Alanyl-glutamine and glutamine plus alanine supplements improve skeletal redox status in trained rats: Involvement of heat shock protein pathways
We hypothesized that oral l-glutamine supplementations could attenuate muscle damage and oxidative stress, mediated by glutathione (GSH) in high-intensity aerobic exercise by increasing the 70-kDa heat shock proteins (HSP70) and heat shock factor 1 (HSF1). Adult male Wistar rats were 8-week trained...
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creator | Petry, Éder Ricardo Cruzat, Vinicius Fernandes Heck, Thiago Gomes Leite, Jaqueline Santos Moreira Homem de Bittencourt, Paulo Ivo Tirapegui, Julio |
description | We hypothesized that oral l-glutamine supplementations could attenuate muscle damage and oxidative stress, mediated by glutathione (GSH) in high-intensity aerobic exercise by increasing the 70-kDa heat shock proteins (HSP70) and heat shock factor 1 (HSF1).
Adult male Wistar rats were 8-week trained (60-min/day, 5days/week) on a treadmill. During the last 21days, the animals were supplemented with either l-alanyl-l-glutamine dipeptide (1.5g/kg, DIP) or a solution containing the amino acids l-glutamine (1g/kg) and l-alanine (0.67g/kg) in their free form (GLN+ALA) or water (controls).
Plasma from both DIP- and GLN+ALA-treated animals showed higher l-glutamine concentrations and reduced ammonium, malondialdehyde, myoglobin and creatine kinase activity. In the soleus and gastrocnemius muscle of both supplemented groups, l-glutamine and GSH contents were increased and GSH disulfide (GSSG) to GSH ratio was attenuated (p |
doi_str_mv | 10.1016/j.lfs.2013.11.009 |
format | Article |
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Adult male Wistar rats were 8-week trained (60-min/day, 5days/week) on a treadmill. During the last 21days, the animals were supplemented with either l-alanyl-l-glutamine dipeptide (1.5g/kg, DIP) or a solution containing the amino acids l-glutamine (1g/kg) and l-alanine (0.67g/kg) in their free form (GLN+ALA) or water (controls).
Plasma from both DIP- and GLN+ALA-treated animals showed higher l-glutamine concentrations and reduced ammonium, malondialdehyde, myoglobin and creatine kinase activity. In the soleus and gastrocnemius muscle of both supplemented groups, l-glutamine and GSH contents were increased and GSH disulfide (GSSG) to GSH ratio was attenuated (p<0.001). In the soleus muscle, cytosolic and nuclear HSP70 and HSF1 were increased by DIP supplementation. GLN+ALA group exhibited higher HSP70 (only in the nucleus) and HSF1 (cytosol and nucleus). In the gastrocnemius muscle, both supplementations were able to increase cytosolic HSP70 and cytosolic and nuclear HSF1.
In trained rats, oral supplementation with DIP or GLN+ALA solution increased the expression of muscle HSP70, favored muscle l-glutamine/GSH status and improved redox defenses, which attenuate markers of muscle damage, thus improving the beneficial effects of high-intensity exercise training.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2013.11.009</identifier><identifier>PMID: 24269578</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Administration, Oral ; Alanine - administration & dosage ; Alanine - pharmacology ; Animals ; Creatine Kinase - blood ; Dietary Supplements ; Dipeptides - administration & dosage ; Dipeptides - pharmacology ; DNA-Binding Proteins - drug effects ; DNA-Binding Proteins - physiology ; Exercise ; Glutamine - administration & dosage ; Glutamine - blood ; Glutamine - pharmacology ; Glutathione ; Glutathione - metabolism ; Heat Shock Transcription Factors ; Heat-Shock Proteins - drug effects ; Heat-Shock Proteins - physiology ; HSF1 ; HSP70 ; HSP70 Heat-Shock Proteins - drug effects ; HSP70 Heat-Shock Proteins - physiology ; Male ; Malondialdehyde - blood ; Muscle, Skeletal - drug effects ; Muscle, Skeletal - metabolism ; Muscle, Skeletal - physiology ; Myoglobin - blood ; Oxidative stress ; Oxidative Stress - drug effects ; Oxidative Stress - physiology ; Physical Conditioning, Animal - physiology ; Rats ; Rats, Wistar ; Transcription Factors - drug effects ; Transcription Factors - physiology</subject><ispartof>Life sciences (1973), 2014-01, Vol.94 (2), p.130-136</ispartof><rights>2013 Elsevier Inc.</rights><rights>Copyright © 2013 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-25b5d4f1c1b9b23a1d1281f1b1da6129eef64beed638a77e8d3173b0bf2a7a4b3</citedby><cites>FETCH-LOGICAL-c429t-25b5d4f1c1b9b23a1d1281f1b1da6129eef64beed638a77e8d3173b0bf2a7a4b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.lfs.2013.11.009$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24269578$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Petry, Éder Ricardo</creatorcontrib><creatorcontrib>Cruzat, Vinicius Fernandes</creatorcontrib><creatorcontrib>Heck, Thiago Gomes</creatorcontrib><creatorcontrib>Leite, Jaqueline Santos Moreira</creatorcontrib><creatorcontrib>Homem de Bittencourt, Paulo Ivo</creatorcontrib><creatorcontrib>Tirapegui, Julio</creatorcontrib><title>Alanyl-glutamine and glutamine plus alanine supplements improve skeletal redox status in trained rats: Involvement of heat shock protein pathways</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>We hypothesized that oral l-glutamine supplementations could attenuate muscle damage and oxidative stress, mediated by glutathione (GSH) in high-intensity aerobic exercise by increasing the 70-kDa heat shock proteins (HSP70) and heat shock factor 1 (HSF1).
Adult male Wistar rats were 8-week trained (60-min/day, 5days/week) on a treadmill. During the last 21days, the animals were supplemented with either l-alanyl-l-glutamine dipeptide (1.5g/kg, DIP) or a solution containing the amino acids l-glutamine (1g/kg) and l-alanine (0.67g/kg) in their free form (GLN+ALA) or water (controls).
Plasma from both DIP- and GLN+ALA-treated animals showed higher l-glutamine concentrations and reduced ammonium, malondialdehyde, myoglobin and creatine kinase activity. In the soleus and gastrocnemius muscle of both supplemented groups, l-glutamine and GSH contents were increased and GSH disulfide (GSSG) to GSH ratio was attenuated (p<0.001). In the soleus muscle, cytosolic and nuclear HSP70 and HSF1 were increased by DIP supplementation. GLN+ALA group exhibited higher HSP70 (only in the nucleus) and HSF1 (cytosol and nucleus). In the gastrocnemius muscle, both supplementations were able to increase cytosolic HSP70 and cytosolic and nuclear HSF1.
In trained rats, oral supplementation with DIP or GLN+ALA solution increased the expression of muscle HSP70, favored muscle l-glutamine/GSH status and improved redox defenses, which attenuate markers of muscle damage, thus improving the beneficial effects of high-intensity exercise training.</description><subject>Administration, Oral</subject><subject>Alanine - administration & dosage</subject><subject>Alanine - pharmacology</subject><subject>Animals</subject><subject>Creatine Kinase - blood</subject><subject>Dietary Supplements</subject><subject>Dipeptides - administration & dosage</subject><subject>Dipeptides - pharmacology</subject><subject>DNA-Binding Proteins - drug effects</subject><subject>DNA-Binding Proteins - physiology</subject><subject>Exercise</subject><subject>Glutamine - administration & dosage</subject><subject>Glutamine - blood</subject><subject>Glutamine - pharmacology</subject><subject>Glutathione</subject><subject>Glutathione - metabolism</subject><subject>Heat Shock Transcription Factors</subject><subject>Heat-Shock Proteins - drug effects</subject><subject>Heat-Shock Proteins - physiology</subject><subject>HSF1</subject><subject>HSP70</subject><subject>HSP70 Heat-Shock Proteins - drug effects</subject><subject>HSP70 Heat-Shock Proteins - physiology</subject><subject>Male</subject><subject>Malondialdehyde - blood</subject><subject>Muscle, Skeletal - drug effects</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Muscle, Skeletal - physiology</subject><subject>Myoglobin - blood</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Oxidative Stress - physiology</subject><subject>Physical Conditioning, Animal - physiology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Transcription Factors - drug effects</subject><subject>Transcription Factors - physiology</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUhi1ERYfCA7BBXrJJ8HEcJ4FVVXGpVKkbWFt2fMJ46lywnYF5DN4YD1Ng166sY33_Lx9_hLwCVgID-XZX-iGWnEFVApSMdU_IBtqmK5is4CnZMMZFUXFWn5PnMe4YY3XdVM_IORdcdnXTbsivS6-ngy---TXp0U1I9WTp_2nxa6Q6M8chrsviccQpRerGJcz7fHeHHpP2NKCdf9KYdMoJN9EUdM5YGnSK7-j1tJ_9_k-WzgPdok40buf-juaahJlfdNr-0If4gpwN2kd8eX9ekK8fP3y5-lzc3H66vrq8KXrBu1Tw2tRWDNCD6QyvNFjgLQxgwGoJvEMcpDCIVlatbhpsbQVNZZgZuG60MNUFeXPqzQ_4vmJManSxR593xXmNCqRkggloxOOo6FjDW9m2GYUT2oc5xoCDWoIbdTgoYOooTe1UlqaO0hSAytJy5vV9_WpGtP8Sfy1l4P0JwPwfe4dBxd7h1KN1Afuk7OweqP8NXPmreA</recordid><startdate>20140117</startdate><enddate>20140117</enddate><creator>Petry, Éder Ricardo</creator><creator>Cruzat, Vinicius Fernandes</creator><creator>Heck, Thiago Gomes</creator><creator>Leite, Jaqueline Santos Moreira</creator><creator>Homem de Bittencourt, Paulo Ivo</creator><creator>Tirapegui, Julio</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QP</scope></search><sort><creationdate>20140117</creationdate><title>Alanyl-glutamine and glutamine plus alanine supplements improve skeletal redox status in trained rats: Involvement of heat shock protein pathways</title><author>Petry, Éder Ricardo ; Cruzat, Vinicius Fernandes ; Heck, Thiago Gomes ; Leite, Jaqueline Santos Moreira ; Homem de Bittencourt, Paulo Ivo ; Tirapegui, Julio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-25b5d4f1c1b9b23a1d1281f1b1da6129eef64beed638a77e8d3173b0bf2a7a4b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Administration, Oral</topic><topic>Alanine - administration & dosage</topic><topic>Alanine - pharmacology</topic><topic>Animals</topic><topic>Creatine Kinase - blood</topic><topic>Dietary Supplements</topic><topic>Dipeptides - administration & dosage</topic><topic>Dipeptides - pharmacology</topic><topic>DNA-Binding Proteins - drug effects</topic><topic>DNA-Binding Proteins - physiology</topic><topic>Exercise</topic><topic>Glutamine - administration & dosage</topic><topic>Glutamine - blood</topic><topic>Glutamine - pharmacology</topic><topic>Glutathione</topic><topic>Glutathione - metabolism</topic><topic>Heat Shock Transcription Factors</topic><topic>Heat-Shock Proteins - drug effects</topic><topic>Heat-Shock Proteins - physiology</topic><topic>HSF1</topic><topic>HSP70</topic><topic>HSP70 Heat-Shock Proteins - drug effects</topic><topic>HSP70 Heat-Shock Proteins - physiology</topic><topic>Male</topic><topic>Malondialdehyde - blood</topic><topic>Muscle, Skeletal - drug effects</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Muscle, Skeletal - physiology</topic><topic>Myoglobin - blood</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Oxidative Stress - physiology</topic><topic>Physical Conditioning, Animal - physiology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Transcription Factors - drug effects</topic><topic>Transcription Factors - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Petry, Éder Ricardo</creatorcontrib><creatorcontrib>Cruzat, Vinicius Fernandes</creatorcontrib><creatorcontrib>Heck, Thiago Gomes</creatorcontrib><creatorcontrib>Leite, Jaqueline Santos Moreira</creatorcontrib><creatorcontrib>Homem de Bittencourt, Paulo Ivo</creatorcontrib><creatorcontrib>Tirapegui, Julio</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Calcium & Calcified Tissue Abstracts</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Petry, Éder Ricardo</au><au>Cruzat, Vinicius Fernandes</au><au>Heck, Thiago Gomes</au><au>Leite, Jaqueline Santos Moreira</au><au>Homem de Bittencourt, Paulo Ivo</au><au>Tirapegui, Julio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alanyl-glutamine and glutamine plus alanine supplements improve skeletal redox status in trained rats: Involvement of heat shock protein pathways</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2014-01-17</date><risdate>2014</risdate><volume>94</volume><issue>2</issue><spage>130</spage><epage>136</epage><pages>130-136</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>We hypothesized that oral l-glutamine supplementations could attenuate muscle damage and oxidative stress, mediated by glutathione (GSH) in high-intensity aerobic exercise by increasing the 70-kDa heat shock proteins (HSP70) and heat shock factor 1 (HSF1).
Adult male Wistar rats were 8-week trained (60-min/day, 5days/week) on a treadmill. During the last 21days, the animals were supplemented with either l-alanyl-l-glutamine dipeptide (1.5g/kg, DIP) or a solution containing the amino acids l-glutamine (1g/kg) and l-alanine (0.67g/kg) in their free form (GLN+ALA) or water (controls).
Plasma from both DIP- and GLN+ALA-treated animals showed higher l-glutamine concentrations and reduced ammonium, malondialdehyde, myoglobin and creatine kinase activity. In the soleus and gastrocnemius muscle of both supplemented groups, l-glutamine and GSH contents were increased and GSH disulfide (GSSG) to GSH ratio was attenuated (p<0.001). In the soleus muscle, cytosolic and nuclear HSP70 and HSF1 were increased by DIP supplementation. GLN+ALA group exhibited higher HSP70 (only in the nucleus) and HSF1 (cytosol and nucleus). In the gastrocnemius muscle, both supplementations were able to increase cytosolic HSP70 and cytosolic and nuclear HSF1.
In trained rats, oral supplementation with DIP or GLN+ALA solution increased the expression of muscle HSP70, favored muscle l-glutamine/GSH status and improved redox defenses, which attenuate markers of muscle damage, thus improving the beneficial effects of high-intensity exercise training.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>24269578</pmid><doi>10.1016/j.lfs.2013.11.009</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
subjects | Administration, Oral Alanine - administration & dosage Alanine - pharmacology Animals Creatine Kinase - blood Dietary Supplements Dipeptides - administration & dosage Dipeptides - pharmacology DNA-Binding Proteins - drug effects DNA-Binding Proteins - physiology Exercise Glutamine - administration & dosage Glutamine - blood Glutamine - pharmacology Glutathione Glutathione - metabolism Heat Shock Transcription Factors Heat-Shock Proteins - drug effects Heat-Shock Proteins - physiology HSF1 HSP70 HSP70 Heat-Shock Proteins - drug effects HSP70 Heat-Shock Proteins - physiology Male Malondialdehyde - blood Muscle, Skeletal - drug effects Muscle, Skeletal - metabolism Muscle, Skeletal - physiology Myoglobin - blood Oxidative stress Oxidative Stress - drug effects Oxidative Stress - physiology Physical Conditioning, Animal - physiology Rats Rats, Wistar Transcription Factors - drug effects Transcription Factors - physiology |
title | Alanyl-glutamine and glutamine plus alanine supplements improve skeletal redox status in trained rats: Involvement of heat shock protein pathways |
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