Algicidal activity of Bacillus sp. Lzh-5 and its algicidal compounds against Microcystis aeruginosa
A freshwater algicidal bacterial strain, Lzh-5, isolated from Lake Taihu, with strong algicidal activity against Microcystis aeruginosa, was identified as Bacillus sp. based on its phenotypic characteristics and 16S ribosomal RNA (rRNA) gene sequence. The algicidal mode of Bacillus sp. Lzh-5 was ind...
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| creator | Li, Zhenghua Geng, Mengxin Yang, Hong |
| description | A freshwater algicidal bacterial strain, Lzh-5, isolated from Lake Taihu, with strong algicidal activity against Microcystis aeruginosa, was identified as Bacillus sp. based on its phenotypic characteristics and 16S ribosomal RNA (rRNA) gene sequence. The algicidal mode of Bacillus sp. Lzh-5 was indirect, attacking M. aeruginosa cells by releasing algicidal compounds. Two algicidal compounds (S-5A and S-5B) produced by Bacillus sp. Lzh-5 were purified with ethyl acetate extraction, column chromatography, and high-performance liquid chromatography and identified as hexahydropyrrolo[1,2-a]pyrazine-1,4-dione and 3-isopropyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione based on liquid chromatography–mass spectrometry, gas chromatography–mass spectrometry, and nuclear magnetic resonance analyses. The active algicidal compounds S-5A (hexahydropyrrolo[1,2-a]pyrazine-1,4-dione) and S-5B (3-isopropyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione) displayed high levels of algicidal activity against M. aeruginosa 9110, with LD₅₀values of 5.7 and 19.4 μg/ml, respectively. This is the first report of 3-isopropyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione as an algicidal compound. Compounds S-5A and S-5B also induced obvious morphological changes in M. aeruginosa 9110. In cocultures of M. aeruginosa 9110 and Bacillus sp. Lzh-5, the cell density of Bacillus sp. Lzh-5 and the concentrations of S-5A and S-5B correlated positively with the algicidal activity. Our results indicate that strain Lzh-5 and its two algicidal compounds are potentially useful for controlling cyanobacterial blooms in Lake Taihu. |
| doi_str_mv | 10.1007/s00253-014-6043-6 |
| format | Article |
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Lzh-5 and its algicidal compounds against Microcystis aeruginosa</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Li, Zhenghua ; Geng, Mengxin ; Yang, Hong</creator><creatorcontrib>Li, Zhenghua ; Geng, Mengxin ; Yang, Hong</creatorcontrib><description>A freshwater algicidal bacterial strain, Lzh-5, isolated from Lake Taihu, with strong algicidal activity against Microcystis aeruginosa, was identified as Bacillus sp. based on its phenotypic characteristics and 16S ribosomal RNA (rRNA) gene sequence. The algicidal mode of Bacillus sp. Lzh-5 was indirect, attacking M. aeruginosa cells by releasing algicidal compounds. Two algicidal compounds (S-5A and S-5B) produced by Bacillus sp. Lzh-5 were purified with ethyl acetate extraction, column chromatography, and high-performance liquid chromatography and identified as hexahydropyrrolo[1,2-a]pyrazine-1,4-dione and 3-isopropyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione based on liquid chromatography–mass spectrometry, gas chromatography–mass spectrometry, and nuclear magnetic resonance analyses. The active algicidal compounds S-5A (hexahydropyrrolo[1,2-a]pyrazine-1,4-dione) and S-5B (3-isopropyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione) displayed high levels of algicidal activity against M. aeruginosa 9110, with LD₅₀values of 5.7 and 19.4 μg/ml, respectively. This is the first report of 3-isopropyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione as an algicidal compound. Compounds S-5A and S-5B also induced obvious morphological changes in M. aeruginosa 9110. In cocultures of M. aeruginosa 9110 and Bacillus sp. Lzh-5, the cell density of Bacillus sp. Lzh-5 and the concentrations of S-5A and S-5B correlated positively with the algicidal activity. Our results indicate that strain Lzh-5 and its two algicidal compounds are potentially useful for controlling cyanobacterial blooms in Lake Taihu.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-014-6043-6</identifier><identifier>PMID: 25196270</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Algae ; Antibiosis - physiology ; Bacillus ; Bacillus (bacteria) ; Bacillus - genetics ; Bacillus - metabolism ; Bacteria ; Biocides ; Biological Control Agents ; Biomedical and Life Sciences ; Biotechnology ; Chemical properties ; Chromatography ; Chromatography, Liquid ; Coculture Techniques ; Control ; correlation ; DNA, Bacterial - genetics ; Environmental Biotechnology ; ethyl acetate ; Eutrophication ; freshwater ; Gas chromatography ; Gas Chromatography-Mass Spectrometry ; Genetic aspects ; Health aspects ; High performance liquid chromatography ; Identification and classification ; Laboratories ; Lakes ; Lakes - microbiology ; Lethal Dose 50 ; Life Sciences ; Liquid chromatography ; Mass spectrometry ; Methods ; Microbial Genetics and Genomics ; Microbiology ; Microcystis ; Microcystis - drug effects ; Microcystis aeruginosa ; NMR ; Nuclear magnetic resonance ; nuclear magnetic resonance spectroscopy ; nucleotide sequences ; Physiological aspects ; Pollution control ; Prevention ; ribosomal RNA ; RNA sequencing ; RNA, Ribosomal, 16S - genetics ; Scientific imaging ; Sequence Analysis, DNA ; Studies ; Water analysis</subject><ispartof>Applied microbiology and biotechnology, 2015-01, Vol.99 (2), p.981-990</ispartof><rights>Springer-Verlag Berlin Heidelberg 2014</rights><rights>COPYRIGHT 2015 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c668t-1697cd9b67a19e76f1ebaa68cdb4e00ae365128aba56458426778512bb62ed5c3</citedby><cites>FETCH-LOGICAL-c668t-1697cd9b67a19e76f1ebaa68cdb4e00ae365128aba56458426778512bb62ed5c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-014-6043-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-014-6043-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25196270$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Zhenghua</creatorcontrib><creatorcontrib>Geng, Mengxin</creatorcontrib><creatorcontrib>Yang, Hong</creatorcontrib><title>Algicidal activity of Bacillus sp. Lzh-5 and its algicidal compounds against Microcystis aeruginosa</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>A freshwater algicidal bacterial strain, Lzh-5, isolated from Lake Taihu, with strong algicidal activity against Microcystis aeruginosa, was identified as Bacillus sp. based on its phenotypic characteristics and 16S ribosomal RNA (rRNA) gene sequence. The algicidal mode of Bacillus sp. Lzh-5 was indirect, attacking M. aeruginosa cells by releasing algicidal compounds. Two algicidal compounds (S-5A and S-5B) produced by Bacillus sp. Lzh-5 were purified with ethyl acetate extraction, column chromatography, and high-performance liquid chromatography and identified as hexahydropyrrolo[1,2-a]pyrazine-1,4-dione and 3-isopropyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione based on liquid chromatography–mass spectrometry, gas chromatography–mass spectrometry, and nuclear magnetic resonance analyses. The active algicidal compounds S-5A (hexahydropyrrolo[1,2-a]pyrazine-1,4-dione) and S-5B (3-isopropyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione) displayed high levels of algicidal activity against M. aeruginosa 9110, with LD₅₀values of 5.7 and 19.4 μg/ml, respectively. This is the first report of 3-isopropyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione as an algicidal compound. Compounds S-5A and S-5B also induced obvious morphological changes in M. aeruginosa 9110. In cocultures of M. aeruginosa 9110 and Bacillus sp. Lzh-5, the cell density of Bacillus sp. Lzh-5 and the concentrations of S-5A and S-5B correlated positively with the algicidal activity. Our results indicate that strain Lzh-5 and its two algicidal compounds are potentially useful for controlling cyanobacterial blooms in Lake Taihu.</description><subject>Algae</subject><subject>Antibiosis - physiology</subject><subject>Bacillus</subject><subject>Bacillus (bacteria)</subject><subject>Bacillus - genetics</subject><subject>Bacillus - metabolism</subject><subject>Bacteria</subject><subject>Biocides</subject><subject>Biological Control Agents</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Chemical properties</subject><subject>Chromatography</subject><subject>Chromatography, Liquid</subject><subject>Coculture Techniques</subject><subject>Control</subject><subject>correlation</subject><subject>DNA, Bacterial - genetics</subject><subject>Environmental Biotechnology</subject><subject>ethyl acetate</subject><subject>Eutrophication</subject><subject>freshwater</subject><subject>Gas chromatography</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>High performance liquid chromatography</subject><subject>Identification and classification</subject><subject>Laboratories</subject><subject>Lakes</subject><subject>Lakes - microbiology</subject><subject>Lethal Dose 50</subject><subject>Life Sciences</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Methods</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Microcystis</subject><subject>Microcystis - drug effects</subject><subject>Microcystis aeruginosa</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>nuclear magnetic resonance spectroscopy</subject><subject>nucleotide sequences</subject><subject>Physiological aspects</subject><subject>Pollution control</subject><subject>Prevention</subject><subject>ribosomal RNA</subject><subject>RNA sequencing</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Scientific imaging</subject><subject>Sequence Analysis, DNA</subject><subject>Studies</subject><subject>Water analysis</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNk09v1DAQxSMEokvhA3CBSFzgkMV27HFyXCr-VFqEROnZmjhOcJXEi50glk-Po5Syi1CpfLA0_r1nzfg5SZ5SsqaEyNeBECbyjFCeAeF5BveSFeU5ywhQfj9ZESpFJkVZnCSPQrgihLIC4GFywgQtgUmySvSma622NXYp6tF-t-M-dU36BrXtuimkYbdOtz-_ZiLFoU7tGFK8EWjX79w01LHWoh3CmH602ju9D6ONNeOn1g4u4OPkQYNdME-u99Pk8t3bL2cfsu2n9-dnm22mAYoxo1BKXZcVSKSlkdBQUyFCoeuKG0LQ5CBiA1ihAC4KzkDKIlaqCpiphc5Pk5eL7867b5MJo-pt0KbrcDBuCorCPKWSkfIOqGCcUQkioi_-Qq_c5IfYSKR4wXIqJfyhWuyMskPjRo96NlUbLgFKKEp5O0WJIKKUPFLrf1Bx1aa32g2msbF-ZHs3wcENr44EkRnNj7HFKQR1fvH52Py_7IEvXdgYgxC8adTO2x79XlGi5siqJbIqRlbNj6HmyT27nu9U9aa-UfzOaATYAoR4NLTGHzzALa7PF1GDTmHrbVCXF4xQET8BF6IQ-S9pTvks</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Li, Zhenghua</creator><creator>Geng, Mengxin</creator><creator>Yang, Hong</creator><general>Springer-Verlag</general><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>20150101</creationdate><title>Algicidal activity of Bacillus sp. Lzh-5 and its algicidal compounds against Microcystis aeruginosa</title><author>Li, Zhenghua ; Geng, Mengxin ; Yang, Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c668t-1697cd9b67a19e76f1ebaa68cdb4e00ae365128aba56458426778512bb62ed5c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Algae</topic><topic>Antibiosis - physiology</topic><topic>Bacillus</topic><topic>Bacillus (bacteria)</topic><topic>Bacillus - genetics</topic><topic>Bacillus - metabolism</topic><topic>Bacteria</topic><topic>Biocides</topic><topic>Biological Control Agents</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Chemical properties</topic><topic>Chromatography</topic><topic>Chromatography, Liquid</topic><topic>Coculture Techniques</topic><topic>Control</topic><topic>correlation</topic><topic>DNA, Bacterial - genetics</topic><topic>Environmental Biotechnology</topic><topic>ethyl acetate</topic><topic>Eutrophication</topic><topic>freshwater</topic><topic>Gas chromatography</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>High performance liquid chromatography</topic><topic>Identification and classification</topic><topic>Laboratories</topic><topic>Lakes</topic><topic>Lakes - microbiology</topic><topic>Lethal Dose 50</topic><topic>Life Sciences</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Methods</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Microcystis</topic><topic>Microcystis - drug effects</topic><topic>Microcystis aeruginosa</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>nuclear magnetic resonance spectroscopy</topic><topic>nucleotide sequences</topic><topic>Physiological aspects</topic><topic>Pollution control</topic><topic>Prevention</topic><topic>ribosomal RNA</topic><topic>RNA sequencing</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Scientific imaging</topic><topic>Sequence Analysis, DNA</topic><topic>Studies</topic><topic>Water analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Zhenghua</creatorcontrib><creatorcontrib>Geng, Mengxin</creatorcontrib><creatorcontrib>Yang, Hong</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Zhenghua</au><au>Geng, Mengxin</au><au>Yang, Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Algicidal activity of Bacillus sp. Lzh-5 and its algicidal compounds against Microcystis aeruginosa</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>99</volume><issue>2</issue><spage>981</spage><epage>990</epage><pages>981-990</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><abstract>A freshwater algicidal bacterial strain, Lzh-5, isolated from Lake Taihu, with strong algicidal activity against Microcystis aeruginosa, was identified as Bacillus sp. based on its phenotypic characteristics and 16S ribosomal RNA (rRNA) gene sequence. The algicidal mode of Bacillus sp. Lzh-5 was indirect, attacking M. aeruginosa cells by releasing algicidal compounds. Two algicidal compounds (S-5A and S-5B) produced by Bacillus sp. Lzh-5 were purified with ethyl acetate extraction, column chromatography, and high-performance liquid chromatography and identified as hexahydropyrrolo[1,2-a]pyrazine-1,4-dione and 3-isopropyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione based on liquid chromatography–mass spectrometry, gas chromatography–mass spectrometry, and nuclear magnetic resonance analyses. The active algicidal compounds S-5A (hexahydropyrrolo[1,2-a]pyrazine-1,4-dione) and S-5B (3-isopropyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione) displayed high levels of algicidal activity against M. aeruginosa 9110, with LD₅₀values of 5.7 and 19.4 μg/ml, respectively. This is the first report of 3-isopropyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione as an algicidal compound. Compounds S-5A and S-5B also induced obvious morphological changes in M. aeruginosa 9110. In cocultures of M. aeruginosa 9110 and Bacillus sp. Lzh-5, the cell density of Bacillus sp. Lzh-5 and the concentrations of S-5A and S-5B correlated positively with the algicidal activity. Our results indicate that strain Lzh-5 and its two algicidal compounds are potentially useful for controlling cyanobacterial blooms in Lake Taihu.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>25196270</pmid><doi>10.1007/s00253-014-6043-6</doi><tpages>10</tpages></addata></record> |
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| subjects | Algae Antibiosis - physiology Bacillus Bacillus (bacteria) Bacillus - genetics Bacillus - metabolism Bacteria Biocides Biological Control Agents Biomedical and Life Sciences Biotechnology Chemical properties Chromatography Chromatography, Liquid Coculture Techniques Control correlation DNA, Bacterial - genetics Environmental Biotechnology ethyl acetate Eutrophication freshwater Gas chromatography Gas Chromatography-Mass Spectrometry Genetic aspects Health aspects High performance liquid chromatography Identification and classification Laboratories Lakes Lakes - microbiology Lethal Dose 50 Life Sciences Liquid chromatography Mass spectrometry Methods Microbial Genetics and Genomics Microbiology Microcystis Microcystis - drug effects Microcystis aeruginosa NMR Nuclear magnetic resonance nuclear magnetic resonance spectroscopy nucleotide sequences Physiological aspects Pollution control Prevention ribosomal RNA RNA sequencing RNA, Ribosomal, 16S - genetics Scientific imaging Sequence Analysis, DNA Studies Water analysis |
| title | Algicidal activity of Bacillus sp. Lzh-5 and its algicidal compounds against Microcystis aeruginosa |
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