Use of nucleic acids anchor system to reveal apoferritin modification by cadmium telluride nanoparticles
The aim of this study was to synthesize cadmium telluride nanoparticles (CdTe NPs) modified apoferritin, and examine if apoferritin is able to accommodate CdTe NPs. Primarily, the thermostability of horse spleen apoferritin was tested and it's unfolding at 70 °C was observed. Cadmium telluride...
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| Veröffentlicht in: | Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2015-03, Vol.3 (1), p.219-2118 |
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| creator | Kudr, Jiri Nejdl, Lukas Skalickova, Sylvie Zurek, Michal Milosavljevic, Vedran Kensova, Renata Ruttkay-Nedecky, Branislav Kopel, Pavel Hynek, David Novotna, Marie Adam, Vojtech Kizek, Rene |
| description | The aim of this study was to synthesize cadmium telluride nanoparticles (CdTe NPs) modified apoferritin, and examine if apoferritin is able to accommodate CdTe NPs. Primarily, the thermostability of horse spleen apoferritin was tested and it's unfolding at 70 °C was observed. Cadmium telluride nanoparticles (CdTe NPs) were synthesized both within apoferritin protein cage and on its surface. The thermal treatment of apoferritin with CdTe NPs resulted in the aggregation of cores, which was indicated by changes in the absorption spectra and the shape of apoferritin tryptophan fluorescence. The apoferritin modified with CdTe NPs was additionally modified with gold nanoparticles and attached to magnetic particles
via
oligonucleotide using gold affinity to thiol group. This anchor system was used to separate the construct using external magnetic field and to analyse the molecules attached to apoferritin.
The aim of this study was to synthesize cadmium telluride nanoparticles (CdTe NPs) modified apoferritin, and examine if apoferritin is able to accommodate CdTe NPs. |
| doi_str_mv | 10.1039/c4tb01336k |
| format | Article |
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via
oligonucleotide using gold affinity to thiol group. This anchor system was used to separate the construct using external magnetic field and to analyse the molecules attached to apoferritin.
The aim of this study was to synthesize cadmium telluride nanoparticles (CdTe NPs) modified apoferritin, and examine if apoferritin is able to accommodate CdTe NPs.</description><identifier>ISSN: 2050-750X</identifier><identifier>EISSN: 2050-7518</identifier><identifier>DOI: 10.1039/c4tb01336k</identifier><identifier>PMID: 32262379</identifier><language>eng</language><publisher>England</publisher><subject>Anchors ; Cadmium ; Cadmium tellurides ; Gold ; Intermetallics ; Nanoparticles ; Tellurides ; Tryptophan</subject><ispartof>Journal of materials chemistry. B, Materials for biology and medicine, 2015-03, Vol.3 (1), p.219-2118</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-eeb58caf6fc770f9f1df6135549e7c99e8f2f5519992b28cbed8dffbf9d780973</citedby><cites>FETCH-LOGICAL-c401t-eeb58caf6fc770f9f1df6135549e7c99e8f2f5519992b28cbed8dffbf9d780973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32262379$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kudr, Jiri</creatorcontrib><creatorcontrib>Nejdl, Lukas</creatorcontrib><creatorcontrib>Skalickova, Sylvie</creatorcontrib><creatorcontrib>Zurek, Michal</creatorcontrib><creatorcontrib>Milosavljevic, Vedran</creatorcontrib><creatorcontrib>Kensova, Renata</creatorcontrib><creatorcontrib>Ruttkay-Nedecky, Branislav</creatorcontrib><creatorcontrib>Kopel, Pavel</creatorcontrib><creatorcontrib>Hynek, David</creatorcontrib><creatorcontrib>Novotna, Marie</creatorcontrib><creatorcontrib>Adam, Vojtech</creatorcontrib><creatorcontrib>Kizek, Rene</creatorcontrib><title>Use of nucleic acids anchor system to reveal apoferritin modification by cadmium telluride nanoparticles</title><title>Journal of materials chemistry. B, Materials for biology and medicine</title><addtitle>J Mater Chem B</addtitle><description>The aim of this study was to synthesize cadmium telluride nanoparticles (CdTe NPs) modified apoferritin, and examine if apoferritin is able to accommodate CdTe NPs. Primarily, the thermostability of horse spleen apoferritin was tested and it's unfolding at 70 °C was observed. Cadmium telluride nanoparticles (CdTe NPs) were synthesized both within apoferritin protein cage and on its surface. The thermal treatment of apoferritin with CdTe NPs resulted in the aggregation of cores, which was indicated by changes in the absorption spectra and the shape of apoferritin tryptophan fluorescence. The apoferritin modified with CdTe NPs was additionally modified with gold nanoparticles and attached to magnetic particles
via
oligonucleotide using gold affinity to thiol group. This anchor system was used to separate the construct using external magnetic field and to analyse the molecules attached to apoferritin.
The aim of this study was to synthesize cadmium telluride nanoparticles (CdTe NPs) modified apoferritin, and examine if apoferritin is able to accommodate CdTe NPs.</description><subject>Anchors</subject><subject>Cadmium</subject><subject>Cadmium tellurides</subject><subject>Gold</subject><subject>Intermetallics</subject><subject>Nanoparticles</subject><subject>Tellurides</subject><subject>Tryptophan</subject><issn>2050-750X</issn><issn>2050-7518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkU1LHTEUhkOxVLF3474Sd0W4NR8z-VjqpbZFwc0V3A2Z5ARTZyZjMlO4_96011536sniBN6HlwMPQkeUfKOE6zNbTS2hnIuHD-iAkZosZU3V3u5P7vbRIuffpIyiQvHqE9rnjAnGpT5A97cZcPR4mG0HwWJjg8vYDPY-Jpw3eYIeTxEn-AOmw2aMHlIKUxhwH13wwZopxAG3G2yN68NcaOi6OQUHeDBDHE2aQqnOn9FHb7oMi-d9iG4vv69XP5fXNz9-rc6vl7YidFoCtLWyxgtvpSRee-q8oLyuKw3Sag3KM1_XVGvNWqZsC04571uvnVRES36Ivm57xxQfZ8hT04dsy01mgDjnhnElhazLexOlQkrNKsX1O1ChmOCEVQU93aI2xZwT-GZMoTdp01DS_FXWrKr1xT9lVwU-fu6d2x7cDv0vqABftkDKdpe-OC_5yWt5MzrPnwDTZqfV</recordid><startdate>20150314</startdate><enddate>20150314</enddate><creator>Kudr, Jiri</creator><creator>Nejdl, Lukas</creator><creator>Skalickova, Sylvie</creator><creator>Zurek, Michal</creator><creator>Milosavljevic, Vedran</creator><creator>Kensova, Renata</creator><creator>Ruttkay-Nedecky, Branislav</creator><creator>Kopel, Pavel</creator><creator>Hynek, David</creator><creator>Novotna, Marie</creator><creator>Adam, Vojtech</creator><creator>Kizek, Rene</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7QQ</scope><scope>7U5</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20150314</creationdate><title>Use of nucleic acids anchor system to reveal apoferritin modification by cadmium telluride nanoparticles</title><author>Kudr, Jiri ; Nejdl, Lukas ; Skalickova, Sylvie ; Zurek, Michal ; Milosavljevic, Vedran ; Kensova, Renata ; Ruttkay-Nedecky, Branislav ; Kopel, Pavel ; Hynek, David ; Novotna, Marie ; Adam, Vojtech ; Kizek, Rene</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-eeb58caf6fc770f9f1df6135549e7c99e8f2f5519992b28cbed8dffbf9d780973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Anchors</topic><topic>Cadmium</topic><topic>Cadmium tellurides</topic><topic>Gold</topic><topic>Intermetallics</topic><topic>Nanoparticles</topic><topic>Tellurides</topic><topic>Tryptophan</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kudr, Jiri</creatorcontrib><creatorcontrib>Nejdl, Lukas</creatorcontrib><creatorcontrib>Skalickova, Sylvie</creatorcontrib><creatorcontrib>Zurek, Michal</creatorcontrib><creatorcontrib>Milosavljevic, Vedran</creatorcontrib><creatorcontrib>Kensova, Renata</creatorcontrib><creatorcontrib>Ruttkay-Nedecky, Branislav</creatorcontrib><creatorcontrib>Kopel, Pavel</creatorcontrib><creatorcontrib>Hynek, David</creatorcontrib><creatorcontrib>Novotna, Marie</creatorcontrib><creatorcontrib>Adam, Vojtech</creatorcontrib><creatorcontrib>Kizek, Rene</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of materials chemistry. B, Materials for biology and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kudr, Jiri</au><au>Nejdl, Lukas</au><au>Skalickova, Sylvie</au><au>Zurek, Michal</au><au>Milosavljevic, Vedran</au><au>Kensova, Renata</au><au>Ruttkay-Nedecky, Branislav</au><au>Kopel, Pavel</au><au>Hynek, David</au><au>Novotna, Marie</au><au>Adam, Vojtech</au><au>Kizek, Rene</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of nucleic acids anchor system to reveal apoferritin modification by cadmium telluride nanoparticles</atitle><jtitle>Journal of materials chemistry. B, Materials for biology and medicine</jtitle><addtitle>J Mater Chem B</addtitle><date>2015-03-14</date><risdate>2015</risdate><volume>3</volume><issue>1</issue><spage>219</spage><epage>2118</epage><pages>219-2118</pages><issn>2050-750X</issn><eissn>2050-7518</eissn><abstract>The aim of this study was to synthesize cadmium telluride nanoparticles (CdTe NPs) modified apoferritin, and examine if apoferritin is able to accommodate CdTe NPs. Primarily, the thermostability of horse spleen apoferritin was tested and it's unfolding at 70 °C was observed. Cadmium telluride nanoparticles (CdTe NPs) were synthesized both within apoferritin protein cage and on its surface. The thermal treatment of apoferritin with CdTe NPs resulted in the aggregation of cores, which was indicated by changes in the absorption spectra and the shape of apoferritin tryptophan fluorescence. The apoferritin modified with CdTe NPs was additionally modified with gold nanoparticles and attached to magnetic particles
via
oligonucleotide using gold affinity to thiol group. This anchor system was used to separate the construct using external magnetic field and to analyse the molecules attached to apoferritin.
The aim of this study was to synthesize cadmium telluride nanoparticles (CdTe NPs) modified apoferritin, and examine if apoferritin is able to accommodate CdTe NPs.</abstract><cop>England</cop><pmid>32262379</pmid><doi>10.1039/c4tb01336k</doi><tpages>1</tpages></addata></record> |
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| source | Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Anchors Cadmium Cadmium tellurides Gold Intermetallics Nanoparticles Tellurides Tryptophan |
| title | Use of nucleic acids anchor system to reveal apoferritin modification by cadmium telluride nanoparticles |
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