Micronucleus formation in cultured human keratinocytes following exposure to mitomycin C and cyclophosphamide
A method is described to investigate the induction of micronuclei in cultured human keratinocytes after short-term exposure to known clastogenic agents. The cytokinesis-block method was applied to facilitate the scoring of micronucleated cells. Mitomycin C, a direct-acting compound, caused a 5–20-fo...
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| Veröffentlicht in: | Mutation Research 1991-02, Vol.252 (1), p.45-50 |
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| container_title | Mutation Research |
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| creator | van Pelt, Frank N.A.M. Haring, RenéM. Overkamp, Miep J.I. Weterings, Peter J.J.M. |
| description | A method is described to investigate the induction of micronuclei in cultured human keratinocytes after short-term exposure to known clastogenic agents. The cytokinesis-block method was applied to facilitate the scoring of micronucleated cells. Mitomycin C, a direct-acting compound, caused a 5–20-fold increase in micronuclei over the controls at the highest concentration tested (1 μg/ml). Cyclophosphamide, an agent requiring metabolic activation, did not induce the formation of micronuclei in cultured keratinocytes. However, after pretreatment of the keratinocyte cultures with Aroclor 1254 for 72 h, exposure to cyclophosphamide resulted in a 3-fold increase in micronucleus frequency over the controls. No cytogenetic effect of Aroclor 1254 was observed in control experiments. |
| doi_str_mv | 10.1016/0165-1161(91)90250-C |
| format | Article |
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The cytokinesis-block method was applied to facilitate the scoring of micronucleated cells. Mitomycin C, a direct-acting compound, caused a 5–20-fold increase in micronuclei over the controls at the highest concentration tested (1 μg/ml). Cyclophosphamide, an agent requiring metabolic activation, did not induce the formation of micronuclei in cultured keratinocytes. However, after pretreatment of the keratinocyte cultures with Aroclor 1254 for 72 h, exposure to cyclophosphamide resulted in a 3-fold increase in micronucleus frequency over the controls. No cytogenetic effect of Aroclor 1254 was observed in control experiments.</description><identifier>ISSN: 0165-1161</identifier><identifier>ISSN: 0027-5107</identifier><identifier>DOI: 10.1016/0165-1161(91)90250-C</identifier><identifier>PMID: 1899911</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Antineoplastic Agents - pharmacology ; Aroclor 1254 ; Aroclors - pharmacology ; Biological and medical sciences ; Cells, Cultured ; Chlorodiphenyl (54% Chlorine) ; Cultured human keratinocytes ; Cyclophosphamide ; Cyclophosphamide - pharmacology ; Cytochrome P450 ; Dose-Response Relationship, Drug ; Drug Synergism ; Drug toxicity and drugs side effects treatment ; Humans ; In Vitro Techniques ; keratinocytes ; Keratinocytes - drug effects ; Male ; man ; Medical sciences ; micronuclei ; Micronucleus Tests ; Micronucleus, in vitro ; Miscellaneous (drug allergy, mutagens, teratogens...) ; Mitomycin ; Mitomycin C ; Mitomycins - pharmacology ; Pharmacology. 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The cytokinesis-block method was applied to facilitate the scoring of micronucleated cells. Mitomycin C, a direct-acting compound, caused a 5–20-fold increase in micronuclei over the controls at the highest concentration tested (1 μg/ml). Cyclophosphamide, an agent requiring metabolic activation, did not induce the formation of micronuclei in cultured keratinocytes. However, after pretreatment of the keratinocyte cultures with Aroclor 1254 for 72 h, exposure to cyclophosphamide resulted in a 3-fold increase in micronucleus frequency over the controls. No cytogenetic effect of Aroclor 1254 was observed in control experiments.</description><subject>Antineoplastic Agents - pharmacology</subject><subject>Aroclor 1254</subject><subject>Aroclors - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Chlorodiphenyl (54% Chlorine)</subject><subject>Cultured human keratinocytes</subject><subject>Cyclophosphamide</subject><subject>Cyclophosphamide - pharmacology</subject><subject>Cytochrome P450</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Synergism</subject><subject>Drug toxicity and drugs side effects treatment</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>keratinocytes</subject><subject>Keratinocytes - drug effects</subject><subject>Male</subject><subject>man</subject><subject>Medical sciences</subject><subject>micronuclei</subject><subject>Micronucleus Tests</subject><subject>Micronucleus, in vitro</subject><subject>Miscellaneous (drug allergy, mutagens, teratogens...)</subject><subject>Mitomycin</subject><subject>Mitomycin C</subject><subject>Mitomycins - pharmacology</subject><subject>Pharmacology. 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Drug treatments</topic><topic>Time Factors</topic><toplevel>online_resources</toplevel><creatorcontrib>van Pelt, Frank N.A.M.</creatorcontrib><creatorcontrib>Haring, RenéM.</creatorcontrib><creatorcontrib>Overkamp, Miep J.I.</creatorcontrib><creatorcontrib>Weterings, Peter J.J.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Human Genome Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Mutation Research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van Pelt, Frank N.A.M.</au><au>Haring, RenéM.</au><au>Overkamp, Miep J.I.</au><au>Weterings, Peter J.J.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Micronucleus formation in cultured human keratinocytes following exposure to mitomycin C and cyclophosphamide</atitle><jtitle>Mutation Research</jtitle><addtitle>Mutat Res</addtitle><date>1991-02-01</date><risdate>1991</risdate><volume>252</volume><issue>1</issue><spage>45</spage><epage>50</epage><pages>45-50</pages><issn>0165-1161</issn><issn>0027-5107</issn><abstract>A method is described to investigate the induction of micronuclei in cultured human keratinocytes after short-term exposure to known clastogenic agents. The cytokinesis-block method was applied to facilitate the scoring of micronucleated cells. Mitomycin C, a direct-acting compound, caused a 5–20-fold increase in micronuclei over the controls at the highest concentration tested (1 μg/ml). Cyclophosphamide, an agent requiring metabolic activation, did not induce the formation of micronuclei in cultured keratinocytes. However, after pretreatment of the keratinocyte cultures with Aroclor 1254 for 72 h, exposure to cyclophosphamide resulted in a 3-fold increase in micronucleus frequency over the controls. No cytogenetic effect of Aroclor 1254 was observed in control experiments.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1899911</pmid><doi>10.1016/0165-1161(91)90250-C</doi><tpages>6</tpages></addata></record> |
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| ispartof | Mutation Research, 1991-02, Vol.252 (1), p.45-50 |
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| subjects | Antineoplastic Agents - pharmacology Aroclor 1254 Aroclors - pharmacology Biological and medical sciences Cells, Cultured Chlorodiphenyl (54% Chlorine) Cultured human keratinocytes Cyclophosphamide Cyclophosphamide - pharmacology Cytochrome P450 Dose-Response Relationship, Drug Drug Synergism Drug toxicity and drugs side effects treatment Humans In Vitro Techniques keratinocytes Keratinocytes - drug effects Male man Medical sciences micronuclei Micronucleus Tests Micronucleus, in vitro Miscellaneous (drug allergy, mutagens, teratogens...) Mitomycin Mitomycin C Mitomycins - pharmacology Pharmacology. Drug treatments Time Factors |
| title | Micronucleus formation in cultured human keratinocytes following exposure to mitomycin C and cyclophosphamide |
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