Prevalence and characteristics of Cyprinid herpesvirus 3 (CyHV-3) infection in common carp (Cyprinus carpio L.) inhabiting three rivers in Kochi Prefecture, Japan
•We detected and quantified CyHV-3 in 227 common carp from three rivers, Kochi, Japan.•CyHV-3 DNA was detected but infectious virus was not isolated in 23 tissue samples.•CyHV-3 DNA load did not differ statistically between sampling months.•All isolates showed similar genetic features to the past is...
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| Veröffentlicht in: | Veterinary microbiology 2015-02, Vol.175 (2-4), p.362-368 |
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| creator | Fujioka, Hiroya Yamasaki, Kenichi Furusawa, Keiki Tamura, Kazuki Oguro, Kazuki Kurihara, Sumire Seki, Shingo Oshima, Syun-ichirou Imajoh, Masayuki |
| description | •We detected and quantified CyHV-3 in 227 common carp from three rivers, Kochi, Japan.•CyHV-3 DNA was detected but infectious virus was not isolated in 23 tissue samples.•CyHV-3 DNA load did not differ statistically between sampling months.•All isolates showed similar genetic features to the past isolates in Japan.•We report a first finding of presence of circular or concatameric CyHV-3 DNA in carp.
Cyprinid herpesvirus 3 (CyHV-3) causes lethal disease in common and koi carp. Mortality by CyHV-3 disease has not been reported since 2011 in Kochi Prefecture, Japan. Here, we detected and quantified CyHV-3 in common carp inhabiting three rivers in the prefecture to examine if the carp are carriers of CyHV-3 as a source of infection. CyHV-3 DNA was detected in 16.7% (12/72) of brain samples in Kagami River, 3.9% (3/76) of brain and 3.9% (3/76) of gill samples in Monobe River, and 5.1% (4/79) of brain and 1.3% (1/79) of gill samples in Wajiki River. CyHV-3 genotypes identified in the 23 samples were classified as the J genotype A1 that has been found in Japan. The CyHV-3 DNA load did not differ statistically between sampling months, indicating that CyHV-3 has been silent in common carp, unlike Lake Biwa where the annual reactivation occurs in spring. Taken together, our results represented definitive evidence that seasonal changes in water temperature do not affect CyHV-3 activity in carp. Considering that infectious virus was not isolated from CyHV-3 DNA-positive samples, it was suggested that CyHV-3 establishes a latent infection in carp populations inhabiting Kagami River, Monobe River and Wajiki River. Further, the presence of circular or concatameric CyHV-3 DNA was detected in five of 23 CyHV-3 DNA-positive samples. Common carp inhabiting Lake Biwa were reported previously to harbor linear but not circular CyHV-3 DNA. This difference suggested that the CyHV-3 genome may be circularized for long-term maintenance without active viral replication. |
| doi_str_mv | 10.1016/j.vetmic.2014.12.002 |
| format | Article |
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Cyprinid herpesvirus 3 (CyHV-3) causes lethal disease in common and koi carp. Mortality by CyHV-3 disease has not been reported since 2011 in Kochi Prefecture, Japan. Here, we detected and quantified CyHV-3 in common carp inhabiting three rivers in the prefecture to examine if the carp are carriers of CyHV-3 as a source of infection. CyHV-3 DNA was detected in 16.7% (12/72) of brain samples in Kagami River, 3.9% (3/76) of brain and 3.9% (3/76) of gill samples in Monobe River, and 5.1% (4/79) of brain and 1.3% (1/79) of gill samples in Wajiki River. CyHV-3 genotypes identified in the 23 samples were classified as the J genotype A1 that has been found in Japan. The CyHV-3 DNA load did not differ statistically between sampling months, indicating that CyHV-3 has been silent in common carp, unlike Lake Biwa where the annual reactivation occurs in spring. Taken together, our results represented definitive evidence that seasonal changes in water temperature do not affect CyHV-3 activity in carp. Considering that infectious virus was not isolated from CyHV-3 DNA-positive samples, it was suggested that CyHV-3 establishes a latent infection in carp populations inhabiting Kagami River, Monobe River and Wajiki River. Further, the presence of circular or concatameric CyHV-3 DNA was detected in five of 23 CyHV-3 DNA-positive samples. Common carp inhabiting Lake Biwa were reported previously to harbor linear but not circular CyHV-3 DNA. This difference suggested that the CyHV-3 genome may be circularized for long-term maintenance without active viral replication.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2014.12.002</identifier><identifier>PMID: 25554244</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Carps ; Cyprinid herpesvirus 3 ; Cyprinus carpio ; Fish Diseases - epidemiology ; Fish Diseases - virology ; Genotyping ; Herpesviridae - isolation & purification ; Herpesviridae Infections - veterinary ; Herpesviridae Infections - virology ; Herpesvirus ; Japan ; Japan - epidemiology ; Prevalence ; Rivers ; Seasons ; Temperature ; Virus detection ; Virus quantification ; Wild common carp</subject><ispartof>Veterinary microbiology, 2015-02, Vol.175 (2-4), p.362-368</ispartof><rights>2014 Elsevier B.V.</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c461t-475042a3f18cb08e2f47797a6e053a0df6cf53e539e89825fae2d6ac9120b8cb3</citedby><cites>FETCH-LOGICAL-c461t-475042a3f18cb08e2f47797a6e053a0df6cf53e539e89825fae2d6ac9120b8cb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378113514005574$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25554244$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujioka, Hiroya</creatorcontrib><creatorcontrib>Yamasaki, Kenichi</creatorcontrib><creatorcontrib>Furusawa, Keiki</creatorcontrib><creatorcontrib>Tamura, Kazuki</creatorcontrib><creatorcontrib>Oguro, Kazuki</creatorcontrib><creatorcontrib>Kurihara, Sumire</creatorcontrib><creatorcontrib>Seki, Shingo</creatorcontrib><creatorcontrib>Oshima, Syun-ichirou</creatorcontrib><creatorcontrib>Imajoh, Masayuki</creatorcontrib><title>Prevalence and characteristics of Cyprinid herpesvirus 3 (CyHV-3) infection in common carp (Cyprinus carpio L.) inhabiting three rivers in Kochi Prefecture, Japan</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>•We detected and quantified CyHV-3 in 227 common carp from three rivers, Kochi, Japan.•CyHV-3 DNA was detected but infectious virus was not isolated in 23 tissue samples.•CyHV-3 DNA load did not differ statistically between sampling months.•All isolates showed similar genetic features to the past isolates in Japan.•We report a first finding of presence of circular or concatameric CyHV-3 DNA in carp.
Cyprinid herpesvirus 3 (CyHV-3) causes lethal disease in common and koi carp. Mortality by CyHV-3 disease has not been reported since 2011 in Kochi Prefecture, Japan. Here, we detected and quantified CyHV-3 in common carp inhabiting three rivers in the prefecture to examine if the carp are carriers of CyHV-3 as a source of infection. CyHV-3 DNA was detected in 16.7% (12/72) of brain samples in Kagami River, 3.9% (3/76) of brain and 3.9% (3/76) of gill samples in Monobe River, and 5.1% (4/79) of brain and 1.3% (1/79) of gill samples in Wajiki River. CyHV-3 genotypes identified in the 23 samples were classified as the J genotype A1 that has been found in Japan. The CyHV-3 DNA load did not differ statistically between sampling months, indicating that CyHV-3 has been silent in common carp, unlike Lake Biwa where the annual reactivation occurs in spring. Taken together, our results represented definitive evidence that seasonal changes in water temperature do not affect CyHV-3 activity in carp. Considering that infectious virus was not isolated from CyHV-3 DNA-positive samples, it was suggested that CyHV-3 establishes a latent infection in carp populations inhabiting Kagami River, Monobe River and Wajiki River. Further, the presence of circular or concatameric CyHV-3 DNA was detected in five of 23 CyHV-3 DNA-positive samples. Common carp inhabiting Lake Biwa were reported previously to harbor linear but not circular CyHV-3 DNA. This difference suggested that the CyHV-3 genome may be circularized for long-term maintenance without active viral replication.</description><subject>Animals</subject><subject>Carps</subject><subject>Cyprinid herpesvirus 3</subject><subject>Cyprinus carpio</subject><subject>Fish Diseases - epidemiology</subject><subject>Fish Diseases - virology</subject><subject>Genotyping</subject><subject>Herpesviridae - isolation & purification</subject><subject>Herpesviridae Infections - veterinary</subject><subject>Herpesviridae Infections - virology</subject><subject>Herpesvirus</subject><subject>Japan</subject><subject>Japan - epidemiology</subject><subject>Prevalence</subject><subject>Rivers</subject><subject>Seasons</subject><subject>Temperature</subject><subject>Virus detection</subject><subject>Virus quantification</subject><subject>Wild common carp</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi1ERZfCP0DIxyKRdPyZ5IJUrYBCV4IDcLW8zoR4tfnATiLt3-kvxdEWjoiTx9LzztjzEPKKQc6A6ZtDvuDUeZdzYDJnPAfgT8iGlYXIuJL8KdmAKMqMMaEuyfMYDwAgKw3PyCVXKhFSbsjD14CLPWLvkNq-pq61wboJg4-Td5EODd2exuB7X9MWw4hx8WGOVNDr7enuRybeUN836CY_9Kmibui6VDkbxpVYk4ler36gu3ylW7v3k-9_0qkNiDT4BUNcs_eDaz1ND1r7zQHf0s92tP0LctHYY8SXj-cV-f7h_bftXbb78vHT9naXOanZlMlCgeRWNKx0eyiRN7IoqsJqBCUs1I12jRKoRIVlVXLVWOS1tq5iHPYpIq7I9bnvGIZfM8bJdD46PB5tj8McDdMaJGhWFP-BKi6BC10lVJ5RF4YY099M2klnw8kwMKtIczBnkWYVaRg3SWSKvX6cMO87rP-G_phLwLszgGkli8dgovOrxtqHtD5TD_7fE34Du96xlQ</recordid><startdate>20150225</startdate><enddate>20150225</enddate><creator>Fujioka, Hiroya</creator><creator>Yamasaki, Kenichi</creator><creator>Furusawa, Keiki</creator><creator>Tamura, Kazuki</creator><creator>Oguro, Kazuki</creator><creator>Kurihara, Sumire</creator><creator>Seki, Shingo</creator><creator>Oshima, Syun-ichirou</creator><creator>Imajoh, Masayuki</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U9</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>20150225</creationdate><title>Prevalence and characteristics of Cyprinid herpesvirus 3 (CyHV-3) infection in common carp (Cyprinus carpio L.) inhabiting three rivers in Kochi Prefecture, Japan</title><author>Fujioka, Hiroya ; Yamasaki, Kenichi ; Furusawa, Keiki ; Tamura, Kazuki ; Oguro, Kazuki ; Kurihara, Sumire ; Seki, Shingo ; Oshima, Syun-ichirou ; Imajoh, Masayuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-475042a3f18cb08e2f47797a6e053a0df6cf53e539e89825fae2d6ac9120b8cb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Carps</topic><topic>Cyprinid herpesvirus 3</topic><topic>Cyprinus carpio</topic><topic>Fish Diseases - epidemiology</topic><topic>Fish Diseases - virology</topic><topic>Genotyping</topic><topic>Herpesviridae - isolation & purification</topic><topic>Herpesviridae Infections - veterinary</topic><topic>Herpesviridae Infections - virology</topic><topic>Herpesvirus</topic><topic>Japan</topic><topic>Japan - epidemiology</topic><topic>Prevalence</topic><topic>Rivers</topic><topic>Seasons</topic><topic>Temperature</topic><topic>Virus detection</topic><topic>Virus quantification</topic><topic>Wild common carp</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujioka, Hiroya</creatorcontrib><creatorcontrib>Yamasaki, Kenichi</creatorcontrib><creatorcontrib>Furusawa, Keiki</creatorcontrib><creatorcontrib>Tamura, Kazuki</creatorcontrib><creatorcontrib>Oguro, Kazuki</creatorcontrib><creatorcontrib>Kurihara, Sumire</creatorcontrib><creatorcontrib>Seki, Shingo</creatorcontrib><creatorcontrib>Oshima, Syun-ichirou</creatorcontrib><creatorcontrib>Imajoh, Masayuki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujioka, Hiroya</au><au>Yamasaki, Kenichi</au><au>Furusawa, Keiki</au><au>Tamura, Kazuki</au><au>Oguro, Kazuki</au><au>Kurihara, Sumire</au><au>Seki, Shingo</au><au>Oshima, Syun-ichirou</au><au>Imajoh, Masayuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence and characteristics of Cyprinid herpesvirus 3 (CyHV-3) infection in common carp (Cyprinus carpio L.) inhabiting three rivers in Kochi Prefecture, Japan</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2015-02-25</date><risdate>2015</risdate><volume>175</volume><issue>2-4</issue><spage>362</spage><epage>368</epage><pages>362-368</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><abstract>•We detected and quantified CyHV-3 in 227 common carp from three rivers, Kochi, Japan.•CyHV-3 DNA was detected but infectious virus was not isolated in 23 tissue samples.•CyHV-3 DNA load did not differ statistically between sampling months.•All isolates showed similar genetic features to the past isolates in Japan.•We report a first finding of presence of circular or concatameric CyHV-3 DNA in carp.
Cyprinid herpesvirus 3 (CyHV-3) causes lethal disease in common and koi carp. Mortality by CyHV-3 disease has not been reported since 2011 in Kochi Prefecture, Japan. Here, we detected and quantified CyHV-3 in common carp inhabiting three rivers in the prefecture to examine if the carp are carriers of CyHV-3 as a source of infection. CyHV-3 DNA was detected in 16.7% (12/72) of brain samples in Kagami River, 3.9% (3/76) of brain and 3.9% (3/76) of gill samples in Monobe River, and 5.1% (4/79) of brain and 1.3% (1/79) of gill samples in Wajiki River. CyHV-3 genotypes identified in the 23 samples were classified as the J genotype A1 that has been found in Japan. The CyHV-3 DNA load did not differ statistically between sampling months, indicating that CyHV-3 has been silent in common carp, unlike Lake Biwa where the annual reactivation occurs in spring. Taken together, our results represented definitive evidence that seasonal changes in water temperature do not affect CyHV-3 activity in carp. Considering that infectious virus was not isolated from CyHV-3 DNA-positive samples, it was suggested that CyHV-3 establishes a latent infection in carp populations inhabiting Kagami River, Monobe River and Wajiki River. Further, the presence of circular or concatameric CyHV-3 DNA was detected in five of 23 CyHV-3 DNA-positive samples. Common carp inhabiting Lake Biwa were reported previously to harbor linear but not circular CyHV-3 DNA. This difference suggested that the CyHV-3 genome may be circularized for long-term maintenance without active viral replication.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>25554244</pmid><doi>10.1016/j.vetmic.2014.12.002</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Carps Cyprinid herpesvirus 3 Cyprinus carpio Fish Diseases - epidemiology Fish Diseases - virology Genotyping Herpesviridae - isolation & purification Herpesviridae Infections - veterinary Herpesviridae Infections - virology Herpesvirus Japan Japan - epidemiology Prevalence Rivers Seasons Temperature Virus detection Virus quantification Wild common carp |
| title | Prevalence and characteristics of Cyprinid herpesvirus 3 (CyHV-3) infection in common carp (Cyprinus carpio L.) inhabiting three rivers in Kochi Prefecture, Japan |
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