Site‐specific insertion of gene cassettes into integrons

Summary Site‐specific insertion of gene cassettes into the insert region of integrons has been demonstrated. Insertion was only observed if the integron DNA integrase was expressed in the recipient cell and if the cassette DNA was ligated prior to transformation. The essential ligation products were...

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Veröffentlicht in:	Molecular microbiology 1993-07, Vol.9 (1), p.41-52
Hauptverfasser:	Collis, Christina M., Grammaticopoulos, Georgia, Briton, Jayne, Stokes, H.W., Hall, Ruth M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriology Base Sequence Biological and medical sciences DNA Nucleotidyltransferases - genetics DNA Transposable Elements - genetics DNA, Bacterial - genetics DNA, Circular - genetics DNA, Recombinant - genetics Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Genetics Integrases Microbiology Molecular Sequence Data Mutagenesis, Insertional Mutagenesis, Site-Directed Recombination, Genetic
Online-Zugang:	Volltext
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