Experimental evidence suggesting that H2O2 is produced within the thylakoid membrane in a reaction between plastoquinol and singlet oxygen
•Singlet oxygen oxidizes plastoquinol to plastoquinone.•Oxidation of plastoquinol by singlet oxygen produces hydrogen peroxide.•Hydrogen peroxide formation is stoichiometric with oxidation of plastoquinol.•Plastohydroquinone enhances the chemiluminescence of the luminol-peroxidase system. Plastoquin...
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| Veröffentlicht in: | FEBS letters 2015-03, Vol.589 (6), p.779-786 |
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| creator | Khorobrykh, Sergey A. Karonen, Maarit Tyystjärvi, Esa |
| description | •Singlet oxygen oxidizes plastoquinol to plastoquinone.•Oxidation of plastoquinol by singlet oxygen produces hydrogen peroxide.•Hydrogen peroxide formation is stoichiometric with oxidation of plastoquinol.•Plastohydroquinone enhances the chemiluminescence of the luminol-peroxidase system.
Plastoquinol (PQH2-9) and plastoquinone (PQ-9) mediate photosynthetic electron transfer. We isolated PQH2-9 from thylakoid membranes, purified it with HPLC, subjected the purified PQH2-9 to singlet oxygen (1O2) and analyzed the products. The main reaction of 1O2 with PQH2-9 in methanol was found to result in formation of PQ-9 and H2O2, and the amount of H2O2 produced was essentially the same as the amount of oxidized PQH2-9. Formation of H2O2 in the reaction between 1O2 and PQH2-9 may be an important source of H2O2 within the lipophilic thylakoid membrane. |
| doi_str_mv | 10.1016/j.febslet.2015.02.011 |
| format | Article |
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Plastoquinol (PQH2-9) and plastoquinone (PQ-9) mediate photosynthetic electron transfer. We isolated PQH2-9 from thylakoid membranes, purified it with HPLC, subjected the purified PQH2-9 to singlet oxygen (1O2) and analyzed the products. The main reaction of 1O2 with PQH2-9 in methanol was found to result in formation of PQ-9 and H2O2, and the amount of H2O2 produced was essentially the same as the amount of oxidized PQH2-9. Formation of H2O2 in the reaction between 1O2 and PQH2-9 may be an important source of H2O2 within the lipophilic thylakoid membrane.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2015.02.011</identifier><identifier>PMID: 25701589</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>1O2 ; 2-iodo-6-isopropyl-3-methyl-2′,4,4′-trinitrodiphenyl ether ; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ; Chl ; chlorophyll ; Cucurbita - cytology ; Cucurbita - metabolism ; DNP-INT ; HEPES ; HO2 ; Hydrogen peroxide ; Hydrogen Peroxide - metabolism ; hydroperoxyl radical ; MeOH ; methanol ; O2 ; Oxidation-Reduction ; P680 ; Pheo ; pheophytin a—primary electron acceptor of PSII ; Photosystem II ; photosystems I and II, respectively ; Plant Leaves - cytology ; Plant Leaves - metabolism ; plastoquinol and plastoquinone, respectively ; Plastoquinone ; Plastoquinone - analogs & derivatives ; Plastoquinone - metabolism ; plastosemiquinone radical and plastosemiquinone anion radical, respectively ; PQH-9 and PQ−-9 ; PQH2-9 and PQ-9 ; primary electron donor of PSII ; PSI and PSII ; reactive oxygen species ; ROS ; Singlet oxygen ; Singlet Oxygen - metabolism ; superoxide anion radical ; Thylakoid membrane ; Thylakoids - metabolism ; ubiquinol-9 ; UQH2-9</subject><ispartof>FEBS letters, 2015-03, Vol.589 (6), p.779-786</ispartof><rights>2015 Federation of European Biochemical Societies</rights><rights>FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2015.02.011$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014579315000915$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,1427,3537,27901,27902,45550,45551,46384,46808,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25701589$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khorobrykh, Sergey A.</creatorcontrib><creatorcontrib>Karonen, Maarit</creatorcontrib><creatorcontrib>Tyystjärvi, Esa</creatorcontrib><title>Experimental evidence suggesting that H2O2 is produced within the thylakoid membrane in a reaction between plastoquinol and singlet oxygen</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>•Singlet oxygen oxidizes plastoquinol to plastoquinone.•Oxidation of plastoquinol by singlet oxygen produces hydrogen peroxide.•Hydrogen peroxide formation is stoichiometric with oxidation of plastoquinol.•Plastohydroquinone enhances the chemiluminescence of the luminol-peroxidase system.
Plastoquinol (PQH2-9) and plastoquinone (PQ-9) mediate photosynthetic electron transfer. We isolated PQH2-9 from thylakoid membranes, purified it with HPLC, subjected the purified PQH2-9 to singlet oxygen (1O2) and analyzed the products. The main reaction of 1O2 with PQH2-9 in methanol was found to result in formation of PQ-9 and H2O2, and the amount of H2O2 produced was essentially the same as the amount of oxidized PQH2-9. Formation of H2O2 in the reaction between 1O2 and PQH2-9 may be an important source of H2O2 within the lipophilic thylakoid membrane.</description><subject>1O2</subject><subject>2-iodo-6-isopropyl-3-methyl-2′,4,4′-trinitrodiphenyl ether</subject><subject>4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid</subject><subject>Chl</subject><subject>chlorophyll</subject><subject>Cucurbita - cytology</subject><subject>Cucurbita - metabolism</subject><subject>DNP-INT</subject><subject>HEPES</subject><subject>HO2</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>hydroperoxyl radical</subject><subject>MeOH</subject><subject>methanol</subject><subject>O2</subject><subject>Oxidation-Reduction</subject><subject>P680</subject><subject>Pheo</subject><subject>pheophytin a—primary electron acceptor of PSII</subject><subject>Photosystem II</subject><subject>photosystems I and II, respectively</subject><subject>Plant Leaves - cytology</subject><subject>Plant Leaves - metabolism</subject><subject>plastoquinol and plastoquinone, respectively</subject><subject>Plastoquinone</subject><subject>Plastoquinone - analogs & derivatives</subject><subject>Plastoquinone - metabolism</subject><subject>plastosemiquinone radical and plastosemiquinone anion radical, respectively</subject><subject>PQH-9 and PQ−-9</subject><subject>PQH2-9 and PQ-9</subject><subject>primary electron donor of PSII</subject><subject>PSI and PSII</subject><subject>reactive oxygen species</subject><subject>ROS</subject><subject>Singlet oxygen</subject><subject>Singlet Oxygen - metabolism</subject><subject>superoxide anion radical</subject><subject>Thylakoid membrane</subject><subject>Thylakoids - metabolism</subject><subject>ubiquinol-9</subject><subject>UQH2-9</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhi0EokvhEUA-cklqO44TnxBUWxapUg_A2XLsydaL42xjp9t9hT41jnbpkYM1suf3r_nnQ-gjJSUlVFztyh666CGVjNC6JKwklL5CK9o2VVFx0b5GK0IoL-pGVhfoXYw7ku8tlW_RBaub_KmVK_S8ftrD5AYISXsMj85CMIDjvN1CTC5scbrXCW_YHcMu4v002tmAxQeX7l3ITcjn6PWf0Vk8wNBNOgDOHY0n0Ca5MeAO0gEg4L3XMY0PswujxzpYHLN_ToDHp-MWwnv0ptc-wodzvUS_b9a_rjfF7d33H9dfbwvgXNaFaRvbG2alAF4RoUVtTK6tNVI2upVAaNW3jbZWWNNJ0rN6eeWV1Q1vCFSX6PPJN4d5mHNKNbhowPs8-ThHRYUgnHNKRJZ-OkvnbgCr9nlTejqqf_vLgs1JcHAeji99StQCSe3UGZJaICnCVIakbtbf2M-FzYKG1hmLpHW2-nKygpz90cGkonELDOsmMEnZ0f3ft_oLcl6iaA</recordid><startdate>20150312</startdate><enddate>20150312</enddate><creator>Khorobrykh, Sergey A.</creator><creator>Karonen, Maarit</creator><creator>Tyystjärvi, Esa</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20150312</creationdate><title>Experimental evidence suggesting that H2O2 is produced within the thylakoid membrane in a reaction between plastoquinol and singlet oxygen</title><author>Khorobrykh, Sergey A. ; Karonen, Maarit ; Tyystjärvi, Esa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e4495-c87dfc2d96e4306a65cc3068dc997a89e013f87add6dcb90f25a89e43da7470e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>1O2</topic><topic>2-iodo-6-isopropyl-3-methyl-2′,4,4′-trinitrodiphenyl ether</topic><topic>4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid</topic><topic>Chl</topic><topic>chlorophyll</topic><topic>Cucurbita - cytology</topic><topic>Cucurbita - metabolism</topic><topic>DNP-INT</topic><topic>HEPES</topic><topic>HO2</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>hydroperoxyl radical</topic><topic>MeOH</topic><topic>methanol</topic><topic>O2</topic><topic>Oxidation-Reduction</topic><topic>P680</topic><topic>Pheo</topic><topic>pheophytin a—primary electron acceptor of PSII</topic><topic>Photosystem II</topic><topic>photosystems I and II, respectively</topic><topic>Plant Leaves - cytology</topic><topic>Plant Leaves - metabolism</topic><topic>plastoquinol and plastoquinone, respectively</topic><topic>Plastoquinone</topic><topic>Plastoquinone - analogs & derivatives</topic><topic>Plastoquinone - metabolism</topic><topic>plastosemiquinone radical and plastosemiquinone anion radical, respectively</topic><topic>PQH-9 and PQ−-9</topic><topic>PQH2-9 and PQ-9</topic><topic>primary electron donor of PSII</topic><topic>PSI and PSII</topic><topic>reactive oxygen species</topic><topic>ROS</topic><topic>Singlet oxygen</topic><topic>Singlet Oxygen - metabolism</topic><topic>superoxide anion radical</topic><topic>Thylakoid membrane</topic><topic>Thylakoids - metabolism</topic><topic>ubiquinol-9</topic><topic>UQH2-9</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khorobrykh, Sergey A.</creatorcontrib><creatorcontrib>Karonen, Maarit</creatorcontrib><creatorcontrib>Tyystjärvi, Esa</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khorobrykh, Sergey A.</au><au>Karonen, Maarit</au><au>Tyystjärvi, Esa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Experimental evidence suggesting that H2O2 is produced within the thylakoid membrane in a reaction between plastoquinol and singlet oxygen</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2015-03-12</date><risdate>2015</risdate><volume>589</volume><issue>6</issue><spage>779</spage><epage>786</epage><pages>779-786</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>•Singlet oxygen oxidizes plastoquinol to plastoquinone.•Oxidation of plastoquinol by singlet oxygen produces hydrogen peroxide.•Hydrogen peroxide formation is stoichiometric with oxidation of plastoquinol.•Plastohydroquinone enhances the chemiluminescence of the luminol-peroxidase system.
Plastoquinol (PQH2-9) and plastoquinone (PQ-9) mediate photosynthetic electron transfer. We isolated PQH2-9 from thylakoid membranes, purified it with HPLC, subjected the purified PQH2-9 to singlet oxygen (1O2) and analyzed the products. The main reaction of 1O2 with PQH2-9 in methanol was found to result in formation of PQ-9 and H2O2, and the amount of H2O2 produced was essentially the same as the amount of oxidized PQH2-9. Formation of H2O2 in the reaction between 1O2 and PQH2-9 may be an important source of H2O2 within the lipophilic thylakoid membrane.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>25701589</pmid><doi>10.1016/j.febslet.2015.02.011</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Free Content; MEDLINE; Elsevier ScienceDirect Journals Complete; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | 1O2 2-iodo-6-isopropyl-3-methyl-2′,4,4′-trinitrodiphenyl ether 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid Chl chlorophyll Cucurbita - cytology Cucurbita - metabolism DNP-INT HEPES HO2 Hydrogen peroxide Hydrogen Peroxide - metabolism hydroperoxyl radical MeOH methanol O2 Oxidation-Reduction P680 Pheo pheophytin a—primary electron acceptor of PSII Photosystem II photosystems I and II, respectively Plant Leaves - cytology Plant Leaves - metabolism plastoquinol and plastoquinone, respectively Plastoquinone Plastoquinone - analogs & derivatives Plastoquinone - metabolism plastosemiquinone radical and plastosemiquinone anion radical, respectively PQH-9 and PQ−-9 PQH2-9 and PQ-9 primary electron donor of PSII PSI and PSII reactive oxygen species ROS Singlet oxygen Singlet Oxygen - metabolism superoxide anion radical Thylakoid membrane Thylakoids - metabolism ubiquinol-9 UQH2-9 |
| title | Experimental evidence suggesting that H2O2 is produced within the thylakoid membrane in a reaction between plastoquinol and singlet oxygen |
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