Chemical synthesis, molecular cloning, and expression of the gene coding for the Trichosanthes trypsin inhibitor--a squash family inhibitor

The gene coding for a Trichosanthes trypsin inhibitor analog (Ala-6-TTI) in which methionine at position 6 was replaced by alanine was synthesized chemically. The synthetic gene was cloned into plasmid pWR590-1 and expressed in Escherichia coli as a fusion protein composed of beta-galactosidase frag...

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Veröffentlicht in:	Journal of biochemistry (Tokyo) 1992, Vol.112 (1), p.45-51
Hauptverfasser:	Chen, X.M, Qian, Y.W, Chi, C.W, Gan, K.D, Zhang, M.F, Chen, C.Q
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acid sequences Base Sequence Biological and medical sciences Biotechnology cloning Cloning, Molecular Cyanogen Bromide enzyme activity Fundamental and applied biological sciences. Psychology gene expression Genes, Plant Genes, Synthetic Genetic Engineering Genetic technics genetic transformation Genetic Vectors Hydrolysis Methods. Procedures. Technologies Molecular Sequence Data Mutagenesis, Site-Directed nucleotide sequences Plant Proteins - chemical synthesis Plant Proteins - genetics Plant Proteins - isolation & purification Plant Proteins - metabolism Plasmids Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Saccharomyces cerevisiae - chemistry Saccharomyces cerevisiae - genetics synthesis Synthetic digonucleotides and genes. Sequencing synthetic genes Trichosanthes kirilowii trypsin trypsin inhibitors Trypsin Inhibitors - chemical synthesis Trypsin Inhibitors - genetics Trypsin Inhibitors - isolation & purification Trypsin Inhibitors - metabolism
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