Rhodococcus soli sp. nov., an actinobacterium isolated from soil using a resuscitative technique

A Gram-positive, aerobic, non-motile, non-spore forming strain, designated DSD51Wᵀ, was isolated using a resuscitative technique from a soil sample collected from Kyoto park, Japan, and characterized by using a polyphasic approach. The morphological and chemotaxonomic properties of the isolate were typical of those of members of the genus Rhodococcus. Strain DSD51Wᵀwas found to form a coherent cluster with Rhodococcus hoagii ATCC 7005ᵀ, Rhodococcus equi NBRC 101255ᵀ, Rhodococcus defluvii Callᵀand Rhodococcus kunmingensis YIM 45607ᵀas its closest phylogenetic neighbours in 16S rRNA gene sequence analysis. However, the DNA–DNA hybridization values with the above strains were 58.2 ± 2.2, 58.4 ± 1.9, 45.1 ± 1.4 and 40.3 ± 4.7 %, respectively. In combination with differences in physiological and biochemical properties, strain DSD51Wᵀcan be concluded to represent a novel species of the genus Rhodococcus, for which the name Rhodococcus soli sp. nov. is proposed, with the type strain DSD51Wᵀ(=KCTC 29259ᵀ = JCM 19627ᵀ = DSM 46662ᵀ = KACC 17838ᵀ).