Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses
Recombinant proteins are widely used for industrial, nutritional, and medical applications. Green microalgae have attracted considerable attention recently as a biomanufacturing platform for the production of recombinant proteins for a number of reasons. These photosynthetic eukaryotic microorganism...
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| Veröffentlicht in: | Photosynthesis research 2015-03, Vol.123 (3), p.227-239 |
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| creator | Rasala, Beth A Mayfield, Stephen P |
| description | Recombinant proteins are widely used for industrial, nutritional, and medical applications. Green microalgae have attracted considerable attention recently as a biomanufacturing platform for the production of recombinant proteins for a number of reasons. These photosynthetic eukaryotic microorganisms are safe, scalable, easy to genetically modify through transformation, mutagenesis, or breeding, and inexpensive to grow. Many microalgae species are genetically transformable, but the green alga Chlamydomonas reinhardtii is the most widely used host for recombinant protein expression. An extensive suite of molecular genetic tools has been developed for C. reinhardtii over the last 25 years, including a fully sequenced genome, well-established methods for transformation, mutagenesis and breeding, and transformation vectors for high levels of recombinant protein accumulation and secretion. Here, we review recent successes in the development of C. reinhardtii as a biomanufacturing host for recombinant proteins, including antibodies and immunotoxins, hormones, industrial enzymes, an orally-active colostral protein for gastrointestinal health, and subunit vaccines. In addition, we review the biomanufacturing potential of other green algae from the genera Dunaliella and Chlorella. |
| doi_str_mv | 10.1007/s11120-014-9994-7 |
| format | Article |
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Green microalgae have attracted considerable attention recently as a biomanufacturing platform for the production of recombinant proteins for a number of reasons. These photosynthetic eukaryotic microorganisms are safe, scalable, easy to genetically modify through transformation, mutagenesis, or breeding, and inexpensive to grow. Many microalgae species are genetically transformable, but the green alga Chlamydomonas reinhardtii is the most widely used host for recombinant protein expression. An extensive suite of molecular genetic tools has been developed for C. reinhardtii over the last 25 years, including a fully sequenced genome, well-established methods for transformation, mutagenesis and breeding, and transformation vectors for high levels of recombinant protein accumulation and secretion. Here, we review recent successes in the development of C. reinhardtii as a biomanufacturing host for recombinant proteins, including antibodies and immunotoxins, hormones, industrial enzymes, an orally-active colostral protein for gastrointestinal health, and subunit vaccines. In addition, we review the biomanufacturing potential of other green algae from the genera Dunaliella and Chlorella.</description><identifier>ISSN: 0166-8595</identifier><identifier>EISSN: 1573-5079</identifier><identifier>DOI: 10.1007/s11120-014-9994-7</identifier><identifier>PMID: 24659086</identifier><language>eng</language><publisher>Dordrecht: Springer-Verlag</publisher><subject>Algae ; Animal Feed ; Animals ; antibodies ; Antibody-toxin conjugates ; Biochemistry ; Biological products ; Biomedical and Life Sciences ; Biotechnology - methods ; breeding ; Chlamydomonas reinhardtii ; Chlamydomonas reinhardtii - genetics ; Chlamydomonas reinhardtii - metabolism ; Chlorella ; Chlorophyta - genetics ; Chlorophyta - metabolism ; Dietary Supplements ; Dunaliella ; Enzymes ; gastrointestinal system ; Genetic engineering ; Genetically modified organisms ; genome ; Genomes ; Genomics ; Health aspects ; Homeopathy ; hormones ; Humans ; Life Sciences ; Materia medica and therapeutics ; microalgae ; Microalgae - genetics ; Microalgae - metabolism ; microorganisms ; mutagenesis ; Photosynthesis ; Photosynthesis - genetics ; Photosynthesis - physiology ; Plant Genetics and Genomics ; Plant Physiology ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Proteins - therapeutic use ; Plant Sciences ; Protein Engineering - methods ; Protein expression ; protein synthesis ; Proteins ; Recombinant proteins ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Recombinant Proteins - therapeutic use ; Review ; secretion ; Studies ; subunit vaccines ; Therapeutics</subject><ispartof>Photosynthesis research, 2015-03, Vol.123 (3), p.227-239</ispartof><rights>Springer Science+Business Media Dordrecht 2014</rights><rights>COPYRIGHT 2015 Springer</rights><rights>Springer Science+Business Media Dordrecht 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c666t-ce74cfd6ff1803707fe53eb01264cabb85cdd4ffbf2ddfb3d940d3ad5d51ae1d3</citedby><cites>FETCH-LOGICAL-c666t-ce74cfd6ff1803707fe53eb01264cabb85cdd4ffbf2ddfb3d940d3ad5d51ae1d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11120-014-9994-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11120-014-9994-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24659086$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rasala, Beth A</creatorcontrib><creatorcontrib>Mayfield, Stephen P</creatorcontrib><title>Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses</title><title>Photosynthesis research</title><addtitle>Photosynth Res</addtitle><addtitle>Photosynth Res</addtitle><description>Recombinant proteins are widely used for industrial, nutritional, and medical applications. Green microalgae have attracted considerable attention recently as a biomanufacturing platform for the production of recombinant proteins for a number of reasons. These photosynthetic eukaryotic microorganisms are safe, scalable, easy to genetically modify through transformation, mutagenesis, or breeding, and inexpensive to grow. Many microalgae species are genetically transformable, but the green alga Chlamydomonas reinhardtii is the most widely used host for recombinant protein expression. An extensive suite of molecular genetic tools has been developed for C. reinhardtii over the last 25 years, including a fully sequenced genome, well-established methods for transformation, mutagenesis and breeding, and transformation vectors for high levels of recombinant protein accumulation and secretion. Here, we review recent successes in the development of C. reinhardtii as a biomanufacturing host for recombinant proteins, including antibodies and immunotoxins, hormones, industrial enzymes, an orally-active colostral protein for gastrointestinal health, and subunit vaccines. In addition, we review the biomanufacturing potential of other green algae from the genera Dunaliella and Chlorella.</description><subject>Algae</subject><subject>Animal Feed</subject><subject>Animals</subject><subject>antibodies</subject><subject>Antibody-toxin conjugates</subject><subject>Biochemistry</subject><subject>Biological products</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology - methods</subject><subject>breeding</subject><subject>Chlamydomonas reinhardtii</subject><subject>Chlamydomonas reinhardtii - genetics</subject><subject>Chlamydomonas reinhardtii - metabolism</subject><subject>Chlorella</subject><subject>Chlorophyta - genetics</subject><subject>Chlorophyta - metabolism</subject><subject>Dietary Supplements</subject><subject>Dunaliella</subject><subject>Enzymes</subject><subject>gastrointestinal system</subject><subject>Genetic engineering</subject><subject>Genetically modified organisms</subject><subject>genome</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Health aspects</subject><subject>Homeopathy</subject><subject>hormones</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Materia medica and therapeutics</subject><subject>microalgae</subject><subject>Microalgae - genetics</subject><subject>Microalgae - metabolism</subject><subject>microorganisms</subject><subject>mutagenesis</subject><subject>Photosynthesis</subject><subject>Photosynthesis - genetics</subject><subject>Photosynthesis - physiology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Physiology</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Proteins - therapeutic use</subject><subject>Plant Sciences</subject><subject>Protein Engineering - methods</subject><subject>Protein expression</subject><subject>protein synthesis</subject><subject>Proteins</subject><subject>Recombinant proteins</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Recombinant Proteins - therapeutic use</subject><subject>Review</subject><subject>secretion</subject><subject>Studies</subject><subject>subunit vaccines</subject><subject>Therapeutics</subject><issn>0166-8595</issn><issn>1573-5079</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kl1rFTEQhhdR7Gn1B3ijAW8quDXZTbK7eFWKH4WCYu11yCaTbcpucpoPsHf-dLOc-nFEJBfJTJ53mEneqnpG8AnBuHsTCSENrjGh9TAMtO4eVBvCurZmuBseVhtMOK97NrCD6jDGG4xxz0n7uDpoKGdDCTbV98_XPvl459I1JKvQaP0iXTZSpRysm5B1aAoADsl5kvAWbYPXWSXrHfIGBVB-Ga2TLq03CayLyPhQZDrHFKycXyOXy2FVrIF0Gi2grZIzyhHik-qRkXOEp_f7UXX1_t3Xs4_1xacP52enF7XinKdaQUeV0dwY0uO2w50B1sKIScOpkuPYM6U1NWY0jdZmbPVAsW6lZpoRCUS3R9Xxrm5p8zZDTGKxUcE8Swc-R1Geqm8Y7mlf0Jd_oTc-h9L9SjHGe9K03W9qkjMI64xPQaq1qDiluGNNT3peqJN_UGVpWKzyDowt-T3Bqz1BYRJ8S5PMMYrzyy_7LNmxKvgYAxixDXaR4U4QLFaHiJ1DRHGIWB0i1raf3w-Xx_IPvxQ_LVGAZgfE7WoACH9M_5-qL3YiI72QU7BRXF02mDCMG9wSStsfOhTQrg</recordid><startdate>20150301</startdate><enddate>20150301</enddate><creator>Rasala, Beth A</creator><creator>Mayfield, Stephen P</creator><general>Springer-Verlag</general><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QP</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>F1W</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope></search><sort><creationdate>20150301</creationdate><title>Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses</title><author>Rasala, Beth A ; Mayfield, Stephen P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c666t-ce74cfd6ff1803707fe53eb01264cabb85cdd4ffbf2ddfb3d940d3ad5d51ae1d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Algae</topic><topic>Animal Feed</topic><topic>Animals</topic><topic>antibodies</topic><topic>Antibody-toxin conjugates</topic><topic>Biochemistry</topic><topic>Biological products</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology - methods</topic><topic>breeding</topic><topic>Chlamydomonas reinhardtii</topic><topic>Chlamydomonas reinhardtii - genetics</topic><topic>Chlamydomonas reinhardtii - metabolism</topic><topic>Chlorella</topic><topic>Chlorophyta - genetics</topic><topic>Chlorophyta - metabolism</topic><topic>Dietary Supplements</topic><topic>Dunaliella</topic><topic>Enzymes</topic><topic>gastrointestinal system</topic><topic>Genetic engineering</topic><topic>Genetically modified organisms</topic><topic>genome</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Health aspects</topic><topic>Homeopathy</topic><topic>hormones</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Materia medica and therapeutics</topic><topic>microalgae</topic><topic>Microalgae - genetics</topic><topic>Microalgae - metabolism</topic><topic>microorganisms</topic><topic>mutagenesis</topic><topic>Photosynthesis</topic><topic>Photosynthesis - genetics</topic><topic>Photosynthesis - physiology</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Physiology</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Proteins - therapeutic use</topic><topic>Plant Sciences</topic><topic>Protein Engineering - methods</topic><topic>Protein expression</topic><topic>protein synthesis</topic><topic>Proteins</topic><topic>Recombinant proteins</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>Recombinant Proteins - therapeutic use</topic><topic>Review</topic><topic>secretion</topic><topic>Studies</topic><topic>subunit vaccines</topic><topic>Therapeutics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rasala, Beth A</creatorcontrib><creatorcontrib>Mayfield, Stephen P</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Photosynthesis research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rasala, Beth A</au><au>Mayfield, Stephen P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses</atitle><jtitle>Photosynthesis research</jtitle><stitle>Photosynth Res</stitle><addtitle>Photosynth Res</addtitle><date>2015-03-01</date><risdate>2015</risdate><volume>123</volume><issue>3</issue><spage>227</spage><epage>239</epage><pages>227-239</pages><issn>0166-8595</issn><eissn>1573-5079</eissn><abstract>Recombinant proteins are widely used for industrial, nutritional, and medical applications. Green microalgae have attracted considerable attention recently as a biomanufacturing platform for the production of recombinant proteins for a number of reasons. These photosynthetic eukaryotic microorganisms are safe, scalable, easy to genetically modify through transformation, mutagenesis, or breeding, and inexpensive to grow. Many microalgae species are genetically transformable, but the green alga Chlamydomonas reinhardtii is the most widely used host for recombinant protein expression. An extensive suite of molecular genetic tools has been developed for C. reinhardtii over the last 25 years, including a fully sequenced genome, well-established methods for transformation, mutagenesis and breeding, and transformation vectors for high levels of recombinant protein accumulation and secretion. Here, we review recent successes in the development of C. reinhardtii as a biomanufacturing host for recombinant proteins, including antibodies and immunotoxins, hormones, industrial enzymes, an orally-active colostral protein for gastrointestinal health, and subunit vaccines. In addition, we review the biomanufacturing potential of other green algae from the genera Dunaliella and Chlorella.</abstract><cop>Dordrecht</cop><pub>Springer-Verlag</pub><pmid>24659086</pmid><doi>10.1007/s11120-014-9994-7</doi><tpages>13</tpages></addata></record> |
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| ispartof | Photosynthesis research, 2015-03, Vol.123 (3), p.227-239 |
| issn | 0166-8595 1573-5079 |
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| subjects | Algae Animal Feed Animals antibodies Antibody-toxin conjugates Biochemistry Biological products Biomedical and Life Sciences Biotechnology - methods breeding Chlamydomonas reinhardtii Chlamydomonas reinhardtii - genetics Chlamydomonas reinhardtii - metabolism Chlorella Chlorophyta - genetics Chlorophyta - metabolism Dietary Supplements Dunaliella Enzymes gastrointestinal system Genetic engineering Genetically modified organisms genome Genomes Genomics Health aspects Homeopathy hormones Humans Life Sciences Materia medica and therapeutics microalgae Microalgae - genetics Microalgae - metabolism microorganisms mutagenesis Photosynthesis Photosynthesis - genetics Photosynthesis - physiology Plant Genetics and Genomics Plant Physiology Plant Proteins - genetics Plant Proteins - metabolism Plant Proteins - therapeutic use Plant Sciences Protein Engineering - methods Protein expression protein synthesis Proteins Recombinant proteins Recombinant Proteins - genetics Recombinant Proteins - metabolism Recombinant Proteins - therapeutic use Review secretion Studies subunit vaccines Therapeutics |
| title | Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses |
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