Alanyl-glutamine and glutamine plus alanine supplements improve skeletal redox status in trained rats: Involvement of heat shock protein pathways

We hypothesized that oral l-glutamine supplementations could attenuate muscle damage and oxidative stress, mediated by glutathione (GSH) in high-intensity aerobic exercise by increasing the 70-kDa heat shock proteins (HSP70) and heat shock factor 1 (HSF1). Adult male Wistar rats were 8-week trained...

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Veröffentlicht in:Life sciences (1973) 2014-01, Vol.94 (2), p.130-136
Hauptverfasser: Petry, Éder Ricardo, Cruzat, Vinicius Fernandes, Heck, Thiago Gomes, Leite, Jaqueline Santos Moreira, Homem de Bittencourt, Paulo Ivo, Tirapegui, Julio
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container_end_page 136
container_issue 2
container_start_page 130
container_title Life sciences (1973)
container_volume 94
creator Petry, Éder Ricardo
Cruzat, Vinicius Fernandes
Heck, Thiago Gomes
Leite, Jaqueline Santos Moreira
Homem de Bittencourt, Paulo Ivo
Tirapegui, Julio
description We hypothesized that oral l-glutamine supplementations could attenuate muscle damage and oxidative stress, mediated by glutathione (GSH) in high-intensity aerobic exercise by increasing the 70-kDa heat shock proteins (HSP70) and heat shock factor 1 (HSF1). Adult male Wistar rats were 8-week trained (60-min/day, 5days/week) on a treadmill. During the last 21days, the animals were supplemented with either l-alanyl-l-glutamine dipeptide (1.5g/kg, DIP) or a solution containing the amino acids l-glutamine (1g/kg) and l-alanine (0.67g/kg) in their free form (GLN+ALA) or water (controls). Plasma from both DIP- and GLN+ALA-treated animals showed higher l-glutamine concentrations and reduced ammonium, malondialdehyde, myoglobin and creatine kinase activity. In the soleus and gastrocnemius muscle of both supplemented groups, l-glutamine and GSH contents were increased and GSH disulfide (GSSG) to GSH ratio was attenuated (p
doi_str_mv 10.1016/j.lfs.2013.11.009
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Adult male Wistar rats were 8-week trained (60-min/day, 5days/week) on a treadmill. During the last 21days, the animals were supplemented with either l-alanyl-l-glutamine dipeptide (1.5g/kg, DIP) or a solution containing the amino acids l-glutamine (1g/kg) and l-alanine (0.67g/kg) in their free form (GLN+ALA) or water (controls). Plasma from both DIP- and GLN+ALA-treated animals showed higher l-glutamine concentrations and reduced ammonium, malondialdehyde, myoglobin and creatine kinase activity. In the soleus and gastrocnemius muscle of both supplemented groups, l-glutamine and GSH contents were increased and GSH disulfide (GSSG) to GSH ratio was attenuated (p&lt;0.001). In the soleus muscle, cytosolic and nuclear HSP70 and HSF1 were increased by DIP supplementation. GLN+ALA group exhibited higher HSP70 (only in the nucleus) and HSF1 (cytosol and nucleus). In the gastrocnemius muscle, both supplementations were able to increase cytosolic HSP70 and cytosolic and nuclear HSF1. In trained rats, oral supplementation with DIP or GLN+ALA solution increased the expression of muscle HSP70, favored muscle l-glutamine/GSH status and improved redox defenses, which attenuate markers of muscle damage, thus improving the beneficial effects of high-intensity exercise training.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2013.11.009</identifier><identifier>PMID: 24269578</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Administration, Oral ; Alanine - administration &amp; dosage ; Alanine - pharmacology ; Animals ; Creatine Kinase - blood ; Dietary Supplements ; Dipeptides - administration &amp; dosage ; Dipeptides - pharmacology ; DNA-Binding Proteins - drug effects ; DNA-Binding Proteins - physiology ; Exercise ; Glutamine - administration &amp; dosage ; Glutamine - blood ; Glutamine - pharmacology ; Glutathione ; Glutathione - metabolism ; Heat Shock Transcription Factors ; Heat-Shock Proteins - drug effects ; Heat-Shock Proteins - physiology ; HSF1 ; HSP70 ; HSP70 Heat-Shock Proteins - drug effects ; HSP70 Heat-Shock Proteins - physiology ; Male ; Malondialdehyde - blood ; Muscle, Skeletal - drug effects ; Muscle, Skeletal - metabolism ; Muscle, Skeletal - physiology ; Myoglobin - blood ; Oxidative stress ; Oxidative Stress - drug effects ; Oxidative Stress - physiology ; Physical Conditioning, Animal - physiology ; Rats ; Rats, Wistar ; Transcription Factors - drug effects ; Transcription Factors - physiology</subject><ispartof>Life sciences (1973), 2014-01, Vol.94 (2), p.130-136</ispartof><rights>2013 Elsevier Inc.</rights><rights>Copyright © 2013 Elsevier Inc. 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Adult male Wistar rats were 8-week trained (60-min/day, 5days/week) on a treadmill. During the last 21days, the animals were supplemented with either l-alanyl-l-glutamine dipeptide (1.5g/kg, DIP) or a solution containing the amino acids l-glutamine (1g/kg) and l-alanine (0.67g/kg) in their free form (GLN+ALA) or water (controls). Plasma from both DIP- and GLN+ALA-treated animals showed higher l-glutamine concentrations and reduced ammonium, malondialdehyde, myoglobin and creatine kinase activity. In the soleus and gastrocnemius muscle of both supplemented groups, l-glutamine and GSH contents were increased and GSH disulfide (GSSG) to GSH ratio was attenuated (p&lt;0.001). In the soleus muscle, cytosolic and nuclear HSP70 and HSF1 were increased by DIP supplementation. GLN+ALA group exhibited higher HSP70 (only in the nucleus) and HSF1 (cytosol and nucleus). In the gastrocnemius muscle, both supplementations were able to increase cytosolic HSP70 and cytosolic and nuclear HSF1. In trained rats, oral supplementation with DIP or GLN+ALA solution increased the expression of muscle HSP70, favored muscle l-glutamine/GSH status and improved redox defenses, which attenuate markers of muscle damage, thus improving the beneficial effects of high-intensity exercise training.</description><subject>Administration, Oral</subject><subject>Alanine - administration &amp; dosage</subject><subject>Alanine - pharmacology</subject><subject>Animals</subject><subject>Creatine Kinase - blood</subject><subject>Dietary Supplements</subject><subject>Dipeptides - administration &amp; dosage</subject><subject>Dipeptides - pharmacology</subject><subject>DNA-Binding Proteins - drug effects</subject><subject>DNA-Binding Proteins - physiology</subject><subject>Exercise</subject><subject>Glutamine - administration &amp; dosage</subject><subject>Glutamine - blood</subject><subject>Glutamine - pharmacology</subject><subject>Glutathione</subject><subject>Glutathione - metabolism</subject><subject>Heat Shock Transcription Factors</subject><subject>Heat-Shock Proteins - drug effects</subject><subject>Heat-Shock Proteins - physiology</subject><subject>HSF1</subject><subject>HSP70</subject><subject>HSP70 Heat-Shock Proteins - drug effects</subject><subject>HSP70 Heat-Shock Proteins - physiology</subject><subject>Male</subject><subject>Malondialdehyde - blood</subject><subject>Muscle, Skeletal - drug effects</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Muscle, Skeletal - physiology</subject><subject>Myoglobin - blood</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Oxidative Stress - physiology</subject><subject>Physical Conditioning, Animal - physiology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Transcription Factors - drug effects</subject><subject>Transcription Factors - physiology</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUhi1ERYfCA7BBXrJJ8HEcJ4FVVXGpVKkbWFt2fMJ46lywnYF5DN4YD1Ng166sY33_Lx9_hLwCVgID-XZX-iGWnEFVApSMdU_IBtqmK5is4CnZMMZFUXFWn5PnMe4YY3XdVM_IORdcdnXTbsivS6-ngy---TXp0U1I9WTp_2nxa6Q6M8chrsviccQpRerGJcz7fHeHHpP2NKCdf9KYdMoJN9EUdM5YGnSK7-j1tJ_9_k-WzgPdok40buf-juaahJlfdNr-0If4gpwN2kd8eX9ekK8fP3y5-lzc3H66vrq8KXrBu1Tw2tRWDNCD6QyvNFjgLQxgwGoJvEMcpDCIVlatbhpsbQVNZZgZuG60MNUFeXPqzQ_4vmJManSxR593xXmNCqRkggloxOOo6FjDW9m2GYUT2oc5xoCDWoIbdTgoYOooTe1UlqaO0hSAytJy5vV9_WpGtP8Sfy1l4P0JwPwfe4dBxd7h1KN1Afuk7OweqP8NXPmreA</recordid><startdate>20140117</startdate><enddate>20140117</enddate><creator>Petry, Éder Ricardo</creator><creator>Cruzat, Vinicius Fernandes</creator><creator>Heck, Thiago Gomes</creator><creator>Leite, Jaqueline Santos Moreira</creator><creator>Homem de Bittencourt, Paulo Ivo</creator><creator>Tirapegui, Julio</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QP</scope></search><sort><creationdate>20140117</creationdate><title>Alanyl-glutamine and glutamine plus alanine supplements improve skeletal redox status in trained rats: Involvement of heat shock protein pathways</title><author>Petry, Éder Ricardo ; 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Adult male Wistar rats were 8-week trained (60-min/day, 5days/week) on a treadmill. During the last 21days, the animals were supplemented with either l-alanyl-l-glutamine dipeptide (1.5g/kg, DIP) or a solution containing the amino acids l-glutamine (1g/kg) and l-alanine (0.67g/kg) in their free form (GLN+ALA) or water (controls). Plasma from both DIP- and GLN+ALA-treated animals showed higher l-glutamine concentrations and reduced ammonium, malondialdehyde, myoglobin and creatine kinase activity. In the soleus and gastrocnemius muscle of both supplemented groups, l-glutamine and GSH contents were increased and GSH disulfide (GSSG) to GSH ratio was attenuated (p&lt;0.001). In the soleus muscle, cytosolic and nuclear HSP70 and HSF1 were increased by DIP supplementation. GLN+ALA group exhibited higher HSP70 (only in the nucleus) and HSF1 (cytosol and nucleus). In the gastrocnemius muscle, both supplementations were able to increase cytosolic HSP70 and cytosolic and nuclear HSF1. In trained rats, oral supplementation with DIP or GLN+ALA solution increased the expression of muscle HSP70, favored muscle l-glutamine/GSH status and improved redox defenses, which attenuate markers of muscle damage, thus improving the beneficial effects of high-intensity exercise training.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>24269578</pmid><doi>10.1016/j.lfs.2013.11.009</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
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subjects Administration, Oral
Alanine - administration & dosage
Alanine - pharmacology
Animals
Creatine Kinase - blood
Dietary Supplements
Dipeptides - administration & dosage
Dipeptides - pharmacology
DNA-Binding Proteins - drug effects
DNA-Binding Proteins - physiology
Exercise
Glutamine - administration & dosage
Glutamine - blood
Glutamine - pharmacology
Glutathione
Glutathione - metabolism
Heat Shock Transcription Factors
Heat-Shock Proteins - drug effects
Heat-Shock Proteins - physiology
HSF1
HSP70
HSP70 Heat-Shock Proteins - drug effects
HSP70 Heat-Shock Proteins - physiology
Male
Malondialdehyde - blood
Muscle, Skeletal - drug effects
Muscle, Skeletal - metabolism
Muscle, Skeletal - physiology
Myoglobin - blood
Oxidative stress
Oxidative Stress - drug effects
Oxidative Stress - physiology
Physical Conditioning, Animal - physiology
Rats
Rats, Wistar
Transcription Factors - drug effects
Transcription Factors - physiology
title Alanyl-glutamine and glutamine plus alanine supplements improve skeletal redox status in trained rats: Involvement of heat shock protein pathways
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