Quantification of alachlor in water by a novel magnetic particle-based ELISA
An enzyme-linked immunosorbent assay (ELISA) was developed for determination of the weedkiller, alachlor, in water, using a magnetic-particle solid phase. The water sample and alachlor labelled with horseradish peroxidase (HRP) were incubated for 30 minutes with the antibody-coupled solid phase, and...
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| Veröffentlicht in: | Bulletin of environmental contamination and toxicology 1992-05, Vol.48 (5), p.643-650 |
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| container_title | Bulletin of environmental contamination and toxicology |
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| creator | Lawruk, T.S. (Ohmicron Corporation, Newtown, PA) Hottenstein, C.S Herzog, D.P Rubio, F.M |
| description | An enzyme-linked immunosorbent assay (ELISA) was developed for determination of the weedkiller, alachlor, in water, using a magnetic-particle solid phase. The water sample and alachlor labelled with horseradish peroxidase (HRP) were incubated for 30 minutes with the antibody-coupled solid phase, and a magnetic field was then applied to the solid phase to remove unbound HRP-alachlor and any interfering substances. The enzyme substrate (hydrogen peroxide) and a chromogen (3,3',5,5'-tetramethylbenzidine) were then added and incubated for 20 minutes. Acid was added to stop the reaction, and the coloured product was measured with a specially-designed, microprocessor-controlled photometer. Results from validation of the method on samples of groundwater are presented. |
| doi_str_mv | 10.1007/BF00195982 |
| format | Article |
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(Ohmicron Corporation, Newtown, PA) ; Hottenstein, C.S ; Herzog, D.P ; Rubio, F.M</creator><creatorcontrib>Lawruk, T.S. (Ohmicron Corporation, Newtown, PA) ; Hottenstein, C.S ; Herzog, D.P ; Rubio, F.M</creatorcontrib><description>An enzyme-linked immunosorbent assay (ELISA) was developed for determination of the weedkiller, alachlor, in water, using a magnetic-particle solid phase. The water sample and alachlor labelled with horseradish peroxidase (HRP) were incubated for 30 minutes with the antibody-coupled solid phase, and a magnetic field was then applied to the solid phase to remove unbound HRP-alachlor and any interfering substances. The enzyme substrate (hydrogen peroxide) and a chromogen (3,3',5,5'-tetramethylbenzidine) were then added and incubated for 20 minutes. Acid was added to stop the reaction, and the coloured product was measured with a specially-designed, microprocessor-controlled photometer. Results from validation of the method on samples of groundwater are presented.</description><identifier>ISSN: 0007-4861</identifier><identifier>EISSN: 1432-0800</identifier><identifier>DOI: 10.1007/BF00195982</identifier><identifier>PMID: 1504510</identifier><identifier>CODEN: BECTA6</identifier><language>eng</language><publisher>New York, NY: Springer-Verlag</publisher><subject>Acetamides - analysis ; AGUAS SUBTERRANEAS ; ALACHLOR ; ALACHLORE ; ALACLORO ; ANALISIS CUANTITATIVO ; ANALYSE QUANTITATIVE ; Benzidines ; Biological and medical sciences ; Calibration ; CALIDAD DEL AGUA ; Chromogenic Compounds ; CLASIFICACION DE PRODUCTOS ; CLASSEMENT ; Cross Reactions ; EAU SOUTERRAINE ; ELISA ; ENSAYO ; Environmental pollutants toxicology ; Enzyme-Linked Immunosorbent Assay - methods ; Evaluation Studies as Topic ; Freshwater ; GRADING ; GROUNDWATER ; HERBICIDAS ; HERBICIDE ; HERBICIDES ; Herbicides - analysis ; Horseradish Peroxidase ; Hydrogen Peroxide ; MAGNETIC SEPARATION ; Magnetics ; Medical sciences ; QUALITE DE L'EAU ; QUANTITATIVE ANALYSIS ; RESIDU ; RESIDUES ; RESIDUOS ; TEST ELISA ; TESTAGE ; TESTING ; Toxicology ; Water ; Water Pollutants, Chemical - analysis ; WATER QUALITY</subject><ispartof>Bulletin of environmental contamination and toxicology, 1992-05, Vol.48 (5), p.643-650</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-19c590cbf24c2dd4871728ca98ea519ee8403b30d5c8b3ab3d213a0df17adf843</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5276068$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1504510$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lawruk, T.S. (Ohmicron Corporation, Newtown, PA)</creatorcontrib><creatorcontrib>Hottenstein, C.S</creatorcontrib><creatorcontrib>Herzog, D.P</creatorcontrib><creatorcontrib>Rubio, F.M</creatorcontrib><title>Quantification of alachlor in water by a novel magnetic particle-based ELISA</title><title>Bulletin of environmental contamination and toxicology</title><addtitle>Bull Environ Contam Toxicol</addtitle><description>An enzyme-linked immunosorbent assay (ELISA) was developed for determination of the weedkiller, alachlor, in water, using a magnetic-particle solid phase. The water sample and alachlor labelled with horseradish peroxidase (HRP) were incubated for 30 minutes with the antibody-coupled solid phase, and a magnetic field was then applied to the solid phase to remove unbound HRP-alachlor and any interfering substances. The enzyme substrate (hydrogen peroxide) and a chromogen (3,3',5,5'-tetramethylbenzidine) were then added and incubated for 20 minutes. Acid was added to stop the reaction, and the coloured product was measured with a specially-designed, microprocessor-controlled photometer. Results from validation of the method on samples of groundwater are presented.</description><subject>Acetamides - analysis</subject><subject>AGUAS SUBTERRANEAS</subject><subject>ALACHLOR</subject><subject>ALACHLORE</subject><subject>ALACLORO</subject><subject>ANALISIS CUANTITATIVO</subject><subject>ANALYSE QUANTITATIVE</subject><subject>Benzidines</subject><subject>Biological and medical sciences</subject><subject>Calibration</subject><subject>CALIDAD DEL AGUA</subject><subject>Chromogenic Compounds</subject><subject>CLASIFICACION DE PRODUCTOS</subject><subject>CLASSEMENT</subject><subject>Cross Reactions</subject><subject>EAU SOUTERRAINE</subject><subject>ELISA</subject><subject>ENSAYO</subject><subject>Environmental pollutants toxicology</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Evaluation Studies as Topic</subject><subject>Freshwater</subject><subject>GRADING</subject><subject>GROUNDWATER</subject><subject>HERBICIDAS</subject><subject>HERBICIDE</subject><subject>HERBICIDES</subject><subject>Herbicides - analysis</subject><subject>Horseradish Peroxidase</subject><subject>Hydrogen Peroxide</subject><subject>MAGNETIC SEPARATION</subject><subject>Magnetics</subject><subject>Medical sciences</subject><subject>QUALITE DE L'EAU</subject><subject>QUANTITATIVE ANALYSIS</subject><subject>RESIDU</subject><subject>RESIDUES</subject><subject>RESIDUOS</subject><subject>TEST ELISA</subject><subject>TESTAGE</subject><subject>TESTING</subject><subject>Toxicology</subject><subject>Water</subject><subject>Water Pollutants, Chemical - analysis</subject><subject>WATER QUALITY</subject><issn>0007-4861</issn><issn>1432-0800</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0EtLxDAUBeAgio6PjUtByEJcCNV7k6ZNliq-YEBEXZfbNNFKpx2TjuK_NzKDLl2dxfk4i8PYPsIpApRnF9cAaJTRYo1NMJciAw2wziaQ2izXBW6x7RjfklJaiE22iQpyhTBh04cF9WPrW0tjO_R88Jw6sq_dEHjb808aXeD1FyfeDx-u4zN66d3YWj6nkKJzWU3RNfxqevd4vss2PHXR7a1yhz1fXz1d3mbT-5u7y_NpZqURY4bGKgO29iK3omlyXWIptCWjHSk0zukcZC2hUVbXkmrZCJQEjceSGq9zucOOl7vzMLwvXByrWRut6zrq3bCIFRaFSHfg_1CWqJQxCZ4soQ1DjMH5ah7aGYWvCqH6-bj6-zjhw9Xqop655o8uT0390aqnaKnzgXrbxl-mRFlAoRM7WDJPQ0UvIZHnR4NaFbKU3-LbibA</recordid><startdate>19920501</startdate><enddate>19920501</enddate><creator>Lawruk, T.S. (Ohmicron Corporation, Newtown, PA)</creator><creator>Hottenstein, C.S</creator><creator>Herzog, D.P</creator><creator>Rubio, F.M</creator><general>Springer-Verlag</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7TV</scope><scope>7UA</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H97</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>19920501</creationdate><title>Quantification of alachlor in water by a novel magnetic particle-based ELISA</title><author>Lawruk, T.S. (Ohmicron Corporation, Newtown, PA) ; Hottenstein, C.S ; Herzog, D.P ; Rubio, F.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-19c590cbf24c2dd4871728ca98ea519ee8403b30d5c8b3ab3d213a0df17adf843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Acetamides - analysis</topic><topic>AGUAS SUBTERRANEAS</topic><topic>ALACHLOR</topic><topic>ALACHLORE</topic><topic>ALACLORO</topic><topic>ANALISIS CUANTITATIVO</topic><topic>ANALYSE QUANTITATIVE</topic><topic>Benzidines</topic><topic>Biological and medical sciences</topic><topic>Calibration</topic><topic>CALIDAD DEL AGUA</topic><topic>Chromogenic Compounds</topic><topic>CLASIFICACION DE PRODUCTOS</topic><topic>CLASSEMENT</topic><topic>Cross Reactions</topic><topic>EAU SOUTERRAINE</topic><topic>ELISA</topic><topic>ENSAYO</topic><topic>Environmental pollutants toxicology</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Evaluation Studies as Topic</topic><topic>Freshwater</topic><topic>GRADING</topic><topic>GROUNDWATER</topic><topic>HERBICIDAS</topic><topic>HERBICIDE</topic><topic>HERBICIDES</topic><topic>Herbicides - analysis</topic><topic>Horseradish Peroxidase</topic><topic>Hydrogen Peroxide</topic><topic>MAGNETIC SEPARATION</topic><topic>Magnetics</topic><topic>Medical sciences</topic><topic>QUALITE DE L'EAU</topic><topic>QUANTITATIVE ANALYSIS</topic><topic>RESIDU</topic><topic>RESIDUES</topic><topic>RESIDUOS</topic><topic>TEST ELISA</topic><topic>TESTAGE</topic><topic>TESTING</topic><topic>Toxicology</topic><topic>Water</topic><topic>Water Pollutants, Chemical - analysis</topic><topic>WATER QUALITY</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lawruk, T.S. (Ohmicron Corporation, Newtown, PA)</creatorcontrib><creatorcontrib>Hottenstein, C.S</creatorcontrib><creatorcontrib>Herzog, D.P</creatorcontrib><creatorcontrib>Rubio, F.M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Pollution Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Bulletin of environmental contamination and toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lawruk, T.S. (Ohmicron Corporation, Newtown, PA)</au><au>Hottenstein, C.S</au><au>Herzog, D.P</au><au>Rubio, F.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of alachlor in water by a novel magnetic particle-based ELISA</atitle><jtitle>Bulletin of environmental contamination and toxicology</jtitle><addtitle>Bull Environ Contam Toxicol</addtitle><date>1992-05-01</date><risdate>1992</risdate><volume>48</volume><issue>5</issue><spage>643</spage><epage>650</epage><pages>643-650</pages><issn>0007-4861</issn><eissn>1432-0800</eissn><coden>BECTA6</coden><abstract>An enzyme-linked immunosorbent assay (ELISA) was developed for determination of the weedkiller, alachlor, in water, using a magnetic-particle solid phase. The water sample and alachlor labelled with horseradish peroxidase (HRP) were incubated for 30 minutes with the antibody-coupled solid phase, and a magnetic field was then applied to the solid phase to remove unbound HRP-alachlor and any interfering substances. The enzyme substrate (hydrogen peroxide) and a chromogen (3,3',5,5'-tetramethylbenzidine) were then added and incubated for 20 minutes. Acid was added to stop the reaction, and the coloured product was measured with a specially-designed, microprocessor-controlled photometer. Results from validation of the method on samples of groundwater are presented.</abstract><cop>New York, NY</cop><pub>Springer-Verlag</pub><pmid>1504510</pmid><doi>10.1007/BF00195982</doi><tpages>8</tpages></addata></record> |
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| ispartof | Bulletin of environmental contamination and toxicology, 1992-05, Vol.48 (5), p.643-650 |
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| subjects | Acetamides - analysis AGUAS SUBTERRANEAS ALACHLOR ALACHLORE ALACLORO ANALISIS CUANTITATIVO ANALYSE QUANTITATIVE Benzidines Biological and medical sciences Calibration CALIDAD DEL AGUA Chromogenic Compounds CLASIFICACION DE PRODUCTOS CLASSEMENT Cross Reactions EAU SOUTERRAINE ELISA ENSAYO Environmental pollutants toxicology Enzyme-Linked Immunosorbent Assay - methods Evaluation Studies as Topic Freshwater GRADING GROUNDWATER HERBICIDAS HERBICIDE HERBICIDES Herbicides - analysis Horseradish Peroxidase Hydrogen Peroxide MAGNETIC SEPARATION Magnetics Medical sciences QUALITE DE L'EAU QUANTITATIVE ANALYSIS RESIDU RESIDUES RESIDUOS TEST ELISA TESTAGE TESTING Toxicology Water Water Pollutants, Chemical - analysis WATER QUALITY |
| title | Quantification of alachlor in water by a novel magnetic particle-based ELISA |
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