Digital gene expression analysis in hemocytes of the white shrimp Litopenaeus vannamei in response to low salinity stress
The white shrimp Litopenaeus vannamei has been greatly impacted by low salinity stress. To gain knowledge on the immune response in L. vannamei under such stress, we investigated digital gene expression (DEG) in L. vannamei hemocytes using the deep-sequencing platform Illumina HiSeq 2000. In total,...
Gespeichert in:
| Veröffentlicht in: | Fish & shellfish immunology 2015-02, Vol.42 (2), p.400-407 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 407 |
|---|---|
| container_issue | 2 |
| container_start_page | 400 |
| container_title | Fish & shellfish immunology |
| container_volume | 42 |
| creator | Zhao, Qun Pan, Luqing Ren, Qin Hu, Dongxu |
| description | The white shrimp Litopenaeus vannamei has been greatly impacted by low salinity stress. To gain knowledge on the immune response in L. vannamei under such stress, we investigated digital gene expression (DEG) in L. vannamei hemocytes using the deep-sequencing platform Illumina HiSeq 2000. In total, 38,155 high quality unigenes with average length 770 bp were generated; 145 and 79 genes were identified up- or down-regulated, respectively. Functional categorization and pathways of the differentially expressed genes revealed that immune signaling pathways, cellular immunity, humoral immunity, apoptosis, cellular protein synthesis, lipid transport and energy metabolism were the differentially regulated processes occurring during low salinity stress. These results will provide a resource for subsequent gene expression studies regarding environmental stress and a valuable gene information for a better understanding of immune mechanisms of L. vannamei under low salinity stress.
•DGE profiling in hemocytes of Litopenaeus vannamei under low salinity stress.•224 high quality genes were differentially expressed under low salinity stress.•Genes were categorized into 1560 GO terms and 81 KEGG pathways.•Some important differentially expressed genes were verified via QPCR.•For a better understanding of immune mechanism of L. vannamei in salinity stress. |
| doi_str_mv | 10.1016/j.fsi.2014.11.020 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1660398527</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S105046481400446X</els_id><sourcerecordid>1660398527</sourcerecordid><originalsourceid>FETCH-LOGICAL-c386t-c01a32d9c06c70fa68cd8d41aa797164aa6f552caaa2444f22f0e3c5ba0ee5c93</originalsourceid><addsrcrecordid>eNqNkU1v1DAQhiNERUvLD-CCfOSSMHYcJxEn1PIlrcSlPVtTZ9L1KrFDxtuSf0-iLRwRp5nD874jzZNlbyUUEqT5cCh69oUCqQspC1DwIruQ0FZ52-r65bZXkGujm_PsNfMBAExp4FV2riptStWqi2y58Q8-4SAeKJCgX9NMzD4GgQGHhT0LH8SexuiWRCxiL9KexNPeJxK8n_04iZ1PcaKAdGTxiCHgSH5LrU1TDEwiRTHEJ8E4-ODTIjhtR66ysx4HpjfP8zK7-_L59vpbvvvx9fv1p13uysak3IHEUnWtA-Nq6NE0rms6LRHrtpZGI5q-qpRDRKW17pXqgUpX3SMQVa4tL7P3p95pjj-PxMmOnh0NAwaKR7bSGCjbplL1f6BaGdloaVZUnlA3R-aZejutz8B5sRLsJsce7CrHbnKslHaVs2bePdcf70fq_ib-2FiBjyeA1n88epotO0_BUedncsl20f-j_jevk6H3</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1642618416</pqid></control><display><type>article</type><title>Digital gene expression analysis in hemocytes of the white shrimp Litopenaeus vannamei in response to low salinity stress</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Zhao, Qun ; Pan, Luqing ; Ren, Qin ; Hu, Dongxu</creator><creatorcontrib>Zhao, Qun ; Pan, Luqing ; Ren, Qin ; Hu, Dongxu</creatorcontrib><description>The white shrimp Litopenaeus vannamei has been greatly impacted by low salinity stress. To gain knowledge on the immune response in L. vannamei under such stress, we investigated digital gene expression (DEG) in L. vannamei hemocytes using the deep-sequencing platform Illumina HiSeq 2000. In total, 38,155 high quality unigenes with average length 770 bp were generated; 145 and 79 genes were identified up- or down-regulated, respectively. Functional categorization and pathways of the differentially expressed genes revealed that immune signaling pathways, cellular immunity, humoral immunity, apoptosis, cellular protein synthesis, lipid transport and energy metabolism were the differentially regulated processes occurring during low salinity stress. These results will provide a resource for subsequent gene expression studies regarding environmental stress and a valuable gene information for a better understanding of immune mechanisms of L. vannamei under low salinity stress.
•DGE profiling in hemocytes of Litopenaeus vannamei under low salinity stress.•224 high quality genes were differentially expressed under low salinity stress.•Genes were categorized into 1560 GO terms and 81 KEGG pathways.•Some important differentially expressed genes were verified via QPCR.•For a better understanding of immune mechanism of L. vannamei in salinity stress.</description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2014.11.020</identifier><identifier>PMID: 25463292</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Arthropod Proteins - genetics ; Arthropod Proteins - metabolism ; Decapoda ; Digital gene expression ; Gene Library ; Hemocyte ; Hemocytes - immunology ; Hemocytes - metabolism ; High-Throughput Nucleotide Sequencing ; Immunity, Innate ; Litopenaeus vannamei ; Low salinity stress ; Penaeidae - genetics ; Penaeidae - immunology ; Penaeidae - physiology ; Random Allocation ; Salinity ; Stress, Physiological ; Transcriptome</subject><ispartof>Fish & shellfish immunology, 2015-02, Vol.42 (2), p.400-407</ispartof><rights>2014 Elsevier Ltd</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-c01a32d9c06c70fa68cd8d41aa797164aa6f552caaa2444f22f0e3c5ba0ee5c93</citedby><cites>FETCH-LOGICAL-c386t-c01a32d9c06c70fa68cd8d41aa797164aa6f552caaa2444f22f0e3c5ba0ee5c93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fsi.2014.11.020$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25463292$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Qun</creatorcontrib><creatorcontrib>Pan, Luqing</creatorcontrib><creatorcontrib>Ren, Qin</creatorcontrib><creatorcontrib>Hu, Dongxu</creatorcontrib><title>Digital gene expression analysis in hemocytes of the white shrimp Litopenaeus vannamei in response to low salinity stress</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>The white shrimp Litopenaeus vannamei has been greatly impacted by low salinity stress. To gain knowledge on the immune response in L. vannamei under such stress, we investigated digital gene expression (DEG) in L. vannamei hemocytes using the deep-sequencing platform Illumina HiSeq 2000. In total, 38,155 high quality unigenes with average length 770 bp were generated; 145 and 79 genes were identified up- or down-regulated, respectively. Functional categorization and pathways of the differentially expressed genes revealed that immune signaling pathways, cellular immunity, humoral immunity, apoptosis, cellular protein synthesis, lipid transport and energy metabolism were the differentially regulated processes occurring during low salinity stress. These results will provide a resource for subsequent gene expression studies regarding environmental stress and a valuable gene information for a better understanding of immune mechanisms of L. vannamei under low salinity stress.
•DGE profiling in hemocytes of Litopenaeus vannamei under low salinity stress.•224 high quality genes were differentially expressed under low salinity stress.•Genes were categorized into 1560 GO terms and 81 KEGG pathways.•Some important differentially expressed genes were verified via QPCR.•For a better understanding of immune mechanism of L. vannamei in salinity stress.</description><subject>Animals</subject><subject>Arthropod Proteins - genetics</subject><subject>Arthropod Proteins - metabolism</subject><subject>Decapoda</subject><subject>Digital gene expression</subject><subject>Gene Library</subject><subject>Hemocyte</subject><subject>Hemocytes - immunology</subject><subject>Hemocytes - metabolism</subject><subject>High-Throughput Nucleotide Sequencing</subject><subject>Immunity, Innate</subject><subject>Litopenaeus vannamei</subject><subject>Low salinity stress</subject><subject>Penaeidae - genetics</subject><subject>Penaeidae - immunology</subject><subject>Penaeidae - physiology</subject><subject>Random Allocation</subject><subject>Salinity</subject><subject>Stress, Physiological</subject><subject>Transcriptome</subject><issn>1050-4648</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhiNERUvLD-CCfOSSMHYcJxEn1PIlrcSlPVtTZ9L1KrFDxtuSf0-iLRwRp5nD874jzZNlbyUUEqT5cCh69oUCqQspC1DwIruQ0FZ52-r65bZXkGujm_PsNfMBAExp4FV2riptStWqi2y58Q8-4SAeKJCgX9NMzD4GgQGHhT0LH8SexuiWRCxiL9KexNPeJxK8n_04iZ1PcaKAdGTxiCHgSH5LrU1TDEwiRTHEJ8E4-ODTIjhtR66ysx4HpjfP8zK7-_L59vpbvvvx9fv1p13uysak3IHEUnWtA-Nq6NE0rms6LRHrtpZGI5q-qpRDRKW17pXqgUpX3SMQVa4tL7P3p95pjj-PxMmOnh0NAwaKR7bSGCjbplL1f6BaGdloaVZUnlA3R-aZejutz8B5sRLsJsce7CrHbnKslHaVs2bePdcf70fq_ib-2FiBjyeA1n88epotO0_BUedncsl20f-j_jevk6H3</recordid><startdate>201502</startdate><enddate>201502</enddate><creator>Zhao, Qun</creator><creator>Pan, Luqing</creator><creator>Ren, Qin</creator><creator>Hu, Dongxu</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>7TN</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201502</creationdate><title>Digital gene expression analysis in hemocytes of the white shrimp Litopenaeus vannamei in response to low salinity stress</title><author>Zhao, Qun ; Pan, Luqing ; Ren, Qin ; Hu, Dongxu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-c01a32d9c06c70fa68cd8d41aa797164aa6f552caaa2444f22f0e3c5ba0ee5c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Arthropod Proteins - genetics</topic><topic>Arthropod Proteins - metabolism</topic><topic>Decapoda</topic><topic>Digital gene expression</topic><topic>Gene Library</topic><topic>Hemocyte</topic><topic>Hemocytes - immunology</topic><topic>Hemocytes - metabolism</topic><topic>High-Throughput Nucleotide Sequencing</topic><topic>Immunity, Innate</topic><topic>Litopenaeus vannamei</topic><topic>Low salinity stress</topic><topic>Penaeidae - genetics</topic><topic>Penaeidae - immunology</topic><topic>Penaeidae - physiology</topic><topic>Random Allocation</topic><topic>Salinity</topic><topic>Stress, Physiological</topic><topic>Transcriptome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Qun</creatorcontrib><creatorcontrib>Pan, Luqing</creatorcontrib><creatorcontrib>Ren, Qin</creatorcontrib><creatorcontrib>Hu, Dongxu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Qun</au><au>Pan, Luqing</au><au>Ren, Qin</au><au>Hu, Dongxu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Digital gene expression analysis in hemocytes of the white shrimp Litopenaeus vannamei in response to low salinity stress</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2015-02</date><risdate>2015</risdate><volume>42</volume><issue>2</issue><spage>400</spage><epage>407</epage><pages>400-407</pages><issn>1050-4648</issn><eissn>1095-9947</eissn><abstract>The white shrimp Litopenaeus vannamei has been greatly impacted by low salinity stress. To gain knowledge on the immune response in L. vannamei under such stress, we investigated digital gene expression (DEG) in L. vannamei hemocytes using the deep-sequencing platform Illumina HiSeq 2000. In total, 38,155 high quality unigenes with average length 770 bp were generated; 145 and 79 genes were identified up- or down-regulated, respectively. Functional categorization and pathways of the differentially expressed genes revealed that immune signaling pathways, cellular immunity, humoral immunity, apoptosis, cellular protein synthesis, lipid transport and energy metabolism were the differentially regulated processes occurring during low salinity stress. These results will provide a resource for subsequent gene expression studies regarding environmental stress and a valuable gene information for a better understanding of immune mechanisms of L. vannamei under low salinity stress.
•DGE profiling in hemocytes of Litopenaeus vannamei under low salinity stress.•224 high quality genes were differentially expressed under low salinity stress.•Genes were categorized into 1560 GO terms and 81 KEGG pathways.•Some important differentially expressed genes were verified via QPCR.•For a better understanding of immune mechanism of L. vannamei in salinity stress.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>25463292</pmid><doi>10.1016/j.fsi.2014.11.020</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1050-4648 |
| ispartof | Fish & shellfish immunology, 2015-02, Vol.42 (2), p.400-407 |
| issn | 1050-4648 1095-9947 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1660398527 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Arthropod Proteins - genetics Arthropod Proteins - metabolism Decapoda Digital gene expression Gene Library Hemocyte Hemocytes - immunology Hemocytes - metabolism High-Throughput Nucleotide Sequencing Immunity, Innate Litopenaeus vannamei Low salinity stress Penaeidae - genetics Penaeidae - immunology Penaeidae - physiology Random Allocation Salinity Stress, Physiological Transcriptome |
| title | Digital gene expression analysis in hemocytes of the white shrimp Litopenaeus vannamei in response to low salinity stress |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T05%3A38%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Digital%20gene%20expression%20analysis%20in%20hemocytes%20of%20the%20white%20shrimp%20Litopenaeus%20vannamei%20in%20response%20to%20low%20salinity%20stress&rft.jtitle=Fish%20&%20shellfish%20immunology&rft.au=Zhao,%20Qun&rft.date=2015-02&rft.volume=42&rft.issue=2&rft.spage=400&rft.epage=407&rft.pages=400-407&rft.issn=1050-4648&rft.eissn=1095-9947&rft_id=info:doi/10.1016/j.fsi.2014.11.020&rft_dat=%3Cproquest_cross%3E1660398527%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1642618416&rft_id=info:pmid/25463292&rft_els_id=S105046481400446X&rfr_iscdi=true |