PLANT TRANSFORMATION VECTORS AND THEIR STABILITY IN AGROBACTERIUM TUMEFACIENS

PLANT TRANSFORMATION VECTORS AND THEIR STABILITY IN AGROBACTERIUM TUMEFACIENS

The stability of the plant transformation vectors pTS2, pEV2 and pEV8 was tested in two Agrobacterium tumefaciens strains LBA 4404 and C58C1 that differed in chromosomal background. The T-DNAs of all binary vectors contained the β-glucuronidase reporter and the neomycin phosphotransferase selectable...

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Veröffentlicht in:Journal of microbiology, biotechnology and food sciences biotechnology and food sciences, 2013-01 (Special Issue 1 - Part A), p.1559-1568
Hauptverfasser: Poloniova, Zuzana, Dubnicky, Pavol, Galova, Zdenka, Libantova, Jana, Matusikova, Ildiko, Moravcikova, Jana
Format: Artikel
Sprache:eng
Schlagworte:
Agrobacterium tumefaciens
Biotechnology
Cloning
E coli
Escherichia coli
Genes
Plasmids
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Zusammenfassung:The stability of the plant transformation vectors pTS2, pEV2 and pEV8 was tested in two Agrobacterium tumefaciens strains LBA 4404 and C58C1 that differed in chromosomal background. The T-DNAs of all binary vectors contained the β-glucuronidase reporter and the neomycin phosphotransferase selectable marker genes. In addition, the plasmids pEV2 and pEV8 contained the Cre/loxP recombination system. The binary vectors were transformed into A. tumefaciens strains and their stability was evaluated by restriction analyses after retransformation of plasmids from A. tumefaciens into E. coli. Our results showed that plasmids pTS2 and pEV2 were stable in both bacterial strains while the pEV8 exhibited instability in A. tunmefaciens LBA 4404.
ISSN:1338-5178