Neoplastic Transformation of C3H Mouse Embryo Cells, 10T1/2: Cell-cycle Dependence for 50 kV X-rays and UV-B Light

The variation of neoplastic transformation induced by 50 kV X-rays, and by solar-simulating UV-B light, was studied through the cell cycle of C3H mouse embryo cells designated 10T1/2. A mitotic shake-off method was used to harvest mitotic cells. The progression through the cell cycle of initially mi...

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Veröffentlicht in:International journal of radiation biology 1993, Vol.64 (1), p.83-92
Hauptverfasser: Cao, J., Wells, R.L., Elkind, M.M.
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Sprache:eng
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Zusammenfassung:The variation of neoplastic transformation induced by 50 kV X-rays, and by solar-simulating UV-B light, was studied through the cell cycle of C3H mouse embryo cells designated 10T1/2. A mitotic shake-off method was used to harvest mitotic cells. The progression through the cell cycle of initially mitotic cells was followed as a funciton of time by flow cytometry, DNA labelling for passage through S-phase, and growth curves for cell number. At 2-3 h after shake-off, about 90% of the cells were in early G1-phase and by 15 h 60-70% of cells had reached S-phase. For 2·5 Gy, the transformation frequency per viable cell in M-phase was some five times higher than in S-phase. In contrast, at similar survival levels, UV-B light is less efficient in transforming mitotic cells. For both types of radiation, the frequency of neoplastic transformation per viable cell was roughly inversely proportional to survival.
ISSN:0955-3002
1362-3095
DOI:10.1080/09553009314551131