Construction of His sub(6)-tagging vectors allowing single-step purification of GroES and other polypeptides produced in Bacillus subtilis

Two plasmid expression vectors for Bacillus subtilis have been constructed that direct the synthesis of fusion proteins containing a stretch of six histidine residues (His sub(6)) at either the N or C terminus. The His sub(6) tag allows the rapid enrichment of proteins by metal chelate affinity chro...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Gene 1994-01, Vol.147 (1), p.91-94
Hauptverfasser:	Schoen, U, Schumann, W
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus stearothermophilus Bacillus subtilis
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!