Status of benzimidazole resistance in Haemonchus contortus of goats from different geographic regions of Uttar Pradesh, India

•A comprehensive survey has been done for BZ resistance in H. contortus by FECRT and AS-PCR.•FECRT showed BZ resistance prevalent in all regions; however, six farms harboured susceptible populations.•It revealed 55–85% of H. contortus were homozygous resistant and 5–24% heterozygous.•The allele freq...

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Veröffentlicht in:Veterinary parasitology 2015-03, Vol.208 (3-4), p.263-267
Hauptverfasser: Chandra, S., Prasad, A., Yadav, N., Latchumikanthan, A., Rakesh, R.L., Praveen, K., Khobra, V., Subramani, K.V., Misri, J., Sankar, M.
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container_end_page 267
container_issue 3-4
container_start_page 263
container_title Veterinary parasitology
container_volume 208
creator Chandra, S.
Prasad, A.
Yadav, N.
Latchumikanthan, A.
Rakesh, R.L.
Praveen, K.
Khobra, V.
Subramani, K.V.
Misri, J.
Sankar, M.
description •A comprehensive survey has been done for BZ resistance in H. contortus by FECRT and AS-PCR.•FECRT showed BZ resistance prevalent in all regions; however, six farms harboured susceptible populations.•It revealed 55–85% of H. contortus were homozygous resistant and 5–24% heterozygous.•The allele frequencies were 67–87.5% for resistant and 12.5–33% for susceptible.•The survey indicated that the status of BZ resistance is in alarming conditions. Present study was designed to survey the status of benzimidazole (BZ) resistance in goat flocks of different agro climatic regions of Uttar Pradesh (U.P.), India by faecal egg count reduction test (FECRT) and allele specific PCR (AS-PCR). Faecal egg counts and larval culture were made from representative samples of all regions. The results of the faecal culture and PCR-RFLP on beta tubulin isotype-1 gene showed Haemonchus contortus was predominant species. FECRT results showed BZ resistance prevalent in all regions. However, six farms of twenty screened, harboured susceptible populations of strongyles. Three hundred H. contortus infective larvae from all regions of the state were genotyped for BZ resistance. AS-PCR results revealed 55–85% of H. contortus homozygous resistant (rr), 10–21% homozygous susceptible (SS) and 5–24% heterozygous (rS) among different regions of U.P. The allele frequencies were 67–87.5% for resistant (TTC) and 12.5–33% for susceptible (TAC). The survey indicated that the status of BZ resistance is in alarming conditions in all the parts of the state.
doi_str_mv 10.1016/j.vetpar.2015.01.005
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Present study was designed to survey the status of benzimidazole (BZ) resistance in goat flocks of different agro climatic regions of Uttar Pradesh (U.P.), India by faecal egg count reduction test (FECRT) and allele specific PCR (AS-PCR). Faecal egg counts and larval culture were made from representative samples of all regions. The results of the faecal culture and PCR-RFLP on beta tubulin isotype-1 gene showed Haemonchus contortus was predominant species. FECRT results showed BZ resistance prevalent in all regions. However, six farms of twenty screened, harboured susceptible populations of strongyles. Three hundred H. contortus infective larvae from all regions of the state were genotyped for BZ resistance. AS-PCR results revealed 55–85% of H. contortus homozygous resistant (rr), 10–21% homozygous susceptible (SS) and 5–24% heterozygous (rS) among different regions of U.P. The allele frequencies were 67–87.5% for resistant (TTC) and 12.5–33% for susceptible (TAC). 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Present study was designed to survey the status of benzimidazole (BZ) resistance in goat flocks of different agro climatic regions of Uttar Pradesh (U.P.), India by faecal egg count reduction test (FECRT) and allele specific PCR (AS-PCR). Faecal egg counts and larval culture were made from representative samples of all regions. The results of the faecal culture and PCR-RFLP on beta tubulin isotype-1 gene showed Haemonchus contortus was predominant species. FECRT results showed BZ resistance prevalent in all regions. However, six farms of twenty screened, harboured susceptible populations of strongyles. Three hundred H. contortus infective larvae from all regions of the state were genotyped for BZ resistance. AS-PCR results revealed 55–85% of H. contortus homozygous resistant (rr), 10–21% homozygous susceptible (SS) and 5–24% heterozygous (rS) among different regions of U.P. The allele frequencies were 67–87.5% for resistant (TTC) and 12.5–33% for susceptible (TAC). 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however, six farms harboured susceptible populations.•It revealed 55–85% of H. contortus were homozygous resistant and 5–24% heterozygous.•The allele frequencies were 67–87.5% for resistant and 12.5–33% for susceptible.•The survey indicated that the status of BZ resistance is in alarming conditions. Present study was designed to survey the status of benzimidazole (BZ) resistance in goat flocks of different agro climatic regions of Uttar Pradesh (U.P.), India by faecal egg count reduction test (FECRT) and allele specific PCR (AS-PCR). Faecal egg counts and larval culture were made from representative samples of all regions. The results of the faecal culture and PCR-RFLP on beta tubulin isotype-1 gene showed Haemonchus contortus was predominant species. FECRT results showed BZ resistance prevalent in all regions. However, six farms of twenty screened, harboured susceptible populations of strongyles. Three hundred H. contortus infective larvae from all regions of the state were genotyped for BZ resistance. AS-PCR results revealed 55–85% of H. contortus homozygous resistant (rr), 10–21% homozygous susceptible (SS) and 5–24% heterozygous (rS) among different regions of U.P. The allele frequencies were 67–87.5% for resistant (TTC) and 12.5–33% for susceptible (TAC). 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source MEDLINE; Elsevier ScienceDirect Journals Complete
subjects Allele specific-PCR
Animals
Benzimidazole resistance
Benzimidazoles - pharmacology
Drug Resistance
Feces - parasitology
Goat Diseases - epidemiology
Goat Diseases - parasitology
Goats
Haemonchiasis - epidemiology
Haemonchiasis - veterinary
Haemonchus - drug effects
Haemonchus contortus
India - epidemiology
β-tubulin gene
title Status of benzimidazole resistance in Haemonchus contortus of goats from different geographic regions of Uttar Pradesh, India
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