Simultaneous measurement of cytosolic and mitochondrial calcium levels: Observations in TRPC1-silenced hepatocellular carcinoma cells

The measurement of intracellular Ca2+, cytosolic or stored in organelles, i.e., mitochondria, gave valuable data for numerous areas of research. In case of tumor cells, mitochondrial Ca2+ levels play essential roles in apoptosis along with endoplasmic reticulum (ER) Ca2+. In this study, we describe...

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Veröffentlicht in:	Journal of pharmacological and toxicological methods 2015-03, Vol.72, p.29-34
Hauptverfasser:	Selli, Cigdem, Erac, Yasemin, Tosun, Metiner
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Aorta - drug effects Aorta - physiology Apoptosis Calcium - chemistry Calcium - metabolism Carcinoma, Hepatocellular - metabolism Cell Line, Tumor Cytosol - chemistry Cytosol - metabolism Endoplasmic Reticulum - drug effects Endoplasmic Reticulum - physiology Fura-2 Gene Silencing Humans Indoles - pharmacology Liver Neoplasms - metabolism Mitochondria - chemistry Mitochondria - metabolism Phenylephrine - pharmacology Rat aorta Rats Rhod-2 Serotonin - pharmacology TRPC Cation Channels - genetics TRPC Cation Channels - metabolism Vasoconstriction - drug effects Vasoconstriction - physiology
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