Construction of isogenic mutants of Pasteurella haemolytica by allelic replacement

We describe methods for the mutagenesis of cloned Pasteurella haemolytica (Gram −) genes and for the construction of P. haemolytica mutants by allelic exchange. We used these methods to construct isogenic mutants of P. haemolytica which no longer synthesize three membrane lipoproteins (Lpp). A singl...

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Veröffentlicht in:	Gene 1994-10, Vol.148 (1), p.101-105
Hauptverfasser:	Murphy, George L., Whitworth, Lisa C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles bacterial proteins beta-Lactamases - genetics Crossing Over, Genetic DNA, Bacterial - analysis Electroporation Gene Dosage Genes, Bacterial genetic transformation homologous recombination lipoproteins Lipoproteins - biosynthesis Lipoproteins - genetics loci lpp locus lpp1 gene lpp2 gene lpp3 gene Mannheimia haemolytica Mannheimia haemolytica - genetics membrane lipoproteins Membrane Proteins - biosynthesis Membrane Proteins - genetics Mutagenesis mutants mutation Pasteurella haemolytica pasteurellosis Plasmids - genetics structural genes Transformation, Bacterial
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