Identification and genetic mapping of the putative Thinopyrum intermedium-derived dominant powdery mildew resistance gene PmL962 on wheat chromosome arm 2BS
Key message Powdery resistance putatively derived from Thinopyrum intermedium in the wheat line L962 is controlled by a single dominant gene designated PmL962 and mapped to chromosome arm 2BS. Powdery mildew, caused by Blumeria graminis f. sp. tritici ( Bgt ), is a destructive disease affecting the...
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| Veröffentlicht in: | Theoretical and applied genetics 2015-03, Vol.128 (3), p.517-528 |
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| creator | Shen, X. K. Ma, L. X. Zhong, S. F. Liu, N. Zhang, M. Chen, W. Q. Zhou, Y. L. Li, H. J. Chang, Z. J. Li, X. Bai, G. H. Zhang, H. Y. Tan, F. Q. Ren, Z. L. Luo, P. G. |
| description | Key message
Powdery resistance putatively derived from
Thinopyrum intermedium
in the wheat line L962 is controlled by a single dominant gene designated
PmL962
and mapped to chromosome arm 2BS.
Powdery mildew, caused by
Blumeria graminis
f. sp.
tritici
(
Bgt
), is a destructive disease affecting the production of wheat (
Triticum aestivum
). Powdery mildew resistance was putatively transferred from
Thinopyrum intermedium
to the common wheat line L962, which conferred resistance to multiple Chinese
Bgt
isolates. Genetic analysis of the powdery mildew response was conducted by crossing the resistant line L962 with the susceptible line L983. Disease assessments of the F
1
, F
2
, and F
2:3
populations from the cross L983/L962 indicated that resistance was controlled by a single dominant gene. A total of 373 F
2:3
lines and 781 pairs of genomic simple sequence repeat (SSR) primers were employed to determine the chromosomal location of the resistance gene. The gene was linked to four publicly available and recently developed wheat genomic SSR markers and seven EST-STS markers. The resistance gene was mapped to chromosome arm 2BS based on the locations of the linked markers. Pedigree, molecular marker and resistance response data indicated that the powdery mildew resistance gene in L962 is novel. It was temporarily designated
PmL962
. It is flanked by
Xwmc314
and
BE443737
at genetic distances of 2.09 and 3.74 cM, respectively, and located in a 20.77 cM interval that is co-linear with a 269.4 kb genomic region on chromosome 5 in
Brachypodium distachyon
and a 223.5 kb genomic region on rice (
Oryza sativa
) chromosome 4. The markers that are closely linked to this gene have potential applications in marker-assisted breeding. |
| doi_str_mv | 10.1007/s00122-014-2449-x |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1668250068</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A423836056</galeid><sourcerecordid>A423836056</sourcerecordid><originalsourceid>FETCH-LOGICAL-c576t-71e4ff1eea0908e0866ee7023c70eedaedc78bbacc7aaf90e3b0ffa51623f7093</originalsourceid><addsrcrecordid>eNqNkk1vEzEQhlcIREPhB3BBlrjAYcvYu-vdPbYVH5EigWg5W453nLiK7cX20uS_8GNxSPkIAgn5YGnmeWc0M29RPKVwRgHaVxGAMlYCrUtW1325vVfMaF2xkrGa3S9mADWUTduwk-JRjDcAwBqoHhYnrGka3ld0VnydD-iS0UbJZLwj0g1khQ6TUcTKcTRuRbwmaY1knFJmviC5Xhvnx12YLDEuYbA4mMmWA4acHcjgrXHSJTL62xzbEWs2A96SgNHEJJ3C7x3IB7voOSO56e0aZSJqHbz10VskMljCLq4eFw-03ER8cvefFp_evL6-fFcu3r-dX54vStW0PJUtxVpriiihhw6h4xyxBVapFhAHiYNqu-VSKtVKqXvAaglay4ZyVukW-uq0eHGoOwb_ecKYhDVR4WYjHfopCsp5lzcHvPsPtGkr1vFmjz7_A73xU3B5kEy1fb5Tz_kvaiU3KIzTPgWp9kXFec2qruLQ7Kmzv1D5DWiN8g61yfEjwcsjQWYSbtNKTjGK-dXHY5YeWBV8jAG1GIOxMuwEBbE3mjgYTWSjib3RxDZrnt0NNy3z_X8qfjgrA-wAxJxyKwy_Tf_Pqt8APJreng</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1679432966</pqid></control><display><type>article</type><title>Identification and genetic mapping of the putative Thinopyrum intermedium-derived dominant powdery mildew resistance gene PmL962 on wheat chromosome arm 2BS</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Shen, X. K. ; Ma, L. X. ; Zhong, S. F. ; Liu, N. ; Zhang, M. ; Chen, W. Q. ; Zhou, Y. L. ; Li, H. J. ; Chang, Z. J. ; Li, X. ; Bai, G. H. ; Zhang, H. Y. ; Tan, F. Q. ; Ren, Z. L. ; Luo, P. G.</creator><creatorcontrib>Shen, X. K. ; Ma, L. X. ; Zhong, S. F. ; Liu, N. ; Zhang, M. ; Chen, W. Q. ; Zhou, Y. L. ; Li, H. J. ; Chang, Z. J. ; Li, X. ; Bai, G. H. ; Zhang, H. Y. ; Tan, F. Q. ; Ren, Z. L. ; Luo, P. G.</creatorcontrib><description>Key message
Powdery resistance putatively derived from
Thinopyrum intermedium
in the wheat line L962 is controlled by a single dominant gene designated
PmL962
and mapped to chromosome arm 2BS.
Powdery mildew, caused by
Blumeria graminis
f. sp.
tritici
(
Bgt
), is a destructive disease affecting the production of wheat (
Triticum aestivum
). Powdery mildew resistance was putatively transferred from
Thinopyrum intermedium
to the common wheat line L962, which conferred resistance to multiple Chinese
Bgt
isolates. Genetic analysis of the powdery mildew response was conducted by crossing the resistant line L962 with the susceptible line L983. Disease assessments of the F
1
, F
2
, and F
2:3
populations from the cross L983/L962 indicated that resistance was controlled by a single dominant gene. A total of 373 F
2:3
lines and 781 pairs of genomic simple sequence repeat (SSR) primers were employed to determine the chromosomal location of the resistance gene. The gene was linked to four publicly available and recently developed wheat genomic SSR markers and seven EST-STS markers. The resistance gene was mapped to chromosome arm 2BS based on the locations of the linked markers. Pedigree, molecular marker and resistance response data indicated that the powdery mildew resistance gene in L962 is novel. It was temporarily designated
PmL962
. It is flanked by
Xwmc314
and
BE443737
at genetic distances of 2.09 and 3.74 cM, respectively, and located in a 20.77 cM interval that is co-linear with a 269.4 kb genomic region on chromosome 5 in
Brachypodium distachyon
and a 223.5 kb genomic region on rice (
Oryza sativa
) chromosome 4. The markers that are closely linked to this gene have potential applications in marker-assisted breeding.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s00122-014-2449-x</identifier><identifier>PMID: 25556931</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Agriculture ; Ascomycota ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Blumeria graminis ; Brachypodium distachyon ; Breeding ; Chromosome Mapping ; Chromosomes ; Chromosomes, Plant ; Crop diseases ; Crosses, Genetic ; Cultivars ; Disease Resistance - genetics ; Diseases and pests ; Expressed Sequence Tags ; Genes ; Genes, Dominant ; Genes, Plant ; Genetic aspects ; Genetic Linkage ; Genetic Markers ; Genetics ; Genomes ; Health aspects ; Inheritance Patterns ; Life Sciences ; Methods ; Microsatellite Repeats ; Mildew ; Original Paper ; Oryza sativa ; Plant Biochemistry ; Plant Breeding/Biotechnology ; Plant diseases ; Plant Diseases - genetics ; Plant Diseases - microbiology ; Plant Genetics and Genomics ; Plant immunology ; Plant-pathogen relationships ; Poaceae - genetics ; Thinopyrum intermedium ; Triticum - genetics ; Triticum - microbiology ; Triticum aestivum ; Wheat</subject><ispartof>Theoretical and applied genetics, 2015-03, Vol.128 (3), p.517-528</ispartof><rights>Springer-Verlag Berlin Heidelberg 2014</rights><rights>COPYRIGHT 2015 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c576t-71e4ff1eea0908e0866ee7023c70eedaedc78bbacc7aaf90e3b0ffa51623f7093</citedby><cites>FETCH-LOGICAL-c576t-71e4ff1eea0908e0866ee7023c70eedaedc78bbacc7aaf90e3b0ffa51623f7093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00122-014-2449-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00122-014-2449-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25556931$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shen, X. K.</creatorcontrib><creatorcontrib>Ma, L. X.</creatorcontrib><creatorcontrib>Zhong, S. F.</creatorcontrib><creatorcontrib>Liu, N.</creatorcontrib><creatorcontrib>Zhang, M.</creatorcontrib><creatorcontrib>Chen, W. Q.</creatorcontrib><creatorcontrib>Zhou, Y. L.</creatorcontrib><creatorcontrib>Li, H. J.</creatorcontrib><creatorcontrib>Chang, Z. J.</creatorcontrib><creatorcontrib>Li, X.</creatorcontrib><creatorcontrib>Bai, G. H.</creatorcontrib><creatorcontrib>Zhang, H. Y.</creatorcontrib><creatorcontrib>Tan, F. Q.</creatorcontrib><creatorcontrib>Ren, Z. L.</creatorcontrib><creatorcontrib>Luo, P. G.</creatorcontrib><title>Identification and genetic mapping of the putative Thinopyrum intermedium-derived dominant powdery mildew resistance gene PmL962 on wheat chromosome arm 2BS</title><title>Theoretical and applied genetics</title><addtitle>Theor Appl Genet</addtitle><addtitle>Theor Appl Genet</addtitle><description>Key message
Powdery resistance putatively derived from
Thinopyrum intermedium
in the wheat line L962 is controlled by a single dominant gene designated
PmL962
and mapped to chromosome arm 2BS.
Powdery mildew, caused by
Blumeria graminis
f. sp.
tritici
(
Bgt
), is a destructive disease affecting the production of wheat (
Triticum aestivum
). Powdery mildew resistance was putatively transferred from
Thinopyrum intermedium
to the common wheat line L962, which conferred resistance to multiple Chinese
Bgt
isolates. Genetic analysis of the powdery mildew response was conducted by crossing the resistant line L962 with the susceptible line L983. Disease assessments of the F
1
, F
2
, and F
2:3
populations from the cross L983/L962 indicated that resistance was controlled by a single dominant gene. A total of 373 F
2:3
lines and 781 pairs of genomic simple sequence repeat (SSR) primers were employed to determine the chromosomal location of the resistance gene. The gene was linked to four publicly available and recently developed wheat genomic SSR markers and seven EST-STS markers. The resistance gene was mapped to chromosome arm 2BS based on the locations of the linked markers. Pedigree, molecular marker and resistance response data indicated that the powdery mildew resistance gene in L962 is novel. It was temporarily designated
PmL962
. It is flanked by
Xwmc314
and
BE443737
at genetic distances of 2.09 and 3.74 cM, respectively, and located in a 20.77 cM interval that is co-linear with a 269.4 kb genomic region on chromosome 5 in
Brachypodium distachyon
and a 223.5 kb genomic region on rice (
Oryza sativa
) chromosome 4. The markers that are closely linked to this gene have potential applications in marker-assisted breeding.</description><subject>Agriculture</subject><subject>Ascomycota</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Blumeria graminis</subject><subject>Brachypodium distachyon</subject><subject>Breeding</subject><subject>Chromosome Mapping</subject><subject>Chromosomes</subject><subject>Chromosomes, Plant</subject><subject>Crop diseases</subject><subject>Crosses, Genetic</subject><subject>Cultivars</subject><subject>Disease Resistance - genetics</subject><subject>Diseases and pests</subject><subject>Expressed Sequence Tags</subject><subject>Genes</subject><subject>Genes, Dominant</subject><subject>Genes, Plant</subject><subject>Genetic aspects</subject><subject>Genetic Linkage</subject><subject>Genetic Markers</subject><subject>Genetics</subject><subject>Genomes</subject><subject>Health aspects</subject><subject>Inheritance Patterns</subject><subject>Life Sciences</subject><subject>Methods</subject><subject>Microsatellite Repeats</subject><subject>Mildew</subject><subject>Original Paper</subject><subject>Oryza sativa</subject><subject>Plant Biochemistry</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant diseases</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant immunology</subject><subject>Plant-pathogen relationships</subject><subject>Poaceae - genetics</subject><subject>Thinopyrum intermedium</subject><subject>Triticum - genetics</subject><subject>Triticum - microbiology</subject><subject>Triticum aestivum</subject><subject>Wheat</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkk1vEzEQhlcIREPhB3BBlrjAYcvYu-vdPbYVH5EigWg5W453nLiK7cX20uS_8GNxSPkIAgn5YGnmeWc0M29RPKVwRgHaVxGAMlYCrUtW1325vVfMaF2xkrGa3S9mADWUTduwk-JRjDcAwBqoHhYnrGka3ld0VnydD-iS0UbJZLwj0g1khQ6TUcTKcTRuRbwmaY1knFJmviC5Xhvnx12YLDEuYbA4mMmWA4acHcjgrXHSJTL62xzbEWs2A96SgNHEJJ3C7x3IB7voOSO56e0aZSJqHbz10VskMljCLq4eFw-03ER8cvefFp_evL6-fFcu3r-dX54vStW0PJUtxVpriiihhw6h4xyxBVapFhAHiYNqu-VSKtVKqXvAaglay4ZyVukW-uq0eHGoOwb_ecKYhDVR4WYjHfopCsp5lzcHvPsPtGkr1vFmjz7_A73xU3B5kEy1fb5Tz_kvaiU3KIzTPgWp9kXFec2qruLQ7Kmzv1D5DWiN8g61yfEjwcsjQWYSbtNKTjGK-dXHY5YeWBV8jAG1GIOxMuwEBbE3mjgYTWSjib3RxDZrnt0NNy3z_X8qfjgrA-wAxJxyKwy_Tf_Pqt8APJreng</recordid><startdate>20150301</startdate><enddate>20150301</enddate><creator>Shen, X. K.</creator><creator>Ma, L. X.</creator><creator>Zhong, S. F.</creator><creator>Liu, N.</creator><creator>Zhang, M.</creator><creator>Chen, W. Q.</creator><creator>Zhou, Y. L.</creator><creator>Li, H. J.</creator><creator>Chang, Z. J.</creator><creator>Li, X.</creator><creator>Bai, G. H.</creator><creator>Zhang, H. Y.</creator><creator>Tan, F. Q.</creator><creator>Ren, Z. L.</creator><creator>Luo, P. G.</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>M7N</scope></search><sort><creationdate>20150301</creationdate><title>Identification and genetic mapping of the putative Thinopyrum intermedium-derived dominant powdery mildew resistance gene PmL962 on wheat chromosome arm 2BS</title><author>Shen, X. K. ; Ma, L. X. ; Zhong, S. F. ; Liu, N. ; Zhang, M. ; Chen, W. Q. ; Zhou, Y. L. ; Li, H. J. ; Chang, Z. J. ; Li, X. ; Bai, G. H. ; Zhang, H. Y. ; Tan, F. Q. ; Ren, Z. L. ; Luo, P. G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c576t-71e4ff1eea0908e0866ee7023c70eedaedc78bbacc7aaf90e3b0ffa51623f7093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Agriculture</topic><topic>Ascomycota</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Blumeria graminis</topic><topic>Brachypodium distachyon</topic><topic>Breeding</topic><topic>Chromosome Mapping</topic><topic>Chromosomes</topic><topic>Chromosomes, Plant</topic><topic>Crop diseases</topic><topic>Crosses, Genetic</topic><topic>Cultivars</topic><topic>Disease Resistance - genetics</topic><topic>Diseases and pests</topic><topic>Expressed Sequence Tags</topic><topic>Genes</topic><topic>Genes, Dominant</topic><topic>Genes, Plant</topic><topic>Genetic aspects</topic><topic>Genetic Linkage</topic><topic>Genetic Markers</topic><topic>Genetics</topic><topic>Genomes</topic><topic>Health aspects</topic><topic>Inheritance Patterns</topic><topic>Life Sciences</topic><topic>Methods</topic><topic>Microsatellite Repeats</topic><topic>Mildew</topic><topic>Original Paper</topic><topic>Oryza sativa</topic><topic>Plant Biochemistry</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant diseases</topic><topic>Plant Diseases - genetics</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Genetics and Genomics</topic><topic>Plant immunology</topic><topic>Plant-pathogen relationships</topic><topic>Poaceae - genetics</topic><topic>Thinopyrum intermedium</topic><topic>Triticum - genetics</topic><topic>Triticum - microbiology</topic><topic>Triticum aestivum</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shen, X. K.</creatorcontrib><creatorcontrib>Ma, L. X.</creatorcontrib><creatorcontrib>Zhong, S. F.</creatorcontrib><creatorcontrib>Liu, N.</creatorcontrib><creatorcontrib>Zhang, M.</creatorcontrib><creatorcontrib>Chen, W. Q.</creatorcontrib><creatorcontrib>Zhou, Y. L.</creatorcontrib><creatorcontrib>Li, H. J.</creatorcontrib><creatorcontrib>Chang, Z. J.</creatorcontrib><creatorcontrib>Li, X.</creatorcontrib><creatorcontrib>Bai, G. H.</creatorcontrib><creatorcontrib>Zhang, H. Y.</creatorcontrib><creatorcontrib>Tan, F. Q.</creatorcontrib><creatorcontrib>Ren, Z. L.</creatorcontrib><creatorcontrib>Luo, P. G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Theoretical and applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shen, X. K.</au><au>Ma, L. X.</au><au>Zhong, S. F.</au><au>Liu, N.</au><au>Zhang, M.</au><au>Chen, W. Q.</au><au>Zhou, Y. L.</au><au>Li, H. J.</au><au>Chang, Z. J.</au><au>Li, X.</au><au>Bai, G. H.</au><au>Zhang, H. Y.</au><au>Tan, F. Q.</au><au>Ren, Z. L.</au><au>Luo, P. G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and genetic mapping of the putative Thinopyrum intermedium-derived dominant powdery mildew resistance gene PmL962 on wheat chromosome arm 2BS</atitle><jtitle>Theoretical and applied genetics</jtitle><stitle>Theor Appl Genet</stitle><addtitle>Theor Appl Genet</addtitle><date>2015-03-01</date><risdate>2015</risdate><volume>128</volume><issue>3</issue><spage>517</spage><epage>528</epage><pages>517-528</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><abstract>Key message
Powdery resistance putatively derived from
Thinopyrum intermedium
in the wheat line L962 is controlled by a single dominant gene designated
PmL962
and mapped to chromosome arm 2BS.
Powdery mildew, caused by
Blumeria graminis
f. sp.
tritici
(
Bgt
), is a destructive disease affecting the production of wheat (
Triticum aestivum
). Powdery mildew resistance was putatively transferred from
Thinopyrum intermedium
to the common wheat line L962, which conferred resistance to multiple Chinese
Bgt
isolates. Genetic analysis of the powdery mildew response was conducted by crossing the resistant line L962 with the susceptible line L983. Disease assessments of the F
1
, F
2
, and F
2:3
populations from the cross L983/L962 indicated that resistance was controlled by a single dominant gene. A total of 373 F
2:3
lines and 781 pairs of genomic simple sequence repeat (SSR) primers were employed to determine the chromosomal location of the resistance gene. The gene was linked to four publicly available and recently developed wheat genomic SSR markers and seven EST-STS markers. The resistance gene was mapped to chromosome arm 2BS based on the locations of the linked markers. Pedigree, molecular marker and resistance response data indicated that the powdery mildew resistance gene in L962 is novel. It was temporarily designated
PmL962
. It is flanked by
Xwmc314
and
BE443737
at genetic distances of 2.09 and 3.74 cM, respectively, and located in a 20.77 cM interval that is co-linear with a 269.4 kb genomic region on chromosome 5 in
Brachypodium distachyon
and a 223.5 kb genomic region on rice (
Oryza sativa
) chromosome 4. The markers that are closely linked to this gene have potential applications in marker-assisted breeding.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>25556931</pmid><doi>10.1007/s00122-014-2449-x</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0040-5752 |
| ispartof | Theoretical and applied genetics, 2015-03, Vol.128 (3), p.517-528 |
| issn | 0040-5752 1432-2242 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1668250068 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Agriculture Ascomycota Biochemistry Biomedical and Life Sciences Biotechnology Blumeria graminis Brachypodium distachyon Breeding Chromosome Mapping Chromosomes Chromosomes, Plant Crop diseases Crosses, Genetic Cultivars Disease Resistance - genetics Diseases and pests Expressed Sequence Tags Genes Genes, Dominant Genes, Plant Genetic aspects Genetic Linkage Genetic Markers Genetics Genomes Health aspects Inheritance Patterns Life Sciences Methods Microsatellite Repeats Mildew Original Paper Oryza sativa Plant Biochemistry Plant Breeding/Biotechnology Plant diseases Plant Diseases - genetics Plant Diseases - microbiology Plant Genetics and Genomics Plant immunology Plant-pathogen relationships Poaceae - genetics Thinopyrum intermedium Triticum - genetics Triticum - microbiology Triticum aestivum Wheat |
| title | Identification and genetic mapping of the putative Thinopyrum intermedium-derived dominant powdery mildew resistance gene PmL962 on wheat chromosome arm 2BS |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T09%3A14%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20and%20genetic%20mapping%20of%20the%20putative%20Thinopyrum%20intermedium-derived%20dominant%20powdery%20mildew%20resistance%20gene%20PmL962%20on%20wheat%20chromosome%20arm%202BS&rft.jtitle=Theoretical%20and%20applied%20genetics&rft.au=Shen,%20X.%20K.&rft.date=2015-03-01&rft.volume=128&rft.issue=3&rft.spage=517&rft.epage=528&rft.pages=517-528&rft.issn=0040-5752&rft.eissn=1432-2242&rft_id=info:doi/10.1007/s00122-014-2449-x&rft_dat=%3Cgale_proqu%3EA423836056%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1679432966&rft_id=info:pmid/25556931&rft_galeid=A423836056&rfr_iscdi=true |