Construction of conjugative shuttle and suicide vectors for Pasteurella haemolytica and P. multocida
A shuttle cloning vector, pAKA16, and suicide derivatives pAKA19 and pAKA22 have been developed for gene transfer to Pasteurella haemolytica and P. multocida. pAKA16 was constructed by insertion of the lacZα-peptide-encoding region and a multiple cloning site into a plasmid which was originally isol...
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| Veröffentlicht in: | Gene 1994-07, Vol.145 (1), p.81-85 |
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| creator | Azad, Abul K. Coote, John G. Parton, Roger |
| description | A shuttle cloning vector, pAKA16, and suicide derivatives pAKA19 and pAKA22 have been developed for gene transfer to
Pasteurella haemolytica and
P. multocida. pAKA16 was constructed by insertion of the
lacZα-peptide-encoding region and a multiple cloning site into a plasmid which was originally isolated from
P. haemolytica serotype A1. The vector encodes ampicillin resistance, and contains at least 14 unique restriction sites and the property of phenotypic identification of recombinant clones in
Escherichia coli by insertional inactivation of β-galactosidase activity. It can be transferred by conjugation to
P. haemolytica or
P. multocida and is stably maintained in both species. The type-II chloramphenicol acetyltransferase-encoding gene (cat), cloned into pAKA16, was stably expressed in both
P. haemolytica and
P. multocida. Plasmids pAKA19 and pAKA22 were constructed by replacement of the origin of DNA replication (
ori) of pAKA16 with a ColEl-type
ori from pBR322 or an
ori of plasmid R6K (
oriR6K) from pJM703.1, respectively. These derivatives replicate in
E. coli, but not in either
P. haemolytica or
P. multocida, and are suitable for use as suicide vectors for these
Pasteurella species. |
| doi_str_mv | 10.1016/0378-1119(94)90326-3 |
| format | Article |
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Pasteurella haemolytica and
P. multocida. pAKA16 was constructed by insertion of the
lacZα-peptide-encoding region and a multiple cloning site into a plasmid which was originally isolated from
P. haemolytica serotype A1. The vector encodes ampicillin resistance, and contains at least 14 unique restriction sites and the property of phenotypic identification of recombinant clones in
Escherichia coli by insertional inactivation of β-galactosidase activity. It can be transferred by conjugation to
P. haemolytica or
P. multocida and is stably maintained in both species. The type-II chloramphenicol acetyltransferase-encoding gene (cat), cloned into pAKA16, was stably expressed in both
P. haemolytica and
P. multocida. Plasmids pAKA19 and pAKA22 were constructed by replacement of the origin of DNA replication (
ori) of pAKA16 with a ColEl-type
ori from pBR322 or an
ori of plasmid R6K (
oriR6K) from pJM703.1, respectively. These derivatives replicate in
E. coli, but not in either
P. haemolytica or
P. multocida, and are suitable for use as suicide vectors for these
Pasteurella species.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(94)90326-3</identifier><identifier>PMID: 8045428</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>chloramphenicol acetyltransferase ; Chloramphenicol O-Acetyltransferase ; Cloning vector ; Cloning, Molecular ; ColEl-type replicon, oriR6K replicon ; conjugation ; Conjugation, Genetic ; conjugative transfer ; Escherichia coli - genetics ; gene expression ; gene transfer ; genetic transformation ; Genetic Vectors ; lacZα ; Mannheimia haemolytica ; Mannheimia haemolytica - genetics ; mob gene ; multiple cloning site ; Pasteurella haemolytica ; Pasteurella multocida ; Pasteurella multocida - genetics ; plasmid vectors ; Plasmids ; reporter genes ; Restriction Mapping</subject><ispartof>Gene, 1994-07, Vol.145 (1), p.81-85</ispartof><rights>1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-55ce7f6224774da743bfef333ee5f9e88c9a089be183db11ad9a05eb8607dc573</citedby><cites>FETCH-LOGICAL-c381t-55ce7f6224774da743bfef333ee5f9e88c9a089be183db11ad9a05eb8607dc573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(94)90326-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8045428$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Azad, Abul K.</creatorcontrib><creatorcontrib>Coote, John G.</creatorcontrib><creatorcontrib>Parton, Roger</creatorcontrib><title>Construction of conjugative shuttle and suicide vectors for Pasteurella haemolytica and P. multocida</title><title>Gene</title><addtitle>Gene</addtitle><description>A shuttle cloning vector, pAKA16, and suicide derivatives pAKA19 and pAKA22 have been developed for gene transfer to
Pasteurella haemolytica and
P. multocida. pAKA16 was constructed by insertion of the
lacZα-peptide-encoding region and a multiple cloning site into a plasmid which was originally isolated from
P. haemolytica serotype A1. The vector encodes ampicillin resistance, and contains at least 14 unique restriction sites and the property of phenotypic identification of recombinant clones in
Escherichia coli by insertional inactivation of β-galactosidase activity. It can be transferred by conjugation to
P. haemolytica or
P. multocida and is stably maintained in both species. The type-II chloramphenicol acetyltransferase-encoding gene (cat), cloned into pAKA16, was stably expressed in both
P. haemolytica and
P. multocida. Plasmids pAKA19 and pAKA22 were constructed by replacement of the origin of DNA replication (
ori) of pAKA16 with a ColEl-type
ori from pBR322 or an
ori of plasmid R6K (
oriR6K) from pJM703.1, respectively. These derivatives replicate in
E. coli, but not in either
P. haemolytica or
P. multocida, and are suitable for use as suicide vectors for these
Pasteurella species.</description><subject>chloramphenicol acetyltransferase</subject><subject>Chloramphenicol O-Acetyltransferase</subject><subject>Cloning vector</subject><subject>Cloning, Molecular</subject><subject>ColEl-type replicon, oriR6K replicon</subject><subject>conjugation</subject><subject>Conjugation, Genetic</subject><subject>conjugative transfer</subject><subject>Escherichia coli - genetics</subject><subject>gene expression</subject><subject>gene transfer</subject><subject>genetic transformation</subject><subject>Genetic Vectors</subject><subject>lacZα</subject><subject>Mannheimia haemolytica</subject><subject>Mannheimia haemolytica - genetics</subject><subject>mob gene</subject><subject>multiple cloning site</subject><subject>Pasteurella haemolytica</subject><subject>Pasteurella multocida</subject><subject>Pasteurella multocida - genetics</subject><subject>plasmid vectors</subject><subject>Plasmids</subject><subject>reporter genes</subject><subject>Restriction Mapping</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVpSTdp_0FLdSrpwalkSbZ0CZSlXxBooM1ZyNIoUbCtVB8L-ffVZpccO5dhmOcdhgehd5RcUEKHz4SNsqOUqnPFPynC-qFjL9CGylF1hDD5Em2ekdfoNOd70kqI_gSdSMIF7-UGuW1cc0nVlhBXHD22cb2vt6aEHeB8V0uZAZvV4VyDDQ7wDmyJKWMfE742uUBNMM8G3xlY4vxYgjVP_PUFXupcYguZN-iVN3OGt8d-hm6-ff2z_dFd_fr-c_vlqrNM0tIJYWH0Q9_zceTOjJxNHjxjDEB4BVJaZYhUE1DJ3ESpcW0WMMmBjM6KkZ2hj4e7Dyn-rZCLXkK2-_dWiDVrOgxCUqIayA-gTTHnBF4_pLCY9Kgp0Xu5em9O781pxfWTXM1a7P3xfp0WcM-ho822_3DYexO1uU0h65vfPaGMUK44JUMjLg8ENA27AElnG2C14EJqYrWL4f8v_AORIZOc</recordid><startdate>19940722</startdate><enddate>19940722</enddate><creator>Azad, Abul K.</creator><creator>Coote, John G.</creator><creator>Parton, Roger</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope></search><sort><creationdate>19940722</creationdate><title>Construction of conjugative shuttle and suicide vectors for Pasteurella haemolytica and P. multocida</title><author>Azad, Abul K. ; Coote, John G. ; Parton, Roger</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-55ce7f6224774da743bfef333ee5f9e88c9a089be183db11ad9a05eb8607dc573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>chloramphenicol acetyltransferase</topic><topic>Chloramphenicol O-Acetyltransferase</topic><topic>Cloning vector</topic><topic>Cloning, Molecular</topic><topic>ColEl-type replicon, oriR6K replicon</topic><topic>conjugation</topic><topic>Conjugation, Genetic</topic><topic>conjugative transfer</topic><topic>Escherichia coli - genetics</topic><topic>gene expression</topic><topic>gene transfer</topic><topic>genetic transformation</topic><topic>Genetic Vectors</topic><topic>lacZα</topic><topic>Mannheimia haemolytica</topic><topic>Mannheimia haemolytica - genetics</topic><topic>mob gene</topic><topic>multiple cloning site</topic><topic>Pasteurella haemolytica</topic><topic>Pasteurella multocida</topic><topic>Pasteurella multocida - genetics</topic><topic>plasmid vectors</topic><topic>Plasmids</topic><topic>reporter genes</topic><topic>Restriction Mapping</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Azad, Abul K.</creatorcontrib><creatorcontrib>Coote, John G.</creatorcontrib><creatorcontrib>Parton, Roger</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Azad, Abul K.</au><au>Coote, John G.</au><au>Parton, Roger</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of conjugative shuttle and suicide vectors for Pasteurella haemolytica and P. multocida</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1994-07-22</date><risdate>1994</risdate><volume>145</volume><issue>1</issue><spage>81</spage><epage>85</epage><pages>81-85</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>A shuttle cloning vector, pAKA16, and suicide derivatives pAKA19 and pAKA22 have been developed for gene transfer to
Pasteurella haemolytica and
P. multocida. pAKA16 was constructed by insertion of the
lacZα-peptide-encoding region and a multiple cloning site into a plasmid which was originally isolated from
P. haemolytica serotype A1. The vector encodes ampicillin resistance, and contains at least 14 unique restriction sites and the property of phenotypic identification of recombinant clones in
Escherichia coli by insertional inactivation of β-galactosidase activity. It can be transferred by conjugation to
P. haemolytica or
P. multocida and is stably maintained in both species. The type-II chloramphenicol acetyltransferase-encoding gene (cat), cloned into pAKA16, was stably expressed in both
P. haemolytica and
P. multocida. Plasmids pAKA19 and pAKA22 were constructed by replacement of the origin of DNA replication (
ori) of pAKA16 with a ColEl-type
ori from pBR322 or an
ori of plasmid R6K (
oriR6K) from pJM703.1, respectively. These derivatives replicate in
E. coli, but not in either
P. haemolytica or
P. multocida, and are suitable for use as suicide vectors for these
Pasteurella species.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8045428</pmid><doi>10.1016/0378-1119(94)90326-3</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1994-07, Vol.145 (1), p.81-85 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16658109 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | chloramphenicol acetyltransferase Chloramphenicol O-Acetyltransferase Cloning vector Cloning, Molecular ColEl-type replicon, oriR6K replicon conjugation Conjugation, Genetic conjugative transfer Escherichia coli - genetics gene expression gene transfer genetic transformation Genetic Vectors lacZα Mannheimia haemolytica Mannheimia haemolytica - genetics mob gene multiple cloning site Pasteurella haemolytica Pasteurella multocida Pasteurella multocida - genetics plasmid vectors Plasmids reporter genes Restriction Mapping |
| title | Construction of conjugative shuttle and suicide vectors for Pasteurella haemolytica and P. multocida |
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