Construction of a biotin-overproducing strain of Serratia marcescens
We have isolated mutants resistant to acidomycin, a biotin analog, from Serratia marcescens Sr41. Strain SB304, resistant to 0.5 mg of acidomycin (frequently called actithiazic acid) per ml, produced 5 mg of D-biotin per liter of a medium containing sucrose and urea. Strain SB412, which was isolated...
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| Veröffentlicht in: | Applied and Environmental Microbiology 1993-09, Vol.59 (9), p.2857-2863 |
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| creator | Sakurai, N Imai, Y Masuda, M Komatsubara, S Tosa, T |
| description | We have isolated mutants resistant to acidomycin, a biotin analog, from Serratia marcescens Sr41. Strain SB304, resistant to 0.5 mg of acidomycin (frequently called actithiazic acid) per ml, produced 5 mg of D-biotin per liter of a medium containing sucrose and urea. Strain SB412, which was isolated from SB304 on a minimal agar plate containing 2 mg of acidomycin per ml and 0.1 mg of 5-(2-thienyl)-valeric acid per ml, produced 20 Mg of D-biotin per ml. The two enzymes related to biotin synthesis were found to be released from biotin-mediated feedback repression in these mutants. Transductional analysis revealed that SB412 had acquired at least two mutations, one in the biotin operon locus and the other in an unknown locus distant from the biotin operon locus |
| doi_str_mv | 10.1128/aem.59.9.2857-2863.1993 |
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Strain SB304, resistant to 0.5 mg of acidomycin (frequently called actithiazic acid) per ml, produced 5 mg of D-biotin per liter of a medium containing sucrose and urea. Strain SB412, which was isolated from SB304 on a minimal agar plate containing 2 mg of acidomycin per ml and 0.1 mg of 5-(2-thienyl)-valeric acid per ml, produced 20 Mg of D-biotin per ml. The two enzymes related to biotin synthesis were found to be released from biotin-mediated feedback repression in these mutants. Transductional analysis revealed that SB412 had acquired at least two mutations, one in the biotin operon locus and the other in an unknown locus distant from the biotin operon locus</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.59.9.2857-2863.1993</identifier><identifier>PMID: 16349036</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Bacteria ; Biochemistry ; Biological and medical sciences ; BIOSINTESIS ; BIOSYNTHESE ; Biotechnology ; BIOTINA ; BIOTINE ; Fundamental and applied biological sciences. Psychology ; Genetic technics ; Methods. Procedures. 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Strain SB304, resistant to 0.5 mg of acidomycin (frequently called actithiazic acid) per ml, produced 5 mg of D-biotin per liter of a medium containing sucrose and urea. Strain SB412, which was isolated from SB304 on a minimal agar plate containing 2 mg of acidomycin per ml and 0.1 mg of 5-(2-thienyl)-valeric acid per ml, produced 20 Mg of D-biotin per ml. The two enzymes related to biotin synthesis were found to be released from biotin-mediated feedback repression in these mutants. Transductional analysis revealed that SB412 had acquired at least two mutations, one in the biotin operon locus and the other in an unknown locus distant from the biotin operon locus</description><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>BIOSINTESIS</subject><subject>BIOSYNTHESE</subject><subject>Biotechnology</subject><subject>BIOTINA</subject><subject>BIOTINE</subject><subject>Fundamental and applied biological sciences. 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| source | American Society for Microbiology; PubMed Central; Alma/SFX Local Collection |
| subjects | Bacteria Biochemistry Biological and medical sciences BIOSINTESIS BIOSYNTHESE Biotechnology BIOTINA BIOTINE Fundamental and applied biological sciences. Psychology Genetic technics Methods. Procedures. Technologies MUTACION MUTANT Mutant screening MUTANTES MUTATION Physiology and Biotechnology Prokaryotes SERRATIA Serratia marcescens |
| title | Construction of a biotin-overproducing strain of Serratia marcescens |
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