Evaluation of cytotoxicity and antiviral activity of ivermectin against Newcastle disease virus
Cytotoxic and antiviral potential of ivermectin and ribavirin was evaluated. Cytotoxicity was checked on chick primary fibroblast cell line through MTT assay. Antiviral potential was determined against Newcastle disease virus on 9-day old chicken embryos. Six different concentrations (200, 100, 50,...
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| Veröffentlicht in: | Pakistan journal of pharmaceutical sciences 2015-03, Vol.28 (2), p.597-602 |
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| container_title | Pakistan journal of pharmaceutical sciences |
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| creator | Azeem, Sidra Ashraf, Muhammad Rasheed, Muhammad Adil Anjum, Aftab Ahmad Hameed, Rabia |
| description | Cytotoxic and antiviral potential of ivermectin and ribavirin was evaluated. Cytotoxicity was checked on chick primary fibroblast cell line through MTT assay. Antiviral potential was determined against Newcastle disease virus on 9-day old chicken embryos. Six different concentrations (200, 100, 50, 25, 12.5 and 6.25μg/ml) of both the drugs were evaluated. The 100μg/ml concentration of ivermectin and higher were cytotoxic. The 25μg/ml concentration of ribavirin and higher were cytotoxic. Comparison of ivermectin and ribavirin showed that ivermectin was safe at 50μg/ml and lower concentrations. Ribavirin was protective for cell at 12.5μg/ml and 6.25μg/ml only. Comparison of antiviral activity indicated that ivermectin has strong antiviral potential at 100μg/ml and higher but same concentrations were cytotoxic. Ribavirin showed strong antiviral potential at all concentrations. |
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Cytotoxicity was checked on chick primary fibroblast cell line through MTT assay. Antiviral potential was determined against Newcastle disease virus on 9-day old chicken embryos. Six different concentrations (200, 100, 50, 25, 12.5 and 6.25μg/ml) of both the drugs were evaluated. The 100μg/ml concentration of ivermectin and higher were cytotoxic. The 25μg/ml concentration of ribavirin and higher were cytotoxic. Comparison of ivermectin and ribavirin showed that ivermectin was safe at 50μg/ml and lower concentrations. Ribavirin was protective for cell at 12.5μg/ml and 6.25μg/ml only. Comparison of antiviral activity indicated that ivermectin has strong antiviral potential at 100μg/ml and higher but same concentrations were cytotoxic. 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Cytotoxicity was checked on chick primary fibroblast cell line through MTT assay. Antiviral potential was determined against Newcastle disease virus on 9-day old chicken embryos. Six different concentrations (200, 100, 50, 25, 12.5 and 6.25μg/ml) of both the drugs were evaluated. The 100μg/ml concentration of ivermectin and higher were cytotoxic. The 25μg/ml concentration of ribavirin and higher were cytotoxic. Comparison of ivermectin and ribavirin showed that ivermectin was safe at 50μg/ml and lower concentrations. Ribavirin was protective for cell at 12.5μg/ml and 6.25μg/ml only. Comparison of antiviral activity indicated that ivermectin has strong antiviral potential at 100μg/ml and higher but same concentrations were cytotoxic. Ribavirin showed strong antiviral potential at all concentrations.</description><subject>Analysis</subject><subject>Animals</subject><subject>Antiviral agents</subject><subject>Antiviral Agents - pharmacology</subject><subject>Care and treatment</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>Chick Embryo</subject><subject>Dosage and administration</subject><subject>Ivermectin</subject><subject>Ivermectin - pharmacology</subject><subject>Newcastle disease</subject><subject>Newcastle disease virus - drug effects</subject><subject>Ribavirin - pharmacology</subject><issn>1011-601X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkFFLwzAQx_OguDn9ChLwxZdK0rRp8zjGdMLQFwXfwjW9jkjbzCad7tubsfkgyHHc8ef3vzvujEw54zyRjL9PyKX3H4zJTCl1QSZpXghWcjElermDdoRgXU9dQ80-uOC-rbFhT6GvYwa7swO0FMyhi3LE7A6HDqPQU9iA7X2gz_hlwIcWaW09gkcabaO_IucNtB6vT3VG3h6Wr4tVsn55fFrM18kmLcqQ8BJk2piilrxMjVKGsVSKQkEGPMuZQgFcKlHnDQclK1FxRANVLiqJJc8rMSN3x7nbwX2O6IPurDfYttCjG73mUjKZ56UqI3p7RDfQorZ948IA5oDrecZUlsbNaaTu_6Fi1NhZ43psbNT_GG5OF4xVh7XeDraDYa9_fy1-ALPOeb0</recordid><startdate>201503</startdate><enddate>201503</enddate><creator>Azeem, Sidra</creator><creator>Ashraf, Muhammad</creator><creator>Rasheed, Muhammad Adil</creator><creator>Anjum, Aftab Ahmad</creator><creator>Hameed, Rabia</creator><general>Pakistan Journal of Pharmaceutical Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201503</creationdate><title>Evaluation of cytotoxicity and antiviral activity of ivermectin against Newcastle disease virus</title><author>Azeem, Sidra ; Ashraf, Muhammad ; Rasheed, Muhammad Adil ; Anjum, Aftab Ahmad ; Hameed, Rabia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g278t-18a62fc7d6182c99c0026379a4a14509e3a1693d5f1a96b3b1eecab53b6e815b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Antiviral agents</topic><topic>Antiviral Agents - pharmacology</topic><topic>Care and treatment</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>Chick Embryo</topic><topic>Dosage and administration</topic><topic>Ivermectin</topic><topic>Ivermectin - pharmacology</topic><topic>Newcastle disease</topic><topic>Newcastle disease virus - drug effects</topic><topic>Ribavirin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Azeem, Sidra</creatorcontrib><creatorcontrib>Ashraf, Muhammad</creatorcontrib><creatorcontrib>Rasheed, Muhammad Adil</creatorcontrib><creatorcontrib>Anjum, Aftab Ahmad</creatorcontrib><creatorcontrib>Hameed, Rabia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Pakistan journal of pharmaceutical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Azeem, Sidra</au><au>Ashraf, Muhammad</au><au>Rasheed, Muhammad Adil</au><au>Anjum, Aftab Ahmad</au><au>Hameed, Rabia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of cytotoxicity and antiviral activity of ivermectin against Newcastle disease virus</atitle><jtitle>Pakistan journal of pharmaceutical sciences</jtitle><addtitle>Pak J Pharm Sci</addtitle><date>2015-03</date><risdate>2015</risdate><volume>28</volume><issue>2</issue><spage>597</spage><epage>602</epage><pages>597-602</pages><issn>1011-601X</issn><abstract>Cytotoxic and antiviral potential of ivermectin and ribavirin was evaluated. Cytotoxicity was checked on chick primary fibroblast cell line through MTT assay. Antiviral potential was determined against Newcastle disease virus on 9-day old chicken embryos. Six different concentrations (200, 100, 50, 25, 12.5 and 6.25μg/ml) of both the drugs were evaluated. The 100μg/ml concentration of ivermectin and higher were cytotoxic. The 25μg/ml concentration of ribavirin and higher were cytotoxic. Comparison of ivermectin and ribavirin showed that ivermectin was safe at 50μg/ml and lower concentrations. Ribavirin was protective for cell at 12.5μg/ml and 6.25μg/ml only. Comparison of antiviral activity indicated that ivermectin has strong antiviral potential at 100μg/ml and higher but same concentrations were cytotoxic. Ribavirin showed strong antiviral potential at all concentrations.</abstract><cop>Pakistan</cop><pub>Pakistan Journal of Pharmaceutical Sciences</pub><pmid>25730813</pmid><tpages>6</tpages></addata></record> |
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| ispartof | Pakistan journal of pharmaceutical sciences, 2015-03, Vol.28 (2), p.597-602 |
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| subjects | Analysis Animals Antiviral agents Antiviral Agents - pharmacology Care and treatment Cell Line Cell Survival - drug effects Chick Embryo Dosage and administration Ivermectin Ivermectin - pharmacology Newcastle disease Newcastle disease virus - drug effects Ribavirin - pharmacology |
| title | Evaluation of cytotoxicity and antiviral activity of ivermectin against Newcastle disease virus |
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