Carbosilane dendrimers inhibit α-synuclein fibrillation and prevent cells from rotenone-induced damage
[Display omitted] This study investigates the role of carbosilane dendrimers in fibrillation of α-synuclein and prevention of the mouse hippocampal cell (mHippoE-18) from rotenone-induced damage. Examining the interaction between carbosilane dendrimers and α-synuclein, we found that the dendrimers i...
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Veröffentlicht in: | International journal of pharmaceutics 2015-04, Vol.484 (1-2), p.268-275 |
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container_title | International journal of pharmaceutics |
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creator | Milowska, Katarzyna Szwed, Aleksandra Mutrynowska, Marta Gomez-Ramirez, Rafael de la Mata, Francisco Javier Gabryelak, Teresa Bryszewska, Maria |
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This study investigates the role of carbosilane dendrimers in fibrillation of α-synuclein and prevention of the mouse hippocampal cell (mHippoE-18) from rotenone-induced damage. Examining the interaction between carbosilane dendrimers and α-synuclein, we found that the dendrimers inhibit fibril formation. We also investigated cell viability, the production of reactive oxygen species (ROS), and mitochondrial membrane potential. mHippoE-18 cells were preincubated with carbosilane dendrimers before rotenone was added. All the dendrimers possess potential protection activity. Preincubation with dendrimers contributed to: increased viability, higher mitochondrial membrane potential, and reduced ROS level in cells. The probable mechanism of cell protection lies in the ability of dendrimers to capture rotenone by encapsulating or binding to its surface groups.
The fact that dendrimers have prevention potential is important in the search for new pharmacological strategies against neurodegenerative disorders. |
doi_str_mv | 10.1016/j.ijpharm.2015.02.066 |
format | Article |
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This study investigates the role of carbosilane dendrimers in fibrillation of α-synuclein and prevention of the mouse hippocampal cell (mHippoE-18) from rotenone-induced damage. Examining the interaction between carbosilane dendrimers and α-synuclein, we found that the dendrimers inhibit fibril formation. We also investigated cell viability, the production of reactive oxygen species (ROS), and mitochondrial membrane potential. mHippoE-18 cells were preincubated with carbosilane dendrimers before rotenone was added. All the dendrimers possess potential protection activity. Preincubation with dendrimers contributed to: increased viability, higher mitochondrial membrane potential, and reduced ROS level in cells. The probable mechanism of cell protection lies in the ability of dendrimers to capture rotenone by encapsulating or binding to its surface groups.
The fact that dendrimers have prevention potential is important in the search for new pharmacological strategies against neurodegenerative disorders.</description><identifier>ISSN: 0378-5173</identifier><identifier>EISSN: 1873-3476</identifier><identifier>DOI: 10.1016/j.ijpharm.2015.02.066</identifier><identifier>PMID: 25735664</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>alpha-Synuclein - antagonists & inhibitors ; alpha-Synuclein - metabolism ; Animals ; Carbosilane dendrimers ; Cell Line ; Cell Survival - drug effects ; Cell Survival - physiology ; Dendrimers - pharmacology ; Hippocampus - drug effects ; Hippocampus - metabolism ; Hippocampus - pathology ; Mice ; Parkinson’s disease ; Reactive Oxygen Species - metabolism ; Rotenone ; Rotenone - toxicity ; Silanes - pharmacology ; α-Synuclein</subject><ispartof>International journal of pharmaceutics, 2015-04, Vol.484 (1-2), p.268-275</ispartof><rights>2015 Elsevier B.V.</rights><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-a9c80283a3eb787df32e41a189b115df7739271e791326722b617d47791a63bf3</citedby><cites>FETCH-LOGICAL-c365t-a9c80283a3eb787df32e41a189b115df7739271e791326722b617d47791a63bf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijpharm.2015.02.066$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25735664$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Milowska, Katarzyna</creatorcontrib><creatorcontrib>Szwed, Aleksandra</creatorcontrib><creatorcontrib>Mutrynowska, Marta</creatorcontrib><creatorcontrib>Gomez-Ramirez, Rafael</creatorcontrib><creatorcontrib>de la Mata, Francisco Javier</creatorcontrib><creatorcontrib>Gabryelak, Teresa</creatorcontrib><creatorcontrib>Bryszewska, Maria</creatorcontrib><title>Carbosilane dendrimers inhibit α-synuclein fibrillation and prevent cells from rotenone-induced damage</title><title>International journal of pharmaceutics</title><addtitle>Int J Pharm</addtitle><description>[Display omitted]
This study investigates the role of carbosilane dendrimers in fibrillation of α-synuclein and prevention of the mouse hippocampal cell (mHippoE-18) from rotenone-induced damage. Examining the interaction between carbosilane dendrimers and α-synuclein, we found that the dendrimers inhibit fibril formation. We also investigated cell viability, the production of reactive oxygen species (ROS), and mitochondrial membrane potential. mHippoE-18 cells were preincubated with carbosilane dendrimers before rotenone was added. All the dendrimers possess potential protection activity. Preincubation with dendrimers contributed to: increased viability, higher mitochondrial membrane potential, and reduced ROS level in cells. The probable mechanism of cell protection lies in the ability of dendrimers to capture rotenone by encapsulating or binding to its surface groups.
The fact that dendrimers have prevention potential is important in the search for new pharmacological strategies against neurodegenerative disorders.</description><subject>alpha-Synuclein - antagonists & inhibitors</subject><subject>alpha-Synuclein - metabolism</subject><subject>Animals</subject><subject>Carbosilane dendrimers</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - physiology</subject><subject>Dendrimers - pharmacology</subject><subject>Hippocampus - drug effects</subject><subject>Hippocampus - metabolism</subject><subject>Hippocampus - pathology</subject><subject>Mice</subject><subject>Parkinson’s disease</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Rotenone</subject><subject>Rotenone - toxicity</subject><subject>Silanes - pharmacology</subject><subject>α-Synuclein</subject><issn>0378-5173</issn><issn>1873-3476</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1u2zAQhYkiRe24PUIDLrORyh-RlFZBYKRtAAPdtGuCEkc2DYl0SMmAj9WL9EyhYTfbrAYDvDdv3ofQV0pKSqj8ti_d_rAzcSwZoaIkrCRSfkBLWite8ErJG7QkXNWFoIov0G1Ke0KIZJR_QgsmFBdSVku0XZvYhuQG4wFb8Da6EWLCzu9c6yb872-RTn7uBnAe966NbhjM5ILHxlt8iHAEP-EOhiHhPoYRxzCBDx4K5-3cgcXWjGYLn9HH3gwJvlznCv35_vR7_bPY_PrxvH7cFB2XYipM09WE1dxwaFWtbM8ZVNTQumkpFbZXijdMUVAN5UwqxlpJla1U3o3kbc9X6P5y9xDDywxp0qNL5_dyvzAnTaUUVSMa3mSpuEi7GFKK0OtDLm_iSVOiz4z1Xl8Z6zNjTZjOjLPv7hoxtyPYN9d_qFnwcBFALnp0EHXqHPgMw0XoJm2DeyfiFT3FkbU</recordid><startdate>20150430</startdate><enddate>20150430</enddate><creator>Milowska, Katarzyna</creator><creator>Szwed, Aleksandra</creator><creator>Mutrynowska, Marta</creator><creator>Gomez-Ramirez, Rafael</creator><creator>de la Mata, Francisco Javier</creator><creator>Gabryelak, Teresa</creator><creator>Bryszewska, Maria</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150430</creationdate><title>Carbosilane dendrimers inhibit α-synuclein fibrillation and prevent cells from rotenone-induced damage</title><author>Milowska, Katarzyna ; Szwed, Aleksandra ; Mutrynowska, Marta ; Gomez-Ramirez, Rafael ; de la Mata, Francisco Javier ; Gabryelak, Teresa ; Bryszewska, Maria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-a9c80283a3eb787df32e41a189b115df7739271e791326722b617d47791a63bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>alpha-Synuclein - antagonists & inhibitors</topic><topic>alpha-Synuclein - metabolism</topic><topic>Animals</topic><topic>Carbosilane dendrimers</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - physiology</topic><topic>Dendrimers - pharmacology</topic><topic>Hippocampus - drug effects</topic><topic>Hippocampus - metabolism</topic><topic>Hippocampus - pathology</topic><topic>Mice</topic><topic>Parkinson’s disease</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Rotenone</topic><topic>Rotenone - toxicity</topic><topic>Silanes - pharmacology</topic><topic>α-Synuclein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Milowska, Katarzyna</creatorcontrib><creatorcontrib>Szwed, Aleksandra</creatorcontrib><creatorcontrib>Mutrynowska, Marta</creatorcontrib><creatorcontrib>Gomez-Ramirez, Rafael</creatorcontrib><creatorcontrib>de la Mata, Francisco Javier</creatorcontrib><creatorcontrib>Gabryelak, Teresa</creatorcontrib><creatorcontrib>Bryszewska, Maria</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of pharmaceutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Milowska, Katarzyna</au><au>Szwed, Aleksandra</au><au>Mutrynowska, Marta</au><au>Gomez-Ramirez, Rafael</au><au>de la Mata, Francisco Javier</au><au>Gabryelak, Teresa</au><au>Bryszewska, Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carbosilane dendrimers inhibit α-synuclein fibrillation and prevent cells from rotenone-induced damage</atitle><jtitle>International journal of pharmaceutics</jtitle><addtitle>Int J Pharm</addtitle><date>2015-04-30</date><risdate>2015</risdate><volume>484</volume><issue>1-2</issue><spage>268</spage><epage>275</epage><pages>268-275</pages><issn>0378-5173</issn><eissn>1873-3476</eissn><abstract>[Display omitted]
This study investigates the role of carbosilane dendrimers in fibrillation of α-synuclein and prevention of the mouse hippocampal cell (mHippoE-18) from rotenone-induced damage. Examining the interaction between carbosilane dendrimers and α-synuclein, we found that the dendrimers inhibit fibril formation. We also investigated cell viability, the production of reactive oxygen species (ROS), and mitochondrial membrane potential. mHippoE-18 cells were preincubated with carbosilane dendrimers before rotenone was added. All the dendrimers possess potential protection activity. Preincubation with dendrimers contributed to: increased viability, higher mitochondrial membrane potential, and reduced ROS level in cells. The probable mechanism of cell protection lies in the ability of dendrimers to capture rotenone by encapsulating or binding to its surface groups.
The fact that dendrimers have prevention potential is important in the search for new pharmacological strategies against neurodegenerative disorders.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>25735664</pmid><doi>10.1016/j.ijpharm.2015.02.066</doi><tpages>8</tpages></addata></record> |
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subjects | alpha-Synuclein - antagonists & inhibitors alpha-Synuclein - metabolism Animals Carbosilane dendrimers Cell Line Cell Survival - drug effects Cell Survival - physiology Dendrimers - pharmacology Hippocampus - drug effects Hippocampus - metabolism Hippocampus - pathology Mice Parkinson’s disease Reactive Oxygen Species - metabolism Rotenone Rotenone - toxicity Silanes - pharmacology α-Synuclein |
title | Carbosilane dendrimers inhibit α-synuclein fibrillation and prevent cells from rotenone-induced damage |
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