The mitochondrial outer membrane protein Mas22p is essential for protein import and viability of yeast
We have cloned the gene encoding the protein Mas22p, which spans the outer membrane of yeast mitochondria. Cells that completely lack Mas22p are inviable. The plasmid-borne MAS22 gene suppresses several defects resulting from the deletion of one or more of the mitochondrial protein import receptors....
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1994-12, Vol.91 (25), p.11973-11977 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Lithgow, T. (Universitat Basel, Basel, Switzerland) Junne, T Suda, K Gratzer, S Schatz, G |
| description | We have cloned the gene encoding the protein Mas22p, which spans the outer membrane of yeast mitochondria. Cells that completely lack Mas22p are inviable. The plasmid-borne MAS22 gene suppresses several defects resulting from the deletion of one or more of the mitochondrial protein import receptors. Defects of Mas20p-deficient cells are explained by the reduced level of Mas22p in these mutants. Mas22p has one acidic domain in the cytosol and a second acidic domain in the mitochondrial intermembrane space. We suggest that these domains of Mas22p on either side of the outer membrane function as a relay system for transferring the basic targeting sequences of precursor proteins into the mitochondria. |
| doi_str_mv | 10.1073/pnas.91.25.11973 |
| format | Article |
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(Universitat Basel, Basel, Switzerland) ; Junne, T ; Suda, K ; Gratzer, S ; Schatz, G</creator><creatorcontrib>Lithgow, T. (Universitat Basel, Basel, Switzerland) ; Junne, T ; Suda, K ; Gratzer, S ; Schatz, G</creatorcontrib><description>We have cloned the gene encoding the protein Mas22p, which spans the outer membrane of yeast mitochondria. Cells that completely lack Mas22p are inviable. The plasmid-borne MAS22 gene suppresses several defects resulting from the deletion of one or more of the mitochondrial protein import receptors. Defects of Mas20p-deficient cells are explained by the reduced level of Mas22p in these mutants. Mas22p has one acidic domain in the cytosol and a second acidic domain in the mitochondrial intermembrane space. We suggest that these domains of Mas22p on either side of the outer membrane function as a relay system for transferring the basic targeting sequences of precursor proteins into the mitochondria.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.91.25.11973</identifier><identifier>PMID: 7991567</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Amino Acid Sequence ; Cell growth ; Cells ; Cellular biology ; Chromosomes, Fungal ; Cloning, Molecular ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; DNA, Fungal - metabolism ; Fungal Proteins - biosynthesis ; Fungal Proteins - isolation & purification ; Fungal Proteins - metabolism ; GENE ; GENES ; Genes, Fungal ; Genetics ; Imports ; Intracellular Membranes - metabolism ; Intracellular Membranes - ultrastructure ; MEMBRANAS CELULARES ; MEMBRANE CELLULAIRE ; Membrane proteins ; Membrane Proteins - biosynthesis ; Membrane Proteins - isolation & purification ; Membrane Proteins - metabolism ; Membrane Transport Proteins ; METABOLISME DES PROTEINES ; METABOLISMO PROTEICO ; Mitochondria ; Mitochondria - metabolism ; Mitochondrial Membrane Transport Proteins ; MITOCHONDRIE ; MITOCONDRIA ; Models, Structural ; Molecular Sequence Data ; MUTANT ; MUTANTES ; Plasmids ; Protein precursors ; Protein Processing, Post-Translational ; PROTEINAS ; PROTEINE ; Proteins ; Receptors ; Receptors, Cell Surface ; Respiration ; Restriction Mapping ; SACCHAROMYCES CEREVISIAE ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - growth & development ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins ; SECUENCIA NUCLEICA ; Sequence Homology, Amino Acid ; SEQUENCE NUCLEIQUE ; VIABILIDAD ; VIABILITE ; Yeast ; Yeasts</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1994-12, Vol.91 (25), p.11973-11977</ispartof><rights>Copyright 1994 The National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Dec 6, 1994</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c610t-661c03255d443a48d7d2e720e657a356cdb3606c7374b9804329e24bb95dc093</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/91/25.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2366259$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2366259$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7991567$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lithgow, T. (Universitat Basel, Basel, Switzerland)</creatorcontrib><creatorcontrib>Junne, T</creatorcontrib><creatorcontrib>Suda, K</creatorcontrib><creatorcontrib>Gratzer, S</creatorcontrib><creatorcontrib>Schatz, G</creatorcontrib><title>The mitochondrial outer membrane protein Mas22p is essential for protein import and viability of yeast</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We have cloned the gene encoding the protein Mas22p, which spans the outer membrane of yeast mitochondria. Cells that completely lack Mas22p are inviable. The plasmid-borne MAS22 gene suppresses several defects resulting from the deletion of one or more of the mitochondrial protein import receptors. Defects of Mas20p-deficient cells are explained by the reduced level of Mas22p in these mutants. Mas22p has one acidic domain in the cytosol and a second acidic domain in the mitochondrial intermembrane space. We suggest that these domains of Mas22p on either side of the outer membrane function as a relay system for transferring the basic targeting sequences of precursor proteins into the mitochondria.</description><subject>Amino Acid Sequence</subject><subject>Cell growth</subject><subject>Cells</subject><subject>Cellular biology</subject><subject>Chromosomes, Fungal</subject><subject>Cloning, Molecular</subject><subject>COMPOSICION QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>DNA, Fungal - metabolism</subject><subject>Fungal Proteins - biosynthesis</subject><subject>Fungal Proteins - isolation & purification</subject><subject>Fungal Proteins - metabolism</subject><subject>GENE</subject><subject>GENES</subject><subject>Genes, Fungal</subject><subject>Genetics</subject><subject>Imports</subject><subject>Intracellular Membranes - metabolism</subject><subject>Intracellular Membranes - ultrastructure</subject><subject>MEMBRANAS CELULARES</subject><subject>MEMBRANE CELLULAIRE</subject><subject>Membrane proteins</subject><subject>Membrane Proteins - biosynthesis</subject><subject>Membrane Proteins - isolation & purification</subject><subject>Membrane Proteins - metabolism</subject><subject>Membrane Transport Proteins</subject><subject>METABOLISME DES PROTEINES</subject><subject>METABOLISMO PROTEICO</subject><subject>Mitochondria</subject><subject>Mitochondria - metabolism</subject><subject>Mitochondrial Membrane Transport Proteins</subject><subject>MITOCHONDRIE</subject><subject>MITOCONDRIA</subject><subject>Models, Structural</subject><subject>Molecular Sequence Data</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>Plasmids</subject><subject>Protein precursors</subject><subject>Protein Processing, Post-Translational</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>Proteins</subject><subject>Receptors</subject><subject>Receptors, Cell Surface</subject><subject>Respiration</subject><subject>Restriction Mapping</subject><subject>SACCHAROMYCES CEREVISIAE</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - growth & development</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>SECUENCIA NUCLEICA</subject><subject>Sequence Homology, Amino Acid</subject><subject>SEQUENCE NUCLEIQUE</subject><subject>VIABILIDAD</subject><subject>VIABILITE</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EKtPCHiEQFgvEJoPfD4lNVfGSilgwrC0ncToeJXGwnYr59zjMMKIsYHUX5ztX954DwBOM1hhJ-mYabVprvCZ8jbGW9B5YYaRxJZhG98EKISIrxQh7CM5T2iGENFfoDJxJrTEXcgW6zdbBwefQbMPYRm97GObsIhzcUEc7OjjFkJ0f4WebCJmgT9Cl5Ma8oF2IJ90PU4gZ2rGFt97Wvvd5D0MH986m_Ag86Gyf3OPjvACb9-82Vx-r6y8fPl1dXleNwChXQuAGUcJ5yxi1TLWyJU4S5ASXlnLRtDUVSDSSSlZrhRgl2hFW15q3DdL0Arw9rJ3menBtU86MtjdT9IONexOsN3eV0W_NTbg1jFOuiv3V0R7D99mlbAafGtf3JYcwJyOFUpgj8l8QCyE0IbiAL_8Cd2GOY4nAEIQJJ1rzAqED1MSQUnTd6WCMzFKzWWo2GhvCza-ai-X5n4-eDMdei_7iqC_O3-rdDa__TZhu7vvsfuSCPjugu5RDPLGECkH4EvrTg9zZYOxN9Ml8-1reUkor-hNigM51</recordid><startdate>19941206</startdate><enddate>19941206</enddate><creator>Lithgow, T. (Universitat Basel, Basel, Switzerland)</creator><creator>Junne, T</creator><creator>Suda, K</creator><creator>Gratzer, S</creator><creator>Schatz, G</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19941206</creationdate><title>The mitochondrial outer membrane protein Mas22p is essential for protein import and viability of yeast</title><author>Lithgow, T. (Universitat Basel, Basel, Switzerland) ; Junne, T ; Suda, K ; Gratzer, S ; Schatz, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c610t-661c03255d443a48d7d2e720e657a356cdb3606c7374b9804329e24bb95dc093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>Cell growth</topic><topic>Cells</topic><topic>Cellular biology</topic><topic>Chromosomes, Fungal</topic><topic>Cloning, Molecular</topic><topic>COMPOSICION QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>DNA, Fungal - metabolism</topic><topic>Fungal Proteins - biosynthesis</topic><topic>Fungal Proteins - isolation & purification</topic><topic>Fungal Proteins - metabolism</topic><topic>GENE</topic><topic>GENES</topic><topic>Genes, Fungal</topic><topic>Genetics</topic><topic>Imports</topic><topic>Intracellular Membranes - metabolism</topic><topic>Intracellular Membranes - ultrastructure</topic><topic>MEMBRANAS CELULARES</topic><topic>MEMBRANE CELLULAIRE</topic><topic>Membrane proteins</topic><topic>Membrane Proteins - biosynthesis</topic><topic>Membrane Proteins - isolation & purification</topic><topic>Membrane Proteins - metabolism</topic><topic>Membrane Transport Proteins</topic><topic>METABOLISME DES PROTEINES</topic><topic>METABOLISMO PROTEICO</topic><topic>Mitochondria</topic><topic>Mitochondria - metabolism</topic><topic>Mitochondrial Membrane Transport Proteins</topic><topic>MITOCHONDRIE</topic><topic>MITOCONDRIA</topic><topic>Models, Structural</topic><topic>Molecular Sequence Data</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>Plasmids</topic><topic>Protein precursors</topic><topic>Protein Processing, Post-Translational</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>Proteins</topic><topic>Receptors</topic><topic>Receptors, Cell Surface</topic><topic>Respiration</topic><topic>Restriction Mapping</topic><topic>SACCHAROMYCES CEREVISIAE</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - growth & development</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>SECUENCIA NUCLEICA</topic><topic>Sequence Homology, Amino Acid</topic><topic>SEQUENCE NUCLEIQUE</topic><topic>VIABILIDAD</topic><topic>VIABILITE</topic><topic>Yeast</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lithgow, T. 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(Universitat Basel, Basel, Switzerland)</au><au>Junne, T</au><au>Suda, K</au><au>Gratzer, S</au><au>Schatz, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The mitochondrial outer membrane protein Mas22p is essential for protein import and viability of yeast</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1994-12-06</date><risdate>1994</risdate><volume>91</volume><issue>25</issue><spage>11973</spage><epage>11977</epage><pages>11973-11977</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>We have cloned the gene encoding the protein Mas22p, which spans the outer membrane of yeast mitochondria. Cells that completely lack Mas22p are inviable. The plasmid-borne MAS22 gene suppresses several defects resulting from the deletion of one or more of the mitochondrial protein import receptors. Defects of Mas20p-deficient cells are explained by the reduced level of Mas22p in these mutants. Mas22p has one acidic domain in the cytosol and a second acidic domain in the mitochondrial intermembrane space. We suggest that these domains of Mas22p on either side of the outer membrane function as a relay system for transferring the basic targeting sequences of precursor proteins into the mitochondria.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>7991567</pmid><doi>10.1073/pnas.91.25.11973</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1994-12, Vol.91 (25), p.11973-11977 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16669221 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Cell growth Cells Cellular biology Chromosomes, Fungal Cloning, Molecular COMPOSICION QUIMICA COMPOSITION CHIMIQUE DNA, Fungal - metabolism Fungal Proteins - biosynthesis Fungal Proteins - isolation & purification Fungal Proteins - metabolism GENE GENES Genes, Fungal Genetics Imports Intracellular Membranes - metabolism Intracellular Membranes - ultrastructure MEMBRANAS CELULARES MEMBRANE CELLULAIRE Membrane proteins Membrane Proteins - biosynthesis Membrane Proteins - isolation & purification Membrane Proteins - metabolism Membrane Transport Proteins METABOLISME DES PROTEINES METABOLISMO PROTEICO Mitochondria Mitochondria - metabolism Mitochondrial Membrane Transport Proteins MITOCHONDRIE MITOCONDRIA Models, Structural Molecular Sequence Data MUTANT MUTANTES Plasmids Protein precursors Protein Processing, Post-Translational PROTEINAS PROTEINE Proteins Receptors Receptors, Cell Surface Respiration Restriction Mapping SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins SECUENCIA NUCLEICA Sequence Homology, Amino Acid SEQUENCE NUCLEIQUE VIABILIDAD VIABILITE Yeast Yeasts |
| title | The mitochondrial outer membrane protein Mas22p is essential for protein import and viability of yeast |
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