Transient expression of foreign genes introduced into barley endosperm protoplasts by PEG-mediated transfer or into intact endosperm tissue by microprojectile bombardment

Starchy endosperm protoplasts from developing barley ( Hordeum vulgare) grains were isolated 8–15 days post-anthesis. These endosperm protoplasts were incubated in a hormone free medium containing sucrose as a carbon source and glutamine as a nitrogen source. In this medium the endosperm protoplasts...

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Veröffentlicht in:	Plant science (Limerick) 1991, Vol.78 (2), p.237-246
Hauptverfasser:	Lee, Bruce T., Murdoch, Kate, Topping, Jennifer, Jones, Michael G.K., Kreis, Martin
Format:	Artikel
Sprache:	eng
Schlagworte:	barley endosperm Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology gene expression Genetic engineering Genetic technics Hordeum vulgare Methods. Procedures. Technologies Modification of gene expression level particle bombardment protoplasts
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