vivo effects of morphine, buprenorphine, and control solution on innate immune system function and apoptosis in healthy dogs. Six adult dogs received a 24-hour infusion of morphine, buprenorphine, or control solution (saline) in a randomized, controlled, crossover block design. Leukocyte apoptosis, phagocytosis, and oxidative burst were evaluated using flow cytometry. Lipopolysaccharide, lipoteichoic acid, and peptidoglycan-stimulated leukocyte production of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 were determined using canine specific multiplex assays. No significant treatment effects were detected among groups. These data suggest that healthy dogs could be less sensitive to the immunomodulatory effects of acute opioid administration compared with other species. Larger investigations in healthy and immunologically challenged dogs are recommended prior to application of these results in clinical patients.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2015.01.010</identifier><identifier>PMID: 25660046</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Analgesics, Opioid - pharmacology ; Animals ; Apoptosis ; Apoptosis - drug effects ; Buprenorphine - pharmacology ; Colleges & universities ; Cross-Over Studies ; Cytokine ; Cytokines - metabolism ; Disease ; Dogs ; Female ; Hypotheses ; Immune system ; Immunity, Innate - drug effects ; Immunity, Innate - physiology ; Immunology ; Innate immunity ; Interleukin-10 - metabolism ; Interleukin-6 - metabolism ; Laboratories ; Leukocytes - drug effects ; Leukocytes - metabolism ; Lipopolysaccharides - pharmacology ; Male ; Metabolites ; Morphine - pharmacology ; Neutrophils - drug effects ; Neutrophils - physiology ; Opioids ; Peptidoglycan - pharmacology ; Phagocytosis - drug effects ; Phagocytosis - physiology ; Teichoic Acids - pharmacology ; Tumor Necrosis Factor-alpha - metabolism ; Veterinary medicine</subject><ispartof>Research in veterinary science, 2015-04, Vol.99, p.70-76</ispartof><rights>2015</rights><rights>Copyright © 2015. Published by Elsevier Ltd.</rights><rights>Copyright Elsevier Limited Apr 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-f0768ea10416a6cc91d7b4bc9b19057c983fb1e781e83a71adfb04fb8dc9660c3</citedby><cites>FETCH-LOGICAL-c487t-f0768ea10416a6cc91d7b4bc9b19057c983fb1e781e83a71adfb04fb8dc9660c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.rvsc.2015.01.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25660046$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Monibi, Farrah A.</creatorcontrib><creatorcontrib>Dodam, John R.</creatorcontrib><creatorcontrib>Axiak-Bechtel, Sandra M.</creatorcontrib><creatorcontrib>Amorim, Juliana</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Tsuruta, Kaoru</creatorcontrib><creatorcontrib>Mann, F.A.</creatorcontrib><creatorcontrib>DeClue, Amy E.</creatorcontrib><title>Morphine and buprenorphine do not alter leukocyte cytokine production capacity, early apoptosis, or neutrophil phagocytic function in healthy dogs</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>•We evaluated the effects of opioids on innate immune function in healthy dogs.•Opioids do not significantly affect leukocyte stimulated cytokine production.•Opioids do not significantly affect early leukocyte apoptosis or phagocytic function.•There is a potential association between hospital stress and cytokine production.•Dogs could be less sensitive to immunomodulatory effects of acute opioid treatment.
Opioids have immunomodulatory properties in many species, but there is little information pertaining to these properties in dogs. Our objective was to compare the in vivo effects of morphine, buprenorphine, and control solution on innate immune system function and apoptosis in healthy dogs. Six adult dogs received a 24-hour infusion of morphine, buprenorphine, or control solution (saline) in a randomized, controlled, crossover block design. Leukocyte apoptosis, phagocytosis, and oxidative burst were evaluated using flow cytometry. Lipopolysaccharide, lipoteichoic acid, and peptidoglycan-stimulated leukocyte production of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 were determined using canine specific multiplex assays. No significant treatment effects were detected among groups. These data suggest that healthy dogs could be less sensitive to the immunomodulatory effects of acute opioid administration compared with other species. Larger investigations in healthy and immunologically challenged dogs are recommended prior to application of these results in clinical patients.</description><subject>Analgesics, Opioid - pharmacology</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Buprenorphine - pharmacology</subject><subject>Colleges & universities</subject><subject>Cross-Over Studies</subject><subject>Cytokine</subject><subject>Cytokines - metabolism</subject><subject>Disease</subject><subject>Dogs</subject><subject>Female</subject><subject>Hypotheses</subject><subject>Immune system</subject><subject>Immunity, Innate - drug effects</subject><subject>Immunity, Innate - physiology</subject><subject>Immunology</subject><subject>Innate immunity</subject><subject>Interleukin-10 - metabolism</subject><subject>Interleukin-6 - metabolism</subject><subject>Laboratories</subject><subject>Leukocytes - drug effects</subject><subject>Leukocytes - metabolism</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Male</subject><subject>Metabolites</subject><subject>Morphine - pharmacology</subject><subject>Neutrophils - drug effects</subject><subject>Neutrophils - physiology</subject><subject>Opioids</subject><subject>Peptidoglycan - pharmacology</subject><subject>Phagocytosis - drug effects</subject><subject>Phagocytosis - physiology</subject><subject>Teichoic Acids - pharmacology</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EotPCC7BAltiwaAbbSRxHYoMqKJVadQNry7FvOp5m7OCfSnkNnriOpmXBAiFd2bL93XN1fBB6R8mWEso_7bfhIeotI7TdElqKvEAb2tasYpzTl2hDSN1ULRPiBJ3GuCeENJR2r9EJazkvB75Bv298mHfWAVbO4CHPAdzzjfHY-YTVlCDgCfK910sCXBZ_v77PwZusk_UOazUrbdNyjkGFacFq9nPy0cZz7AN2kFPwRXTC807drTJW4zG7Y7N1eAdlym4pI-_iG_RqVFOEt0_7Gfr57euPi-_V9e3l1cWX60o3okvVSDouQNHiiSuudU9NNzSD7gfak7bTvajHgUInKIhadVSZcSDNOAij--Je12fo41G3-PiVISZ5sFHDNCkHPkdJOReMC876_0ELtpIF_fAXuvc5uGJkpRpBWN-3hWJHSgcfY4BRzsEeVFgkJXINV-7lGq5cw5WEliKl6f2TdB4OYP60PKdZgM9HAMq3PVgIMmoLToOxAXSSxtt_6T8C06-4ug</recordid><startdate>20150401</startdate><enddate>20150401</enddate><creator>Monibi, Farrah A.</creator><creator>Dodam, John R.</creator><creator>Axiak-Bechtel, Sandra M.</creator><creator>Amorim, Juliana</creator><creator>Zhang, Yan</creator><creator>Tsuruta, Kaoru</creator><creator>Mann, F.A.</creator><creator>DeClue, Amy E.</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20150401</creationdate><title>Morphine and buprenorphine do not alter leukocyte cytokine production capacity, early apoptosis, or neutrophil phagocytic function in healthy dogs</title><author>Monibi, Farrah A. ; Dodam, John R. ; Axiak-Bechtel, Sandra M. ; Amorim, Juliana ; Zhang, Yan ; Tsuruta, Kaoru ; Mann, F.A. ; DeClue, Amy E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-f0768ea10416a6cc91d7b4bc9b19057c983fb1e781e83a71adfb04fb8dc9660c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analgesics, Opioid - pharmacology</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Buprenorphine - pharmacology</topic><topic>Colleges & universities</topic><topic>Cross-Over Studies</topic><topic>Cytokine</topic><topic>Cytokines - metabolism</topic><topic>Disease</topic><topic>Dogs</topic><topic>Female</topic><topic>Hypotheses</topic><topic>Immune system</topic><topic>Immunity, Innate - drug effects</topic><topic>Immunity, Innate - physiology</topic><topic>Immunology</topic><topic>Innate immunity</topic><topic>Interleukin-10 - metabolism</topic><topic>Interleukin-6 - metabolism</topic><topic>Laboratories</topic><topic>Leukocytes - drug effects</topic><topic>Leukocytes - metabolism</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Male</topic><topic>Metabolites</topic><topic>Morphine - pharmacology</topic><topic>Neutrophils - drug effects</topic><topic>Neutrophils - physiology</topic><topic>Opioids</topic><topic>Peptidoglycan - pharmacology</topic><topic>Phagocytosis - drug effects</topic><topic>Phagocytosis - physiology</topic><topic>Teichoic Acids - pharmacology</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Monibi, Farrah A.</creatorcontrib><creatorcontrib>Dodam, John R.</creatorcontrib><creatorcontrib>Axiak-Bechtel, Sandra M.</creatorcontrib><creatorcontrib>Amorim, Juliana</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Tsuruta, Kaoru</creatorcontrib><creatorcontrib>Mann, F.A.</creatorcontrib><creatorcontrib>DeClue, Amy E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Monibi, Farrah A.</au><au>Dodam, John R.</au><au>Axiak-Bechtel, Sandra M.</au><au>Amorim, Juliana</au><au>Zhang, Yan</au><au>Tsuruta, Kaoru</au><au>Mann, F.A.</au><au>DeClue, Amy E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphine and buprenorphine do not alter leukocyte cytokine production capacity, early apoptosis, or neutrophil phagocytic function in healthy dogs</atitle><jtitle>Research in veterinary science</jtitle><addtitle>Res Vet Sci</addtitle><date>2015-04-01</date><risdate>2015</risdate><volume>99</volume><spage>70</spage><epage>76</epage><pages>70-76</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>•We evaluated the effects of opioids on innate immune function in healthy dogs.•Opioids do not significantly affect leukocyte stimulated cytokine production.•Opioids do not significantly affect early leukocyte apoptosis or phagocytic function.•There is a potential association between hospital stress and cytokine production.•Dogs could be less sensitive to immunomodulatory effects of acute opioid treatment.
Opioids have immunomodulatory properties in many species, but there is little information pertaining to these properties in dogs. Our objective was to compare the in vivo effects of morphine, buprenorphine, and control solution on innate immune system function and apoptosis in healthy dogs. Six adult dogs received a 24-hour infusion of morphine, buprenorphine, or control solution (saline) in a randomized, controlled, crossover block design. Leukocyte apoptosis, phagocytosis, and oxidative burst were evaluated using flow cytometry. Lipopolysaccharide, lipoteichoic acid, and peptidoglycan-stimulated leukocyte production of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 were determined using canine specific multiplex assays. No significant treatment effects were detected among groups. These data suggest that healthy dogs could be less sensitive to the immunomodulatory effects of acute opioid administration compared with other species. Larger investigations in healthy and immunologically challenged dogs are recommended prior to application of these results in clinical patients.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>25660046</pmid><doi>10.1016/j.rvsc.2015.01.010</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0034-5288 |
| ispartof | Research in veterinary science, 2015-04, Vol.99, p.70-76 |
| issn | 0034-5288 1532-2661 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1668268629 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Analgesics, Opioid - pharmacology Animals Apoptosis Apoptosis - drug effects Buprenorphine - pharmacology Colleges & universities Cross-Over Studies Cytokine Cytokines - metabolism Disease Dogs Female Hypotheses Immune system Immunity, Innate - drug effects Immunity, Innate - physiology Immunology Innate immunity Interleukin-10 - metabolism Interleukin-6 - metabolism Laboratories Leukocytes - drug effects Leukocytes - metabolism Lipopolysaccharides - pharmacology Male Metabolites Morphine - pharmacology Neutrophils - drug effects Neutrophils - physiology Opioids Peptidoglycan - pharmacology Phagocytosis - drug effects Phagocytosis - physiology Teichoic Acids - pharmacology Tumor Necrosis Factor-alpha - metabolism Veterinary medicine |
| title | Morphine and buprenorphine do not alter leukocyte cytokine production capacity, early apoptosis, or neutrophil phagocytic function in healthy dogs |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T06%3A59%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Morphine%20and%20buprenorphine%20do%20not%20alter%20leukocyte%20cytokine%20production%20capacity,%20early%20apoptosis,%20or%20neutrophil%20phagocytic%20function%20in%20healthy%20dogs&rft.jtitle=Research%20in%20veterinary%20science&rft.au=Monibi,%20Farrah%20A.&rft.date=2015-04-01&rft.volume=99&rft.spage=70&rft.epage=76&rft.pages=70-76&rft.issn=0034-5288&rft.eissn=1532-2661&rft_id=info:doi/10.1016/j.rvsc.2015.01.010&rft_dat=%3Cproquest_cross%3E1666296293%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1664802995&rft_id=info:pmid/25660046&rft_els_id=S0034528815000351&rfr_iscdi=true |