Molecular cloning of a laccase gene from Ganoderma lucidum and heterologous expression in Pichia pastoris

A genomic laccase gene and cDNA were cloned from the white‐rot fungi Ganoderma lucidum TR6. The genomic laccase gene contained 2086 bp with nine introns. The laccase cDNA had an open reading frame of 1563 bp. The deduced mature protein consisted of 520 amino acids. Both the genomic laccase gene and...

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Veröffentlicht in:	Journal of basic microbiology 2014-07, Vol.54 (S1), p.S134-S141
Hauptverfasser:	You, Lin-Feng, Liu, Zhi-Ming, Lin, Jun-Fang, Guo, Li-Qiong, Huang, Xun-Liu, Yang, Hai-Xing
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Complementary - genetics DNA, Fungal - chemistry DNA, Fungal - genetics Enzyme Stability Ganoderma lucidum Gene Expression Gene Expression Profiling Gene Expression Regulation, Fungal - drug effects Gene Expression Regulation, Fungal - radiation effects Genetic Vectors Heterologous expression Hydrogen-Ion Concentration Introns Kinetics Laccase Laccase - chemistry Laccase - genetics Laccase - isolation & purification Laccase - metabolism Molecular Sequence Data Molecular Weight Nitrogen - metabolism Open Reading Frames Pichia - enzymology Pichia - genetics Pichia pastoris Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Reishi - enzymology Reishi - genetics Sequence Analysis, DNA Temperature
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