Neoplastic Transformation of C3H Mouse Embryo Cells, 10T1/2: Cell-cycle Dependence for 50 kV X-rays and UV-B Light
The variation of neoplastic transformation induced by 50 kV X-rays, and by solar-simulating UV-B light, was studied through the cell cycle of C3H mouse embryo cells designated 10T1/2. A mitotic shake-off method was used to harvest mitotic cells. The progression through the cell cycle of initially mi...
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| Veröffentlicht in: | International journal of radiation biology 1993, Vol.64 (1), p.83-92 |
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| container_title | International journal of radiation biology |
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| creator | Cao, J. Wells, R.L. Elkind, M.M. |
| description | The variation of neoplastic transformation induced by 50 kV X-rays, and by solar-simulating UV-B light, was studied through the cell cycle of C3H mouse embryo cells designated 10T1/2. A mitotic shake-off method was used to harvest mitotic cells. The progression through the cell cycle of initially mitotic cells was followed as a funciton of time by flow cytometry, DNA labelling for passage through S-phase, and growth curves for cell number. At 2-3 h after shake-off, about 90% of the cells were in early G1-phase and by 15 h 60-70% of cells had reached S-phase. For 2·5 Gy, the transformation frequency per viable cell in M-phase was some five times higher than in S-phase. In contrast, at similar survival levels, UV-B light is less efficient in transforming mitotic cells. For both types of radiation, the frequency of neoplastic transformation per viable cell was roughly inversely proportional to survival. |
| doi_str_mv | 10.1080/09553009314551131 |
| format | Article |
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A mitotic shake-off method was used to harvest mitotic cells. The progression through the cell cycle of initially mitotic cells was followed as a funciton of time by flow cytometry, DNA labelling for passage through S-phase, and growth curves for cell number. At 2-3 h after shake-off, about 90% of the cells were in early G1-phase and by 15 h 60-70% of cells had reached S-phase. For 2·5 Gy, the transformation frequency per viable cell in M-phase was some five times higher than in S-phase. In contrast, at similar survival levels, UV-B light is less efficient in transforming mitotic cells. 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A mitotic shake-off method was used to harvest mitotic cells. The progression through the cell cycle of initially mitotic cells was followed as a funciton of time by flow cytometry, DNA labelling for passage through S-phase, and growth curves for cell number. At 2-3 h after shake-off, about 90% of the cells were in early G1-phase and by 15 h 60-70% of cells had reached S-phase. For 2·5 Gy, the transformation frequency per viable cell in M-phase was some five times higher than in S-phase. In contrast, at similar survival levels, UV-B light is less efficient in transforming mitotic cells. For both types of radiation, the frequency of neoplastic transformation per viable cell was roughly inversely proportional to survival.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>Cell Cycle - physiology</subject><subject>Cell Cycle - radiation effects</subject><subject>Cell Transformation, Neoplastic - radiation effects</subject><subject>Embryo, Mammalian - cytology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Physical agents</subject><subject>Space life sciences</subject><subject>Tumors</subject><subject>Ultraviolet Rays</subject><subject>X-Rays</subject><issn>0955-3002</issn><issn>1362-3095</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EKkvhA3BA8gFxInQmjv9RLrAUirTAZVtxixzHZlOSeLGzQvn2uN2lEkIqJ4_n_d5oRo-QpwivEBScgOacAWiGFeeIDO-RBTJRFiwr98niWs81lA_Jo5SuIFfA1BE5UgglympB4hcXtr1JU2fpOpox-RAHM3VhpMHTJTunn8MuOXo2NHEOdOn6Pr2kCGs8KV_ffAs7297R927rxtaN1tE8gnKgPy7ptyKaOVEztvTisnhHV933zfSYPPCmT-7J4T0mFx_O1svzYvX146fl21VhK4FTwbFlSnPQorHgwCCTDG3uclAlopLgK9bY1llvpBZMayEEb5iUoHxrPTsmL_ZztzH83Lk01UOXbN7YjC7fVEuuNHLF_wuiEJJVgBnEPWhjSCk6X29jN5g41wj1dSD1P4Fkz7PD8F0zuPbWcUgg688PuknW9D5nYLt0i1UKpWYqY2_2WDfeJPQrxL6tJzP3If7xsLu2OP3LvnGmnzbWRFdfhV0ccw533PAbyWWzRA</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>Cao, J.</creator><creator>Wells, R.L.</creator><creator>Elkind, M.M.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>1993</creationdate><title>Neoplastic Transformation of C3H Mouse Embryo Cells, 10T1/2: Cell-cycle Dependence for 50 kV X-rays and UV-B Light</title><author>Cao, J. ; Wells, R.L. ; Elkind, M.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-51d3895096bc0e0a13731c51d508211870f43bcdecfa7963996665b37708fdcf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carcinogenesis, carcinogens and anticarcinogens</topic><topic>Cell Cycle - physiology</topic><topic>Cell Cycle - radiation effects</topic><topic>Cell Transformation, Neoplastic - radiation effects</topic><topic>Embryo, Mammalian - cytology</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Physical agents</topic><topic>Space life sciences</topic><topic>Tumors</topic><topic>Ultraviolet Rays</topic><topic>X-Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cao, J.</creatorcontrib><creatorcontrib>Wells, R.L.</creatorcontrib><creatorcontrib>Elkind, M.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of radiation biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cao, J.</au><au>Wells, R.L.</au><au>Elkind, M.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neoplastic Transformation of C3H Mouse Embryo Cells, 10T1/2: Cell-cycle Dependence for 50 kV X-rays and UV-B Light</atitle><jtitle>International journal of radiation biology</jtitle><addtitle>Int J Radiat Biol</addtitle><date>1993</date><risdate>1993</risdate><volume>64</volume><issue>1</issue><spage>83</spage><epage>92</epage><pages>83-92</pages><issn>0955-3002</issn><eissn>1362-3095</eissn><abstract>The variation of neoplastic transformation induced by 50 kV X-rays, and by solar-simulating UV-B light, was studied through the cell cycle of C3H mouse embryo cells designated 10T1/2. A mitotic shake-off method was used to harvest mitotic cells. The progression through the cell cycle of initially mitotic cells was followed as a funciton of time by flow cytometry, DNA labelling for passage through S-phase, and growth curves for cell number. At 2-3 h after shake-off, about 90% of the cells were in early G1-phase and by 15 h 60-70% of cells had reached S-phase. For 2·5 Gy, the transformation frequency per viable cell in M-phase was some five times higher than in S-phase. In contrast, at similar survival levels, UV-B light is less efficient in transforming mitotic cells. For both types of radiation, the frequency of neoplastic transformation per viable cell was roughly inversely proportional to survival.</abstract><cop>London</cop><pub>Informa UK Ltd</pub><pmid>8102174</pmid><doi>10.1080/09553009314551131</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0955-3002 |
| ispartof | International journal of radiation biology, 1993, Vol.64 (1), p.83-92 |
| issn | 0955-3002 1362-3095 |
| language | eng |
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| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Animals Biological and medical sciences Carcinogenesis, carcinogens and anticarcinogens Cell Cycle - physiology Cell Cycle - radiation effects Cell Transformation, Neoplastic - radiation effects Embryo, Mammalian - cytology Medical sciences Mice Mice, Inbred C3H Physical agents Space life sciences Tumors Ultraviolet Rays X-Rays |
| title | Neoplastic Transformation of C3H Mouse Embryo Cells, 10T1/2: Cell-cycle Dependence for 50 kV X-rays and UV-B Light |
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