Function of a novel C-type lectin with two CRD domains from Macrobrachium rosenbergii in innate immunity
•A novel C-type lectin with two CRD domains was identified from Macrobrachium rosenbergii.•MrCTL was regulated by Vibrio parahaemolyticus and WSSV.•rMrCTL, rMrCRD1 and rMrCRD2 could bind different bacteria.•rMrCTL, rMrCRD1 and rMrCRD2 could agglutinate bacteria in a Ca2+-dependent manner. C-type lec...
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| Veröffentlicht in: | Developmental and comparative immunology 2015-03, Vol.49 (1), p.121-126 |
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| creator | Huang, Xin Huang, Ying Shi, Yan-Ru Ren, Qian Wang, Wen |
| description | •A novel C-type lectin with two CRD domains was identified from Macrobrachium rosenbergii.•MrCTL was regulated by Vibrio parahaemolyticus and WSSV.•rMrCTL, rMrCRD1 and rMrCRD2 could bind different bacteria.•rMrCTL, rMrCRD1 and rMrCRD2 could agglutinate bacteria in a Ca2+-dependent manner.
C-type lectins play crucial roles in innate immunity. In the present study, a novel C-type lectin gene, designated as MrCTL, was identified from Macrobrachium rosenbergii. MrCTL contains 2 carbohydrate-recognition domains (CRDs), namely MrCRD1 and MrCRD2. The MrCRD1 contains a QEP motif and MrCRD2 contains a motif of EPD. MrCTL was mainly expressed in the hepatopancreas. The expression level of MrCTL in hepatopancreas was significantly upregulated after a challenge with Vibrio parahaemolyticus or White spot syndrome virus (WSSV). The recombinant MrCTL, MrCRD1 and MrCRD2 have an ability to agglutinate both Gram-negative (V. parahaemolyticus) and Gram-positive bacteria (Staphylococcus aureus) in a calcium dependent manner. The recombinant MrCTL, MrCRD1 and MrCRD2 bind directly to all tested microorganisms. All these results suggested that MrCTL may have important roles in immune defense against invading pathogens in prawns. |
| doi_str_mv | 10.1016/j.dci.2014.11.015 |
| format | Article |
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C-type lectins play crucial roles in innate immunity. In the present study, a novel C-type lectin gene, designated as MrCTL, was identified from Macrobrachium rosenbergii. MrCTL contains 2 carbohydrate-recognition domains (CRDs), namely MrCRD1 and MrCRD2. The MrCRD1 contains a QEP motif and MrCRD2 contains a motif of EPD. MrCTL was mainly expressed in the hepatopancreas. The expression level of MrCTL in hepatopancreas was significantly upregulated after a challenge with Vibrio parahaemolyticus or White spot syndrome virus (WSSV). The recombinant MrCTL, MrCRD1 and MrCRD2 have an ability to agglutinate both Gram-negative (V. parahaemolyticus) and Gram-positive bacteria (Staphylococcus aureus) in a calcium dependent manner. The recombinant MrCTL, MrCRD1 and MrCRD2 bind directly to all tested microorganisms. All these results suggested that MrCTL may have important roles in immune defense against invading pathogens in prawns.</description><identifier>ISSN: 0145-305X</identifier><identifier>EISSN: 1879-0089</identifier><identifier>DOI: 10.1016/j.dci.2014.11.015</identifier><identifier>PMID: 25475962</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Arthropod Proteins - classification ; Arthropod Proteins - genetics ; Arthropod Proteins - immunology ; Base Sequence ; Binding Sites - immunology ; C-type lectin ; Gene Expression Profiling ; Host-Pathogen Interactions - immunology ; Immunity, Innate - immunology ; Innate immunity ; Lectins, C-Type - classification ; Lectins, C-Type - genetics ; Lectins, C-Type - immunology ; Macrobrachium rosenbergii ; Molecular Sequence Data ; Palaemonidae - immunology ; Palaemonidae - microbiology ; Palaemonidae - virology ; Pattern recognition receptor ; Phylogeny ; Recombinant Proteins - immunology ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Amino Acid ; Staphylococcus aureus ; Staphylococcus aureus - immunology ; Vibrio parahaemolyticus ; Vibrio parahaemolyticus - immunology ; Vibrio parahaemolyticus - physiology ; White spot syndrome virus ; White spot syndrome virus 1 - immunology ; White spot syndrome virus 1 - physiology</subject><ispartof>Developmental and comparative immunology, 2015-03, Vol.49 (1), p.121-126</ispartof><rights>2014 Elsevier Ltd</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-5adbc5ffa309d301360ba314afa0a9b70e70545455471126bc3eaef3df99af573</citedby><cites>FETCH-LOGICAL-c386t-5adbc5ffa309d301360ba314afa0a9b70e70545455471126bc3eaef3df99af573</cites><orcidid>0000-0002-2487-471X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.dci.2014.11.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25475962$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Xin</creatorcontrib><creatorcontrib>Huang, Ying</creatorcontrib><creatorcontrib>Shi, Yan-Ru</creatorcontrib><creatorcontrib>Ren, Qian</creatorcontrib><creatorcontrib>Wang, Wen</creatorcontrib><title>Function of a novel C-type lectin with two CRD domains from Macrobrachium rosenbergii in innate immunity</title><title>Developmental and comparative immunology</title><addtitle>Dev Comp Immunol</addtitle><description>•A novel C-type lectin with two CRD domains was identified from Macrobrachium rosenbergii.•MrCTL was regulated by Vibrio parahaemolyticus and WSSV.•rMrCTL, rMrCRD1 and rMrCRD2 could bind different bacteria.•rMrCTL, rMrCRD1 and rMrCRD2 could agglutinate bacteria in a Ca2+-dependent manner.
C-type lectins play crucial roles in innate immunity. In the present study, a novel C-type lectin gene, designated as MrCTL, was identified from Macrobrachium rosenbergii. MrCTL contains 2 carbohydrate-recognition domains (CRDs), namely MrCRD1 and MrCRD2. The MrCRD1 contains a QEP motif and MrCRD2 contains a motif of EPD. MrCTL was mainly expressed in the hepatopancreas. The expression level of MrCTL in hepatopancreas was significantly upregulated after a challenge with Vibrio parahaemolyticus or White spot syndrome virus (WSSV). The recombinant MrCTL, MrCRD1 and MrCRD2 have an ability to agglutinate both Gram-negative (V. parahaemolyticus) and Gram-positive bacteria (Staphylococcus aureus) in a calcium dependent manner. The recombinant MrCTL, MrCRD1 and MrCRD2 bind directly to all tested microorganisms. All these results suggested that MrCTL may have important roles in immune defense against invading pathogens in prawns.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Arthropod Proteins - classification</subject><subject>Arthropod Proteins - genetics</subject><subject>Arthropod Proteins - immunology</subject><subject>Base Sequence</subject><subject>Binding Sites - immunology</subject><subject>C-type lectin</subject><subject>Gene Expression Profiling</subject><subject>Host-Pathogen Interactions - immunology</subject><subject>Immunity, Innate - immunology</subject><subject>Innate immunity</subject><subject>Lectins, C-Type - classification</subject><subject>Lectins, C-Type - genetics</subject><subject>Lectins, C-Type - immunology</subject><subject>Macrobrachium rosenbergii</subject><subject>Molecular Sequence Data</subject><subject>Palaemonidae - immunology</subject><subject>Palaemonidae - microbiology</subject><subject>Palaemonidae - virology</subject><subject>Pattern recognition receptor</subject><subject>Phylogeny</subject><subject>Recombinant Proteins - immunology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Sequence Homology, Amino Acid</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - immunology</subject><subject>Vibrio parahaemolyticus</subject><subject>Vibrio parahaemolyticus - immunology</subject><subject>Vibrio parahaemolyticus - physiology</subject><subject>White spot syndrome virus</subject><subject>White spot syndrome virus 1 - immunology</subject><subject>White spot syndrome virus 1 - physiology</subject><issn>0145-305X</issn><issn>1879-0089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EokvhA3BBPnJJmInj_BEntKWA1AqpKhI3y3HGrFeJvdhJq_32uNrCkXoOPszvPWneY-wtQomAzYd9ORpXVoB1iVgCymdsg13bFwBd_5xt8kIWAuTPM_YqpT3k1yG8ZGeVrFvZN9WG7S5XbxYXPA-Wa-7DHU18WyzHA_GJ8sbze7fs-HIf-Pbmgo9h1s4nbmOY-bU2MQxRm51bZx5DIj9Q_OUczzLnvV6Iu3levVuOr9kLq6dEbx7_c_bj8vPt9mtx9f3Lt-2nq8KIrlkKqcfBSGu1gH4UgKKBQQustdWg-6EFakHWefIFiFUzGEGarBht32srW3HO3p98DzH8XiktanbJ0DRpT2FNCpsGahBdnqfRDFZtjSKjeELzwSlFsuoQ3azjUSGohy7UXuUu1EMXClHlLrLm3aP9Osw0_lP8DT8DH08A5TzuHEWVjCNvaHQxR6_G4P5j_wcUg5nV</recordid><startdate>201503</startdate><enddate>201503</enddate><creator>Huang, Xin</creator><creator>Huang, Ying</creator><creator>Shi, Yan-Ru</creator><creator>Ren, Qian</creator><creator>Wang, Wen</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>L.G</scope><orcidid>https://orcid.org/0000-0002-2487-471X</orcidid></search><sort><creationdate>201503</creationdate><title>Function of a novel C-type lectin with two CRD domains from Macrobrachium rosenbergii in innate immunity</title><author>Huang, Xin ; Huang, Ying ; Shi, Yan-Ru ; Ren, Qian ; Wang, Wen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-5adbc5ffa309d301360ba314afa0a9b70e70545455471126bc3eaef3df99af573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Arthropod Proteins - classification</topic><topic>Arthropod Proteins - genetics</topic><topic>Arthropod Proteins - immunology</topic><topic>Base Sequence</topic><topic>Binding Sites - immunology</topic><topic>C-type lectin</topic><topic>Gene Expression Profiling</topic><topic>Host-Pathogen Interactions - immunology</topic><topic>Immunity, Innate - immunology</topic><topic>Innate immunity</topic><topic>Lectins, C-Type - classification</topic><topic>Lectins, C-Type - genetics</topic><topic>Lectins, C-Type - immunology</topic><topic>Macrobrachium rosenbergii</topic><topic>Molecular Sequence Data</topic><topic>Palaemonidae - immunology</topic><topic>Palaemonidae - microbiology</topic><topic>Palaemonidae - virology</topic><topic>Pattern recognition receptor</topic><topic>Phylogeny</topic><topic>Recombinant Proteins - immunology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Sequence Homology, Amino Acid</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - immunology</topic><topic>Vibrio parahaemolyticus</topic><topic>Vibrio parahaemolyticus - immunology</topic><topic>Vibrio parahaemolyticus - physiology</topic><topic>White spot syndrome virus</topic><topic>White spot syndrome virus 1 - immunology</topic><topic>White spot syndrome virus 1 - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Xin</creatorcontrib><creatorcontrib>Huang, Ying</creatorcontrib><creatorcontrib>Shi, Yan-Ru</creatorcontrib><creatorcontrib>Ren, Qian</creatorcontrib><creatorcontrib>Wang, Wen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Developmental and comparative immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Xin</au><au>Huang, Ying</au><au>Shi, Yan-Ru</au><au>Ren, Qian</au><au>Wang, Wen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Function of a novel C-type lectin with two CRD domains from Macrobrachium rosenbergii in innate immunity</atitle><jtitle>Developmental and comparative immunology</jtitle><addtitle>Dev Comp Immunol</addtitle><date>2015-03</date><risdate>2015</risdate><volume>49</volume><issue>1</issue><spage>121</spage><epage>126</epage><pages>121-126</pages><issn>0145-305X</issn><eissn>1879-0089</eissn><abstract>•A novel C-type lectin with two CRD domains was identified from Macrobrachium rosenbergii.•MrCTL was regulated by Vibrio parahaemolyticus and WSSV.•rMrCTL, rMrCRD1 and rMrCRD2 could bind different bacteria.•rMrCTL, rMrCRD1 and rMrCRD2 could agglutinate bacteria in a Ca2+-dependent manner.
C-type lectins play crucial roles in innate immunity. In the present study, a novel C-type lectin gene, designated as MrCTL, was identified from Macrobrachium rosenbergii. MrCTL contains 2 carbohydrate-recognition domains (CRDs), namely MrCRD1 and MrCRD2. The MrCRD1 contains a QEP motif and MrCRD2 contains a motif of EPD. MrCTL was mainly expressed in the hepatopancreas. The expression level of MrCTL in hepatopancreas was significantly upregulated after a challenge with Vibrio parahaemolyticus or White spot syndrome virus (WSSV). The recombinant MrCTL, MrCRD1 and MrCRD2 have an ability to agglutinate both Gram-negative (V. parahaemolyticus) and Gram-positive bacteria (Staphylococcus aureus) in a calcium dependent manner. The recombinant MrCTL, MrCRD1 and MrCRD2 bind directly to all tested microorganisms. All these results suggested that MrCTL may have important roles in immune defense against invading pathogens in prawns.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>25475962</pmid><doi>10.1016/j.dci.2014.11.015</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-2487-471X</orcidid></addata></record> |
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| subjects | Amino Acid Sequence Animals Arthropod Proteins - classification Arthropod Proteins - genetics Arthropod Proteins - immunology Base Sequence Binding Sites - immunology C-type lectin Gene Expression Profiling Host-Pathogen Interactions - immunology Immunity, Innate - immunology Innate immunity Lectins, C-Type - classification Lectins, C-Type - genetics Lectins, C-Type - immunology Macrobrachium rosenbergii Molecular Sequence Data Palaemonidae - immunology Palaemonidae - microbiology Palaemonidae - virology Pattern recognition receptor Phylogeny Recombinant Proteins - immunology Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Staphylococcus aureus Staphylococcus aureus - immunology Vibrio parahaemolyticus Vibrio parahaemolyticus - immunology Vibrio parahaemolyticus - physiology White spot syndrome virus White spot syndrome virus 1 - immunology White spot syndrome virus 1 - physiology |
| title | Function of a novel C-type lectin with two CRD domains from Macrobrachium rosenbergii in innate immunity |
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