Relationship between the binding sites for an α-conotoxin and snake venom neurotoxins in the nicotinic acetylcholine receptor from Torpedo californica
Photoinduced cross-links between the iodinated Lys26- p-azidobenzoyl derivative of neurotoxin II from Naja naja oxiana cobra venom and nicotinic acetylcholine receptor from Torpedo californica (AChR) have been studied in the presence of α-conotoxin GI from the marine snail C. geographus. Preincubati...
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| Veröffentlicht in: | Toxicon (Oxford) 1994-09, Vol.32 (9), p.1153-1157 |
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| container_title | Toxicon (Oxford) |
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| creator | Utkin, Yu.N. Kobayashi, Y. Hucho, F. Tsetlin, V.I. |
| description | Photoinduced cross-links between the iodinated Lys26-
p-azidobenzoyl derivative of neurotoxin II from
Naja naja oxiana cobra venom and nicotinic acetylcholine receptor from
Torpedo californica (AChR) have been studied in the presence of α-conotoxin GI from the marine snail
C. geographus. Preincubation of the AChR-enriched membranes with increasing concentrations of α-conotoxin GI protects first the γ subunit from photo-labelling and then the δ subunit, the
ic
50 values being 0.76 and 5.01 μM, respectively. The results obtained, in view of the relevant data in literature, demonstrate that the (
α +
γ) site, which is the high affinity site for
d-tubocurarine, has also a higher affinity for an α-conotoxin than the (
α +
δ) containing site. The latter has a somewhat higher affinity than the (
α +
γ) site towards some naturally occurring snake venom α-neurotoxins or their derivatives. |
| doi_str_mv | 10.1016/0041-0101(94)90399-9 |
| format | Article |
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p-azidobenzoyl derivative of neurotoxin II from
Naja naja oxiana cobra venom and nicotinic acetylcholine receptor from
Torpedo californica (AChR) have been studied in the presence of α-conotoxin GI from the marine snail
C. geographus. Preincubation of the AChR-enriched membranes with increasing concentrations of α-conotoxin GI protects first the γ subunit from photo-labelling and then the δ subunit, the
ic
50 values being 0.76 and 5.01 μM, respectively. The results obtained, in view of the relevant data in literature, demonstrate that the (
α +
γ) site, which is the high affinity site for
d-tubocurarine, has also a higher affinity for an α-conotoxin than the (
α +
δ) containing site. The latter has a somewhat higher affinity than the (
α +
γ) site towards some naturally occurring snake venom α-neurotoxins or their derivatives.</description><identifier>ISSN: 0041-0101</identifier><identifier>EISSN: 1879-3150</identifier><identifier>DOI: 10.1016/0041-0101(94)90399-9</identifier><identifier>PMID: 7801351</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Binding Sites ; Binding, Competitive ; Conotoxins ; Cross-Linking Reagents ; Elapid Venoms - metabolism ; Elapid Venoms - toxicity ; Elapidae ; Mollusk Venoms - metabolism ; Mollusk Venoms - toxicity ; Neurotoxins - metabolism ; Neurotoxins - toxicity ; Peptides, Cyclic - metabolism ; Peptides, Cyclic - toxicity ; Receptors, Nicotinic - drug effects ; Receptors, Nicotinic - metabolism ; Snails ; Torpedo - metabolism ; Torpedo californica</subject><ispartof>Toxicon (Oxford), 1994-09, Vol.32 (9), p.1153-1157</ispartof><rights>1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-b91fbc7863cca71f2c6756c66ad47d1907ffa3d64b67c724e9b2b763e30079283</citedby><cites>FETCH-LOGICAL-c388t-b91fbc7863cca71f2c6756c66ad47d1907ffa3d64b67c724e9b2b763e30079283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0041010194903999$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7801351$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Utkin, Yu.N.</creatorcontrib><creatorcontrib>Kobayashi, Y.</creatorcontrib><creatorcontrib>Hucho, F.</creatorcontrib><creatorcontrib>Tsetlin, V.I.</creatorcontrib><title>Relationship between the binding sites for an α-conotoxin and snake venom neurotoxins in the nicotinic acetylcholine receptor from Torpedo californica</title><title>Toxicon (Oxford)</title><addtitle>Toxicon</addtitle><description>Photoinduced cross-links between the iodinated Lys26-
p-azidobenzoyl derivative of neurotoxin II from
Naja naja oxiana cobra venom and nicotinic acetylcholine receptor from
Torpedo californica (AChR) have been studied in the presence of α-conotoxin GI from the marine snail
C. geographus. Preincubation of the AChR-enriched membranes with increasing concentrations of α-conotoxin GI protects first the γ subunit from photo-labelling and then the δ subunit, the
ic
50 values being 0.76 and 5.01 μM, respectively. The results obtained, in view of the relevant data in literature, demonstrate that the (
α +
γ) site, which is the high affinity site for
d-tubocurarine, has also a higher affinity for an α-conotoxin than the (
α +
δ) containing site. The latter has a somewhat higher affinity than the (
α +
γ) site towards some naturally occurring snake venom α-neurotoxins or their derivatives.</description><subject>Animals</subject><subject>Binding Sites</subject><subject>Binding, Competitive</subject><subject>Conotoxins</subject><subject>Cross-Linking Reagents</subject><subject>Elapid Venoms - metabolism</subject><subject>Elapid Venoms - toxicity</subject><subject>Elapidae</subject><subject>Mollusk Venoms - metabolism</subject><subject>Mollusk Venoms - toxicity</subject><subject>Neurotoxins - metabolism</subject><subject>Neurotoxins - toxicity</subject><subject>Peptides, Cyclic - metabolism</subject><subject>Peptides, Cyclic - toxicity</subject><subject>Receptors, Nicotinic - drug effects</subject><subject>Receptors, Nicotinic - metabolism</subject><subject>Snails</subject><subject>Torpedo - metabolism</subject><subject>Torpedo californica</subject><issn>0041-0101</issn><issn>1879-3150</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Ud1uFCEUJsamrtU30IQroxdTYZmB4cbENGqbNGli6jVhDmdcdBZGYGv7JH0OX8Rnku1seukNcPL9nPB9hLzi7JQzLt8z1vKG1edb3b7TTGjd6CdkxXulG8E79pSsHinPyPOcfzDGRK_lMTlWPeOi4yty_xUnW3wMeeNnOmD5jRho2SAdfHA-fKfZF8x0jInaQP_-aSCGWOKtD3V2NAf7E-kNhrilAXdpgTL1i0nwEIuvJ7WA5W6CTZx8QJoQcC7Vc0xVeB3TjC5SsJOviyrdviBHo50yvjzcJ-Tb50_XZ-fN5dWXi7OPlw2Ivi_NoPk4gOqlALCKj2uQqpMgpXWtclwzNY5WONkOUoFat6iH9aCkQMGY0utenJA3i--c4q8d5mK2PgNOkw0Yd9lwKbued6oS24UIKeaccDRz8lub7gxnZt-H2Ydt9mEb3ZqHPoyustcH_92wRfcoOhRQ8Q8LjvWTNx6TyeAxADpfMyrGRf__Bf8ATmieJw</recordid><startdate>19940901</startdate><enddate>19940901</enddate><creator>Utkin, Yu.N.</creator><creator>Kobayashi, Y.</creator><creator>Hucho, F.</creator><creator>Tsetlin, V.I.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>19940901</creationdate><title>Relationship between the binding sites for an α-conotoxin and snake venom neurotoxins in the nicotinic acetylcholine receptor from Torpedo californica</title><author>Utkin, Yu.N. ; Kobayashi, Y. ; Hucho, F. ; Tsetlin, V.I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-b91fbc7863cca71f2c6756c66ad47d1907ffa3d64b67c724e9b2b763e30079283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Binding Sites</topic><topic>Binding, Competitive</topic><topic>Conotoxins</topic><topic>Cross-Linking Reagents</topic><topic>Elapid Venoms - metabolism</topic><topic>Elapid Venoms - toxicity</topic><topic>Elapidae</topic><topic>Mollusk Venoms - metabolism</topic><topic>Mollusk Venoms - toxicity</topic><topic>Neurotoxins - metabolism</topic><topic>Neurotoxins - toxicity</topic><topic>Peptides, Cyclic - metabolism</topic><topic>Peptides, Cyclic - toxicity</topic><topic>Receptors, Nicotinic - drug effects</topic><topic>Receptors, Nicotinic - metabolism</topic><topic>Snails</topic><topic>Torpedo - metabolism</topic><topic>Torpedo californica</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Utkin, Yu.N.</creatorcontrib><creatorcontrib>Kobayashi, Y.</creatorcontrib><creatorcontrib>Hucho, F.</creatorcontrib><creatorcontrib>Tsetlin, V.I.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Toxicon (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Utkin, Yu.N.</au><au>Kobayashi, Y.</au><au>Hucho, F.</au><au>Tsetlin, V.I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relationship between the binding sites for an α-conotoxin and snake venom neurotoxins in the nicotinic acetylcholine receptor from Torpedo californica</atitle><jtitle>Toxicon (Oxford)</jtitle><addtitle>Toxicon</addtitle><date>1994-09-01</date><risdate>1994</risdate><volume>32</volume><issue>9</issue><spage>1153</spage><epage>1157</epage><pages>1153-1157</pages><issn>0041-0101</issn><eissn>1879-3150</eissn><abstract>Photoinduced cross-links between the iodinated Lys26-
p-azidobenzoyl derivative of neurotoxin II from
Naja naja oxiana cobra venom and nicotinic acetylcholine receptor from
Torpedo californica (AChR) have been studied in the presence of α-conotoxin GI from the marine snail
C. geographus. Preincubation of the AChR-enriched membranes with increasing concentrations of α-conotoxin GI protects first the γ subunit from photo-labelling and then the δ subunit, the
ic
50 values being 0.76 and 5.01 μM, respectively. The results obtained, in view of the relevant data in literature, demonstrate that the (
α +
γ) site, which is the high affinity site for
d-tubocurarine, has also a higher affinity for an α-conotoxin than the (
α +
δ) containing site. The latter has a somewhat higher affinity than the (
α +
γ) site towards some naturally occurring snake venom α-neurotoxins or their derivatives.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>7801351</pmid><doi>10.1016/0041-0101(94)90399-9</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0041-0101 |
| ispartof | Toxicon (Oxford), 1994-09, Vol.32 (9), p.1153-1157 |
| issn | 0041-0101 1879-3150 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16658157 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Binding Sites Binding, Competitive Conotoxins Cross-Linking Reagents Elapid Venoms - metabolism Elapid Venoms - toxicity Elapidae Mollusk Venoms - metabolism Mollusk Venoms - toxicity Neurotoxins - metabolism Neurotoxins - toxicity Peptides, Cyclic - metabolism Peptides, Cyclic - toxicity Receptors, Nicotinic - drug effects Receptors, Nicotinic - metabolism Snails Torpedo - metabolism Torpedo californica |
| title | Relationship between the binding sites for an α-conotoxin and snake venom neurotoxins in the nicotinic acetylcholine receptor from Torpedo californica |
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