Pulchinenoside inhibits the fibroblast-like synoviocytes apoptosis in adjuvant arthritis rats
To explore the eff ect of pulchinenoside (PULC) on fi broblast-like synoviocytes (FLS) apoptosis in adjuvant arthritis (AA) rats. A total of 60 SD rats were randomly divided into 8 groups: A normal control group, an AA group, a low PULC group (50 mg/kg), a middle PULC group (100 mg/kg) or a high PUL...
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| Veröffentlicht in: | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban 2015-02, Vol.40 (2), p.144-149 |
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| container_title | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban |
| container_volume | 40 |
| creator | Miao, Chenggui Zhou, Guoliang Qin, Meisong Chen, Jianzhong Li, Chengfeng He, Huaqi |
| description | To explore the eff ect of pulchinenoside (PULC) on fi broblast-like synoviocytes (FLS) apoptosis in adjuvant arthritis (AA) rats.
A total of 60 SD rats were randomly divided into 8 groups: A normal control group, an AA group, a low PULC group (50 mg/kg), a middle PULC group (100 mg/kg) or a high PULC group (150 mg/kg) and an ibuprofen (8 mg/kg) group (n=10 per group). FLS from the AA rats was cultured. The expression of Bcl-2, Bax, caspase-3 and the FLS proliferation were detected by the real time qPCR and MTT, respectively. The expression of IL-6 and IL-8 in culture medium was detected by ELISA.
Compared with the AA group, the Bcl-2 expression was down-regulated (all P |
| doi_str_mv | 10.11817/j.issn.1672-7347.2015.02.005 |
| format | Article |
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A total of 60 SD rats were randomly divided into 8 groups: A normal control group, an AA group, a low PULC group (50 mg/kg), a middle PULC group (100 mg/kg) or a high PULC group (150 mg/kg) and an ibuprofen (8 mg/kg) group (n=10 per group). FLS from the AA rats was cultured. The expression of Bcl-2, Bax, caspase-3 and the FLS proliferation were detected by the real time qPCR and MTT, respectively. The expression of IL-6 and IL-8 in culture medium was detected by ELISA.
Compared with the AA group, the Bcl-2 expression was down-regulated (all P<0.05), the Bax and caspase-3 expression was up-regulated (all P<0.05), and the FLS proliferation was inhibited (all P<0.05). The IL-6 and IL-8 expression was suppressed in the FLS in the PULC groups at different dosages (all P<0.05) as well as in the ibuprofen group (P<0.05).
PULC may inhibit the FLS proliferation in AA rats by increase in FLS</description><identifier>ISSN: 1672-7347</identifier><identifier>DOI: 10.11817/j.issn.1672-7347.2015.02.005</identifier><identifier>PMID: 25769322</identifier><language>chi</language><publisher>China</publisher><subject>Animals ; Apoptosis - drug effects ; Arthritis, Experimental ; bcl-2-Associated X Protein - metabolism ; Caspase 3 - metabolism ; Fibroblasts - cytology ; Fibroblasts - drug effects ; Interleukin-6 - metabolism ; Interleukin-8 - metabolism ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Pulsatilla - chemistry ; Rats ; Rats, Sprague-Dawley ; Synovial Membrane - cytology</subject><ispartof>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban, 2015-02, Vol.40 (2), p.144-149</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27933,27934</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25769322$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miao, Chenggui</creatorcontrib><creatorcontrib>Zhou, Guoliang</creatorcontrib><creatorcontrib>Qin, Meisong</creatorcontrib><creatorcontrib>Chen, Jianzhong</creatorcontrib><creatorcontrib>Li, Chengfeng</creatorcontrib><creatorcontrib>He, Huaqi</creatorcontrib><title>Pulchinenoside inhibits the fibroblast-like synoviocytes apoptosis in adjuvant arthritis rats</title><title>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban</title><addtitle>Zhong Nan Da Xue Xue Bao Yi Xue Ban</addtitle><description>To explore the eff ect of pulchinenoside (PULC) on fi broblast-like synoviocytes (FLS) apoptosis in adjuvant arthritis (AA) rats.
A total of 60 SD rats were randomly divided into 8 groups: A normal control group, an AA group, a low PULC group (50 mg/kg), a middle PULC group (100 mg/kg) or a high PULC group (150 mg/kg) and an ibuprofen (8 mg/kg) group (n=10 per group). FLS from the AA rats was cultured. The expression of Bcl-2, Bax, caspase-3 and the FLS proliferation were detected by the real time qPCR and MTT, respectively. The expression of IL-6 and IL-8 in culture medium was detected by ELISA.
Compared with the AA group, the Bcl-2 expression was down-regulated (all P<0.05), the Bax and caspase-3 expression was up-regulated (all P<0.05), and the FLS proliferation was inhibited (all P<0.05). The IL-6 and IL-8 expression was suppressed in the FLS in the PULC groups at different dosages (all P<0.05) as well as in the ibuprofen group (P<0.05).
PULC may inhibit the FLS proliferation in AA rats by increase in FLS</description><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Arthritis, Experimental</subject><subject>bcl-2-Associated X Protein - metabolism</subject><subject>Caspase 3 - metabolism</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - drug effects</subject><subject>Interleukin-6 - metabolism</subject><subject>Interleukin-8 - metabolism</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Pulsatilla - chemistry</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Synovial Membrane - cytology</subject><issn>1672-7347</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE9LwzAchnNQ3Jj7CpKL4KU1f9qkOcpQJwz0oEcpSZrQzK6t-aWDfXsLTk8vvDzPe3gRuqUkp7Si8n6fB4A-p0KyTPJC5ozQMicsJ6S8QMv_foHWAMEQopTiopJXaMFKKRRnbIk-36bOtqF3_QChcTj0bTAhAU6twz6YOJhOQ8q68OUwnPrhGAZ7Sg6wHocxzRLMDtbNfjrqPmEdUxtDmtuoE1yjS687cOtzrtDH0-P7ZpvtXp9fNg-7bKRMpIxLb5QopXTWNp5UpS4ao0nhqVS8aozwlquq4JWRQhjDBLXCekEIL0rHZmaF7n53xzh8Tw5SfQhgXdfp3g0T1FSIglHJOZ3RmzM6mYNr6jGGg46n-u8S_gOX7Gep</recordid><startdate>201502</startdate><enddate>201502</enddate><creator>Miao, Chenggui</creator><creator>Zhou, Guoliang</creator><creator>Qin, Meisong</creator><creator>Chen, Jianzhong</creator><creator>Li, Chengfeng</creator><creator>He, Huaqi</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201502</creationdate><title>Pulchinenoside inhibits the fibroblast-like synoviocytes apoptosis in adjuvant arthritis rats</title><author>Miao, Chenggui ; Zhou, Guoliang ; Qin, Meisong ; Chen, Jianzhong ; Li, Chengfeng ; He, Huaqi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-37fb96577eccdf085a4dba04f17938db6fc398438b766bb261c6cf600345e2793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>Arthritis, Experimental</topic><topic>bcl-2-Associated X Protein - metabolism</topic><topic>Caspase 3 - metabolism</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - drug effects</topic><topic>Interleukin-6 - metabolism</topic><topic>Interleukin-8 - metabolism</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Pulsatilla - chemistry</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Synovial Membrane - cytology</topic><toplevel>online_resources</toplevel><creatorcontrib>Miao, Chenggui</creatorcontrib><creatorcontrib>Zhou, Guoliang</creatorcontrib><creatorcontrib>Qin, Meisong</creatorcontrib><creatorcontrib>Chen, Jianzhong</creatorcontrib><creatorcontrib>Li, Chengfeng</creatorcontrib><creatorcontrib>He, Huaqi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miao, Chenggui</au><au>Zhou, Guoliang</au><au>Qin, Meisong</au><au>Chen, Jianzhong</au><au>Li, Chengfeng</au><au>He, Huaqi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pulchinenoside inhibits the fibroblast-like synoviocytes apoptosis in adjuvant arthritis rats</atitle><jtitle>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban</jtitle><addtitle>Zhong Nan Da Xue Xue Bao Yi Xue Ban</addtitle><date>2015-02</date><risdate>2015</risdate><volume>40</volume><issue>2</issue><spage>144</spage><epage>149</epage><pages>144-149</pages><issn>1672-7347</issn><abstract>To explore the eff ect of pulchinenoside (PULC) on fi broblast-like synoviocytes (FLS) apoptosis in adjuvant arthritis (AA) rats.
A total of 60 SD rats were randomly divided into 8 groups: A normal control group, an AA group, a low PULC group (50 mg/kg), a middle PULC group (100 mg/kg) or a high PULC group (150 mg/kg) and an ibuprofen (8 mg/kg) group (n=10 per group). FLS from the AA rats was cultured. The expression of Bcl-2, Bax, caspase-3 and the FLS proliferation were detected by the real time qPCR and MTT, respectively. The expression of IL-6 and IL-8 in culture medium was detected by ELISA.
Compared with the AA group, the Bcl-2 expression was down-regulated (all P<0.05), the Bax and caspase-3 expression was up-regulated (all P<0.05), and the FLS proliferation was inhibited (all P<0.05). The IL-6 and IL-8 expression was suppressed in the FLS in the PULC groups at different dosages (all P<0.05) as well as in the ibuprofen group (P<0.05).
PULC may inhibit the FLS proliferation in AA rats by increase in FLS</abstract><cop>China</cop><pmid>25769322</pmid><doi>10.11817/j.issn.1672-7347.2015.02.005</doi><tpages>6</tpages></addata></record> |
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| ispartof | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban, 2015-02, Vol.40 (2), p.144-149 |
| issn | 1672-7347 |
| language | chi |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Apoptosis - drug effects Arthritis, Experimental bcl-2-Associated X Protein - metabolism Caspase 3 - metabolism Fibroblasts - cytology Fibroblasts - drug effects Interleukin-6 - metabolism Interleukin-8 - metabolism Proto-Oncogene Proteins c-bcl-2 - metabolism Pulsatilla - chemistry Rats Rats, Sprague-Dawley Synovial Membrane - cytology |
| title | Pulchinenoside inhibits the fibroblast-like synoviocytes apoptosis in adjuvant arthritis rats |
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