Identification and characterization of a novel copper transporter gene family TaCT1 in common wheat
Copper is an essential micronutrient for plant growth and development, and copper transporter plays a pivotal role for keeping copper homeostasis. However, little is known about copper transporters in wheat. Here, we report a novel copper transporter gene family, TaCT1, in common wheat. Three TaCT1...
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| Veröffentlicht in: | Plant, cell and environment cell and environment, 2014-07, Vol.37 (7), p.1561-1573 |
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| creator | LI, HAOXUN FAN, RENCHUN LI, LIBIN WEI, BO LI, GUOLIANG GU, LIQING WANG, XIANPING ZHANG, XIANGQI |
| description | Copper is an essential micronutrient for plant growth and development, and copper transporter plays a pivotal role for keeping copper homeostasis. However, little is known about copper transporters in wheat. Here, we report a novel copper transporter gene family, TaCT1, in common wheat. Three TaCT1 homoeologous genes were isolated and assigned to group 5 chromosomes. Each of the TaCT1 genes (TaCT1‐5A, −5B or −5D) possesses 12 transmembrane domains. TaCT1 genes exhibited higher transcript levels in leaf than in root, culm and spikelet. Excess copper down‐regulated the transcript levels of TaCT1 and copper deficiency‐induced TaCT1 expression. Subcellular experiments localized the TaCT1 to the Golgi apparatus. Yeast expression experiments and virus‐induced gene silencing analysis indicated that the TaCT1 functioned in copper transport. Site‐directed mutagenesis demonstrated that three amino acid residues, Met³⁵, Met³⁸ and Cys³⁶⁵, are required for TaCT1 function. Phylogenetic and functional analyses suggested that homologous genes shared high similarity with TaCT1 may exist exclusively in monocot plants. Our work reveals a novel wheat gene family encoding major facilitator superfamily (MFS)‐type copper transporters, and provides evidence for their functional involvement in promoting copper uptake and keeping copper homeostasis in common wheat. |
| doi_str_mv | 10.1111/pce.12263 |
| format | Article |
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However, little is known about copper transporters in wheat. Here, we report a novel copper transporter gene family, TaCT1, in common wheat. Three TaCT1 homoeologous genes were isolated and assigned to group 5 chromosomes. Each of the TaCT1 genes (TaCT1‐5A, −5B or −5D) possesses 12 transmembrane domains. TaCT1 genes exhibited higher transcript levels in leaf than in root, culm and spikelet. Excess copper down‐regulated the transcript levels of TaCT1 and copper deficiency‐induced TaCT1 expression. Subcellular experiments localized the TaCT1 to the Golgi apparatus. Yeast expression experiments and virus‐induced gene silencing analysis indicated that the TaCT1 functioned in copper transport. Site‐directed mutagenesis demonstrated that three amino acid residues, Met³⁵, Met³⁸ and Cys³⁶⁵, are required for TaCT1 function. Phylogenetic and functional analyses suggested that homologous genes shared high similarity with TaCT1 may exist exclusively in monocot plants. Our work reveals a novel wheat gene family encoding major facilitator superfamily (MFS)‐type copper transporters, and provides evidence for their functional involvement in promoting copper uptake and keeping copper homeostasis in common wheat.</description><identifier>ISSN: 0140-7791</identifier><identifier>EISSN: 1365-3040</identifier><identifier>DOI: 10.1111/pce.12263</identifier><identifier>PMID: 24372025</identifier><identifier>CODEN: PLCEDV</identifier><language>eng</language><publisher>Oxford: Blackwell Science</publisher><subject>Amino Acids - metabolism ; Base Sequence ; Biological and medical sciences ; Biological Transport - drug effects ; chromosomes ; Chromosomes, Plant - genetics ; copper ; Copper - metabolism ; Copper - toxicity ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Plant - drug effects ; Gene Knockdown Techniques ; gene silencing ; genes ; Genes, Plant ; Genetic Complementation Test ; Golgi apparatus ; growth and development ; homeostasis ; leaves ; Membrane Transport Proteins - genetics ; Membrane Transport Proteins - metabolism ; Molecular Sequence Data ; Multigene Family ; mutagenesis ; Organ Specificity - drug effects ; Organ Specificity - genetics ; phylogenetic analysis ; Phylogeny ; plant growth ; plant micronutrients ; Plant Proteins - genetics ; Plant Proteins - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Saccharomyces cerevisiae - metabolism ; site‐directed mutagenesis ; Stress, Physiological - drug effects ; Stress, Physiological - genetics ; Subcellular Fractions - metabolism ; transporters ; Triticum - drug effects ; Triticum - genetics ; Triticum - metabolism ; Triticum aestivum ; virus‐induced gene silencing ; wheat ; yeasts</subject><ispartof>Plant, cell and environment, 2014-07, Vol.37 (7), p.1561-1573</ispartof><rights>2013 John Wiley & Sons Ltd</rights><rights>2015 INIST-CNRS</rights><rights>2013 John Wiley & Sons Ltd.</rights><rights>Copyright © 2014 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fpce.12263$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fpce.12263$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28516784$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24372025$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LI, HAOXUN</creatorcontrib><creatorcontrib>FAN, RENCHUN</creatorcontrib><creatorcontrib>LI, LIBIN</creatorcontrib><creatorcontrib>WEI, BO</creatorcontrib><creatorcontrib>LI, GUOLIANG</creatorcontrib><creatorcontrib>GU, LIQING</creatorcontrib><creatorcontrib>WANG, XIANPING</creatorcontrib><creatorcontrib>ZHANG, XIANGQI</creatorcontrib><title>Identification and characterization of a novel copper transporter gene family TaCT1 in common wheat</title><title>Plant, cell and environment</title><addtitle>Plant Cell Environ</addtitle><description>Copper is an essential micronutrient for plant growth and development, and copper transporter plays a pivotal role for keeping copper homeostasis. However, little is known about copper transporters in wheat. Here, we report a novel copper transporter gene family, TaCT1, in common wheat. Three TaCT1 homoeologous genes were isolated and assigned to group 5 chromosomes. Each of the TaCT1 genes (TaCT1‐5A, −5B or −5D) possesses 12 transmembrane domains. TaCT1 genes exhibited higher transcript levels in leaf than in root, culm and spikelet. Excess copper down‐regulated the transcript levels of TaCT1 and copper deficiency‐induced TaCT1 expression. Subcellular experiments localized the TaCT1 to the Golgi apparatus. Yeast expression experiments and virus‐induced gene silencing analysis indicated that the TaCT1 functioned in copper transport. Site‐directed mutagenesis demonstrated that three amino acid residues, Met³⁵, Met³⁸ and Cys³⁶⁵, are required for TaCT1 function. Phylogenetic and functional analyses suggested that homologous genes shared high similarity with TaCT1 may exist exclusively in monocot plants. Our work reveals a novel wheat gene family encoding major facilitator superfamily (MFS)‐type copper transporters, and provides evidence for their functional involvement in promoting copper uptake and keeping copper homeostasis in common wheat.</description><subject>Amino Acids - metabolism</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biological Transport - drug effects</subject><subject>chromosomes</subject><subject>Chromosomes, Plant - genetics</subject><subject>copper</subject><subject>Copper - metabolism</subject><subject>Copper - toxicity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Gene Knockdown Techniques</subject><subject>gene silencing</subject><subject>genes</subject><subject>Genes, Plant</subject><subject>Genetic Complementation Test</subject><subject>Golgi apparatus</subject><subject>growth and development</subject><subject>homeostasis</subject><subject>leaves</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>mutagenesis</subject><subject>Organ Specificity - drug effects</subject><subject>Organ Specificity - genetics</subject><subject>phylogenetic analysis</subject><subject>Phylogeny</subject><subject>plant growth</subject><subject>plant micronutrients</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>site‐directed mutagenesis</subject><subject>Stress, Physiological - drug effects</subject><subject>Stress, Physiological - genetics</subject><subject>Subcellular Fractions - metabolism</subject><subject>transporters</subject><subject>Triticum - drug effects</subject><subject>Triticum - genetics</subject><subject>Triticum - metabolism</subject><subject>Triticum aestivum</subject><subject>virus‐induced gene silencing</subject><subject>wheat</subject><subject>yeasts</subject><issn>0140-7791</issn><issn>1365-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkVFrHCEUhaU0NJu0D_0DrVACfZnk6jjqPJYlaQOBFLp5Fte5JoaZcaqzDdtfHze7baG-KNfvHOUcQt4zOGdlXUwOzxnnsn5FFqyWTVWDgNdkAUxApVTLjslJzo8AZaDaN-SYi1px4M2CuOsOxzn44Owc4kjt2FH3YJN1M6bwez-Mnlo6xl_YUxenCROdkx3zFFOB6D2OSL0dQr-lK7tcMRrGwg1DUT49oJ3fkiNv-4zvDvspubu6XC2_VTe3X6-XX24qL4DVlWrWAtyadY3WVndeCceU104wqRxw0UqvJEgExTgTXHXee5Raoxe41k1bn5LPe98pxZ8bzLMZQnbY93bEuMmGSVlyAVXv0E__oY9xk8byO8OaGtryJNeF-nCgNusBOzOlMNi0NX_iK8DZAbDZ2d6XVFzI_zjdFCMtCnex555Cj9u_9wzMrj9T-jMv_Znvy8uXQ1F83Cu8jcbep-J694PvCgUG0BTiGfKOlBY</recordid><startdate>201407</startdate><enddate>201407</enddate><creator>LI, HAOXUN</creator><creator>FAN, RENCHUN</creator><creator>LI, LIBIN</creator><creator>WEI, BO</creator><creator>LI, GUOLIANG</creator><creator>GU, LIQING</creator><creator>WANG, XIANPING</creator><creator>ZHANG, XIANGQI</creator><general>Blackwell Science</general><general>Blackwell</general><general>Wiley Subscription Services, Inc</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QP</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201407</creationdate><title>Identification and characterization of a novel copper transporter gene family TaCT1 in common wheat</title><author>LI, HAOXUN ; FAN, RENCHUN ; LI, LIBIN ; WEI, BO ; LI, GUOLIANG ; GU, LIQING ; WANG, XIANPING ; ZHANG, XIANGQI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f4013-75b40cb1d588a8df74c17f8c4167c02496f7606e07121427dfffe688ef4eb8593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Amino Acids - metabolism</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biological Transport - drug effects</topic><topic>chromosomes</topic><topic>Chromosomes, Plant - genetics</topic><topic>copper</topic><topic>Copper - metabolism</topic><topic>Copper - toxicity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Gene Knockdown Techniques</topic><topic>gene silencing</topic><topic>genes</topic><topic>Genes, Plant</topic><topic>Genetic Complementation Test</topic><topic>Golgi apparatus</topic><topic>growth and development</topic><topic>homeostasis</topic><topic>leaves</topic><topic>Membrane Transport Proteins - genetics</topic><topic>Membrane Transport Proteins - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>mutagenesis</topic><topic>Organ Specificity - drug effects</topic><topic>Organ Specificity - genetics</topic><topic>phylogenetic analysis</topic><topic>Phylogeny</topic><topic>plant growth</topic><topic>plant micronutrients</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>site‐directed mutagenesis</topic><topic>Stress, Physiological - drug effects</topic><topic>Stress, Physiological - genetics</topic><topic>Subcellular Fractions - metabolism</topic><topic>transporters</topic><topic>Triticum - drug effects</topic><topic>Triticum - genetics</topic><topic>Triticum - metabolism</topic><topic>Triticum aestivum</topic><topic>virus‐induced gene silencing</topic><topic>wheat</topic><topic>yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LI, HAOXUN</creatorcontrib><creatorcontrib>FAN, RENCHUN</creatorcontrib><creatorcontrib>LI, LIBIN</creatorcontrib><creatorcontrib>WEI, BO</creatorcontrib><creatorcontrib>LI, GUOLIANG</creatorcontrib><creatorcontrib>GU, LIQING</creatorcontrib><creatorcontrib>WANG, XIANPING</creatorcontrib><creatorcontrib>ZHANG, XIANGQI</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant, cell and environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LI, HAOXUN</au><au>FAN, RENCHUN</au><au>LI, LIBIN</au><au>WEI, BO</au><au>LI, GUOLIANG</au><au>GU, LIQING</au><au>WANG, XIANPING</au><au>ZHANG, XIANGQI</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of a novel copper transporter gene family TaCT1 in common wheat</atitle><jtitle>Plant, cell and environment</jtitle><addtitle>Plant Cell Environ</addtitle><date>2014-07</date><risdate>2014</risdate><volume>37</volume><issue>7</issue><spage>1561</spage><epage>1573</epage><pages>1561-1573</pages><issn>0140-7791</issn><eissn>1365-3040</eissn><coden>PLCEDV</coden><abstract>Copper is an essential micronutrient for plant growth and development, and copper transporter plays a pivotal role for keeping copper homeostasis. However, little is known about copper transporters in wheat. Here, we report a novel copper transporter gene family, TaCT1, in common wheat. Three TaCT1 homoeologous genes were isolated and assigned to group 5 chromosomes. Each of the TaCT1 genes (TaCT1‐5A, −5B or −5D) possesses 12 transmembrane domains. TaCT1 genes exhibited higher transcript levels in leaf than in root, culm and spikelet. Excess copper down‐regulated the transcript levels of TaCT1 and copper deficiency‐induced TaCT1 expression. Subcellular experiments localized the TaCT1 to the Golgi apparatus. Yeast expression experiments and virus‐induced gene silencing analysis indicated that the TaCT1 functioned in copper transport. Site‐directed mutagenesis demonstrated that three amino acid residues, Met³⁵, Met³⁸ and Cys³⁶⁵, are required for TaCT1 function. Phylogenetic and functional analyses suggested that homologous genes shared high similarity with TaCT1 may exist exclusively in monocot plants. Our work reveals a novel wheat gene family encoding major facilitator superfamily (MFS)‐type copper transporters, and provides evidence for their functional involvement in promoting copper uptake and keeping copper homeostasis in common wheat.</abstract><cop>Oxford</cop><pub>Blackwell Science</pub><pmid>24372025</pmid><doi>10.1111/pce.12263</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acids - metabolism Base Sequence Biological and medical sciences Biological Transport - drug effects chromosomes Chromosomes, Plant - genetics copper Copper - metabolism Copper - toxicity Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant - drug effects Gene Knockdown Techniques gene silencing genes Genes, Plant Genetic Complementation Test Golgi apparatus growth and development homeostasis leaves Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Molecular Sequence Data Multigene Family mutagenesis Organ Specificity - drug effects Organ Specificity - genetics phylogenetic analysis Phylogeny plant growth plant micronutrients Plant Proteins - genetics Plant Proteins - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Saccharomyces cerevisiae - metabolism site‐directed mutagenesis Stress, Physiological - drug effects Stress, Physiological - genetics Subcellular Fractions - metabolism transporters Triticum - drug effects Triticum - genetics Triticum - metabolism Triticum aestivum virus‐induced gene silencing wheat yeasts |
| title | Identification and characterization of a novel copper transporter gene family TaCT1 in common wheat |
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