Production of inulinase, fructosyltransferase and sucrase from fungi on low-value inulin-rich substrates and their use in generation of fructose and fructo-oligosaccharides
Owing to applications in the food and nutraceutical industries, inulinases, fructosyltransferases and sucrases have gained considerable attention in recent times. Twenty-five fungal strains were screened for production of these enzymes on three different media formulated using inulin-rich plant extr...
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Veröffentlicht in: | Antonie van Leeuwenhoek 2015-03, Vol.107 (3), p.799-811 |
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Zusammenfassung: | Owing to applications in the food and nutraceutical industries, inulinases, fructosyltransferases and sucrases have gained considerable attention in recent times. Twenty-five fungal strains were screened for production of these enzymes on three different media formulated using inulin-rich plant extracts prepared from asparagus root, dahlia tuber and dandelion root extract. Culture filtrates of the fungi were examined for hydrolytic activities. Fungi belonging to genus
Aspergillus,
A. niger
GNCC 2655 (11.3 U/ml),
A. awamori
MTCC 2879 (8.2 U/ml),
A. niger
ATCC 26011 (7.9 U/ml) secreted high titers of inulinase followed by
Penicillium
sp. NFCCI 2768 (2.6 U/ml) and
Penicillium citrinum
MTCC 1256 (1.1 U/ml). High sucrase activity was noticed in
A. niger
GNCC 2613 (113 U/ml) and
A. awamori
MTCC 2879 (107.8 U/ml). Analysis of end products of inulinase action by HPLC revealed that most of the enzymes were exo-inulinases liberating fructose exclusively from inulin. Five fungi,
P. citrinum
MTCC 1256,
Penicillium rugulosum
MTCC 3487,
Penicillium
sp. NFCCI 2768,
A. fumigatus
GNCC 1351 and
A. niger
ATCC 26011 however, produced a mixture of endo- and exo-inulinases liberating oligosaccharides (GF3 and GF2) along with fructose. High inulinase/sucrase yielding strains were evaluated for extracellular and intracellular hydrolytic and transfructosylating activities and intracellular enzyme profiles were found to be considerably different in terms of titers and end products. |
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ISSN: | 0003-6072 1572-9699 |
DOI: | 10.1007/s10482-014-0373-3 |