Thermophilic anaerobic digestion of thermal pretreated sludge: Role of microbial community structure and correlation with process performances
Thermal hydrolysis pretreatment coupled with Thermophilic Anaerobic Digestion (TAD) for Waste Activated Sludge (WAS) treatment is a promising combination to improve biodegradation kinetics during stabilization. However, to date there is a limited knowledge of the anaerobic biomass composition and it...
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| Veröffentlicht in: | Water research (Oxford) 2015-01, Vol.68, p.498-509 |
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| creator | Gagliano, M.C. Braguglia, C.M. Gianico, A. Mininni, G. Nakamura, K. Rossetti, S. |
| description | Thermal hydrolysis pretreatment coupled with Thermophilic Anaerobic Digestion (TAD) for Waste Activated Sludge (WAS) treatment is a promising combination to improve biodegradation kinetics during stabilization. However, to date there is a limited knowledge of the anaerobic biomass composition and its impact on TAD process performances.
In this study, the structure and dynamics of the microbial communities selected in two semi-continuous anaerobic digesters, fed with untreated and thermal pretreated sludge, were investigated. The systems were operated for 250 days at different organic loading rate.
16S rRNA gene clonal analysis and Fluorescence In Situ Hybridization (FISH) analyses allowed us to identify the majority of bacterial and archaeal populations. Proteolytic Coprothermobacter spp. and hydrogenotrophic Methanothermobacter spp. living in strict syntrophic association were found to dominate in TAD process.
The establishment of a syntrophic proteolytic pathway was favoured by the high temperature of the process and enhanced by the thermal pretreatment of the feeding sludge. Proteolytic activity, alone or with thermal pretreatment, occurred during TAD as proven by increasing concentration of soluble ammonia and soluble COD (sCOD) during the process. However, the availability of a readily biodegradable substrate due to pretreatment allowed to significant sCOD removals (more than 55%) corresponding to higher biogas production in the reactor fed with thermal pretreated sludge. Microbial population dynamics analysed by FISH showed that Coprothermobacter and Methanothermobacter immediately established a stable syntrophic association in the reactor fed with pretreated sludge in line with the overall improved TAD performances observed under these conditions.
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•Thermal pretreatment improved anaerobic solids reduction and methane yields.•Establishment of proteolytic fermentative and hydrogenotrophic methanogenic pathways.•First evidence of Coprothermobacter dominance in a thermophilic AD. |
| doi_str_mv | 10.1016/j.watres.2014.10.031 |
| format | Article |
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In this study, the structure and dynamics of the microbial communities selected in two semi-continuous anaerobic digesters, fed with untreated and thermal pretreated sludge, were investigated. The systems were operated for 250 days at different organic loading rate.
16S rRNA gene clonal analysis and Fluorescence In Situ Hybridization (FISH) analyses allowed us to identify the majority of bacterial and archaeal populations. Proteolytic Coprothermobacter spp. and hydrogenotrophic Methanothermobacter spp. living in strict syntrophic association were found to dominate in TAD process.
The establishment of a syntrophic proteolytic pathway was favoured by the high temperature of the process and enhanced by the thermal pretreatment of the feeding sludge. Proteolytic activity, alone or with thermal pretreatment, occurred during TAD as proven by increasing concentration of soluble ammonia and soluble COD (sCOD) during the process. However, the availability of a readily biodegradable substrate due to pretreatment allowed to significant sCOD removals (more than 55%) corresponding to higher biogas production in the reactor fed with thermal pretreated sludge. Microbial population dynamics analysed by FISH showed that Coprothermobacter and Methanothermobacter immediately established a stable syntrophic association in the reactor fed with pretreated sludge in line with the overall improved TAD performances observed under these conditions.
[Display omitted]
•Thermal pretreatment improved anaerobic solids reduction and methane yields.•Establishment of proteolytic fermentative and hydrogenotrophic methanogenic pathways.•First evidence of Coprothermobacter dominance in a thermophilic AD.</description><identifier>ISSN: 0043-1354</identifier><identifier>EISSN: 1879-2448</identifier><identifier>DOI: 10.1016/j.watres.2014.10.031</identifier><identifier>PMID: 25462756</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Anaerobic digestion ; Anaerobiosis ; Archaea - classification ; Archaea - genetics ; Archaea - metabolism ; Bacteria ; Bacteria - classification ; Bacteria - genetics ; Bacteria - metabolism ; Biodegradation, Environmental ; Biomass ; Bioreactors - microbiology ; Coprothermobacter ; Dynamics ; Fluorescence in situ hybridization ; Hot Temperature ; In Situ Hybridization, Fluorescence ; Methane - metabolism ; Methanobacteriaceae - genetics ; Methanobacteriaceae - metabolism ; Methanothermobacter ; Microorganisms ; Molecular Sequence Data ; Polymerase Chain Reaction ; Populations ; Pretreatment ; Reactors ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Sewage - microbiology ; Sludge ; Thermal hydrolysis ; Thermophilic anaerobic digestion ; Water Microbiology</subject><ispartof>Water research (Oxford), 2015-01, Vol.68, p.498-509</ispartof><rights>2014 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-d2078ecb44006ad9ac956059710d84a3a30ceda73ebf02218a5d86b67af8f4333</citedby><cites>FETCH-LOGICAL-c428t-d2078ecb44006ad9ac956059710d84a3a30ceda73ebf02218a5d86b67af8f4333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0043135414007295$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25462756$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gagliano, M.C.</creatorcontrib><creatorcontrib>Braguglia, C.M.</creatorcontrib><creatorcontrib>Gianico, A.</creatorcontrib><creatorcontrib>Mininni, G.</creatorcontrib><creatorcontrib>Nakamura, K.</creatorcontrib><creatorcontrib>Rossetti, S.</creatorcontrib><title>Thermophilic anaerobic digestion of thermal pretreated sludge: Role of microbial community structure and correlation with process performances</title><title>Water research (Oxford)</title><addtitle>Water Res</addtitle><description>Thermal hydrolysis pretreatment coupled with Thermophilic Anaerobic Digestion (TAD) for Waste Activated Sludge (WAS) treatment is a promising combination to improve biodegradation kinetics during stabilization. However, to date there is a limited knowledge of the anaerobic biomass composition and its impact on TAD process performances.
In this study, the structure and dynamics of the microbial communities selected in two semi-continuous anaerobic digesters, fed with untreated and thermal pretreated sludge, were investigated. The systems were operated for 250 days at different organic loading rate.
16S rRNA gene clonal analysis and Fluorescence In Situ Hybridization (FISH) analyses allowed us to identify the majority of bacterial and archaeal populations. Proteolytic Coprothermobacter spp. and hydrogenotrophic Methanothermobacter spp. living in strict syntrophic association were found to dominate in TAD process.
The establishment of a syntrophic proteolytic pathway was favoured by the high temperature of the process and enhanced by the thermal pretreatment of the feeding sludge. Proteolytic activity, alone or with thermal pretreatment, occurred during TAD as proven by increasing concentration of soluble ammonia and soluble COD (sCOD) during the process. However, the availability of a readily biodegradable substrate due to pretreatment allowed to significant sCOD removals (more than 55%) corresponding to higher biogas production in the reactor fed with thermal pretreated sludge. Microbial population dynamics analysed by FISH showed that Coprothermobacter and Methanothermobacter immediately established a stable syntrophic association in the reactor fed with pretreated sludge in line with the overall improved TAD performances observed under these conditions.
[Display omitted]
•Thermal pretreatment improved anaerobic solids reduction and methane yields.•Establishment of proteolytic fermentative and hydrogenotrophic methanogenic pathways.•First evidence of Coprothermobacter dominance in a thermophilic AD.</description><subject>Anaerobic digestion</subject><subject>Anaerobiosis</subject><subject>Archaea - classification</subject><subject>Archaea - genetics</subject><subject>Archaea - metabolism</subject><subject>Bacteria</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - metabolism</subject><subject>Biodegradation, Environmental</subject><subject>Biomass</subject><subject>Bioreactors - microbiology</subject><subject>Coprothermobacter</subject><subject>Dynamics</subject><subject>Fluorescence in situ hybridization</subject><subject>Hot Temperature</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Methane - metabolism</subject><subject>Methanobacteriaceae - genetics</subject><subject>Methanobacteriaceae - metabolism</subject><subject>Methanothermobacter</subject><subject>Microorganisms</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction</subject><subject>Populations</subject><subject>Pretreatment</subject><subject>Reactors</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Sewage - microbiology</subject><subject>Sludge</subject><subject>Thermal hydrolysis</subject><subject>Thermophilic anaerobic digestion</subject><subject>Water Microbiology</subject><issn>0043-1354</issn><issn>1879-2448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9u1DAQxi1ERbeFN0AoRy5Zxn_iOByQUAUUqVKlqj1bjj3pepXEi-1Q9SV4Zhy2cEQ92R795vs88xHylsKWApUf9tsHkyOmLQMqSmkLnL4gG6rarmZCqJdkAyB4TXkjTslZSnsAYIx3r8gpa4RkbSM35NftDuMUDjs_eluZ2WAMfbk5f48p-zBXYajyypixOkQsjiajq9K4uHv8WN2EEVdk8nZtLJAN07TMPj9WKcfF5iVi0XWlHiOO5o_mg8-7ohYsplQdMA6h6M_l9ZqcDGZM-ObpPCd3X7_cXlzWV9ffvl98vqqtYCrXjkGr0PZCAEjjOmO7RkLTtRScEoYbDhadaTn2Q5mZKtM4JXvZmkENgnN-Tt4fdcsnfixlUj35ZHEczYxhSZpKyQTrQDXPQQFa1lDxDFS0QBWHFRVHtKwtpYiDPkQ_mfioKeg1X73Xx3z1mu9aLfmWtndPDks_ofvX9DfQAnw6Ali299Nj1Ml6LKt1PqLN2gX_f4ffTNe7ig</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Gagliano, M.C.</creator><creator>Braguglia, C.M.</creator><creator>Gianico, A.</creator><creator>Mininni, G.</creator><creator>Nakamura, K.</creator><creator>Rossetti, S.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7ST</scope><scope>7T7</scope><scope>7UA</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H97</scope><scope>L.G</scope><scope>P64</scope><scope>SOI</scope><scope>KR7</scope><scope>7X8</scope></search><sort><creationdate>20150101</creationdate><title>Thermophilic anaerobic digestion of thermal pretreated sludge: Role of microbial community structure and correlation with process performances</title><author>Gagliano, M.C. ; 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However, to date there is a limited knowledge of the anaerobic biomass composition and its impact on TAD process performances.
In this study, the structure and dynamics of the microbial communities selected in two semi-continuous anaerobic digesters, fed with untreated and thermal pretreated sludge, were investigated. The systems were operated for 250 days at different organic loading rate.
16S rRNA gene clonal analysis and Fluorescence In Situ Hybridization (FISH) analyses allowed us to identify the majority of bacterial and archaeal populations. Proteolytic Coprothermobacter spp. and hydrogenotrophic Methanothermobacter spp. living in strict syntrophic association were found to dominate in TAD process.
The establishment of a syntrophic proteolytic pathway was favoured by the high temperature of the process and enhanced by the thermal pretreatment of the feeding sludge. Proteolytic activity, alone or with thermal pretreatment, occurred during TAD as proven by increasing concentration of soluble ammonia and soluble COD (sCOD) during the process. However, the availability of a readily biodegradable substrate due to pretreatment allowed to significant sCOD removals (more than 55%) corresponding to higher biogas production in the reactor fed with thermal pretreated sludge. Microbial population dynamics analysed by FISH showed that Coprothermobacter and Methanothermobacter immediately established a stable syntrophic association in the reactor fed with pretreated sludge in line with the overall improved TAD performances observed under these conditions.
[Display omitted]
•Thermal pretreatment improved anaerobic solids reduction and methane yields.•Establishment of proteolytic fermentative and hydrogenotrophic methanogenic pathways.•First evidence of Coprothermobacter dominance in a thermophilic AD.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>25462756</pmid><doi>10.1016/j.watres.2014.10.031</doi><tpages>12</tpages></addata></record> |
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| subjects | Anaerobic digestion Anaerobiosis Archaea - classification Archaea - genetics Archaea - metabolism Bacteria Bacteria - classification Bacteria - genetics Bacteria - metabolism Biodegradation, Environmental Biomass Bioreactors - microbiology Coprothermobacter Dynamics Fluorescence in situ hybridization Hot Temperature In Situ Hybridization, Fluorescence Methane - metabolism Methanobacteriaceae - genetics Methanobacteriaceae - metabolism Methanothermobacter Microorganisms Molecular Sequence Data Polymerase Chain Reaction Populations Pretreatment Reactors RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Sewage - microbiology Sludge Thermal hydrolysis Thermophilic anaerobic digestion Water Microbiology |
| title | Thermophilic anaerobic digestion of thermal pretreated sludge: Role of microbial community structure and correlation with process performances |
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