Human Milk Bactericidal Properties: Effect of Lyophilization and Relation to Maternal Factors and Milk Components
OBJECTIVE:Lyophilization appears to be a viable method for storing human milk, assuring no microbiological contamination and preserving its health benefits and antibacterial properties. The aim of the study is to evaluate and compare the effects of different storage methods (lyophilization and freez...
Gespeichert in:
| Veröffentlicht in: | Journal of pediatric gastroenterology and nutrition 2015-04, Vol.60 (4), p.527-532 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 532 |
|---|---|
| container_issue | 4 |
| container_start_page | 527 |
| container_title | Journal of pediatric gastroenterology and nutrition |
| container_volume | 60 |
| creator | Salcedo, Jaime Gormaz, Maria López-Mendoza, Maria C. Nogarotto, Elisabetta Silvestre, Dolores |
| description | OBJECTIVE:Lyophilization appears to be a viable method for storing human milk, assuring no microbiological contamination and preserving its health benefits and antibacterial properties. The aim of the study is to evaluate and compare the effects of different storage methods (lyophilization and freezing at −20°C and −80°C) and maternal factors (gestational length or time postpartum) upon the microbiological contents and bactericidal activity of human milk. The possible relation between bactericidal activity and the content of certain nutrients and functional components is also investigated.
METHODS:Microbiological content, bactericidal activity, sialic acid, and ganglioside contents, as well as protein, fat, and lactose concentrations were assessed in 125 human milk samples from 65 healthy donors in the Human Milk Bank of La Fe (Valencia, Spain).
RESULTS:Lyophilization and storage at −80°C significantly reduced the content of mesophilic aerobic microorganisms and Staphylococcus epidermidis when compared with storage at −20°C. Bactericidal activity was not significantly modified by lyophilization when compared with freezing at either −20°C or −80°C. Bactericidal activity was not correlated with fat, protein, or lactose content, but was significantly correlated to ganglioside content. The bactericidal activity was significantly greater (P |
| doi_str_mv | 10.1097/MPG.0000000000000641 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1668238410</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1668238410</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3751-95e58695647fc3941f6fed96cf4fd49570e552e1b71fc28768b15168ac4e07b53</originalsourceid><addsrcrecordid>eNp9kMtKxDAYRoMoOl7eQCRLN9WkzaV1p4M3mEERXZdM-oeJpk1NWkSf3uioiAuzCYHzncBBaJ-SI0oqeTy_vTwiv49gdA1NKC9ExkpC19GE5FJmOaViC23H-JgYyTjZRFs5Z0TwvJig56uxVR2eW_eEz5QeIFhtG-XwbfA9hMFCPMHnxoAesDd49ur7pXX2TQ3Wd1h1Db4Dt3oMHs9VEnRpfZFUPsRP4NM99W3vO-iGuIs2jHIR9r7uHfRwcX4_vcpmN5fX09NZpgvJaVZx4KWouGDS6KJi1AgDTSW0YaZhFZcEOM-BLiQ1Oi-lKBeUU1EqzYDIBS920OHK2wf_PEIc6tZGDc6pDvwYaypEmRcloyShbIXq4GMMYOo-2FaF15qS-iN2nWLXf2On2cHXD-OiheZn9F03AeUKePEuhYlPbnyBUC9BuWH5v_sdQLKLKg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1668238410</pqid></control><display><type>article</type><title>Human Milk Bactericidal Properties: Effect of Lyophilization and Relation to Maternal Factors and Milk Components</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Journals@Ovid Complete</source><creator>Salcedo, Jaime ; Gormaz, Maria ; López-Mendoza, Maria C. ; Nogarotto, Elisabetta ; Silvestre, Dolores</creator><creatorcontrib>Salcedo, Jaime ; Gormaz, Maria ; López-Mendoza, Maria C. ; Nogarotto, Elisabetta ; Silvestre, Dolores</creatorcontrib><description>OBJECTIVE:Lyophilization appears to be a viable method for storing human milk, assuring no microbiological contamination and preserving its health benefits and antibacterial properties. The aim of the study is to evaluate and compare the effects of different storage methods (lyophilization and freezing at −20°C and −80°C) and maternal factors (gestational length or time postpartum) upon the microbiological contents and bactericidal activity of human milk. The possible relation between bactericidal activity and the content of certain nutrients and functional components is also investigated.
METHODS:Microbiological content, bactericidal activity, sialic acid, and ganglioside contents, as well as protein, fat, and lactose concentrations were assessed in 125 human milk samples from 65 healthy donors in the Human Milk Bank of La Fe (Valencia, Spain).
RESULTS:Lyophilization and storage at −80°C significantly reduced the content of mesophilic aerobic microorganisms and Staphylococcus epidermidis when compared with storage at −20°C. Bactericidal activity was not significantly modified by lyophilization when compared with freezing at either −20°C or −80°C. Bactericidal activity was not correlated with fat, protein, or lactose content, but was significantly correlated to ganglioside content. The bactericidal activity was significantly greater (P < 0.05) in mature milk and in milk from women with term delivery than in milk from early lactation (days 1–7 postpartum) and milk from women with preterm delivery, respectively.
CONCLUSIONS:Lyophilization and storage at −80°C of human milk yields similar results and are superior to storage at −20C with regard to microbial and bactericidal capacities, being a feasible alternative for human milk banks.</description><identifier>ISSN: 0277-2116</identifier><identifier>EISSN: 1536-4801</identifier><identifier>DOI: 10.1097/MPG.0000000000000641</identifier><identifier>PMID: 25406523</identifier><language>eng</language><publisher>United States: by European Society for Pediatric Gastroenterology, Hepatology, and Nutrition and North American Society for Pediatric Gastroenterology</publisher><subject>Anti-Bacterial Agents - analysis ; Cold Temperature ; Female ; Food Storage - methods ; Freeze Drying ; Freezing ; Gangliosides - analysis ; Gestational Age ; Humans ; Infant, Newborn ; Lactation ; Milk Banks ; Milk, Human - chemistry ; Milk, Human - microbiology ; Postpartum Period ; Premature Birth ; Refrigeration ; Spain ; Staphylococcus epidermidis ; Term Birth</subject><ispartof>Journal of pediatric gastroenterology and nutrition, 2015-04, Vol.60 (4), p.527-532</ispartof><rights>2015 by European Society for Pediatric Gastroenterology, Hepatology, and Nutrition and North American Society for Pediatric Gastroenterology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3751-95e58695647fc3941f6fed96cf4fd49570e552e1b71fc28768b15168ac4e07b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25406523$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Salcedo, Jaime</creatorcontrib><creatorcontrib>Gormaz, Maria</creatorcontrib><creatorcontrib>López-Mendoza, Maria C.</creatorcontrib><creatorcontrib>Nogarotto, Elisabetta</creatorcontrib><creatorcontrib>Silvestre, Dolores</creatorcontrib><title>Human Milk Bactericidal Properties: Effect of Lyophilization and Relation to Maternal Factors and Milk Components</title><title>Journal of pediatric gastroenterology and nutrition</title><addtitle>J Pediatr Gastroenterol Nutr</addtitle><description>OBJECTIVE:Lyophilization appears to be a viable method for storing human milk, assuring no microbiological contamination and preserving its health benefits and antibacterial properties. The aim of the study is to evaluate and compare the effects of different storage methods (lyophilization and freezing at −20°C and −80°C) and maternal factors (gestational length or time postpartum) upon the microbiological contents and bactericidal activity of human milk. The possible relation between bactericidal activity and the content of certain nutrients and functional components is also investigated.
METHODS:Microbiological content, bactericidal activity, sialic acid, and ganglioside contents, as well as protein, fat, and lactose concentrations were assessed in 125 human milk samples from 65 healthy donors in the Human Milk Bank of La Fe (Valencia, Spain).
RESULTS:Lyophilization and storage at −80°C significantly reduced the content of mesophilic aerobic microorganisms and Staphylococcus epidermidis when compared with storage at −20°C. Bactericidal activity was not significantly modified by lyophilization when compared with freezing at either −20°C or −80°C. Bactericidal activity was not correlated with fat, protein, or lactose content, but was significantly correlated to ganglioside content. The bactericidal activity was significantly greater (P < 0.05) in mature milk and in milk from women with term delivery than in milk from early lactation (days 1–7 postpartum) and milk from women with preterm delivery, respectively.
CONCLUSIONS:Lyophilization and storage at −80°C of human milk yields similar results and are superior to storage at −20C with regard to microbial and bactericidal capacities, being a feasible alternative for human milk banks.</description><subject>Anti-Bacterial Agents - analysis</subject><subject>Cold Temperature</subject><subject>Female</subject><subject>Food Storage - methods</subject><subject>Freeze Drying</subject><subject>Freezing</subject><subject>Gangliosides - analysis</subject><subject>Gestational Age</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Lactation</subject><subject>Milk Banks</subject><subject>Milk, Human - chemistry</subject><subject>Milk, Human - microbiology</subject><subject>Postpartum Period</subject><subject>Premature Birth</subject><subject>Refrigeration</subject><subject>Spain</subject><subject>Staphylococcus epidermidis</subject><subject>Term Birth</subject><issn>0277-2116</issn><issn>1536-4801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKxDAYRoMoOl7eQCRLN9WkzaV1p4M3mEERXZdM-oeJpk1NWkSf3uioiAuzCYHzncBBaJ-SI0oqeTy_vTwiv49gdA1NKC9ExkpC19GE5FJmOaViC23H-JgYyTjZRFs5Z0TwvJig56uxVR2eW_eEz5QeIFhtG-XwbfA9hMFCPMHnxoAesDd49ur7pXX2TQ3Wd1h1Db4Dt3oMHs9VEnRpfZFUPsRP4NM99W3vO-iGuIs2jHIR9r7uHfRwcX4_vcpmN5fX09NZpgvJaVZx4KWouGDS6KJi1AgDTSW0YaZhFZcEOM-BLiQ1Oi-lKBeUU1EqzYDIBS920OHK2wf_PEIc6tZGDc6pDvwYaypEmRcloyShbIXq4GMMYOo-2FaF15qS-iN2nWLXf2On2cHXD-OiheZn9F03AeUKePEuhYlPbnyBUC9BuWH5v_sdQLKLKg</recordid><startdate>201504</startdate><enddate>201504</enddate><creator>Salcedo, Jaime</creator><creator>Gormaz, Maria</creator><creator>López-Mendoza, Maria C.</creator><creator>Nogarotto, Elisabetta</creator><creator>Silvestre, Dolores</creator><general>by European Society for Pediatric Gastroenterology, Hepatology, and Nutrition and North American Society for Pediatric Gastroenterology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201504</creationdate><title>Human Milk Bactericidal Properties: Effect of Lyophilization and Relation to Maternal Factors and Milk Components</title><author>Salcedo, Jaime ; Gormaz, Maria ; López-Mendoza, Maria C. ; Nogarotto, Elisabetta ; Silvestre, Dolores</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3751-95e58695647fc3941f6fed96cf4fd49570e552e1b71fc28768b15168ac4e07b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Anti-Bacterial Agents - analysis</topic><topic>Cold Temperature</topic><topic>Female</topic><topic>Food Storage - methods</topic><topic>Freeze Drying</topic><topic>Freezing</topic><topic>Gangliosides - analysis</topic><topic>Gestational Age</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Lactation</topic><topic>Milk Banks</topic><topic>Milk, Human - chemistry</topic><topic>Milk, Human - microbiology</topic><topic>Postpartum Period</topic><topic>Premature Birth</topic><topic>Refrigeration</topic><topic>Spain</topic><topic>Staphylococcus epidermidis</topic><topic>Term Birth</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Salcedo, Jaime</creatorcontrib><creatorcontrib>Gormaz, Maria</creatorcontrib><creatorcontrib>López-Mendoza, Maria C.</creatorcontrib><creatorcontrib>Nogarotto, Elisabetta</creatorcontrib><creatorcontrib>Silvestre, Dolores</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pediatric gastroenterology and nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salcedo, Jaime</au><au>Gormaz, Maria</au><au>López-Mendoza, Maria C.</au><au>Nogarotto, Elisabetta</au><au>Silvestre, Dolores</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human Milk Bactericidal Properties: Effect of Lyophilization and Relation to Maternal Factors and Milk Components</atitle><jtitle>Journal of pediatric gastroenterology and nutrition</jtitle><addtitle>J Pediatr Gastroenterol Nutr</addtitle><date>2015-04</date><risdate>2015</risdate><volume>60</volume><issue>4</issue><spage>527</spage><epage>532</epage><pages>527-532</pages><issn>0277-2116</issn><eissn>1536-4801</eissn><abstract>OBJECTIVE:Lyophilization appears to be a viable method for storing human milk, assuring no microbiological contamination and preserving its health benefits and antibacterial properties. The aim of the study is to evaluate and compare the effects of different storage methods (lyophilization and freezing at −20°C and −80°C) and maternal factors (gestational length or time postpartum) upon the microbiological contents and bactericidal activity of human milk. The possible relation between bactericidal activity and the content of certain nutrients and functional components is also investigated.
METHODS:Microbiological content, bactericidal activity, sialic acid, and ganglioside contents, as well as protein, fat, and lactose concentrations were assessed in 125 human milk samples from 65 healthy donors in the Human Milk Bank of La Fe (Valencia, Spain).
RESULTS:Lyophilization and storage at −80°C significantly reduced the content of mesophilic aerobic microorganisms and Staphylococcus epidermidis when compared with storage at −20°C. Bactericidal activity was not significantly modified by lyophilization when compared with freezing at either −20°C or −80°C. Bactericidal activity was not correlated with fat, protein, or lactose content, but was significantly correlated to ganglioside content. The bactericidal activity was significantly greater (P < 0.05) in mature milk and in milk from women with term delivery than in milk from early lactation (days 1–7 postpartum) and milk from women with preterm delivery, respectively.
CONCLUSIONS:Lyophilization and storage at −80°C of human milk yields similar results and are superior to storage at −20C with regard to microbial and bactericidal capacities, being a feasible alternative for human milk banks.</abstract><cop>United States</cop><pub>by European Society for Pediatric Gastroenterology, Hepatology, and Nutrition and North American Society for Pediatric Gastroenterology</pub><pmid>25406523</pmid><doi>10.1097/MPG.0000000000000641</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0277-2116 |
| ispartof | Journal of pediatric gastroenterology and nutrition, 2015-04, Vol.60 (4), p.527-532 |
| issn | 0277-2116 1536-4801 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1668238410 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Journals@Ovid Complete |
| subjects | Anti-Bacterial Agents - analysis Cold Temperature Female Food Storage - methods Freeze Drying Freezing Gangliosides - analysis Gestational Age Humans Infant, Newborn Lactation Milk Banks Milk, Human - chemistry Milk, Human - microbiology Postpartum Period Premature Birth Refrigeration Spain Staphylococcus epidermidis Term Birth |
| title | Human Milk Bactericidal Properties: Effect of Lyophilization and Relation to Maternal Factors and Milk Components |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T02%3A20%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Human%20Milk%20Bactericidal%20Properties:%20Effect%20of%20Lyophilization%20and%20Relation%20to%20Maternal%20Factors%20and%20Milk%20Components&rft.jtitle=Journal%20of%20pediatric%20gastroenterology%20and%20nutrition&rft.au=Salcedo,%20Jaime&rft.date=2015-04&rft.volume=60&rft.issue=4&rft.spage=527&rft.epage=532&rft.pages=527-532&rft.issn=0277-2116&rft.eissn=1536-4801&rft_id=info:doi/10.1097/MPG.0000000000000641&rft_dat=%3Cproquest_cross%3E1668238410%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1668238410&rft_id=info:pmid/25406523&rfr_iscdi=true |