Honokiol downregulates Kruppel-like factor 4 expression, attenuates inflammation, and reduces histopathology after spinal cord injury in rats
Randomized experimental study. To investigate the neuroprotective effect of honokiol (HNK) on rats subjected to traumatic spinal cord injury (SCI) and the molecular mechanisms. Inflammation contributes to the secondary injury to the spinal cord. Honokiol has been used as a neuroprotective agent beca...
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| Veröffentlicht in: | Spine (Philadelphia, Pa. 1976) Pa. 1976), 2015-03, Vol.40 (6), p.363-368 |
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| creator | Liu, Jia Zhang, Changmeng Liu, Zhi Zhang, Jianzheng Xiang, Zimin Sun, Tiansheng |
| description | Randomized experimental study.
To investigate the neuroprotective effect of honokiol (HNK) on rats subjected to traumatic spinal cord injury (SCI) and the molecular mechanisms.
Inflammation contributes to the secondary injury to the spinal cord. Honokiol has been used as a neuroprotective agent because of its strong antioxidant and anti-inflammatory properties. Kruppel-like factor 4 (Klf4) is a newly identified critical target for the anti-inflammatory effect of HNK. Whether HNK can inhibit inflammatory response in rat model of SCI through mediating the expression of Klf4 has yet to be elucidated.
Eighty-four adult female Sprague-Dawley rats were randomly divided into 4 groups as sham, SCI, SCI + Vehicle (0.1% propylene glycol in saline, intraperitoneally), and SCI + HNK (20 mg/kg, intraperitoneally) groups. The influences of HNK on the proinflammatory cytokines, microglial activation, neutrophil infiltration, histological changes, and improvement in motor function were assessed.
In the SCI group, proinflammatory cytokines, microglial activation, neutrophil infiltration, and Klf4 expression levels were increased compared with the sham group (P < 0.001). HNK intervention downregulated the expression of Klf4, reduced the production of proinflammatory cytokines, inhibited microglial activation, and neutrophil infiltration (P < 0.05). Furthermore, HNK also reduced histopathology and improved functional outcome after traumatic SCI.
HNK reduces secondary tissue damage and improves locomotor function recovery after SCI through suppressing inflammatory response, and can be used as a potential therapeutic agent for SCI.
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| doi_str_mv | 10.1097/BRS.0000000000000758 |
| format | Article |
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To investigate the neuroprotective effect of honokiol (HNK) on rats subjected to traumatic spinal cord injury (SCI) and the molecular mechanisms.
Inflammation contributes to the secondary injury to the spinal cord. Honokiol has been used as a neuroprotective agent because of its strong antioxidant and anti-inflammatory properties. Kruppel-like factor 4 (Klf4) is a newly identified critical target for the anti-inflammatory effect of HNK. Whether HNK can inhibit inflammatory response in rat model of SCI through mediating the expression of Klf4 has yet to be elucidated.
Eighty-four adult female Sprague-Dawley rats were randomly divided into 4 groups as sham, SCI, SCI + Vehicle (0.1% propylene glycol in saline, intraperitoneally), and SCI + HNK (20 mg/kg, intraperitoneally) groups. The influences of HNK on the proinflammatory cytokines, microglial activation, neutrophil infiltration, histological changes, and improvement in motor function were assessed.
In the SCI group, proinflammatory cytokines, microglial activation, neutrophil infiltration, and Klf4 expression levels were increased compared with the sham group (P < 0.001). HNK intervention downregulated the expression of Klf4, reduced the production of proinflammatory cytokines, inhibited microglial activation, and neutrophil infiltration (P < 0.05). Furthermore, HNK also reduced histopathology and improved functional outcome after traumatic SCI.
HNK reduces secondary tissue damage and improves locomotor function recovery after SCI through suppressing inflammatory response, and can be used as a potential therapeutic agent for SCI.
NA.</description><identifier>ISSN: 0362-2436</identifier><identifier>EISSN: 1528-1159</identifier><identifier>DOI: 10.1097/BRS.0000000000000758</identifier><identifier>PMID: 25774462</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Antioxidants - metabolism ; Biphenyl Compounds - pharmacology ; Cytokines - metabolism ; Down-Regulation - drug effects ; Female ; Inflammation - drug therapy ; Inflammation - pathology ; Kruppel-Like Transcription Factors - analysis ; Kruppel-Like Transcription Factors - metabolism ; Lignans - pharmacology ; Neuroprotective Agents - pharmacology ; Rats ; Rats, Sprague-Dawley ; Spinal Cord - drug effects ; Spinal Cord - pathology ; Spinal Cord Injuries - drug therapy ; Spinal Cord Injuries - metabolism ; Spinal Cord Injuries - pathology</subject><ispartof>Spine (Philadelphia, Pa. 1976), 2015-03, Vol.40 (6), p.363-368</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c307t-6385f5b25e5aab704f682e4ac564a8c518ad3aa387d15f2c65a6228c562dc99b3</citedby><cites>FETCH-LOGICAL-c307t-6385f5b25e5aab704f682e4ac564a8c518ad3aa387d15f2c65a6228c562dc99b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25774462$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Jia</creatorcontrib><creatorcontrib>Zhang, Changmeng</creatorcontrib><creatorcontrib>Liu, Zhi</creatorcontrib><creatorcontrib>Zhang, Jianzheng</creatorcontrib><creatorcontrib>Xiang, Zimin</creatorcontrib><creatorcontrib>Sun, Tiansheng</creatorcontrib><title>Honokiol downregulates Kruppel-like factor 4 expression, attenuates inflammation, and reduces histopathology after spinal cord injury in rats</title><title>Spine (Philadelphia, Pa. 1976)</title><addtitle>Spine (Phila Pa 1976)</addtitle><description>Randomized experimental study.
To investigate the neuroprotective effect of honokiol (HNK) on rats subjected to traumatic spinal cord injury (SCI) and the molecular mechanisms.
Inflammation contributes to the secondary injury to the spinal cord. Honokiol has been used as a neuroprotective agent because of its strong antioxidant and anti-inflammatory properties. Kruppel-like factor 4 (Klf4) is a newly identified critical target for the anti-inflammatory effect of HNK. Whether HNK can inhibit inflammatory response in rat model of SCI through mediating the expression of Klf4 has yet to be elucidated.
Eighty-four adult female Sprague-Dawley rats were randomly divided into 4 groups as sham, SCI, SCI + Vehicle (0.1% propylene glycol in saline, intraperitoneally), and SCI + HNK (20 mg/kg, intraperitoneally) groups. The influences of HNK on the proinflammatory cytokines, microglial activation, neutrophil infiltration, histological changes, and improvement in motor function were assessed.
In the SCI group, proinflammatory cytokines, microglial activation, neutrophil infiltration, and Klf4 expression levels were increased compared with the sham group (P < 0.001). HNK intervention downregulated the expression of Klf4, reduced the production of proinflammatory cytokines, inhibited microglial activation, and neutrophil infiltration (P < 0.05). Furthermore, HNK also reduced histopathology and improved functional outcome after traumatic SCI.
HNK reduces secondary tissue damage and improves locomotor function recovery after SCI through suppressing inflammatory response, and can be used as a potential therapeutic agent for SCI.
NA.</description><subject>Animals</subject><subject>Antioxidants - metabolism</subject><subject>Biphenyl Compounds - pharmacology</subject><subject>Cytokines - metabolism</subject><subject>Down-Regulation - drug effects</subject><subject>Female</subject><subject>Inflammation - drug therapy</subject><subject>Inflammation - pathology</subject><subject>Kruppel-Like Transcription Factors - analysis</subject><subject>Kruppel-Like Transcription Factors - metabolism</subject><subject>Lignans - pharmacology</subject><subject>Neuroprotective Agents - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Spinal Cord - drug effects</subject><subject>Spinal Cord - pathology</subject><subject>Spinal Cord Injuries - drug therapy</subject><subject>Spinal Cord Injuries - metabolism</subject><subject>Spinal Cord Injuries - pathology</subject><issn>0362-2436</issn><issn>1528-1159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1O3EAQhEdRELv8vEGE5pgDXjy_to9kBQGBhETC2eodt8Hs2GNmxgr7ELxzhiwglL7Uob7qlroI-cbyBcur4uTH7a9F_nkKVX4hc6Z4mTGmqq9kngvNMy6FnpG9EB4TowWrdsmMq6KQUvM5eblwg1t3ztLG_Rk83k8WIgZ65adxRJvZbo20BROdp5Li8-gxhM4NxxRixGH6B3dDa6HvIW6NoaEem8kk56EL0Y0QH5x19xsKbURPw9gNYKlxvknRx8lvklAPMRyQnRZswMM33Sd352e_lxfZ9c3Py-XpdWZEXsRMi1K1asUVKoBVkctWlxwlGKUllEaxEhoBIMqiYarlRivQnCdD88ZU1Ursk-_bvaN3TxOGWPddMGgtDOimUDOtZRqdy4TKLWq8C8FjW4--68FvapbXr0XUqYj6_yJS7OjtwrTqsfkIvX9e_AUDTobx</recordid><startdate>20150315</startdate><enddate>20150315</enddate><creator>Liu, Jia</creator><creator>Zhang, Changmeng</creator><creator>Liu, Zhi</creator><creator>Zhang, Jianzheng</creator><creator>Xiang, Zimin</creator><creator>Sun, Tiansheng</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150315</creationdate><title>Honokiol downregulates Kruppel-like factor 4 expression, attenuates inflammation, and reduces histopathology after spinal cord injury in rats</title><author>Liu, Jia ; Zhang, Changmeng ; Liu, Zhi ; Zhang, Jianzheng ; Xiang, Zimin ; Sun, Tiansheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c307t-6385f5b25e5aab704f682e4ac564a8c518ad3aa387d15f2c65a6228c562dc99b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Antioxidants - metabolism</topic><topic>Biphenyl Compounds - pharmacology</topic><topic>Cytokines - metabolism</topic><topic>Down-Regulation - drug effects</topic><topic>Female</topic><topic>Inflammation - drug therapy</topic><topic>Inflammation - pathology</topic><topic>Kruppel-Like Transcription Factors - analysis</topic><topic>Kruppel-Like Transcription Factors - metabolism</topic><topic>Lignans - pharmacology</topic><topic>Neuroprotective Agents - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Spinal Cord - drug effects</topic><topic>Spinal Cord - pathology</topic><topic>Spinal Cord Injuries - drug therapy</topic><topic>Spinal Cord Injuries - metabolism</topic><topic>Spinal Cord Injuries - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Jia</creatorcontrib><creatorcontrib>Zhang, Changmeng</creatorcontrib><creatorcontrib>Liu, Zhi</creatorcontrib><creatorcontrib>Zhang, Jianzheng</creatorcontrib><creatorcontrib>Xiang, Zimin</creatorcontrib><creatorcontrib>Sun, Tiansheng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Spine (Philadelphia, Pa. 1976)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Jia</au><au>Zhang, Changmeng</au><au>Liu, Zhi</au><au>Zhang, Jianzheng</au><au>Xiang, Zimin</au><au>Sun, Tiansheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Honokiol downregulates Kruppel-like factor 4 expression, attenuates inflammation, and reduces histopathology after spinal cord injury in rats</atitle><jtitle>Spine (Philadelphia, Pa. 1976)</jtitle><addtitle>Spine (Phila Pa 1976)</addtitle><date>2015-03-15</date><risdate>2015</risdate><volume>40</volume><issue>6</issue><spage>363</spage><epage>368</epage><pages>363-368</pages><issn>0362-2436</issn><eissn>1528-1159</eissn><abstract>Randomized experimental study.
To investigate the neuroprotective effect of honokiol (HNK) on rats subjected to traumatic spinal cord injury (SCI) and the molecular mechanisms.
Inflammation contributes to the secondary injury to the spinal cord. Honokiol has been used as a neuroprotective agent because of its strong antioxidant and anti-inflammatory properties. Kruppel-like factor 4 (Klf4) is a newly identified critical target for the anti-inflammatory effect of HNK. Whether HNK can inhibit inflammatory response in rat model of SCI through mediating the expression of Klf4 has yet to be elucidated.
Eighty-four adult female Sprague-Dawley rats were randomly divided into 4 groups as sham, SCI, SCI + Vehicle (0.1% propylene glycol in saline, intraperitoneally), and SCI + HNK (20 mg/kg, intraperitoneally) groups. The influences of HNK on the proinflammatory cytokines, microglial activation, neutrophil infiltration, histological changes, and improvement in motor function were assessed.
In the SCI group, proinflammatory cytokines, microglial activation, neutrophil infiltration, and Klf4 expression levels were increased compared with the sham group (P < 0.001). HNK intervention downregulated the expression of Klf4, reduced the production of proinflammatory cytokines, inhibited microglial activation, and neutrophil infiltration (P < 0.05). Furthermore, HNK also reduced histopathology and improved functional outcome after traumatic SCI.
HNK reduces secondary tissue damage and improves locomotor function recovery after SCI through suppressing inflammatory response, and can be used as a potential therapeutic agent for SCI.
NA.</abstract><cop>United States</cop><pmid>25774462</pmid><doi>10.1097/BRS.0000000000000758</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Antioxidants - metabolism Biphenyl Compounds - pharmacology Cytokines - metabolism Down-Regulation - drug effects Female Inflammation - drug therapy Inflammation - pathology Kruppel-Like Transcription Factors - analysis Kruppel-Like Transcription Factors - metabolism Lignans - pharmacology Neuroprotective Agents - pharmacology Rats Rats, Sprague-Dawley Spinal Cord - drug effects Spinal Cord - pathology Spinal Cord Injuries - drug therapy Spinal Cord Injuries - metabolism Spinal Cord Injuries - pathology |
| title | Honokiol downregulates Kruppel-like factor 4 expression, attenuates inflammation, and reduces histopathology after spinal cord injury in rats |
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