PLANT TRANSFORMATION VECTORS AND THEIR STABILITY IN AGROBACTERIUM TUMEFACIENS

PLANT TRANSFORMATION VECTORS AND THEIR STABILITY IN AGROBACTERIUM TUMEFACIENS

The stability of the plant transformation vectors pTS2, pEV2 and pEV8 was tested in two Agrobacterium tumefaciens strains LBA 4404 and C58C1 that differed in chromosomal background. The T-DNAs of all binary vectors contained the β-glucuronidase reporter and the neomycin phosphotransferase selectable...

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Veröffentlicht in:Journal of microbiology, biotechnology and food sciences biotechnology and food sciences, 2013-01 (Special Issue 1 - Part A), p.1559-1568
Hauptverfasser: Poloniova, Zuzana, Dubnicky, Pavol, Galova, Zdenka, Libantova, Jana, Matusikova, Ildiko, Moravcikova, Jana
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Sprache:eng
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Agrobacterium tumefaciens
Biotechnology
Cloning
E coli
Escherichia coli
Genes
Plasmids
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container_end_page 1568
container_issue Special Issue 1 - Part A
container_start_page 1559
container_title Journal of microbiology, biotechnology and food sciences
container_volume
creator Poloniova, Zuzana
Dubnicky, Pavol
Galova, Zdenka
Libantova, Jana
Matusikova, Ildiko
Moravcikova, Jana
description The stability of the plant transformation vectors pTS2, pEV2 and pEV8 was tested in two Agrobacterium tumefaciens strains LBA 4404 and C58C1 that differed in chromosomal background. The T-DNAs of all binary vectors contained the β-glucuronidase reporter and the neomycin phosphotransferase selectable marker genes. In addition, the plasmids pEV2 and pEV8 contained the Cre/loxP recombination system. The binary vectors were transformed into A. tumefaciens strains and their stability was evaluated by restriction analyses after retransformation of plasmids from A. tumefaciens into E. coli. Our results showed that plasmids pTS2 and pEV2 were stable in both bacterial strains while the pEV8 exhibited instability in A. tunmefaciens LBA 4404.
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source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
subjects Agrobacterium tumefaciens
Biotechnology
Cloning
E coli
Escherichia coli
Genes
Plasmids
title PLANT TRANSFORMATION VECTORS AND THEIR STABILITY IN AGROBACTERIUM TUMEFACIENS
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