A G-protein β-subunit is essential for Dictyostelium development
Recent studies have demonstrated that G-protein-linked signal transduction pathways play a significant role in the developmental program of the simple eukaryotic organism Dictyostelium. We have reported previously the isolation of a G-protein beta-subunit and present here a more complete analysis of...
Gespeichert in:
| Veröffentlicht in: | Genes & development 1993-06, Vol.7 (6), p.986-995 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 995 |
|---|---|
| container_issue | 6 |
| container_start_page | 986 |
| container_title | Genes & development |
| container_volume | 7 |
| creator | LILLY, P LIJUN WU WELKER, D. L DEVREOTES, P. N |
| description | Recent studies have demonstrated that G-protein-linked signal transduction pathways play a significant role in the developmental program of the simple eukaryotic organism Dictyostelium. We have reported previously the isolation of a G-protein beta-subunit and present here a more complete analysis of this gene. Low-stringency Southern blots and RFLP mapping studies suggest that the beta-subunit is a unique gene found on linkage group II. Its deduced amino acid sequence of 347 residues is approximately 60% identical to those of the human, Drosophila, and Caenorhabditis elegans beta-subunits. The carboxy-terminal 300 residues are about 70% identical; the amino-terminal 50 residues are quite divergent, containing only 10 identities. At all stages of growth and development, a single 1.9-kb beta-subunit mRNA is present at a high level, and a specific antibody detects a single 37-kD protein. We propose that G-protein heterotrimers are formed when this beta-subunit couples with each of the eight distinct G-protein alpha-subunits that are transiently expressed during development. Targeted disruption of the beta-subunit gene had no effect on the viability of haploid cells, but resulted in the inability of cells to aggregate. |
| doi_str_mv | 10.1101/gad.7.6.986 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_16673404</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16673404</sourcerecordid><originalsourceid>FETCH-LOGICAL-c381t-33f770ebaf5c301f7eb1e68dbbd96022c5f8c558480977031d783df15bda60333</originalsourceid><addsrcrecordid>eNo9kMtKAzEYhYMotVZXroVZiBuZ8U8zuS1L1SoU3Og6ZHKRyFzqZEboa_kgPpORDq7O4nwcDh9ClxgKjAHfvWtb8IIVUrAjNMe0lDktOT9GcxASckmYPEVnMX4AAAPGZmgmQEpC6BytVtkm3_Xd4EKb_XzncazGNgxZiJmL0bVD0HXmuz67D2bYd3FwdRibzLovV3e7JgHn6MTrOrqLKRfo7fHhdf2Ub182z-vVNjdE4CEnxHMOrtKeGgLYc1dhx4StKisZLJeGemEoFWW6lkCCLRfEekwrqxkQQhbo5rCb3n6OLg6qCdG4utat68aoMGOclFAm8PYAmr6LsXde7frQ6H6vMKg_YSoJU1wxlYQl-mqaHavG2X92MpT666nX0eja97o1If5jJReY4pL8AkVEc2M</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16673404</pqid></control><display><type>article</type><title>A G-protein β-subunit is essential for Dictyostelium development</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>LILLY, P ; LIJUN WU ; WELKER, D. L ; DEVREOTES, P. N</creator><creatorcontrib>LILLY, P ; LIJUN WU ; WELKER, D. L ; DEVREOTES, P. N</creatorcontrib><description>Recent studies have demonstrated that G-protein-linked signal transduction pathways play a significant role in the developmental program of the simple eukaryotic organism Dictyostelium. We have reported previously the isolation of a G-protein beta-subunit and present here a more complete analysis of this gene. Low-stringency Southern blots and RFLP mapping studies suggest that the beta-subunit is a unique gene found on linkage group II. Its deduced amino acid sequence of 347 residues is approximately 60% identical to those of the human, Drosophila, and Caenorhabditis elegans beta-subunits. The carboxy-terminal 300 residues are about 70% identical; the amino-terminal 50 residues are quite divergent, containing only 10 identities. At all stages of growth and development, a single 1.9-kb beta-subunit mRNA is present at a high level, and a specific antibody detects a single 37-kD protein. We propose that G-protein heterotrimers are formed when this beta-subunit couples with each of the eight distinct G-protein alpha-subunits that are transiently expressed during development. Targeted disruption of the beta-subunit gene had no effect on the viability of haploid cells, but resulted in the inability of cells to aggregate.</description><identifier>ISSN: 0890-9369</identifier><identifier>EISSN: 1549-5477</identifier><identifier>DOI: 10.1101/gad.7.6.986</identifier><identifier>PMID: 8099335</identifier><identifier>CODEN: GEDEEP</identifier><language>eng</language><publisher>Cold Spring Harbor, NY: Cold Spring Harbor Laboratory</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Cell Line ; Classical genetics, quantitative genetics, hybrids ; Dictyostelium - genetics ; Dictyostelium - growth & development ; Dictyostelium discoideum ; DNA, Antisense - genetics ; DNA, Fungal - analysis ; DNA, Fungal - chemistry ; DNA, Protozoan - analysis ; DNA, Protozoan - chemistry ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genetics of eukaryotes. Biological and molecular evolution ; GTP-Binding Proteins - chemistry ; GTP-Binding Proteins - genetics ; GTP-Binding Proteins - physiology ; Molecular Sequence Data ; Mutagenesis ; Oligonucleotides - chemistry ; Peptide Fragments - chemistry ; Peptide Fragments - genetics ; Peptide Fragments - physiology ; Polymorphism, Restriction Fragment Length ; Protozoa ; Sequence Alignment ; Sequence Homology, Amino Acid ; Signal Transduction</subject><ispartof>Genes & development, 1993-06, Vol.7 (6), p.986-995</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-33f770ebaf5c301f7eb1e68dbbd96022c5f8c558480977031d783df15bda60333</citedby><cites>FETCH-LOGICAL-c381t-33f770ebaf5c301f7eb1e68dbbd96022c5f8c558480977031d783df15bda60333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4781514$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8099335$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LILLY, P</creatorcontrib><creatorcontrib>LIJUN WU</creatorcontrib><creatorcontrib>WELKER, D. L</creatorcontrib><creatorcontrib>DEVREOTES, P. N</creatorcontrib><title>A G-protein β-subunit is essential for Dictyostelium development</title><title>Genes & development</title><addtitle>Genes Dev</addtitle><description>Recent studies have demonstrated that G-protein-linked signal transduction pathways play a significant role in the developmental program of the simple eukaryotic organism Dictyostelium. We have reported previously the isolation of a G-protein beta-subunit and present here a more complete analysis of this gene. Low-stringency Southern blots and RFLP mapping studies suggest that the beta-subunit is a unique gene found on linkage group II. Its deduced amino acid sequence of 347 residues is approximately 60% identical to those of the human, Drosophila, and Caenorhabditis elegans beta-subunits. The carboxy-terminal 300 residues are about 70% identical; the amino-terminal 50 residues are quite divergent, containing only 10 identities. At all stages of growth and development, a single 1.9-kb beta-subunit mRNA is present at a high level, and a specific antibody detects a single 37-kD protein. We propose that G-protein heterotrimers are formed when this beta-subunit couples with each of the eight distinct G-protein alpha-subunits that are transiently expressed during development. Targeted disruption of the beta-subunit gene had no effect on the viability of haploid cells, but resulted in the inability of cells to aggregate.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Dictyostelium - genetics</subject><subject>Dictyostelium - growth & development</subject><subject>Dictyostelium discoideum</subject><subject>DNA, Antisense - genetics</subject><subject>DNA, Fungal - analysis</subject><subject>DNA, Fungal - chemistry</subject><subject>DNA, Protozoan - analysis</subject><subject>DNA, Protozoan - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>GTP-Binding Proteins - chemistry</subject><subject>GTP-Binding Proteins - genetics</subject><subject>GTP-Binding Proteins - physiology</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis</subject><subject>Oligonucleotides - chemistry</subject><subject>Peptide Fragments - chemistry</subject><subject>Peptide Fragments - genetics</subject><subject>Peptide Fragments - physiology</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Protozoa</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Signal Transduction</subject><issn>0890-9369</issn><issn>1549-5477</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtKAzEYhYMotVZXroVZiBuZ8U8zuS1L1SoU3Og6ZHKRyFzqZEboa_kgPpORDq7O4nwcDh9ClxgKjAHfvWtb8IIVUrAjNMe0lDktOT9GcxASckmYPEVnMX4AAAPGZmgmQEpC6BytVtkm3_Xd4EKb_XzncazGNgxZiJmL0bVD0HXmuz67D2bYd3FwdRibzLovV3e7JgHn6MTrOrqLKRfo7fHhdf2Ub182z-vVNjdE4CEnxHMOrtKeGgLYc1dhx4StKisZLJeGemEoFWW6lkCCLRfEekwrqxkQQhbo5rCb3n6OLg6qCdG4utat68aoMGOclFAm8PYAmr6LsXde7frQ6H6vMKg_YSoJU1wxlYQl-mqaHavG2X92MpT666nX0eja97o1If5jJReY4pL8AkVEc2M</recordid><startdate>19930601</startdate><enddate>19930601</enddate><creator>LILLY, P</creator><creator>LIJUN WU</creator><creator>WELKER, D. L</creator><creator>DEVREOTES, P. N</creator><general>Cold Spring Harbor Laboratory</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19930601</creationdate><title>A G-protein β-subunit is essential for Dictyostelium development</title><author>LILLY, P ; LIJUN WU ; WELKER, D. L ; DEVREOTES, P. N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-33f770ebaf5c301f7eb1e68dbbd96022c5f8c558480977031d783df15bda60333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Dictyostelium - genetics</topic><topic>Dictyostelium - growth & development</topic><topic>Dictyostelium discoideum</topic><topic>DNA, Antisense - genetics</topic><topic>DNA, Fungal - analysis</topic><topic>DNA, Fungal - chemistry</topic><topic>DNA, Protozoan - analysis</topic><topic>DNA, Protozoan - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>GTP-Binding Proteins - chemistry</topic><topic>GTP-Binding Proteins - genetics</topic><topic>GTP-Binding Proteins - physiology</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis</topic><topic>Oligonucleotides - chemistry</topic><topic>Peptide Fragments - chemistry</topic><topic>Peptide Fragments - genetics</topic><topic>Peptide Fragments - physiology</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Protozoa</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Signal Transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LILLY, P</creatorcontrib><creatorcontrib>LIJUN WU</creatorcontrib><creatorcontrib>WELKER, D. L</creatorcontrib><creatorcontrib>DEVREOTES, P. N</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Genes & development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LILLY, P</au><au>LIJUN WU</au><au>WELKER, D. L</au><au>DEVREOTES, P. N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A G-protein β-subunit is essential for Dictyostelium development</atitle><jtitle>Genes & development</jtitle><addtitle>Genes Dev</addtitle><date>1993-06-01</date><risdate>1993</risdate><volume>7</volume><issue>6</issue><spage>986</spage><epage>995</epage><pages>986-995</pages><issn>0890-9369</issn><eissn>1549-5477</eissn><coden>GEDEEP</coden><abstract>Recent studies have demonstrated that G-protein-linked signal transduction pathways play a significant role in the developmental program of the simple eukaryotic organism Dictyostelium. We have reported previously the isolation of a G-protein beta-subunit and present here a more complete analysis of this gene. Low-stringency Southern blots and RFLP mapping studies suggest that the beta-subunit is a unique gene found on linkage group II. Its deduced amino acid sequence of 347 residues is approximately 60% identical to those of the human, Drosophila, and Caenorhabditis elegans beta-subunits. The carboxy-terminal 300 residues are about 70% identical; the amino-terminal 50 residues are quite divergent, containing only 10 identities. At all stages of growth and development, a single 1.9-kb beta-subunit mRNA is present at a high level, and a specific antibody detects a single 37-kD protein. We propose that G-protein heterotrimers are formed when this beta-subunit couples with each of the eight distinct G-protein alpha-subunits that are transiently expressed during development. Targeted disruption of the beta-subunit gene had no effect on the viability of haploid cells, but resulted in the inability of cells to aggregate.</abstract><cop>Cold Spring Harbor, NY</cop><pub>Cold Spring Harbor Laboratory</pub><pmid>8099335</pmid><doi>10.1101/gad.7.6.986</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0890-9369 |
| ispartof | Genes & development, 1993-06, Vol.7 (6), p.986-995 |
| issn | 0890-9369 1549-5477 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16673404 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Cell Line Classical genetics, quantitative genetics, hybrids Dictyostelium - genetics Dictyostelium - growth & development Dictyostelium discoideum DNA, Antisense - genetics DNA, Fungal - analysis DNA, Fungal - chemistry DNA, Protozoan - analysis DNA, Protozoan - chemistry Fundamental and applied biological sciences. Psychology Gene Expression Genetics of eukaryotes. Biological and molecular evolution GTP-Binding Proteins - chemistry GTP-Binding Proteins - genetics GTP-Binding Proteins - physiology Molecular Sequence Data Mutagenesis Oligonucleotides - chemistry Peptide Fragments - chemistry Peptide Fragments - genetics Peptide Fragments - physiology Polymorphism, Restriction Fragment Length Protozoa Sequence Alignment Sequence Homology, Amino Acid Signal Transduction |
| title | A G-protein β-subunit is essential for Dictyostelium development |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T00%3A46%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20G-protein%20%CE%B2-subunit%20is%20essential%20for%20Dictyostelium%20development&rft.jtitle=Genes%20&%20development&rft.au=LILLY,%20P&rft.date=1993-06-01&rft.volume=7&rft.issue=6&rft.spage=986&rft.epage=995&rft.pages=986-995&rft.issn=0890-9369&rft.eissn=1549-5477&rft.coden=GEDEEP&rft_id=info:doi/10.1101/gad.7.6.986&rft_dat=%3Cproquest_cross%3E16673404%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16673404&rft_id=info:pmid/8099335&rfr_iscdi=true |