A simple and sensitive flow cytometric assay for the determination of the cytotoxic activity of human natural killer cells

A new, simple and sensitive flow cytometric assay for the determination of the cytotoxic activity of human natural killer cells is described. The assay is based on the use of two fluorochromes. The target cell population is stained with one fluorochrome (octadecylamine-fluorescein isothiocyanate, F-...

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Veröffentlicht in:	Journal of immunological methods 1990-12, Vol.135 (1), p.81-89
Hauptverfasser:	Radošević, Katarina, Garritsen, Henk S.P., Van Graft, Marja, De Grooth, Bart G., Greve, Jan
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Cell Line Chromium Radioisotopes Cytotoxicity Cytotoxicity Tests, Immunologic - methods Flow cytometry Flow Cytometry - methods Fluoresceins Fluorescent Dyes Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunobiology Immunological reactions in vitro Killer Cells, Natural - immunology Methods Natural killer Octadecylamine-fluorescein isothiocyanate Propidium Propidium iodide Sensitivity and Specificity
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