Comparative profiling of the sperm proteome
The highly complex and species‐selective mechanism of fertilization is a central theme of developmental biology. Gametogenesis, sperm activation, and egg–sperm recognition are fundamental biological processes, warranting detailed studies into the molecular composition of gametes. Biological MS has b...
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| Veröffentlicht in: | Proteomics (Weinheim) 2015-02, Vol.15 (4), p.632-648 |
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| creator | Holland, Ashling Ohlendieck, Kay |
| description | The highly complex and species‐selective mechanism of fertilization is a central theme of developmental biology. Gametogenesis, sperm activation, and egg–sperm recognition are fundamental biological processes, warranting detailed studies into the molecular composition of gametes. Biological MS has been instrumental for the comprehensive itemizing of gamete proteomes. The protein constellation of sperm cells and its subcellular structures has been established for a variety of animal species. Spermatogenesis and the crucial activation of sperm cells as a prerequisite of successful fertilization and physiological adaptations to external stressors was investigated using proteomics, as well as the underlying mechanisms of male infertility with respect to proteome‐wide alterations. This review outlines recent achievements of sperm proteomics and exemplifies the usefulness of gel‐based surveys by outlining the comparative analysis of abnormal spermatozoa in globozoospermia. Besides label‐free MS techniques and cell‐based labeling methodology, high‐resolution fluorescence 2DE has been shown to be highly suitable as a proteomic biomarker discovery tool in sperm protein research. The appropriateness of novel protein markers for improving our understanding of normal spermatogenesis and sperm activation versus the molecular pathogenesis of male infertility will be discussed. New biomarker candidates might be useful to improve diagnostic, prognostic, and therapeutic aspects of infertility. |
| doi_str_mv | 10.1002/pmic.201400032 |
| format | Article |
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Gametogenesis, sperm activation, and egg–sperm recognition are fundamental biological processes, warranting detailed studies into the molecular composition of gametes. Biological MS has been instrumental for the comprehensive itemizing of gamete proteomes. The protein constellation of sperm cells and its subcellular structures has been established for a variety of animal species. Spermatogenesis and the crucial activation of sperm cells as a prerequisite of successful fertilization and physiological adaptations to external stressors was investigated using proteomics, as well as the underlying mechanisms of male infertility with respect to proteome‐wide alterations. This review outlines recent achievements of sperm proteomics and exemplifies the usefulness of gel‐based surveys by outlining the comparative analysis of abnormal spermatozoa in globozoospermia. Besides label‐free MS techniques and cell‐based labeling methodology, high‐resolution fluorescence 2DE has been shown to be highly suitable as a proteomic biomarker discovery tool in sperm protein research. The appropriateness of novel protein markers for improving our understanding of normal spermatogenesis and sperm activation versus the molecular pathogenesis of male infertility will be discussed. 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Gametogenesis, sperm activation, and egg–sperm recognition are fundamental biological processes, warranting detailed studies into the molecular composition of gametes. Biological MS has been instrumental for the comprehensive itemizing of gamete proteomes. The protein constellation of sperm cells and its subcellular structures has been established for a variety of animal species. Spermatogenesis and the crucial activation of sperm cells as a prerequisite of successful fertilization and physiological adaptations to external stressors was investigated using proteomics, as well as the underlying mechanisms of male infertility with respect to proteome‐wide alterations. This review outlines recent achievements of sperm proteomics and exemplifies the usefulness of gel‐based surveys by outlining the comparative analysis of abnormal spermatozoa in globozoospermia. Besides label‐free MS techniques and cell‐based labeling methodology, high‐resolution fluorescence 2DE has been shown to be highly suitable as a proteomic biomarker discovery tool in sperm protein research. The appropriateness of novel protein markers for improving our understanding of normal spermatogenesis and sperm activation versus the molecular pathogenesis of male infertility will be discussed. New biomarker candidates might be useful to improve diagnostic, prognostic, and therapeutic aspects of infertility.</description><subject>Animal proteomics</subject><subject>Animal species</subject><subject>Animals</subject><subject>Fertilization</subject><subject>Globozoospermia</subject><subject>Humans</subject><subject>Infertility</subject><subject>Male</subject><subject>Proteins</subject><subject>Proteome - analysis</subject><subject>Proteome - chemistry</subject><subject>Proteome - metabolism</subject><subject>Proteomics</subject><subject>Proteomics - methods</subject><subject>Sperm</subject><subject>Spermatogenesis</subject><subject>Spermatozoa</subject><subject>Spermatozoa - chemistry</subject><subject>Spermatozoa - metabolism</subject><issn>1615-9853</issn><issn>1615-9861</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtLw0AQxhdRrFavHiXgRZDUfT-OGmyt1AeoCF6WTbLR1KSJu6na_96E1h686GmG4fd9M_MBcIDgAEGIT-syTwYYIgohJHgD7CCOWKgkR5vrnpEe2PV-CiESUolt0MNUQYUI3gEnUVXWxpkm_7BB7aosL_LZS1BlQfNqA19bV3bjxlal3QNbmSm83V_VPngcXjxEl-HkdjSOziZhQomiYYpkyiRKTbuNpYpIkTIuheDSUmyoSYjCAqWcIJipjMo4TpXhEltC4kwmivTB8dK3Xfw-t77RZe4TWxRmZqu514hzihSUhP0DZYxhgiVt0aNf6LSau1n7SEdRITFWsKUGSypxlffOZrp2eWncQiOou8R1l7heJ94KDle287i06Rr_ibgF6BL4zAu7-MNO312PIyFEd264lOW-sV9rmXFvmgsimH66GWl5dS8n589Dzcg3wc-YEA</recordid><startdate>201502</startdate><enddate>201502</enddate><creator>Holland, Ashling</creator><creator>Ohlendieck, Kay</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201502</creationdate><title>Comparative profiling of the sperm proteome</title><author>Holland, Ashling ; Ohlendieck, Kay</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4394-d18d581da1785d9387d5687768e42a4ac39271d6310f9f48bbd9a682e33bf8c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animal proteomics</topic><topic>Animal species</topic><topic>Animals</topic><topic>Fertilization</topic><topic>Globozoospermia</topic><topic>Humans</topic><topic>Infertility</topic><topic>Male</topic><topic>Proteins</topic><topic>Proteome - analysis</topic><topic>Proteome - chemistry</topic><topic>Proteome - metabolism</topic><topic>Proteomics</topic><topic>Proteomics - methods</topic><topic>Sperm</topic><topic>Spermatogenesis</topic><topic>Spermatozoa</topic><topic>Spermatozoa - chemistry</topic><topic>Spermatozoa - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Holland, Ashling</creatorcontrib><creatorcontrib>Ohlendieck, Kay</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Proteomics (Weinheim)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Holland, Ashling</au><au>Ohlendieck, Kay</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative profiling of the sperm proteome</atitle><jtitle>Proteomics (Weinheim)</jtitle><addtitle>Proteomics</addtitle><date>2015-02</date><risdate>2015</risdate><volume>15</volume><issue>4</issue><spage>632</spage><epage>648</epage><pages>632-648</pages><issn>1615-9853</issn><eissn>1615-9861</eissn><abstract>The highly complex and species‐selective mechanism of fertilization is a central theme of developmental biology. Gametogenesis, sperm activation, and egg–sperm recognition are fundamental biological processes, warranting detailed studies into the molecular composition of gametes. Biological MS has been instrumental for the comprehensive itemizing of gamete proteomes. The protein constellation of sperm cells and its subcellular structures has been established for a variety of animal species. Spermatogenesis and the crucial activation of sperm cells as a prerequisite of successful fertilization and physiological adaptations to external stressors was investigated using proteomics, as well as the underlying mechanisms of male infertility with respect to proteome‐wide alterations. This review outlines recent achievements of sperm proteomics and exemplifies the usefulness of gel‐based surveys by outlining the comparative analysis of abnormal spermatozoa in globozoospermia. 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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Animal proteomics Animal species Animals Fertilization Globozoospermia Humans Infertility Male Proteins Proteome - analysis Proteome - chemistry Proteome - metabolism Proteomics Proteomics - methods Sperm Spermatogenesis Spermatozoa Spermatozoa - chemistry Spermatozoa - metabolism |
| title | Comparative profiling of the sperm proteome |
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