Extremely Low-Frequency Magnetic Fields Induce Developmental Toxicity and Apoptosis in Zebrafish (Danio rerio) Embryos
Extremely low-frequency (ELF) magnetic field (MF), as a widespread ecological factor, has an influence on all living beings. In the present study, biological effects of ELF-MF on the development of zebrafish ( Danio rerio ) embryos were investigated. Fertilized embryos were divided into seven groups...
Gespeichert in:
| Veröffentlicht in: | Biological trace element research 2014-12, Vol.162 (1-3), p.324-332 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 332 |
|---|---|
| container_issue | 1-3 |
| container_start_page | 324 |
| container_title | Biological trace element research |
| container_volume | 162 |
| creator | Li, Ying Liu, Xingfa Liu, Keran Miao, Wei Zhou, Cheng Li, Yang Wu, Hongjuan |
| description | Extremely low-frequency (ELF) magnetic field (MF), as a widespread ecological factor, has an influence on all living beings. In the present study, biological effects of ELF-MF on the development of zebrafish (
Danio rerio
) embryos were investigated. Fertilized embryos were divided into seven groups as control, sham, and five experimental groups. Embryos of experimental groups were continuously exposed to 50-Hz sinusoidal MF with intensities of 30, 100, 200, 400, and 800 μT for 96 h. The sham group was treated as the experimental groups, but without any ELF-MF exposure. The control group was not subjected to anything. The results showed that ELF-MF exposure caused delayed hatching and decreased heart rate at the early developmental stages of zebrafish embryos, whereas no significant differences in embryo mortality and abnormality were observed. Moreover, acridine orange staining assays showed notable signals of apoptosis mainly in the ventral fin and spinal column. The transcription of apoptosis-related genes (
caspase-3
,
caspase-9
) was significantly upregulated in ELF-MF-exposed embryos. In conclusion, the overall results demonstrated that ELF-MF exposure has detrimental effects on the embryonic development of zebrafish by affecting the hatching, decreasing the heart rate, and inducing apoptosis, although such effects were not mortal threat. The results also indicate that zebrafish embryos can serve as a reliable model to investigate the biological effect of ELF-MF. |
| doi_str_mv | 10.1007/s12011-014-0130-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1668247036</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1668247036</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-1000dcf1f333ebf915d7b9806052803711527d5e2e1939f9acb8b19c0752e3cd3</originalsourceid><addsrcrecordid>eNp1kcFu1DAURS0EotPCB7BBlti0i4CfHcfxsmpnoNJU3ZQNGytxXoqrxA52Upq_x9UUhJBYWF743Ov3dAh5B-wjMKY-JeAMoGBQ5iNYIV-QDUipC6Y4e0k2DCpRlLouj8hxSveMgeJavCZHXPIqR-sNedg-zhFHHFa6Dz-LXcQfC3q70uvmzuPsLN05HLpEr3y3WKSX-IBDmEb0czPQ2_DorJtX2viOnk9hmkNyiTpPv2Ebm96l7_T0svEu0IjRhTO6Hdu4hvSGvOqbIeHb5_uEfN1tby--FPubz1cX5_vClkrORd6SdbaHXgiBba9BdqrVNauY5DUTCkBy1UnkCFroXje2rVvQlinJUdhOnJDTQ-8UQ14szWZ0yeIwNB7DkgxUVc1LxUSV0Q__oPdhiT5PlylegxK6hkzBgbIxpBSxN1N0YxNXA8w8STEHKSZLMU9SjMyZ98_NSzti9yfx20IG-AFI-cnfYfzr6_-2_gKx6ZZz</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1628173981</pqid></control><display><type>article</type><title>Extremely Low-Frequency Magnetic Fields Induce Developmental Toxicity and Apoptosis in Zebrafish (Danio rerio) Embryos</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Li, Ying ; Liu, Xingfa ; Liu, Keran ; Miao, Wei ; Zhou, Cheng ; Li, Yang ; Wu, Hongjuan</creator><creatorcontrib>Li, Ying ; Liu, Xingfa ; Liu, Keran ; Miao, Wei ; Zhou, Cheng ; Li, Yang ; Wu, Hongjuan</creatorcontrib><description>Extremely low-frequency (ELF) magnetic field (MF), as a widespread ecological factor, has an influence on all living beings. In the present study, biological effects of ELF-MF on the development of zebrafish (
Danio rerio
) embryos were investigated. Fertilized embryos were divided into seven groups as control, sham, and five experimental groups. Embryos of experimental groups were continuously exposed to 50-Hz sinusoidal MF with intensities of 30, 100, 200, 400, and 800 μT for 96 h. The sham group was treated as the experimental groups, but without any ELF-MF exposure. The control group was not subjected to anything. The results showed that ELF-MF exposure caused delayed hatching and decreased heart rate at the early developmental stages of zebrafish embryos, whereas no significant differences in embryo mortality and abnormality were observed. Moreover, acridine orange staining assays showed notable signals of apoptosis mainly in the ventral fin and spinal column. The transcription of apoptosis-related genes (
caspase-3
,
caspase-9
) was significantly upregulated in ELF-MF-exposed embryos. In conclusion, the overall results demonstrated that ELF-MF exposure has detrimental effects on the embryonic development of zebrafish by affecting the hatching, decreasing the heart rate, and inducing apoptosis, although such effects were not mortal threat. The results also indicate that zebrafish embryos can serve as a reliable model to investigate the biological effect of ELF-MF.</description><identifier>ISSN: 0163-4984</identifier><identifier>EISSN: 1559-0720</identifier><identifier>DOI: 10.1007/s12011-014-0130-5</identifier><identifier>PMID: 25262018</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Animals ; Apoptosis ; Apoptosis - radiation effects ; Biochemistry ; Biological effects ; Biomedical and Life Sciences ; Biotechnology ; Danio rerio ; Developmental stages ; Embryo, Nonmammalian - radiation effects ; Embryonic growth stage ; Embryos ; Fish ; Hatching ; Heart rate ; Life Sciences ; Magnetic fields ; Magnetic Fields - adverse effects ; Nutrition ; Oncology ; Toxicity ; Zebrafish</subject><ispartof>Biological trace element research, 2014-12, Vol.162 (1-3), p.324-332</ispartof><rights>Springer Science+Business Media New York 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-1000dcf1f333ebf915d7b9806052803711527d5e2e1939f9acb8b19c0752e3cd3</citedby><cites>FETCH-LOGICAL-c475t-1000dcf1f333ebf915d7b9806052803711527d5e2e1939f9acb8b19c0752e3cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12011-014-0130-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12011-014-0130-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25262018$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Ying</creatorcontrib><creatorcontrib>Liu, Xingfa</creatorcontrib><creatorcontrib>Liu, Keran</creatorcontrib><creatorcontrib>Miao, Wei</creatorcontrib><creatorcontrib>Zhou, Cheng</creatorcontrib><creatorcontrib>Li, Yang</creatorcontrib><creatorcontrib>Wu, Hongjuan</creatorcontrib><title>Extremely Low-Frequency Magnetic Fields Induce Developmental Toxicity and Apoptosis in Zebrafish (Danio rerio) Embryos</title><title>Biological trace element research</title><addtitle>Biol Trace Elem Res</addtitle><addtitle>Biol Trace Elem Res</addtitle><description>Extremely low-frequency (ELF) magnetic field (MF), as a widespread ecological factor, has an influence on all living beings. In the present study, biological effects of ELF-MF on the development of zebrafish (
Danio rerio
) embryos were investigated. Fertilized embryos were divided into seven groups as control, sham, and five experimental groups. Embryos of experimental groups were continuously exposed to 50-Hz sinusoidal MF with intensities of 30, 100, 200, 400, and 800 μT for 96 h. The sham group was treated as the experimental groups, but without any ELF-MF exposure. The control group was not subjected to anything. The results showed that ELF-MF exposure caused delayed hatching and decreased heart rate at the early developmental stages of zebrafish embryos, whereas no significant differences in embryo mortality and abnormality were observed. Moreover, acridine orange staining assays showed notable signals of apoptosis mainly in the ventral fin and spinal column. The transcription of apoptosis-related genes (
caspase-3
,
caspase-9
) was significantly upregulated in ELF-MF-exposed embryos. In conclusion, the overall results demonstrated that ELF-MF exposure has detrimental effects on the embryonic development of zebrafish by affecting the hatching, decreasing the heart rate, and inducing apoptosis, although such effects were not mortal threat. The results also indicate that zebrafish embryos can serve as a reliable model to investigate the biological effect of ELF-MF.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - radiation effects</subject><subject>Biochemistry</subject><subject>Biological effects</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Danio rerio</subject><subject>Developmental stages</subject><subject>Embryo, Nonmammalian - radiation effects</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>Fish</subject><subject>Hatching</subject><subject>Heart rate</subject><subject>Life Sciences</subject><subject>Magnetic fields</subject><subject>Magnetic Fields - adverse effects</subject><subject>Nutrition</subject><subject>Oncology</subject><subject>Toxicity</subject><subject>Zebrafish</subject><issn>0163-4984</issn><issn>1559-0720</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kcFu1DAURS0EotPCB7BBlti0i4CfHcfxsmpnoNJU3ZQNGytxXoqrxA52Upq_x9UUhJBYWF743Ov3dAh5B-wjMKY-JeAMoGBQ5iNYIV-QDUipC6Y4e0k2DCpRlLouj8hxSveMgeJavCZHXPIqR-sNedg-zhFHHFa6Dz-LXcQfC3q70uvmzuPsLN05HLpEr3y3WKSX-IBDmEb0czPQ2_DorJtX2viOnk9hmkNyiTpPv2Ebm96l7_T0svEu0IjRhTO6Hdu4hvSGvOqbIeHb5_uEfN1tby--FPubz1cX5_vClkrORd6SdbaHXgiBba9BdqrVNauY5DUTCkBy1UnkCFroXje2rVvQlinJUdhOnJDTQ-8UQ14szWZ0yeIwNB7DkgxUVc1LxUSV0Q__oPdhiT5PlylegxK6hkzBgbIxpBSxN1N0YxNXA8w8STEHKSZLMU9SjMyZ98_NSzti9yfx20IG-AFI-cnfYfzr6_-2_gKx6ZZz</recordid><startdate>20141201</startdate><enddate>20141201</enddate><creator>Li, Ying</creator><creator>Liu, Xingfa</creator><creator>Liu, Keran</creator><creator>Miao, Wei</creator><creator>Zhou, Cheng</creator><creator>Li, Yang</creator><creator>Wu, Hongjuan</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QH</scope><scope>7QP</scope><scope>7TN</scope><scope>7U7</scope><scope>7UA</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H97</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>20141201</creationdate><title>Extremely Low-Frequency Magnetic Fields Induce Developmental Toxicity and Apoptosis in Zebrafish (Danio rerio) Embryos</title><author>Li, Ying ; Liu, Xingfa ; Liu, Keran ; Miao, Wei ; Zhou, Cheng ; Li, Yang ; Wu, Hongjuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-1000dcf1f333ebf915d7b9806052803711527d5e2e1939f9acb8b19c0752e3cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - radiation effects</topic><topic>Biochemistry</topic><topic>Biological effects</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Danio rerio</topic><topic>Developmental stages</topic><topic>Embryo, Nonmammalian - radiation effects</topic><topic>Embryonic growth stage</topic><topic>Embryos</topic><topic>Fish</topic><topic>Hatching</topic><topic>Heart rate</topic><topic>Life Sciences</topic><topic>Magnetic fields</topic><topic>Magnetic Fields - adverse effects</topic><topic>Nutrition</topic><topic>Oncology</topic><topic>Toxicity</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Ying</creatorcontrib><creatorcontrib>Liu, Xingfa</creatorcontrib><creatorcontrib>Liu, Keran</creatorcontrib><creatorcontrib>Miao, Wei</creatorcontrib><creatorcontrib>Zhou, Cheng</creatorcontrib><creatorcontrib>Li, Yang</creatorcontrib><creatorcontrib>Wu, Hongjuan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database (ProQuest)</collection><collection>Biological Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Biological trace element research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Ying</au><au>Liu, Xingfa</au><au>Liu, Keran</au><au>Miao, Wei</au><au>Zhou, Cheng</au><au>Li, Yang</au><au>Wu, Hongjuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extremely Low-Frequency Magnetic Fields Induce Developmental Toxicity and Apoptosis in Zebrafish (Danio rerio) Embryos</atitle><jtitle>Biological trace element research</jtitle><stitle>Biol Trace Elem Res</stitle><addtitle>Biol Trace Elem Res</addtitle><date>2014-12-01</date><risdate>2014</risdate><volume>162</volume><issue>1-3</issue><spage>324</spage><epage>332</epage><pages>324-332</pages><issn>0163-4984</issn><eissn>1559-0720</eissn><abstract>Extremely low-frequency (ELF) magnetic field (MF), as a widespread ecological factor, has an influence on all living beings. In the present study, biological effects of ELF-MF on the development of zebrafish (
Danio rerio
) embryos were investigated. Fertilized embryos were divided into seven groups as control, sham, and five experimental groups. Embryos of experimental groups were continuously exposed to 50-Hz sinusoidal MF with intensities of 30, 100, 200, 400, and 800 μT for 96 h. The sham group was treated as the experimental groups, but without any ELF-MF exposure. The control group was not subjected to anything. The results showed that ELF-MF exposure caused delayed hatching and decreased heart rate at the early developmental stages of zebrafish embryos, whereas no significant differences in embryo mortality and abnormality were observed. Moreover, acridine orange staining assays showed notable signals of apoptosis mainly in the ventral fin and spinal column. The transcription of apoptosis-related genes (
caspase-3
,
caspase-9
) was significantly upregulated in ELF-MF-exposed embryos. In conclusion, the overall results demonstrated that ELF-MF exposure has detrimental effects on the embryonic development of zebrafish by affecting the hatching, decreasing the heart rate, and inducing apoptosis, although such effects were not mortal threat. The results also indicate that zebrafish embryos can serve as a reliable model to investigate the biological effect of ELF-MF.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>25262018</pmid><doi>10.1007/s12011-014-0130-5</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0163-4984 |
| ispartof | Biological trace element research, 2014-12, Vol.162 (1-3), p.324-332 |
| issn | 0163-4984 1559-0720 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1668247036 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Apoptosis Apoptosis - radiation effects Biochemistry Biological effects Biomedical and Life Sciences Biotechnology Danio rerio Developmental stages Embryo, Nonmammalian - radiation effects Embryonic growth stage Embryos Fish Hatching Heart rate Life Sciences Magnetic fields Magnetic Fields - adverse effects Nutrition Oncology Toxicity Zebrafish |
| title | Extremely Low-Frequency Magnetic Fields Induce Developmental Toxicity and Apoptosis in Zebrafish (Danio rerio) Embryos |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T14%3A32%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Extremely%20Low-Frequency%20Magnetic%20Fields%20Induce%20Developmental%20Toxicity%20and%20Apoptosis%20in%20Zebrafish%20(Danio%20rerio)%20Embryos&rft.jtitle=Biological%20trace%20element%20research&rft.au=Li,%20Ying&rft.date=2014-12-01&rft.volume=162&rft.issue=1-3&rft.spage=324&rft.epage=332&rft.pages=324-332&rft.issn=0163-4984&rft.eissn=1559-0720&rft_id=info:doi/10.1007/s12011-014-0130-5&rft_dat=%3Cproquest_cross%3E1668247036%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1628173981&rft_id=info:pmid/25262018&rfr_iscdi=true |