Acclimation of nitrifying biomass and its effect on 2-chlorophenol removal
The metabolic and kinetic behavior of a nitrifying sludge exposed to 2-chlorophenol (2-CP) was evaluated in batch cultures. Two kinds of nitrifying culture were used; one acclimated to 4-methylphenol (4-mp), and the other unacclimated to 4-mp. The unacclimated culture was affected adversely by the 2...
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| Veröffentlicht in: | Water science and technology 2015, Vol.71 (2), p.277-282 |
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| creator | Pérez-Alfaro, J E González-Blanco, G Sierra-Palacios, E Marcial-Quino, J Beristain-Cardoso, R |
| description | The metabolic and kinetic behavior of a nitrifying sludge exposed to 2-chlorophenol (2-CP) was evaluated in batch cultures. Two kinds of nitrifying culture were used; one acclimated to 4-methylphenol (4-mp), and the other unacclimated to 4-mp. The unacclimated culture was affected adversely by the 2-CP's presence, since neither nitrification nor 2-CP oxidation was observed. Nonetheless, the acclimated culture showed metabolic capacity to nitrify and mineralize 2-CP. Ammonium removal was 100%, with a nitrifying yield of 0.92 ± 0.04 mg NO(3)(-)-N/mg NH(4)(+)-N consumed. The consumption efficiency for 2-CP was 100% and the halogenated compound was mineralized to CO2. Denaturing gradient gel electrophoresis (DGGE) patterns showed the shift in microbial community structure, indicating that microbial diversity was due to the acclimation process. This is the first evidence where nitrifying culture acclimated to 4-mp completely removed ammonium and 2-CP. |
| doi_str_mv | 10.2166/wst.2014.508 |
| format | Article |
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Two kinds of nitrifying culture were used; one acclimated to 4-methylphenol (4-mp), and the other unacclimated to 4-mp. The unacclimated culture was affected adversely by the 2-CP's presence, since neither nitrification nor 2-CP oxidation was observed. Nonetheless, the acclimated culture showed metabolic capacity to nitrify and mineralize 2-CP. Ammonium removal was 100%, with a nitrifying yield of 0.92 ± 0.04 mg NO(3)(-)-N/mg NH(4)(+)-N consumed. The consumption efficiency for 2-CP was 100% and the halogenated compound was mineralized to CO2. Denaturing gradient gel electrophoresis (DGGE) patterns showed the shift in microbial community structure, indicating that microbial diversity was due to the acclimation process. This is the first evidence where nitrifying culture acclimated to 4-mp completely removed ammonium and 2-CP.</description><identifier>ISSN: 0273-1223</identifier><identifier>EISSN: 1996-9732</identifier><identifier>DOI: 10.2166/wst.2014.508</identifier><identifier>PMID: 25633952</identifier><language>eng</language><publisher>England: IWA Publishing</publisher><subject>Acclimation ; Acclimatization ; Ammonium ; Ammonium compounds ; Ammonium Compounds - metabolism ; Analytical methods ; Bacteria - genetics ; Bacteria - growth & development ; Bacteria - isolation & purification ; Bacteria - metabolism ; Biodegradation ; Biodegradation, Environmental ; Biofilms ; Capacity ; Carbon dioxide ; Chlorophenol ; Chlorophenols - metabolism ; Community structure ; Culture ; Deoxyribonucleic acid ; DNA ; Ecosystems ; Fatty acids ; Gel electrophoresis ; Gels ; Kinetics ; Microbiology ; Microorganisms ; Nitrates ; Nitrification ; Oxidation ; Oxidation-Reduction ; Phenols ; Pollutants ; Proteins ; Removal ; Sewage - chemistry ; Sewage - microbiology ; Sludge ; Startups ; Toxicity</subject><ispartof>Water science and technology, 2015, Vol.71 (2), p.277-282</ispartof><rights>Copyright IWA Publishing Jan 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-3b32e1dc8202518e90df734a0987a2b4ce81c54979caf9ccfd6b7b3c4466c4203</citedby><cites>FETCH-LOGICAL-c352t-3b32e1dc8202518e90df734a0987a2b4ce81c54979caf9ccfd6b7b3c4466c4203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,4025,27928,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25633952$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pérez-Alfaro, J E</creatorcontrib><creatorcontrib>González-Blanco, G</creatorcontrib><creatorcontrib>Sierra-Palacios, E</creatorcontrib><creatorcontrib>Marcial-Quino, J</creatorcontrib><creatorcontrib>Beristain-Cardoso, R</creatorcontrib><title>Acclimation of nitrifying biomass and its effect on 2-chlorophenol removal</title><title>Water science and technology</title><addtitle>Water Sci Technol</addtitle><description>The metabolic and kinetic behavior of a nitrifying sludge exposed to 2-chlorophenol (2-CP) was evaluated in batch cultures. Two kinds of nitrifying culture were used; one acclimated to 4-methylphenol (4-mp), and the other unacclimated to 4-mp. The unacclimated culture was affected adversely by the 2-CP's presence, since neither nitrification nor 2-CP oxidation was observed. Nonetheless, the acclimated culture showed metabolic capacity to nitrify and mineralize 2-CP. Ammonium removal was 100%, with a nitrifying yield of 0.92 ± 0.04 mg NO(3)(-)-N/mg NH(4)(+)-N consumed. The consumption efficiency for 2-CP was 100% and the halogenated compound was mineralized to CO2. Denaturing gradient gel electrophoresis (DGGE) patterns showed the shift in microbial community structure, indicating that microbial diversity was due to the acclimation process. This is the first evidence where nitrifying culture acclimated to 4-mp completely removed ammonium and 2-CP.</description><subject>Acclimation</subject><subject>Acclimatization</subject><subject>Ammonium</subject><subject>Ammonium compounds</subject><subject>Ammonium Compounds - metabolism</subject><subject>Analytical methods</subject><subject>Bacteria - genetics</subject><subject>Bacteria - growth & development</subject><subject>Bacteria - isolation & purification</subject><subject>Bacteria - metabolism</subject><subject>Biodegradation</subject><subject>Biodegradation, Environmental</subject><subject>Biofilms</subject><subject>Capacity</subject><subject>Carbon dioxide</subject><subject>Chlorophenol</subject><subject>Chlorophenols - metabolism</subject><subject>Community structure</subject><subject>Culture</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Ecosystems</subject><subject>Fatty acids</subject><subject>Gel electrophoresis</subject><subject>Gels</subject><subject>Kinetics</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Nitrates</subject><subject>Nitrification</subject><subject>Oxidation</subject><subject>Oxidation-Reduction</subject><subject>Phenols</subject><subject>Pollutants</subject><subject>Proteins</subject><subject>Removal</subject><subject>Sewage - chemistry</subject><subject>Sewage - microbiology</subject><subject>Sludge</subject><subject>Startups</subject><subject>Toxicity</subject><issn>0273-1223</issn><issn>1996-9732</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqNkTtPwzAURi0EoqWwMaNILAyk2NeOE4-o4qlKLDBbjmNTV0lc7BTUf4-rFgYmJi9Hn67PQeic4CkQzm--4jAFTNi0wNUBGhMheC5KCodojKGkOQGgI3QS4xJjXFKGj9EICk6pKGCMnm-1bl2nBuf7zNusd0NwduP696x2vlMxZqpvMjfEzFhr9JAlDnK9aH3wq4XpfZsF0_lP1Z6iI6vaaM727wS93d-9zh7z-cvD0-x2nmtawJDTmoIhja4AQ0EqI3Bj01kKi6pUUDNtKqILJkqhlRVa24bXZU01Y5xrBphO0NVudxX8x9rEQXYuatO2qjd-HWWSwoAQSsQ_0AIYw7RgCb38gy79OvTpIzINYVFynqxO0PWO0sHHGIyVq5DshY0kWG5zyJRDbnPIlCPhF_vRdd2Z5hf-8U-_AYtrhDY</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>Pérez-Alfaro, J E</creator><creator>González-Blanco, G</creator><creator>Sierra-Palacios, E</creator><creator>Marcial-Quino, J</creator><creator>Beristain-Cardoso, R</creator><general>IWA Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QH</scope><scope>7UA</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H96</scope><scope>H97</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>L6V</scope><scope>M0S</scope><scope>M1P</scope><scope>M7S</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>7X8</scope><scope>7ST</scope><scope>SOI</scope></search><sort><creationdate>2015</creationdate><title>Acclimation of nitrifying biomass and its effect on 2-chlorophenol removal</title><author>Pérez-Alfaro, J E ; González-Blanco, G ; Sierra-Palacios, E ; Marcial-Quino, J ; Beristain-Cardoso, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-3b32e1dc8202518e90df734a0987a2b4ce81c54979caf9ccfd6b7b3c4466c4203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acclimation</topic><topic>Acclimatization</topic><topic>Ammonium</topic><topic>Ammonium compounds</topic><topic>Ammonium Compounds - 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Academic</collection><collection>Environment Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Water science and technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pérez-Alfaro, J E</au><au>González-Blanco, G</au><au>Sierra-Palacios, E</au><au>Marcial-Quino, J</au><au>Beristain-Cardoso, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acclimation of nitrifying biomass and its effect on 2-chlorophenol removal</atitle><jtitle>Water science and technology</jtitle><addtitle>Water Sci Technol</addtitle><date>2015</date><risdate>2015</risdate><volume>71</volume><issue>2</issue><spage>277</spage><epage>282</epage><pages>277-282</pages><issn>0273-1223</issn><eissn>1996-9732</eissn><abstract>The metabolic and kinetic behavior of a nitrifying sludge exposed to 2-chlorophenol (2-CP) was evaluated in batch cultures. Two kinds of nitrifying culture were used; one acclimated to 4-methylphenol (4-mp), and the other unacclimated to 4-mp. The unacclimated culture was affected adversely by the 2-CP's presence, since neither nitrification nor 2-CP oxidation was observed. Nonetheless, the acclimated culture showed metabolic capacity to nitrify and mineralize 2-CP. Ammonium removal was 100%, with a nitrifying yield of 0.92 ± 0.04 mg NO(3)(-)-N/mg NH(4)(+)-N consumed. The consumption efficiency for 2-CP was 100% and the halogenated compound was mineralized to CO2. Denaturing gradient gel electrophoresis (DGGE) patterns showed the shift in microbial community structure, indicating that microbial diversity was due to the acclimation process. This is the first evidence where nitrifying culture acclimated to 4-mp completely removed ammonium and 2-CP.</abstract><cop>England</cop><pub>IWA Publishing</pub><pmid>25633952</pmid><doi>10.2166/wst.2014.508</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Water science and technology, 2015, Vol.71 (2), p.277-282 |
| issn | 0273-1223 1996-9732 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Acclimation Acclimatization Ammonium Ammonium compounds Ammonium Compounds - metabolism Analytical methods Bacteria - genetics Bacteria - growth & development Bacteria - isolation & purification Bacteria - metabolism Biodegradation Biodegradation, Environmental Biofilms Capacity Carbon dioxide Chlorophenol Chlorophenols - metabolism Community structure Culture Deoxyribonucleic acid DNA Ecosystems Fatty acids Gel electrophoresis Gels Kinetics Microbiology Microorganisms Nitrates Nitrification Oxidation Oxidation-Reduction Phenols Pollutants Proteins Removal Sewage - chemistry Sewage - microbiology Sludge Startups Toxicity |
| title | Acclimation of nitrifying biomass and its effect on 2-chlorophenol removal |
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