Site‐specific insertion of gene cassettes into integrons
Summary Site‐specific insertion of gene cassettes into the insert region of integrons has been demonstrated. Insertion was only observed if the integron DNA integrase was expressed in the recipient cell and if the cassette DNA was ligated prior to transformation. The essential ligation products were...
Gespeichert in:
| Veröffentlicht in: | Molecular microbiology 1993-07, Vol.9 (1), p.41-52 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 52 |
|---|---|
| container_issue | 1 |
| container_start_page | 41 |
| container_title | Molecular microbiology |
| container_volume | 9 |
| creator | Collis, Christina M. Grammaticopoulos, Georgia Briton, Jayne Stokes, H.W. Hall, Ruth M. |
| description | Summary
Site‐specific insertion of gene cassettes into the insert region of integrons has been demonstrated. Insertion was only observed if the integron DNA integrase was expressed in the recipient cell and if the cassette DNA was ligated prior to transformation. The essential ligation products were resistant to treatment with exonuclease III, indicating that they were closed circular molecules. Insertion of cassettes into integron fragments containing either no insert (one recombination site), or one gene cassette (two recombination sites), was demonstrated. In the latter case, insertion occurred predominantly at the core site located 5′ to the resident cassette, which corresponds to the only site available when no insert is present in the recipient. When DNA molecules including two gene cassettes were used, insertion of only one of the gene cassettes was generally observed, suggesting that resolution of the circular molecule to generate two independent circular cassettes occurred more rapidly than insertion into the recipient integron. |
| doi_str_mv | 10.1111/j.1365-2958.1993.tb01667.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_16623736</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16623736</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4281-ed6eadd2d890b06e5fb6720bf7a8c87fe9d39c689c9d15fb631a54f21ea9f2a43</originalsourceid><addsrcrecordid>eNqVkN1KwzAUx4Moc04fQSgi3rXmo02bgRcy_BhseKGCdyFNT0ZG186kw-3OR_AZfRJbVnZvLk4ufv9zcvJD6IrgiLTndhkRxpOQiiSLiBAsanJMOE-j7REaHtAxGmKR4JBl9OMUnXm_xJgwzNkADbKYUJ7iIRq_2gZ-v3_8GrQ1Vge28uAaW1dBbYIFVBBo5T00DfiWNXVXYOHqyp-jE6NKDxf9PULvjw9vk-dw9vI0ndzPQh3TjIRQcFBFQYtM4BxzSEzOU4pzk6pMZ6kBUTCheSa0KEgHGVFJbCgBJQxVMRuhm_3ctas_N-AbubJeQ1mqCuqNl-3PKUsZb4PjfVC72nsHRq6dXSm3kwTLTpxcys6O7OzITpzsxclt23zZv7LJV1AcWntTLb_uufJalcapSlt_iMWCcMq7Ze_2sS9bwu4fC8j5fBoT9gfTsYwO</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16623736</pqid></control><display><type>article</type><title>Site‐specific insertion of gene cassettes into integrons</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Collis, Christina M. ; Grammaticopoulos, Georgia ; Briton, Jayne ; Stokes, H.W. ; Hall, Ruth M.</creator><creatorcontrib>Collis, Christina M. ; Grammaticopoulos, Georgia ; Briton, Jayne ; Stokes, H.W. ; Hall, Ruth M.</creatorcontrib><description>Summary
Site‐specific insertion of gene cassettes into the insert region of integrons has been demonstrated. Insertion was only observed if the integron DNA integrase was expressed in the recipient cell and if the cassette DNA was ligated prior to transformation. The essential ligation products were resistant to treatment with exonuclease III, indicating that they were closed circular molecules. Insertion of cassettes into integron fragments containing either no insert (one recombination site), or one gene cassette (two recombination sites), was demonstrated. In the latter case, insertion occurred predominantly at the core site located 5′ to the resident cassette, which corresponds to the only site available when no insert is present in the recipient. When DNA molecules including two gene cassettes were used, insertion of only one of the gene cassettes was generally observed, suggesting that resolution of the circular molecule to generate two independent circular cassettes occurred more rapidly than insertion into the recipient integron.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.1993.tb01667.x</identifier><identifier>PMID: 8412670</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacteriology ; Base Sequence ; Biological and medical sciences ; DNA Nucleotidyltransferases - genetics ; DNA Transposable Elements - genetics ; DNA, Bacterial - genetics ; DNA, Circular - genetics ; DNA, Recombinant - genetics ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial ; Genetics ; Integrases ; Microbiology ; Molecular Sequence Data ; Mutagenesis, Insertional ; Mutagenesis, Site-Directed ; Recombination, Genetic</subject><ispartof>Molecular microbiology, 1993-07, Vol.9 (1), p.41-52</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4281-ed6eadd2d890b06e5fb6720bf7a8c87fe9d39c689c9d15fb631a54f21ea9f2a43</citedby><cites>FETCH-LOGICAL-c4281-ed6eadd2d890b06e5fb6720bf7a8c87fe9d39c689c9d15fb631a54f21ea9f2a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2958.1993.tb01667.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2958.1993.tb01667.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4916264$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8412670$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Collis, Christina M.</creatorcontrib><creatorcontrib>Grammaticopoulos, Georgia</creatorcontrib><creatorcontrib>Briton, Jayne</creatorcontrib><creatorcontrib>Stokes, H.W.</creatorcontrib><creatorcontrib>Hall, Ruth M.</creatorcontrib><title>Site‐specific insertion of gene cassettes into integrons</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
Site‐specific insertion of gene cassettes into the insert region of integrons has been demonstrated. Insertion was only observed if the integron DNA integrase was expressed in the recipient cell and if the cassette DNA was ligated prior to transformation. The essential ligation products were resistant to treatment with exonuclease III, indicating that they were closed circular molecules. Insertion of cassettes into integron fragments containing either no insert (one recombination site), or one gene cassette (two recombination sites), was demonstrated. In the latter case, insertion occurred predominantly at the core site located 5′ to the resident cassette, which corresponds to the only site available when no insert is present in the recipient. When DNA molecules including two gene cassettes were used, insertion of only one of the gene cassettes was generally observed, suggesting that resolution of the circular molecule to generate two independent circular cassettes occurred more rapidly than insertion into the recipient integron.</description><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA Nucleotidyltransferases - genetics</subject><subject>DNA Transposable Elements - genetics</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Circular - genetics</subject><subject>DNA, Recombinant - genetics</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genetics</subject><subject>Integrases</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis, Insertional</subject><subject>Mutagenesis, Site-Directed</subject><subject>Recombination, Genetic</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkN1KwzAUx4Moc04fQSgi3rXmo02bgRcy_BhseKGCdyFNT0ZG186kw-3OR_AZfRJbVnZvLk4ufv9zcvJD6IrgiLTndhkRxpOQiiSLiBAsanJMOE-j7REaHtAxGmKR4JBl9OMUnXm_xJgwzNkADbKYUJ7iIRq_2gZ-v3_8GrQ1Vge28uAaW1dBbYIFVBBo5T00DfiWNXVXYOHqyp-jE6NKDxf9PULvjw9vk-dw9vI0ndzPQh3TjIRQcFBFQYtM4BxzSEzOU4pzk6pMZ6kBUTCheSa0KEgHGVFJbCgBJQxVMRuhm_3ctas_N-AbubJeQ1mqCuqNl-3PKUsZb4PjfVC72nsHRq6dXSm3kwTLTpxcys6O7OzITpzsxclt23zZv7LJV1AcWntTLb_uufJalcapSlt_iMWCcMq7Ze_2sS9bwu4fC8j5fBoT9gfTsYwO</recordid><startdate>199307</startdate><enddate>199307</enddate><creator>Collis, Christina M.</creator><creator>Grammaticopoulos, Georgia</creator><creator>Briton, Jayne</creator><creator>Stokes, H.W.</creator><creator>Hall, Ruth M.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>199307</creationdate><title>Site‐specific insertion of gene cassettes into integrons</title><author>Collis, Christina M. ; Grammaticopoulos, Georgia ; Briton, Jayne ; Stokes, H.W. ; Hall, Ruth M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4281-ed6eadd2d890b06e5fb6720bf7a8c87fe9d39c689c9d15fb631a54f21ea9f2a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>DNA Nucleotidyltransferases - genetics</topic><topic>DNA Transposable Elements - genetics</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Circular - genetics</topic><topic>DNA, Recombinant - genetics</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genetics</topic><topic>Integrases</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis, Insertional</topic><topic>Mutagenesis, Site-Directed</topic><topic>Recombination, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Collis, Christina M.</creatorcontrib><creatorcontrib>Grammaticopoulos, Georgia</creatorcontrib><creatorcontrib>Briton, Jayne</creatorcontrib><creatorcontrib>Stokes, H.W.</creatorcontrib><creatorcontrib>Hall, Ruth M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Collis, Christina M.</au><au>Grammaticopoulos, Georgia</au><au>Briton, Jayne</au><au>Stokes, H.W.</au><au>Hall, Ruth M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Site‐specific insertion of gene cassettes into integrons</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>1993-07</date><risdate>1993</risdate><volume>9</volume><issue>1</issue><spage>41</spage><epage>52</epage><pages>41-52</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
Site‐specific insertion of gene cassettes into the insert region of integrons has been demonstrated. Insertion was only observed if the integron DNA integrase was expressed in the recipient cell and if the cassette DNA was ligated prior to transformation. The essential ligation products were resistant to treatment with exonuclease III, indicating that they were closed circular molecules. Insertion of cassettes into integron fragments containing either no insert (one recombination site), or one gene cassette (two recombination sites), was demonstrated. In the latter case, insertion occurred predominantly at the core site located 5′ to the resident cassette, which corresponds to the only site available when no insert is present in the recipient. When DNA molecules including two gene cassettes were used, insertion of only one of the gene cassettes was generally observed, suggesting that resolution of the circular molecule to generate two independent circular cassettes occurred more rapidly than insertion into the recipient integron.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8412670</pmid><doi>10.1111/j.1365-2958.1993.tb01667.x</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0950-382X |
| ispartof | Molecular microbiology, 1993-07, Vol.9 (1), p.41-52 |
| issn | 0950-382X 1365-2958 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16623736 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Bacteriology Base Sequence Biological and medical sciences DNA Nucleotidyltransferases - genetics DNA Transposable Elements - genetics DNA, Bacterial - genetics DNA, Circular - genetics DNA, Recombinant - genetics Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Genetics Integrases Microbiology Molecular Sequence Data Mutagenesis, Insertional Mutagenesis, Site-Directed Recombination, Genetic |
| title | Site‐specific insertion of gene cassettes into integrons |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T14%3A21%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Site%E2%80%90specific%20insertion%20of%20gene%20cassettes%20into%20integrons&rft.jtitle=Molecular%20microbiology&rft.au=Collis,%20Christina%20M.&rft.date=1993-07&rft.volume=9&rft.issue=1&rft.spage=41&rft.epage=52&rft.pages=41-52&rft.issn=0950-382X&rft.eissn=1365-2958&rft_id=info:doi/10.1111/j.1365-2958.1993.tb01667.x&rft_dat=%3Cproquest_cross%3E16623736%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16623736&rft_id=info:pmid/8412670&rfr_iscdi=true |