Mechanism of adenylate kinase. What can be learned from a mutant enzyme with minor perturbation in kinetic parameters?

The structural and functional roles of threonine-23 in the chicken muscle adenylate kinase (AK) were investigated by site-directed mutagenesis coupled with proton nuclear magnetic resonance (NMR) and phosphorus stereochemistry. The residue is potentially important because it is conserved among all t...

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Veröffentlicht in:	Biochemistry (Easton) 1993-06, Vol.32 (25), p.6450-6458
Hauptverfasser:	Shi, Zhengtao, Byeon, In Ja L, Jiang, Ru Tai, Tsai, Ming Daw
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenine Nucleotides - metabolism Adenylate Kinase - chemistry Adenylate Kinase - genetics Adenylate Kinase - metabolism Amino Acid Sequence Analytical, structural and metabolic biochemistry Animals Base Sequence Biological and medical sciences Chickens Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Guanidine Guanidines - pharmacology Hydrogen Bonding Kinetics Magnetic Resonance Spectroscopy - methods Models, Structural Molecular Sequence Data Muscles - enzymology Mutagenesis, Site-Directed Oligodeoxyribonucleotides Protein Conformation Protein Denaturation Protein Structure, Secondary Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Threonine Transferases
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