Laser capture microdissection after γ-glutamyl transferase histochemistry: An optimization for gene expression analysis

γ-Glutamyl transferase (GGT) is useful as a marker in pathological conditions, including several types of cancer. We optimized the histochemical detection of GGT to assay the gene expression profiles of phenotype-specific cells selected by laser capture microdissection (LCM). For optimization, we us...

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Veröffentlicht in:	Analytical biochemistry 2014-02, Vol.447, p.126-132
Hauptverfasser:	Torres Mena, Julia Esperanza, Sánchez Rodríguez, Ricardo, Quintanar Jurado, Valeria, Mojica Espinosa, Raúl, Del Pozo Yauner, Luis, Meléndez Zajgla, Jorge, Villa Treviño, Saúl, Pérez Carreón, Julio Isael
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Atherosclerosis Carcinoma DNA microarrays gamma-Glutamyltransferase - genetics gamma-Glutamyltransferase - metabolism Gene Expression Profiling - methods Immunohistochemistry - methods Laser Capture Microdissection - methods Liver - cytology Liver neoplasms Oligonucleotide Array Sequence Analysis Rats Reverse transcriptase PCR RNA RNA, Messenger - genetics RNA, Messenger - metabolism
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