Bacterial Pathogen Gene Abundance and Relation to Recreational Water Quality at Seven Great Lakes Beaches
Quantitative assessment of bacterial pathogens, their geographic variability, and distribution in various matrices at Great Lakes beaches are limited. Quantitative PCR (qPCR) was used to test for genes from E. coli O157:H7 (eae O157), shiga-toxin producing E. coli (stx2), Campylobacter jejuni (mapA)...
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| Veröffentlicht in: | Environmental science & technology 2014-12, Vol.48 (24), p.14148-14157 |
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| creator | Oster, Ryan J Wijesinghe, Rasanthi U Haack, Sheridan K Fogarty, Lisa R Tucker, Taaja R Riley, Stephen C |
| description | Quantitative assessment of bacterial pathogens, their geographic variability, and distribution in various matrices at Great Lakes beaches are limited. Quantitative PCR (qPCR) was used to test for genes from E. coli O157:H7 (eae O157), shiga-toxin producing E. coli (stx2), Campylobacter jejuni (mapA), Shigella spp. (ipaH), and a Salmonella enterica-specific (SE) DNA sequence at seven Great Lakes beaches, in algae, water, and sediment. Overall, detection frequencies were mapA>stx2>ipaH>SE>eae O157 . Results were highly variable among beaches and matrices; some correlations with environmental conditions were observed for mapA, stx2, and ipaH detections. Beach seasonal mean mapA abundance in water was correlated with beach seasonal mean log10 E. coli concentration. At one beach, stx2 gene abundance was positively correlated with concurrent daily E. coli concentrations. Concentration distributions for stx2, ipaH, and mapA within algae, sediment, and water were statistically different (Non-Detect and Data Analysis in R). Assuming 10, 50, or 100% of gene copies represented viable and presumably infective cells, a quantitative microbial risk assessment tool developed by Michigan State University indicated a moderate probability of illness for Campylobacter jejuni at the study beaches, especially where recreational water quality criteria were exceeded. Pathogen gene quantification may be useful for beach water quality management. |
| doi_str_mv | 10.1021/es5038657 |
| format | Article |
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Quantitative PCR (qPCR) was used to test for genes from E. coli O157:H7 (eae O157), shiga-toxin producing E. coli (stx2), Campylobacter jejuni (mapA), Shigella spp. (ipaH), and a Salmonella enterica-specific (SE) DNA sequence at seven Great Lakes beaches, in algae, water, and sediment. Overall, detection frequencies were mapA>stx2>ipaH>SE>eae O157 . Results were highly variable among beaches and matrices; some correlations with environmental conditions were observed for mapA, stx2, and ipaH detections. Beach seasonal mean mapA abundance in water was correlated with beach seasonal mean log10 E. coli concentration. At one beach, stx2 gene abundance was positively correlated with concurrent daily E. coli concentrations. Concentration distributions for stx2, ipaH, and mapA within algae, sediment, and water were statistically different (Non-Detect and Data Analysis in R). Assuming 10, 50, or 100% of gene copies represented viable and presumably infective cells, a quantitative microbial risk assessment tool developed by Michigan State University indicated a moderate probability of illness for Campylobacter jejuni at the study beaches, especially where recreational water quality criteria were exceeded. Pathogen gene quantification may be useful for beach water quality management.</description><identifier>ISSN: 0013-936X</identifier><identifier>EISSN: 1520-5851</identifier><identifier>DOI: 10.1021/es5038657</identifier><identifier>PMID: 25423586</identifier><identifier>CODEN: ESTHAG</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animal, plant and microbial ecology ; Applied sciences ; Bacteria ; Bacteria - genetics ; Bacteria - isolation & purification ; Bacterial Proteins - genetics ; Bathing Beaches ; Beaches ; Biological and medical sciences ; Campylobacter ; Campylobacter jejuni ; E coli ; Environmental Monitoring ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; Genes ; Great Lakes Region ; Lakes - microbiology ; Microbial ecology ; Natural water pollution ; Pathogens ; Pollution ; Polymerase Chain Reaction ; Salmonella ; Seawaters, estuaries ; Various environments (extraatmospheric space, air, water) ; Water Quality ; Water treatment and pollution</subject><ispartof>Environmental science & technology, 2014-12, Vol.48 (24), p.14148-14157</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright American Chemical Society Dec 16, 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a472t-8ad5af12bdcc513ff5710c34631273577fa635f2280193b0702d879e2c5540bd3</citedby><cites>FETCH-LOGICAL-a472t-8ad5af12bdcc513ff5710c34631273577fa635f2280193b0702d879e2c5540bd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/es5038657$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/es5038657$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27055,27903,27904,56716,56766</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=29086265$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25423586$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oster, Ryan J</creatorcontrib><creatorcontrib>Wijesinghe, Rasanthi U</creatorcontrib><creatorcontrib>Haack, Sheridan K</creatorcontrib><creatorcontrib>Fogarty, Lisa R</creatorcontrib><creatorcontrib>Tucker, Taaja R</creatorcontrib><creatorcontrib>Riley, Stephen C</creatorcontrib><title>Bacterial Pathogen Gene Abundance and Relation to Recreational Water Quality at Seven Great Lakes Beaches</title><title>Environmental science & technology</title><addtitle>Environ. Sci. Technol</addtitle><description>Quantitative assessment of bacterial pathogens, their geographic variability, and distribution in various matrices at Great Lakes beaches are limited. Quantitative PCR (qPCR) was used to test for genes from E. coli O157:H7 (eae O157), shiga-toxin producing E. coli (stx2), Campylobacter jejuni (mapA), Shigella spp. (ipaH), and a Salmonella enterica-specific (SE) DNA sequence at seven Great Lakes beaches, in algae, water, and sediment. Overall, detection frequencies were mapA>stx2>ipaH>SE>eae O157 . Results were highly variable among beaches and matrices; some correlations with environmental conditions were observed for mapA, stx2, and ipaH detections. Beach seasonal mean mapA abundance in water was correlated with beach seasonal mean log10 E. coli concentration. At one beach, stx2 gene abundance was positively correlated with concurrent daily E. coli concentrations. Concentration distributions for stx2, ipaH, and mapA within algae, sediment, and water were statistically different (Non-Detect and Data Analysis in R). Assuming 10, 50, or 100% of gene copies represented viable and presumably infective cells, a quantitative microbial risk assessment tool developed by Michigan State University indicated a moderate probability of illness for Campylobacter jejuni at the study beaches, especially where recreational water quality criteria were exceeded. Pathogen gene quantification may be useful for beach water quality management.</description><subject>Animal, plant and microbial ecology</subject><subject>Applied sciences</subject><subject>Bacteria</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Bacterial Proteins - genetics</subject><subject>Bathing Beaches</subject><subject>Beaches</subject><subject>Biological and medical sciences</subject><subject>Campylobacter</subject><subject>Campylobacter jejuni</subject><subject>E coli</subject><subject>Environmental Monitoring</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Great Lakes Region</subject><subject>Lakes - microbiology</subject><subject>Microbial ecology</subject><subject>Natural water pollution</subject><subject>Pathogens</subject><subject>Pollution</subject><subject>Polymerase Chain Reaction</subject><subject>Salmonella</subject><subject>Seawaters, estuaries</subject><subject>Various environments (extraatmospheric space, air, water)</subject><subject>Water Quality</subject><subject>Water treatment and pollution</subject><issn>0013-936X</issn><issn>1520-5851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0U1P3DAQBmALtYIt5dA_UFmqKrWHtGM7_joCKrTSSv1EcIsmzqQEsgm1k0r8e7ywhYpeerItPTMezcvYCwHvBEjxnpIG5Yy2W2whtIRCOy2esAWAUIVX5myHPUvpAgCkArfNdqQupdLOLFh3gGGi2GHPv-B0Pv6kgR_TQHy_nocGh0Ach4Z_ox6nbhz4NOZ7iHT7ykWnmKv51xn7brrmOPHv9HvdYi34Ei8p8QPCcE7pOXvaYp9ob3PuspOjDz8OPxbLz8efDveXBZZWToXDRmMrZN2EoIVqW20FBFUaJaRV2toWjdKtlA6EVzVYkI2znmTQuoS6UbvszV3fqzj-milN1apLgfoeBxrnVAljQPm8LfkfVFltvPZlpq8e0YtxjnkDt8o57511Wb29UyGOKUVqq6vYrTBeVwKqdVTVfVTZvtx0nOsVNffyTzYZvN4ATAH7NuY0uvTgPDgjjX5wGNJfU_3z4Q1bE6Qo</recordid><startdate>20141216</startdate><enddate>20141216</enddate><creator>Oster, Ryan J</creator><creator>Wijesinghe, Rasanthi U</creator><creator>Haack, Sheridan K</creator><creator>Fogarty, Lisa R</creator><creator>Tucker, Taaja R</creator><creator>Riley, Stephen C</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><scope>7QH</scope><scope>7QL</scope><scope>7TG</scope><scope>7TV</scope><scope>7U1</scope><scope>7U2</scope><scope>7UA</scope><scope>KL.</scope></search><sort><creationdate>20141216</creationdate><title>Bacterial Pathogen Gene Abundance and Relation to Recreational Water Quality at Seven Great Lakes Beaches</title><author>Oster, Ryan J ; Wijesinghe, Rasanthi U ; Haack, Sheridan K ; Fogarty, Lisa R ; Tucker, Taaja R ; Riley, Stephen C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a472t-8ad5af12bdcc513ff5710c34631273577fa635f2280193b0702d879e2c5540bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animal, plant and microbial ecology</topic><topic>Applied sciences</topic><topic>Bacteria</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation & purification</topic><topic>Bacterial Proteins - genetics</topic><topic>Bathing Beaches</topic><topic>Beaches</topic><topic>Biological and medical sciences</topic><topic>Campylobacter</topic><topic>Campylobacter jejuni</topic><topic>E coli</topic><topic>Environmental Monitoring</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Great Lakes Region</topic><topic>Lakes - microbiology</topic><topic>Microbial ecology</topic><topic>Natural water pollution</topic><topic>Pathogens</topic><topic>Pollution</topic><topic>Polymerase Chain Reaction</topic><topic>Salmonella</topic><topic>Seawaters, estuaries</topic><topic>Various environments (extraatmospheric space, air, water)</topic><topic>Water Quality</topic><topic>Water treatment and pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oster, Ryan J</creatorcontrib><creatorcontrib>Wijesinghe, Rasanthi U</creatorcontrib><creatorcontrib>Haack, Sheridan K</creatorcontrib><creatorcontrib>Fogarty, Lisa R</creatorcontrib><creatorcontrib>Tucker, Taaja R</creatorcontrib><creatorcontrib>Riley, Stephen C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Pollution Abstracts</collection><collection>Risk Abstracts</collection><collection>Safety Science and Risk</collection><collection>Water Resources Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><jtitle>Environmental science & technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oster, Ryan J</au><au>Wijesinghe, Rasanthi U</au><au>Haack, Sheridan K</au><au>Fogarty, Lisa R</au><au>Tucker, Taaja R</au><au>Riley, Stephen C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacterial Pathogen Gene Abundance and Relation to Recreational Water Quality at Seven Great Lakes Beaches</atitle><jtitle>Environmental science & technology</jtitle><addtitle>Environ. Sci. Technol</addtitle><date>2014-12-16</date><risdate>2014</risdate><volume>48</volume><issue>24</issue><spage>14148</spage><epage>14157</epage><pages>14148-14157</pages><issn>0013-936X</issn><eissn>1520-5851</eissn><coden>ESTHAG</coden><abstract>Quantitative assessment of bacterial pathogens, their geographic variability, and distribution in various matrices at Great Lakes beaches are limited. Quantitative PCR (qPCR) was used to test for genes from E. coli O157:H7 (eae O157), shiga-toxin producing E. coli (stx2), Campylobacter jejuni (mapA), Shigella spp. (ipaH), and a Salmonella enterica-specific (SE) DNA sequence at seven Great Lakes beaches, in algae, water, and sediment. Overall, detection frequencies were mapA>stx2>ipaH>SE>eae O157 . Results were highly variable among beaches and matrices; some correlations with environmental conditions were observed for mapA, stx2, and ipaH detections. Beach seasonal mean mapA abundance in water was correlated with beach seasonal mean log10 E. coli concentration. At one beach, stx2 gene abundance was positively correlated with concurrent daily E. coli concentrations. Concentration distributions for stx2, ipaH, and mapA within algae, sediment, and water were statistically different (Non-Detect and Data Analysis in R). Assuming 10, 50, or 100% of gene copies represented viable and presumably infective cells, a quantitative microbial risk assessment tool developed by Michigan State University indicated a moderate probability of illness for Campylobacter jejuni at the study beaches, especially where recreational water quality criteria were exceeded. Pathogen gene quantification may be useful for beach water quality management.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>25423586</pmid><doi>10.1021/es5038657</doi><tpages>10</tpages></addata></record> |
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| subjects | Animal, plant and microbial ecology Applied sciences Bacteria Bacteria - genetics Bacteria - isolation & purification Bacterial Proteins - genetics Bathing Beaches Beaches Biological and medical sciences Campylobacter Campylobacter jejuni E coli Environmental Monitoring Exact sciences and technology Fundamental and applied biological sciences. Psychology Genes Great Lakes Region Lakes - microbiology Microbial ecology Natural water pollution Pathogens Pollution Polymerase Chain Reaction Salmonella Seawaters, estuaries Various environments (extraatmospheric space, air, water) Water Quality Water treatment and pollution |
| title | Bacterial Pathogen Gene Abundance and Relation to Recreational Water Quality at Seven Great Lakes Beaches |
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