Development of species-specific primers for rapid identification of Debaryomyces hansenii

In this work, we developed a specific PCR assay for Debaryomyces hansenii strains that uses a putative homologous PAD1 region (729bp) present in this yeast species as a target. The amplification of this sequence with the D. hansenii specific primer pair (DhPADF/DhPADR) was found to be a rapid, speci...

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Veröffentlicht in:	International journal of food microbiology 2015-01, Vol.193, p.109-113
Hauptverfasser:	Wrent, Petra, Rivas, Eva-María, Gil de Prado, Elena, Peinado, José M., de Silóniz, María-Isabel
Format:	Artikel
Sprache:	eng
Schlagworte:	Debaryomyces Debaryomyces hansenii DNA Primers - genetics DNA, Ribosomal - genetics Food Microbiology - methods Foods PCR Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Rapid-identification Saccharomycetales - genetics Saccharomycetales - isolation & purification Sensitivity and Specificity Species Specificity Species-specific primers Yeasts
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