Assessment of the effect of triton X-114 on the physicochemical properties of an antibody fragment

The effect of Triton X‐114 on the physicochemical properties of a single‐chain antibody fragment (scFv) has been studied. According to the far UV circular dichroism spectroscopy, the secondary structure of the recombinant antibody was not significantly affected by the presence of Triton. From the an...

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Veröffentlicht in:	Biotechnology progress 2014-05, Vol.30 (3), p.554-561
Hauptverfasser:	Malpiedi, Luciana P., Nerli, Bibiana B., Abdalla, Dulcineia S. P., Pessoa Jr, Adalberto
Format:	Artikel
Sprache:	eng
Schlagworte:	Circular Dichroism Immunoglobulin Fragments - chemistry Immunoglobulin Fragments - immunology Immunoglobulin Fragments - isolation & purification Immunoglobulin Variable Region - chemistry Liquid-Liquid Extraction nonionic surfactant Polyethylene Glycols - chemistry Polyethylene Glycols - pharmacology Protein Stability Protein Structure, Secondary Recombinant Proteins - chemistry Recombinant Proteins - immunology Recombinant Proteins - isolation & purification single-chain antibody fragment spectroscopic study Surface-Active Agents
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